This document describes the services provided by a cell culture laboratory, including maintaining various mammalian and invertebrate cell lines, characterizing new cell lines, freezing and thawing cell lines, and evaluating contamination. The laboratory isolates and identifies various viruses, parasites, and other pathogens in cell cultures and animals. It also develops vaccines against pathogens like Coxiella burnetii, Chlamydia, and Cryptosporidium using infected cell cultures. The laboratory produces medium and large scale infected cell cultures for vaccine production.

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Infrastructure and equipment of the laboratory for handling cell cultures.
Obtaining and production of primary cultures from any organ of human or animal species.
Maintenance of mammalian cell lines (VERO, Hep2, McCoy, BHK21, RFT18, CACO2MDBK, MDCK, etc.)
Maintenance of invertebrate cell lines.
Karyotypic characterization of cells in culture (modal class).
Evaluation and Control of contamination in culture rooms and cells ( viruses, Mycoplasma sp., Fungi).
In Vitro cell transformation.
Freezing and thawing of cell lines (Cell line bank).
Maintaining of Myelomas and Hybridomas cell lines for (monoclonal Abs bank).
Establishment and characterization of new cell culture lines.
Multiparameter studies, viability and apoptosis by flow cytometry.
Production to median and large-scale (Rolex, fermenters ).
Study of cell ultrastructures by transmission electron microscopy.

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Method for scrubbing of tissue culture glassware using trisodium phosphate (1980)
BB82. New line cell culture for virus studies (1982)
Obtaining primary cell cultures from cells and recovered media (1983)
Obtaining and characterization of cell culture line PILI001, persistently infected with Lawsonia
intracellularis. New biological system for the production of vaccines. (2001)
COE (Lamb umbilical cord cells). New cell Line continues for studies of Protozoa (2009)
MONO. Bovine Mononuclear diploid cell line, obtain by clonal cell expansion by Theileria annulata.
(2010).

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Isolation and identification of Astrovirus
Isolation and identification of Rotavirus type A
Isolation and identification of Pestivirus (BVDV, BDV, CSFV)
Isolation and identification of BHV type I
Isolation and identification of BHV type II
Isolation and identification of Bovine Leukemia virus ( BLV)
Isolation and identification of Equine Anemia virus (AIEV)
Isolation and identification of Smallemberg virus (VSB)
Isolation and identification of PRRSV
Isolation and identification of BRSV
Isolation and identification of Feline Calicivirus
Isolation and identification of Feline Parvovirus (FPV)
Isolation and identification of Canine Parvovirus (CPV)
Isolation and identification of Canine Coronavirus (CCoV)
Isolation and identification of Feline Coronavirus (FCoV)
Isolation and identification of Canine Distemper (CDV)
Isolation and identification of Caprine Herpesvirus (CpHV-1)
Isolation and identification of Para Influenza III (PI 3)
Isolation and identification of Porcine parvovirus (PPV)
Isolation and identification of Porcine Circovirus (PCV-2)

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Isolation and identification of Aujeszky disease (PHV-I) in cell culture and laboratory animals
Isolation and identification of Encefalomiocarditis virus (EMCV) in cell culture and laboratory animals
Isolationand identificationofAvíanInfectiousBronchitis (AIBV) in cell culture and chicken embryos
Isolation and identification of Newcastlediseasevirus (NDV) in cell culture and chicken embryos
Isolation and identification of Coxiella burnetii in cell culture and chicken embryos
Isolation and identification of Chlamydias and Chlamydophilas in cell culture and chicken embryos

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Isolation and identification of Theileria annulata from clinical samples.
Culturing and identification of Cryptosporidium sp. from clinical samples.
Isolation and identification of Encephalitozoon cuniculi from clinical samples.
Isolation and identification of Babesia sp (B. bovis and B. bigemina) from clinical samples.
Isolation and identification of Anaplasma marginale from clinical samples.
Isolation and identification of Toxoplasma gondii. from clinical samples .cell culture and mouse.
Isolation and identification of Besnoitia besnoiti from clinical samples.
Isolation and identification of olation and identification of Eimeria sp. from clinical samples.
Isolation and identification of Trypanosome sp. from clinical samples
Maintenance and passage of Leishmania infantun strain in cell culture.

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Production of medium-scale of infected cell cultures for the production of viral vaccines
Recombinant antigens FOR PRODUCTION OF VACCINES
Design and development of a vaccine against Coxiella burnetii in VERO cell line
Design and development of a vaccine against Chlamydia sp. in Mc Coy cell line
Design and development of a vaccine against Bovine Tristeza from cell cultures infected ticks
Development of Cryptosporidium sp vaccines in COE cell line
Development of Encephalitozoon cuniculi vaccines in COE cell line
Development of Besnoitia besnoiti vaccines in COE cell line
Development of Toxoplasma gondii vaccines in COE cell line
Development of Babesia sp vaccine in primary culture from peripheral blood of infected cattle
Development of Anaplasma marginale in primary culture from peripheral blood of infected cattle
Development of primary cultures of infected lymphocytes Theileria annulata for obtaining vaccines
Production of medium-scale of Brachyspira sp. autovaccines
Commissioning and production of bacterin for pathogenic Leptospira.