Stem cells are cells that can develop into many different types of cells. The document discusses sources of stem cells like bone marrow, umbilical cord blood, and embryonic stem cells. It provides information on Cryo-Save India which collects and stores umbilical cord blood and cord lining stem cells to be used for transplants and therapies. Cryo-Save India is part of a large international stem cell banking company and provides affordable stem cell storage and banking services in India.

1. Cryo-save India (part of Cryo-Save NV) Stem cells Banking

2. WHAT ARE STEM CELLS? "Stem cells are cells that have the potential to grow into any other type of cells“ They are the building blocks of the blood and immune system that can reproduce into other cell types, including bone, heart, muscle, and nerve.

3. What are sources/types of stem cells in the Human Body? 3.Blood Stem Cells 1.Bone Marrow Stem Cells 2.Embryonic Stem cells 4.Cord Lining Stem Cells

4. Cryo-save India (part of Cryo-Save NV) Why stem cells from U cord After a baby is born and the umbilical cord is cut, some blood remains in the blood vessels of the placenta and the portion of the umbilical cord that remains attached to it. After birth, the baby no longer needs this extra blood. This blood is called placental blood or umbilical cord blood: "cord blood" for short. Cord blood contains all the normal elements of blood - red blood cells, white blood cells, platelets and plasma. But it is also rich in hematopoietic (blood-forming) stem cells, similar to those found in bone marrow. This is why cord blood can be used for transplantation instead of bone marrow. Cord blood is being used increasingly on an experimental basis as a source of stem cells, as an alternative to bone marrow. Most cord blood transplants have been done to treat diseases of the blood and immune system. It has also been used to restore the functional deficiencies of several genetic metabolic diseases. To date, more than 70 different diseases have been treated with cord blood transplants.

5. bone marrow cord blood Donation requires surgery under general anesthesia. Donors may experience temporary discomfort and/or pain. Long-term consequences of growth factors used in peripheral blood stem cell donations are uncertain. When obtained from the delivered placenta and umbilical cord, donation poses no risk to mother or infant. Requires donation of a quart or more of bone marrow (mixed with blood) for transplant A few ounces can be used for transplantation. Large dose of stem cells. Rapid engraftment. Smaller dose of stem cells. Slower engraftment Potential donors may no longer be available or may have withdrawn consent. Donor must be found and retested to confirm the HLA typing and infectious disease results and to confirm that the donor is still willing and able to donate bone marrow. Significant donor attrition Once frozen, a cord blood unit is available until used. Otherwise, no donor attrition Generally requires a perfect match between donor and recipient for 6/6 HLA Generally requires a match between donor and recipient for 4/6 ,5/6 ,6/6 HLA After a formal search is begun, takes an average of 4 months to transplant, if a donor is available. When a match is found, can take only a few days for confirmatory and special testing and shipment to the Transplant Center (less than 24 hours in an emergency).

6. Cryo-save India (part of Cryo-Save NV) Collection and Processing of cord blood and cord lining stem cells

7. Collection process After Delivery the cord is clamped, cut and child is separated

8. Cryo-save India (part of Cryo-Save NV) Collection process Cord Lining Tube Cord Blood Bag Maternal Blood Sample Tube Kit Box

9. storing of stem cells Vacuum insulated double hull tank Inventory system allows for easy retrieval of individual units Storage set up with breakdown back-up systems

10. Cryo-save India (part of Cryo-Save NV) Therapy using stem cells and Cord Blood Stem Cells

11. Describe HLA matching The human leukocyte antigen (HLA) test, also known as HLA typing or tissue typing, identifies antigens on the white blood cells (WBC’s) that determine tissue compatibility for organ transplantation . (that is, histocompatibility testing). Similar to blood grouping i.e. A, B, AB IN SIMPLE TERMS ITS PROTEIN MATCHING OF 6 PARAMETERS. In case of bone marrow we require 100% match (i.e. 5/6 or 6/6) 50% match in case of cord stem cells.( i.e. 3/6 OR 4/6)

12. How is Therapy done using stem cells

13. Real people, real stories..! The most precious gift! Proven therapy 06/16/09 Cryo-save India (part of Cryo-Save NV)

14. 06/16/09 Cryo-save India (part of Cryo-Save NV) What do these people have in common ? Nicholas Henderson – T cell Lymphoma Austin – Myelo Dysplastic Syndrome Tom Stretch – Chronic Granulomatous Disease Hemant Bebera - Thalassemia Blayke & Garrett LaRue – X Linked Lympho-proliferative Disorder Erica Nader – Spinal cord injury Hank Heacock - Leukemia All these patients were treated with Stem Cells at various centres.

15. Cryo-save India (part of Cryo-Save NV) Therapy in India AIIMS team shows how stem cells save hearts. Taken from bone marrow, they can revitalize non-functional heart muscles.

16. 06/16/09 Cryo-save India (part of Cryo-Save NV)

17. The first successful cord blood transplant was performed in 1988 on a 5 year old boy with Fanconi’s Anemia (a rare, genetic and lethal type of anemia). He was given 0% chance for survival. The donor was his newborn sister. Today, he is alive and free from disease more than 15 years after the transplant. In February 2007 a child suffering from a severe T-cell (CD8+) immune deficiency syndrome has been treated successfully with umbilical cord blood stem cells in Portugal. The stem cells used for this transplantation were h arvested from the umbilical cord blood . The umbilical cord blood sample was processed and stored at the private stem cell bank facilities of Cryo-Save in Belgium.

