This document contains the curriculum vitae of Panagiotis Alexandros Paralis. It details his education in biomedical sciences from Oxford Brookes University in 2006. It then outlines his relevant work experience, including roles at Oxford University Hospitals from 2008-2013 where he gained experience in various laboratory roles and techniques. Most recently, he has worked as a biomedical scientist in diagnostic centres in Thessaloniki, Greece since 2015.
This is question one from my media evaluation work. I have created three elements of a pop music magazine, and this PowerPoint analyses how it differs and compares with a professional magazine.
Investigate archaea with the Eurofins Genomics Microbiome Profiling 3.0 service! In addition to profiling bacteria and fungi, we now offer profiling of the archaea community in your samples. Why did we include archaea? It has been found that archaea are associated with conditions such as irritable bowel disease, obesity, anorexia nervosa, but also with infectious diseases like brain abscesses. Methanogenic archaea in the digestive tract of ruminants are the main producers of the greenhouse gas methane. Archaea also play roles in the microbiomes of the human skin and mouth, they show growth- and health-promoting effects on plants, and are present in the microbiome of corals, intracellular niches in amoebae, protozoa, and termites. The option to profile archaea provides a tool for researchers to broaden the knowledge about the effects and impact of archaea.
A study of antibiotic resistance of Extended-Spectrum Beta-Lactamases produci...Premier Publishers
Background: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are common in hospitals. This study aims to describe the antibiotic resistance of these bacteria and their associated demographic and clinical factors. Methods: It was a prospective study of 73 isolates of Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae for a period of six months from July to December 2019 in the laboratory of Befelatanana. Results: This study showed 73 (6.3%) isolates of Extended-Spectrum Beta-Lactamases- producing Enterobacteriaceae, represented by 25 (34.2%) isolates of Klebsiella spp, 24 (32.9%) isolates of Escherichia coli, 22 (30.1%) isolates of Enterobacter spp and 2 (2.7%) isolates of Proteus spp. The antibiotic resistance of these bacteria varied from 0% to 100% for all of the antibiotics tested. Resistance to aminoglycosides ranged from 0% (amikacin) to 69.9% (gentamycin). Resistance to quinolones ranged from 43.8% (levofloxacin) to 76.7% (nalidixic acid). Similarly, 60 (82.2%) isolates were resistant to cotrimoxazole and 25 (34.2%) isolates to chloramphenicol. Patients under 20 years (57.1%) (p=0.03), men (52.2%)(p=0.11; NS), patients with respiratory samples (83.3%)(p=0.004), with pus (61.9%)(p=0.02) and hospitalized in surgery and intensive care units (68.4%)(p=0.0009) were the most affected by these enterobacteria. Conclusion: Extended-Spectrum Beta-Lactamases - producing Enterobacteriaceae are responsible for severe infections and the majorities are multi-resistant bacteria.
Keywords: Beta-lactamase, Enterobacteriaceae, antibiotic resistance, amikacin, imipenem.
Bio303 laboratory diagnosis of infectionMark Pallen
In this Bio303 module talk, I provide an overview of how infections are diagnosed in the clinical microbiology lab, focusing on technologies, old and new, and also on practical issues and workflows crucial to optimal use of the lab.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Current CV .
My objective is to obtain a rewarding and challenging research scientist position where my background and experience will contribute to the success of a growing company or research center.
Currently, I am a Senior Associate Scientist at Amgen Inc. and certified Molecular Biologist with the American Society of Clinical Pathology MB (ASCP). I have more than 10 years of experience in the biotechnology/ pharmaceutical industry. I am highly proficient in various lab techniques, technologies, and automation. I demonstrated consistent success in the execution of assay development and method validation activities supporting clinical stage programs within GCP and GLP regulated environments. I possess extensive experience in optimization and validation of drug potency assays (ELISA and cell based assays), protein purification and characterization, and DNA/RNA extraction and quantitation. I am a subject matter expertise in the areas of human and rodent cell lines propagation and tissue dis-aggregation. I have proven operational capabilities in the establishment of standard operating procedures to ensure our laboratory meets regulatory and business requirements.
I am a self-motivated professional who works effectively as an individual contributor or within a team matrix. As a quick learner, I can efficiently deliver results, easily adapt to changing environment and provide fresh ideas. My strengths include statistical analysis/guidance, report writing, and communication.
Thank you in advance for your consideration. Please feel free to call me at (805-990-6258), or by e-mail at (mahawally46@gmail.com) if you have questions or would like a list of references.
