OS20 - Filtration assisted luminometric Elisa (fal-elisa) applied to the detection of foot-and-mouth disease virus non-structural proteins in formulated vaccines - C. Turco

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This document describes a study assessing the feasibility of using a Filtration Assisted Luminometric ELISA (FAL-ELISA) to detect foot-and-mouth disease virus (FMDV) non-structural protein 3B in formulated vaccines. The FAL-ELISA method was able to successfully detect 3B protein that had been spiked into vaccine samples after extraction of the aqueous phase. Validation studies showed the FAL-ELISA could reliably distinguish between positive and negative samples. The researchers concluded that FAL-ELISA has potential for replacing animal testing to evaluate FMD vaccines by allowing detection of non-structural proteins directly in formulated products.

Health & Medicine

1
Dra CECILIA TURCO
Team: Dra Florencia Mansilla, Dra María Cruz Miraglia and Dra Alejandra Capozzo
INTA and CONICET
Institute of Virology and Technical Innovations. Buenos Aires
ARGENTINA
FILTRATION ASSISTED LUMINOMETRIC ELISA (FAL-ELISA)
APPLIED TO THE DETECTION OF FOOT-AND-MOUTH DISEASE
VIRUS NON-STRUCTURAL PROTEINS IN FORMULATED VACCINES
AIM: assess the feasibility of detecting FMDV-3B protein in formulated
vaccines using the IPC FMDV kit
FAL-ELISA: principle
EuFMD | Open Session special edition | #OS20se
YY
Y Y YY Y Y
MONOCLONAL
ANTIBODY
anti-3B
SAMPLES (vaccine
extraction) and
CONTROLS
HRP Mab anti-
3ABC
Y Y Y
YY
LUMINOL/
PEROXIDE
SAMPLES DETECTION
20 min15 min 40 min
Y Y Y
EXTRACTION PROTOCOL PROOF OF PRINCIPLE: detection of NSP
in formulated vaccines
3ABC-spiked
vaccine
•Extraction of the aqueous phase from
the different vaccine preparations with
chloroform (1:1 V/V).
•Performed by triplicates during 2, 4 or
24h to evaluate 3ABC stability at 4°C
Quantitation
Sample RLU Result
Spiked 50 ng 364030 POS
Spiked 50 ng (1:2) 90620 POS
Spiked 10 ng 54780 POS
NIL 37480 NEG
Platevalidation
C30/NIL 4611,92 >20
C10/NIL 1519,05 >5
C30/C10 3,04 >1,5
3ABC in the aqueous phase extracted from spiked
FMDV-oil vaccines using FAL-ELISA
CONCLUSION
• FAL-ELISA could be used for the detection of FMDV non-structural proteins in formulated
vaccines.
• FAL-ELISA is a promising candidate for replacing the use of animals for the control of FMD-
vaccine purity, that can be particularly useful for vaccine banks and regulatory bodies.

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This document summarizes a presentation on alternative post-vaccination surveillance methods that could be used to demonstrate the absence of foot-and-mouth disease (FMD) virus transmission in vaccinated and unvaccinated livestock populations. It proposes replacing serological testing with bulk milk testing for dairy farms, saliva testing using rope tethers for piggeries, and saliva swab testing for sheep farms. These alternative methods utilize real-time reverse transcription polymerase chain reaction to detect FMD viral RNA from oral fluid samples, which research has shown can identify infected animals. The presentation discussed how these new testing technologies may allow countries to gain freedom from FMD status sooner after an outbreak by providing more effective post-vaccination surveillance.

LSD symposium - N. Galon - Thinking out of the pox lessons and thoughts on LS...

EuFMD

LSD symposium - C. E. Lamien - Molecular epidemiological investigation of LSD...

EuFMD

LSD symposium - I. Gluecks - Cattle farming in Kenya, Africa livestock health...

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LSD symposium - G. Pezzoni - Development of a pen-side test for the detection...

EuFMD

LSD symposium - A. Haegeman - LSDV transmission by stomoxys stable flies less...

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LSD symposium - W. Philips - Evaluation of the efficacy of live attenuated he...

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LSD symposium - A. Sprygin - Subclinical infection its role in transmission a...

EuFMD

The document discusses subclinical infection and its role in the transmission and epidemiology of Lumpy Skin Disease Virus (LSDV). It presents the body temperatures of experimental animals infected with LSDV over time. One animal showed clinical signs of LSDV while another showed viremia or presence of the virus in the blood without displaying clinical signs, representing a subclinical infection. The conclusion is that subclinical infection from vaccine-like recombinant LSDV can play a role in transmission of the virus.

