Chemical structure Class of natural product Market preparations Isolation of the natural product: Fresh plant from leave or root of citrus Insect-damaged and fungus-infested roots and leaves Were removed Dried in the Laboratory at room temperature until they broke easily by hand Extracted Successively with hexane, chloroform, ethyl acetate and methanol Under vacuum on rotatory evaporator below 50°C Extract was then evaporated and yield a brownish mass A well-stirred suspension of silica gel in Petroleum ether at 60°C–80°C was poured into a column   When the absorbent was well settled, the excess of petrol-ether was allowed to pass through the column. The slurry was passed through the silica gel in petrol-ether and was digested to well stirred column. The column was successively eluted with hexane, chloroform, methanol, and their mixtures of increasing polarity. Yellow powder Pharmacological actions & Mechanism of actions Quantitative Estimation Side effects Contraindications Take care that Naringenin can interact with a quantity of pharmaceutical drugs, changing and influencing their blood level. These include Statins thatare used for cholesterol control antiviral agents used to treat HIV/AIDS anti-anxiety drugs calcium channel blockers that are used to treat high blood pressure non-sedating antihistamines such as Hismanal (astemizole) immunosuppressive drugs used to prevent rejection of transplanted tissues organ.

1. Farah Sabah - 145587
Mario Anwar - 151057
Miray Emad - 170091
Menrva Father Moisis - 170429
Verena Elkes - 155379

2. Chemical structure
C15H12O5

3. Class of natural product
Naringenin is
colourless
and better.
Its class is
flavonoid that
belongs to
the flavanone
family.
they are
mainly found
in tomatoes
and citrus
fruits skin.
It have
potent anti-
oxidant and
anti-
inflammatory
activities.

4. Market preparations

5. Isolation of the natural product
Fresh plant from leave or root of citrus
Insect-damaged and fungus-infested roots and leaves
Were removed
Dried in the Laboratory at room temperature until they broke easily by hand
Extracted Successively with hexane, chloroform, ethyl acetate and methanol
Under vacuum on rotatory
evaporator below 50°C
Extract was then evaporated and yield a brownish mass

6. Isolation of the natural product
A well-stirred suspension of silica gel in Petroleum ether at 60°C–80°C was poured
into a column
• When the absorbent was well settled, the excess of petrol-ether was allowed to
pass through the column. The slurry was passed through the silica gel in petrol-
ether and was digested to well stirred column. The column was successively
eluted with hexane, chloroform, methanol, and their mixtures of increasing
polarity.
Yellow powder

7. Pharmacological actions & Mechanism of actions:
Inhibitory effect
Atherosclerosis
In Diabetes
Heart beneficence
Antioxidant
Inhibition of breast cancer

8. Qualitative Estimation
By HPLC with empower v.2 software for data collection and analysis.
The extract of naringenin is
weighted, mixed with the mobile
phase.
then directly injected into HPLC
system by an amount of 20 µL.
Data were reported as mean of ±
standard deviation.
by comparing the retention time
of the tested compound with the
pure standard compound of
naringenin, identification was
performed

9. Quantitative Estimation
 - NP-HPTLC
 - in NP-HPTLC plates in which 6mm wide and 8mm apart were the
band size of each track.
 - on HPTLC plates, the samples were applied.

10. Quantitative Estimation
 - In pre-saturated twin-through chamber, the plates were developed at room
temperature and 60 ± 5 % humidity using toluene, formic acid and ethyl acetate.
 - Then at 360 and 270 nm absorbance, the dried and developed plates were
quantitatively analyzed.

11. Side effects
Dizziness
Cough
Headache
Palpitation
Flushing sensation
Liver dysfunction

12. Contraindications
• Statins thatare used for cholesterol control antiviral agents used
to treat HIV/AIDS
• anti-anxiety drugs
• calcium channel blockers that are used to treat high blood
pressure
• non-sedating antihistamines such as Hismanal (astemizole)
• immunosuppressive drugs used to prevent rejection of
transplanted tissues organ.
Take care that Naringenin can interact with a
quantity of pharmaceutical drugs, changing and
influencing their blood level. These include

13. References
 Mulvihill, E.E., Assini, J.M., Sutherland, B.G., Dimattia, A., Khami, M., Koppes, J.B., Sawyez, C.G., Whitman, S.C.,
& Huff, M.W. (2010). Naringenin decreases progression of atherosclerosis by improving dyslipidemia in high-
fat-fed low-density lipoprotein receptor-null mice. Arteriosclerosis, thrombosis, and vascular biology, 30 4,
742-8 .
 Kannappan, S. and Anuradha, C.V., 2010. Naringenin enhances insulin-stimulated tyrosine phosphorylation
and improves the cellular actions of insulin in a dietary model of metabolic syndrome. European journal of
nutrition, 49(2), pp.101-109.
 Alam, P. et al. (2017) ‘Quantitative analysis of rutin , quercetin , naringenin , and gallic acid by validated RP-
and NP-HPTLC methods for quality control of anti-HBV active extract of Guiera senegalensis’, Pharmaceutical
Biology. Informa Healthcare USA
 Armelle T. Mbaveng, ... Victor Kuete, in Toxicological Survey of African Medicinal Plants, 2014