The document summarizes information about stem cells and stem cell banking services provided by Cryo-Save India. It describes the types of stem cells in the human body, sources of stem cells like cord blood, bone marrow and umbilical cord, and the potential of these stem cell sources for regenerative therapies. It also provides details about Cryo-Save India's stem cell collection, processing and long-term storage services.

1. Cryo-save India (part of Cryo-Save NV) Pune Office Shop No 3, Princeton Flair Society, Lane No 06, Koregaon Park Pune411001 Stem cells Banking

2. We all have stem cells. They are essentially in those parts of the human body that are designed to make running repairs. When you cut your finger, stem cells get to work to repair your broken skin. When you think of those parts of our bodies that can repair themselves: skin, bone, muscle, it's because of stem cells. Basics of stem cells

4. 4.Cord Blood Stem Cells 1.Bone Marrow Stem Cells 2.Embryonic Stem cells 3.Peripheral Stem Cells ( Fat, Brain, Nose, Skin, teeth, Blood ) 5.Cord Lining Stem Cells What are sources of stem cells in the Human Body?

5. Cryo-save India (part of Cryo-Save NV) The cord blood is rich in residual cells which were not used in developing the fetus. U Cord stem cells are called Naïve Stem cells. Cord blood is now being used increasingly on an experimental basis as a source of stem cells, as an alternative to bone marrow. Why stem cells from U cord Blood ?

7. 1 in every 300 of the jelly cells is a stem cell. 1 out of 200 million cord blood cells is a stem cell As it is a new source harvesting is slow and manual, requires in house R&D for proper harvesting techniques Harvesting is easy and well defined can differentiate into a variety of cell types can differentiate into blood and immune cells Rich in Mesenchymal Stem Cells Rich in Hematopoietic Stem Cells Cord lining Cord Blood

8. Time Vs transplant Matching probability Time Vs Availability Quantity Source Vintage New source of stem cells Since 1975 Old source of stem cells In use since 1950’s Once frozen, a Sample is always available for the Family Donor must be found and confirm to the HLA typing 100% match for the child an 25% match for family members per sample Requires a perfect match When a match is found, can take only a few days for confirmatory and special testing and shipment to the Transplant Center. After a formal search is begun, takes an average of 4 months to transplant, if a donor is available. Larger amount of stem cells are harvested from Cord Blood + Cord Lining 7.5 – 25 x 10 ⁹ /ml Approx 2 - 5 x 10 ⁹ / ml Or 2% of a persons bone marrow Harvested for Umbilical Cord (bio Medical waste) No harm to Mother or child Harvested from pelvic / hip joint Takes 4-6 hrs with side effects Umbilical Cord Bone marrow

9. After Delivery the cord is Clamped, cut and child is separated Collection process

10. Cryo-save India (part of Cryo-Save NV) Maternal Blood Sample Tube Collection process Cord Lining Tube Cord Blood Bag Kit Box

12. Vacuum insulated double hull tank Storage using liquid nitrogen at -196°C Bar coded Cord Blood Stem Cell Sample storing of stem cells

13. Cryo-save India (part of Cryo-Save NV) Therapy Pharmaceutical Stem cell Therapy Surgery

14. Cryo-save India (part of Cryo-Save NV) 1. All diseases cured using stem cells are clinical trails and not therapies 2. Stem Cell therapy as a 3 rd alternative method of cure will take time. 3. Therapy procedures using stem cells are yet to be defined, published in medical journals and recognized by the governing bodies. 4. It is currently as a supportive therapy. Clarity on Stem Cell Therapy

15. In many diseases, cells are dead or damaged. Researchers are exploring ways of using stem cells to produce cells which could replace these. This is basically called as « REGENERATIVE THERAPY » Much research is currently focused on using new heart muscle cells to help heart failure patients, and on producing new brain neurons to treat people with Parkinson's or Alzheimer's diseases . How is Therapy done using stem cells

16. Therapy Focus

17. The first successful cord blood transplant was performed in 1988 on a 5 year old boy with Fanconi’s Anemia (a rare, genetic and lethal type of anemia) He was given 0% chance for survival. The donor was his newborn sister. Today, he is alive and free from disease more than 20 years after the transplant. In February 2007 a child suffering from a severe T-cell (CD8+) immune deficiency syndrome has been treated successfully with umbilical cord blood stem cells in Portugal. The stem cells used for this transplantation were h arvested from the umbilical cord blood . The umbilical cord blood sample was processed and stored at the private stem cell bank facilities of Cryo-Save in Belgium. History of Stem cell Therapy

18. Cryo-save India (part of Cryo-Save NV) Treatment of heart problems using stem cells was first in March 2005 by AIIMS in New Delhi when trials were conducted on 35 patients. There was no mortality reported and all patients were brought in at a stage where surgery was ineffective. After six months, 56 % of the affected area injected with these cells showed improvement. After 18 months, this went up to 64% Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India