Sincerely,
Maha Rizk
1. Name: Panagiotis Alexandros Paralis
Current Address: Triantafillopoulou 26, Pylaia, Thessaloniki, Greece, PC: 54352
Email: panosparalis@btinternet.com
Alternative email: panosparalis@gmail.com
Skype: panosparalis1
Linked in profile: https://www.linkedin.com/pub/panagiotis-paralis/44/aa3/aa
Website: http://panagiotisparalis.wix.com/panagiotisparalis
http://alex2007.wix.com/curriculum-vitae-r
CURRICULUM VITAE
EDUCATION
2006: BSC HONS DEGREE BIOMEDICAL SCIENCES, OXFORD BROOKES UNIVERSITY
Topics Covered During Degree
Word-Processing and Spreadsheet IT skills
Microbiology and Immunology (double module); Cell count
Laboratory Techniques for the Biosciences (double module); Animal cell culture; Sf9 insect
cells, handling a suspension culture; Mouse 3T3 cells, handling a monolayer culture.
Estimation of Na+ and K+ in plasma and red cells; Operating fluorescence
spectrophotometer, UV-2501PC double beam spectrophotometer.
Clinical Biochemistry and Haematology (Honours Component); Case study of liver diseases.
Clinical Immunology and Microbiology (Honours Component); perform of oxidase, catalase,
API 20E tests, DNAase, in a variety of selective and non-selective agar plates.
Biomedical Science Project (double Honours Component). Campylobacter spp. detection by
ELISA directly from human faeces. (Work associated with the Oxford University Hospitals)
Clinical Pharmacology (double module)
Molecular Biology of Cancer (Honours Component)
2. Cellular Pathology (Honours Component); types of fixation, and stains in human tissues,
microscopic examination and interpretation of carcinoma in uterus.
RELEVANT WORK EXPERIENCE
OCTOBER 2008- AUGUST 2009: OXFORD UNIVERSITY HOSPITALS NHS
Laboratory support worker
Duties included:
Core computer activities
Mailbox
Order entry
Microbiology inquiry
Administrative data entry
Credit test
Core laboratory activities
Knowledge & Understanding of risks associated within all the sections
Core skills
Communication, verbal and written accurate messages
Health and safety
Team working
Autoclave room
Knowledge & Understanding of risks associated with waste disposal including category 3 organisms
Specimen reception room
Knowledge & Understanding of all the type of specimens associated with a microbiology department
Urine section
Excellent Knowledge & Understanding of the procedures followed within the section
Pus bench section
Set up surface cultures
Genital cultures
Pus- PMC
3. Sterile specimens
Blood culture section
Line tip culture
Bactec FX system use for blood bottles culture
Mycology and faeces section
Set up Faeces, and DRMC cultures
Virology – Serology section
Use of the SDS Viper for Chlamydia detection from swabs
Screening section
Set up MRSA screening, ACSC
SEPTEMBER 2009- APRIL 2013: OXFORD UNIVERSITY HOSPITALS NHS PROMOTED AS A BIOMEDICAL
SCIENTIST TRAINEE A
Duties included:
Core computer skills
Reporting results
CORE LABORATORY ACTIVITIES
Knowledge from benches reading and culture for bacterial identification in particular
SFC- Surface site cultures for bacterial and fungal investigation of swabs from superficial and
nonsurgical sites, for the diagnosis of infection.
MRSA-Screening test is carried out to identify colonisation with Methicillin Resistant
Staphylococcus aureus (MRSA) in patients. Swabs taken from nose and other body surfaces
are screened for MRSA using selective and enrichment media.
Urines-Urinary tract infections, bacterial identification from BD CHROMagar Orientation
medium.
Faeces-Investigation of samples, mainly stools, for the detection and identification of a range
of ova, cysts, parasites, cells and abnormalities from enteric pathogens.
PMC- Pus: Microscopy, Culture Bench for bacteriological investigation of tissue, fluid and
swabs from abscesses, deep seated sites and normally sterile fluids, to diagnose infection.
STER- Bacteriological investigation of tissue, bone and joint fluid samples from prosthetic
and native joints for the diagnosis of infection. Specimens processed include
kidney/pancreatic perfusion fluids, donor bone graft samples and corneal donor grafts.
GENC- Genital cultures for bacteriological, fungal and parasitic investigation of male and
female genital specimens for the diagnosis of genital and sexually transmitted infection.
Mycology: Dermatophytes for isolation and identification of dermatophytes in human
epidermal tissue, usually skin, nail or hair.