LSD symposium - Z. Fatima- Investigation of suspected outbreaks of lumpy skin...

EuFMD

LSD symposium - L. Pite - Combating lumpy skin disease in Albania

EuFMD

1) The first case of Lumpy Skin Disease (LSD) in Albania was identified in June 2016. From 2016-2017, over 3,500 outbreaks were reported across Albania with morbidity of 42% and mortality of 12%. 2) Surveillance efforts included laboratory testing of over 2,000 samples confirming 881 positive cases. Risk factors for spread included proximity to infected farms (under 5km), livestock movements over longer distances, and seasonal variations correlated with temperature and vector abundance. 3) Control efforts included an emergency vaccination program using live attenuated vaccine beginning in July 2016. Over 500,000 vaccine doses were administered. Modeling estimated vaccine effectiveness was 76.5-62.5% at reducing

LSD symposium - E. Tuppurainen - Update on global distribution of lumpy skin ...

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LSD symposium - J. Chan - Lumpy skin disease in Hong Kong

EuFMD

Dr. Jason Chan presented on the outbreak of Lumpy Skin Disease (LSD) in feral cattle populations in Hong Kong from 2020-2021. The key points were: 1) The initial outbreak was reported in October 2020 across multiple country parks. Disease investigation found that 72% of cattle in one herd showed skin lesions and 84% were seropositive. 2) By March 2021, no new clinical cases were reported. Surveillance since found 14 juveniles seronegative, suggesting LSD may have disappeared due to lack of susceptible newborn cattle. 3) Continued clinical and serological surveillance is important since Hong Kong has a small teaching farm. No urgency exists currently to declare freedom

LSD symposium - N. Zainuddin - Indonesian experience on simultaneous LSD and ...

EuFMD

1) Lumpy skin disease was first reported in Indonesia in February 2022 in Riau Province, and has since spread to several other provinces, most recently to Central Java in August 2022. 2) As of February 2023, over 249,000 cattle have been vaccinated across 9 provinces as a control measure. Other control measures include movement restrictions, vector control, and educating farmers. 3) Key challenges to control efforts include the extensive animal farming system, illegal animal movement, limited number of vaccinators, and high workload from controlling both lumpy skin disease and foot-and-mouth disease. Recommended solutions include improving handling capacity, better border control, engaging other institutions to assist with vaccination

LSD symposium - R. Ainsworth - Lumpy skin disease (LSD) in Southeast Asia Mar...

EuFMD

Lumpy Skin Disease (LSD) is spreading through cattle movements in Southeast Asia. The document discusses how government policies around quarantine, compensation and corruption can accelerate the virus's spread by encouraging illicit cattle movements. It also notes that traditional smuggling routes go against the direction LSD has spread. The rapid transmission of LSD occurred during COVID border closures, and its direction of movement corresponds with prevailing winds rather than cattle trade routes. Government policies and wind patterns may be aiding the long-distance airborne spread of LSD across Southeast Asia.

LSD symposium - P. Malik - Lumpy skin disease experience from India

EuFMD

Lumpy Skin Disease (LSD) was first reported in India in 2019. It has since spread to 23 states and union territories, affecting over 3 million animals and causing over 185,000 deaths. The disease manifests as skin nodules and lesions on internal organs. Vaccination is a key control strategy, with over 87 million animals vaccinated to date using a goatpox vaccine. ICAR has also developed an indigenous LSD vaccine that is undergoing field trials and licensing. States are implementing control measures like quarantine, vaccination, vector control and public awareness campaigns to curb the spread and impact of LSD.

LSD symposium - G. Cattoli - Lessons from ten years of experience building me...

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LSD symposium - H. Bergmann - Systemic review and expert ranking of potential...

EuFMD

LSD symposium - E. Klemen - Modes of transmission of lumpy skin disease

EuFMD

Indirect transmission, likely through blood-sucking flying insects, is the primary mode of transmission for lumpy skin disease virus. While direct contact can transmit the virus, studies have found no transmission between clinically infected and susceptible cattle housed together without vectors. Mathematical models also indicate indirect transmission alone can explain outbreak dynamics. The virus can spread over long distances, possibly aided by winds carrying infected vectors, though local spread is typically 10 km per week. Subclinical infections may transmit the virus but appear to play a minor role compared to clinical cases.

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