19. Cryo-save India (part of Cryo-Save NV) Manipal Hospital discovers cure for Parkinson`s disease ( Apr. 04th., 2007) Mr Andrew Kisana, a 58-year-old patient affected by Parkinson’s disease has shown positive signs of cure with stem cell therapy. With the success in treating this patient, India-based Manipal Hospital has proved that stem cell therapy could be a potential option for easing out symptoms in a patient suffering from Parkinson’s disease. Parkinson’s disease is a neuro-degenerative disease that affects people over the age of 50. Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India

20. Cryo-save India (part of Cryo-Save NV) Four-month-old Aarohi Bhatt recently created medical history for being cured of a rare heart condition believed to be untreatable. The cause of her cure—Stem Cell Therapy using her father's blood at Frontier Lifeline Hospital, Chennai. Besides being a difficult surgery, it was for the first time in the world that SCT was successfully done on a child so young. Lifeline Hospital, Chennai, 2005 Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India

21. Cryo-save India (part of Cryo-Save NV) "This is the first time that Indian doctors have resorted to SCT to cure spinal cord problems and the second time in the world of literature," said Dr JS Rajkumar, Chief Surgeon, Lifeline Multi-Speciality Hospital,Chennai Ali underwent autologous SCT in December 2006. The Team aspirated 100ml of bone marrow fluid from the hip bone, isolated the stem cells, and 20 ml of this concentrate was injected into his spinal fluid. Soon, he was able to walk on his own. Lifeline Multi-Speciality Hospital,Chennai December 2006 Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India

22. Six Year Old Sumaiya Aziza’s eye was damaged when a packet of wet lime or chuna had exploded into her eyes her parents had come to the L.V. Prasad Eye Institute (LVPEI) in Hyderabad in August 2001. A special form of cornea transplant was performed on her damaged eye. It was a historic first in India: Dr Virendra Sangwan simply took a small piece of limbus (border of cornea) from Aziza’s healthy right eye, cultured it on an amniotic membrane in the lab and after 11 days put it back into her wounded left eye. The techniques he used were “indigenous, innovative and low-tech”. By 2004, when President A.P. J. Abdul Kalam visited the eye institute, Aziza had near-normal vision with her glasses. Kalam was so impressed that he later invited a senior surgeon of the hospital, Dr Taraprasad Das, now working on retina reconstruction with stem cells, to join him on his East Asia tour to speak of India’s stem cell success story Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ L.V. Prasad Eye Institute August 2001

23. Hospitals offering Stem Cell Therapy (India) Cardiac, Cancer Apollo Hospital Cardiac Life Line Hospital Cardiac, Ophthalmology, Neurosurgery, Vascular Surgery, Diabetes, Cancers Manipal Hospital, Bangalore Cancer TATA Memorial Ophthalmology L V Prasad eye inst Cardiac, Diabetes Christian College Vellore Cardiac, Neuro Surgery, Vascular Surgery, Diabetes, Ophthalmology, cancer AIMS Delhi Area of Specialization Hospital

24. Europe’s Leading Stem Cell Bank Now in India Company Profile

26. People Involved Working in stem cells for over 20 years, he is an opinion leader who has been called upon by governments and hospitals, including the US Senate, the United Nations (Geneva) and the Parliaments of France, UK, Austria and Germany. Know for creating the first liver cell from cord stem cells Professor Colin McGuckin Scientific Director Cryo-Save Group Doctor Rob Koremans MD and CEO of Cryo-Save Group VR Chandramouli, MD, Cryo Save India

27. Scientific Approach ITERA (International Tissue Engineering Research Association Cryo-Save is a founding member of ITERA Forum, an international forum of scientists specialising in stem cells, tissue engineering and regenerative medicine it is dedicated to the latest developments in stem cell research . CRYSTAL (Cryo banking of Stem cells for human Therapeutic Application) Together with 5 universities in Europe and the Fraunhofer Institute, Cryo-Save has been selected to conduct fundamental research in the field of cryo preservation techniques for stem cells

28. Situated in Whitefield, Bangalore conforms to GLP Area of 10,000Sqft. which can store initially 1,50,000 samples which will make it the largest such storage facility in Asia. Cryo-Save India

29. Cryo-Save India (Storage Facility)

31. Registration Process Registration Kit Box Delivery Collection Dispatch Intimation to Doctor Paramedic Doctor

32. Post Registration procedure Sample Received at Lab Sample Processing Maternal Sample Inspection Cryo preservation Final Report to Client

34. I SAVED Client name – Trilochana Patel Name - Date of delivery- 14/02/2009 Hospital- Meera hospital Doctor- Dr. Umranikar “ we were happy with the way presentation was given, and they were very transparent in all their information on stem cells

37. Cryo-save India (part of Cryo-Save NV) Payments and refund 3 PAYMENT OPTIONS 11 PDC’s of Rs 5000 25,000 Standard Option 3 CS08003 3 30,000 20,000 25,000 Standard option 2 CS08002 2 75,000 Standard option 1 CS08001 1 Third Payment Second Payment First Payment Plan options Plan Ref Sr No.

38. All the best