18. CCMB, LVPEI and Sarojini Eye Hospital- We dnesday, March 02, 2005 06/16/09 Cryo-save India (part of Cryo-Save NV) 250 patients who had undergone stem cell therapy - 70 % had vision restored. The next step –Develop a hemi-cornea Final goal- Reconstruct an entire cornea from a suitable stem cell source CCMB eyes on creating cornea in-vitro

19. 06/16/09 Cryo-save India (part of Cryo-Save NV)

20. January 10, 2009 The Times of India.

21. Some Transplant Centres in India Tata Memorial Hospital, Mumbai Adyar Cancer Centre, Chennai Apollo Specialty Hospital, Chennai Apollo Hospital, Hyderabad Christian Medical College, Vellore R&R Army Hospital, New Delhi AIIMS, New Delhi Inlaks Hospital, Pune Armed Forces Medical College, Pune Sanjay Gandhi PGIMS, Lucknow Cryo-save India (part of Cryo-Save NV)

22. Most recent applications (India) 1.Diabetes (National Centre for Cell Sciences, Pune University – Dr. Ramesh Bhonde) 2.Heart (Dr. Venugopal Rao – AIMS) 3.Liver Disease 4.Parkinson's Disease (Manipal Dr. Satish Totey) 5.Spinal Cord Injury (AFMC, Pune) 6.Stroke 7.Alzheimer's Disease

23. Cryo-save India (part of Cryo-Save NV) Company Profile

24. Europe’s Leading Stem Cell Bank Now in India

25. Cryo-save India (part of Cryo-Save NV) Cryo save group Holding established in 2000 in the Netherlands Headquarters in Switzerland Profitable, debt-free and cash generative Operates in 36 countries from four operating Repositories MNC – Listed in London stock exchange Cryo-Save is the largest stem cell storage company in Europe and the fourth biggest in the world Over 90000 customers have stored their samples.

26. ITERA International Tissue Engineering Research Association CRYSTAL The ITERA Life-Sciences Forum is a consortium in which different European and American universities are participating in fundamental stem cell research. The board of the ITERA Life-Sciences Forum exists of scientists and physicians of different international universities and biotechnological companies. ITERA Life-Sciences Forum is organising an annual international workshop dedicated to the latest developments in stem cell research whereby stem cell researchers are offered a forum to share their findings and ideas. Cryo-Save is a founding member of ITERA and is sponsoring these workshops.

27. Cryo-Save India is situated in Whitefield, Bangalore and this fully automated laboratory which conforms to GLP is placed in an area of 10,000Sqft, this laboratory can store initially 1,50,000 samples which will make it the largest such storage facility in Asia. Cryo-Save India

28. Launch of Cryo-Save India Deccan Chronicle The Hindu Business Line The Times of India Malayala Manorama The Economic Times Dinakaran (Tamil)

29. Cryo save India First Indian company to offer Dual storage More than one time retrieval (cord Blood and Cord Lining) Expansion Possibility Exclusive Logistic services by DHL

30. Why choose Cryo-Save? Storing the cord lining as well at the same cost. The most affordable in the industry. Liabilities/obligations well defined. Audited by the best global auditing firms for compliances. We take the additional responsibility in insuring all samples for the clients safety. The inventories software systems allow easy retrieval. The security and confidentiality of the clients sample and documents are done by a highly integrated access control systems. The largest storage facility in Asia. A GLP conforming laboratory which excells global standards. Cryo-Save has never been, and does not intend to become, involved in the storage or expansion of embryonic stem cells

31. Enrollment and Logistics

32. Registration Process Registration Kit Box Delivery Collection Dispatch Intimation to Doctor Paramedic Doctor

33. Post Registration procedure Sample Received at Lab Sample Processing Maternal Sample Inspection Cryo preservation Final Report to Client

34. Cryo-save India (part of Cryo-Save NV) Payments and refund options 3 PAYMENT OPTIONS Sr No. Plan Ref Plan options First Payment Second Payment Third Payment 1 CS08001 Standard option 1 75,000 2 CS08002 Standard option 2 25,000 20,000 30,000 3 CS08003 Standard Option 3 25,000 11 PDC’s of Rs 5000

35. Our commitments Well before the expected date of delivery: We will issue you with a kit box which you have to carry till the delivery and all procedures are clearly stated. Birth We will be in touch with you to remind you to carry the kit box during your last minute packing. We will speak to your Obstetrician and help him/her in collection if required. Immediately after birth The DHL or your courier can carry the kit box till the laboratory within 48 hours. At the laboratory We will be testing and processing the umbilical cord blood, followed by the cryo-preservation of stem cells . You will receive a telephonic confirmation followed by email on the status of the sample within 48 hours. After 4 weeks You will receive a certificate concerning the storage of the stem cells of your child. You will keep hearing from our client relations executive on the happenings in stem cell industry and the company . After 21 years Before the agreed storage period, we will intimate your (now grown up) child for he may extend the agreement.

36. All the best