The document discusses the comparison between azidoglutamine and azide 1 as glutamine analogues in their ability to bind to cells. Testing on 4T1 RLR, HT1080 luc2, and HepG2 luc2 cell lines showed that azide 1 inhibition of glutamine uptake was greater than azidoglutamine, though not as high as azide 2. This suggests azide 1 binds to cells through a different mechanism than typical glutamine transporters. The effect on the 4T1 RLR line indicates potential involvement of system A or L transporters. Differences in azide 1 binding between cell lines also point to a lesser role for the ASCT2 transporter. All three cell types showed growth