4. CULTURES, MANUAL TESTS
Processing the vast majority of the clinical specimen received in microbiology department including
category 3 microorganisms such as Mycobacterium tuberculosis, Salmonella typhi (one year of
experience in containment level 3 laboratories). Perform all manual test within the microbiology and
serology such as ASOT, Binax NOW Legionella Urinary Antigen rapid test, Brucelloslide test,
Cryptococcal Ag test, Binax NOW RSV rapid test for Respiratory Syncytial Virus, API’s, Group Testing
Kits, and Staph Latex Kits.
MICROSCOPIES
Acridine orange: Pus cells, Yeasts, Clue cells, and Trichomonas identification.
Gram stain: Pus Cells, Yeasts, and bacterial identification depending on their Gram
morphology.
Ziehl–Neelsen: Mycobacterial Tuberculosis identification
Haemocytometer C-Chip: Cell count and cell differentiation from Cerebro-Spinal Fluid or
deep abscess fluids.
CLINICAL LABORATORY INSTRUMENT USER:
ETIMAX- automated immunoassay system.
IL Biokit Best 2000- automated immunoassay system.
DiaSorinLIAISON-automated Chemiluminescence analyser.
Dynex DS2-automated immunoassay system.
BD Viper™ System with XTR™ - molecular diagnostics of Chlamydia trachomatis/
Neisseria gonorrhoeae (CT/GC) testing.
Becton Dickinson BACTEC FX system-blood cultures and sterile site cultures.
BACTEC® MGIT™ 960-in vitro diagnostic instrument designed and optimised for the rapid
detection of mycobacteria from clinical specimens.
GeneXpert Dx system-nucleic acid amplification and detection of the target sequences in
simple or complex samples using real time PCR and reverse transcriptase PCR. In particular,
the Xpert Flu Assay is a rapid, automated in vitro diagnostic test for qualitative detection of
Influenza A, Influenza B, and Influenza A subtype.
Urisys 2400 and 1800 Roche (in Greece)
XT 2000i and 1800i Sysmex Haematology Analyzer (in Greece)
BCS XP system analyser(in Greece)
Elecsys cobas ROCHE e411(in Greece)
CORE SKILLS
Health and safety, Quality procedures, working on the IBMS portfolio
Team working, and excellent self-motivation
5. NOVEMBER 2013- DECEMBER 2013: QUALITY MANAGER AT LAZOS MEDICAL COMPANY
Duties included:
Quality assurance for mass production of microbiological agar plates, nutrient broths, stains,
gels, and chemicals.
Quality control for bacterial contamination and decontamination of the media produced by the
company.
Produce of chemicals, stains, nutrient broths, and gels.
Use of the autoclave and Systec media prep 30 for mass production of agar
Writing up of protocols for ISO preparation.
SEPTEMBER 2014- JANUARY 2015 VOLUNTEER WORK: PHLEBOTOMIST AT 424 MILITARY HOSPITAL IN
THESSALONIKI
Drawing blood from patients
OCTOBER 2014 – JUNE 2015: LECTURER AT D.I.E.K THESSALONIKI FOR THE COURSE OF MEDICAL
LABORATORY ASSISTANTS
Lecturer in theoretical and practical aspects for the module disinfection and introduction to
laboratory instruments
Lecturer in practical aspects for the module microbiology
JANUARY 2015 – TODAY: BIOMEDICAL SCIENTIST IN ANALYSIS RESEARCH AND DIAGNOSTIC CENTRES IN
THESSALONIKI
Dealing with phlebotomy
Culture from biological fluids and tissues (human and animal) on media agars from a variety
of clinical specimens according to the laboratory protocols
Clinical instrument user (Elecsys cobas Roche, Urisys 1800, BCS XP system haemostasis,
XT 2000i Sysmex) and manual test proceed (e.g Brucella test, Coombs direct and indirect,
F.O.B test , Mononucleosis test, RPR test, Strep test, Systemic lupus erythematosus test,
VDRL test, Blood type and Rhesus typing kit)
Translate scientific articles on behalf of the company for the company’s social media
Main responsible for sample archiving and turn around time of the specimens
QUALIFICATIONS AND SKILLS
Overseas sub degree – Kastoria Greece
Bachelor hons in Biomedical Sciences- Oxford Brookes University in UK
ICT Europe certificate intermediate b- Office 2007 in word, Excel, Power point, Internet
IELTS certificate –British council
ISO 17025 certificate- Aristotle University in Thessaloniki Greece
Member of the Panhellenic Association of Medical Laboratory Technologists –P.E.T.I.E,
member of the IFBLS and EPBS, membership number 1453
Ability to pick up new packages quickly and with the minimum training