Molecular diagnostics techniques
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Molecular diagnostics is a collection of techniques used to analyse biological markers in the genome and proteome—the individual's genetic code and how their cells express their genes as proteins—by applying molecular biology to medical testing.
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Molecular diagnostic techniques
This document discusses molecular diagnostic techniques used in pathology. It describes common techniques like PCR, blotting methods including Southern blot, Northern blot and Western blot, and hybridization techniques such as in situ hybridization and FISH. These techniques allow manipulation and analysis of DNA, RNA and proteins and have applications in neoplastic disorders, infectious diseases, inherited conditions and identity determination. The document provides details on the principles, requirements and procedures of various molecular diagnostic techniques and their uses in hematological and non-hematological malignancies, infectious diseases, inherited genetic disorders and identity determination.
Molecular techniques
This document discusses various molecular techniques used for diagnosis of infectious diseases. It notes that molecular methods are most useful for pathogens that are difficult to detect by conventional methods, like Mycobacterium tuberculosis and Chlamydia trachomatis. It describes techniques like PCR, NASBA, TBA, SDA, LAMP that amplify nucleic acids from pathogens. Other methods discussed include plasmid profiling, nucleotide sequencing, restriction fragment length polymorphism (RFLP), and nucleic acid hybridization. The document provides details on how several of these techniques work and their applications in microbial identification, detection of antibiotic resistance, and epidemiological studies.
Pcr and its types
The document discusses various types of polymerase chain reaction (PCR) techniques. It begins by explaining what PCR is and how it works to exponentially amplify DNA sequences. It then covers the history of PCR's invention and describes the basic components and steps of a PCR reaction. The document proceeds to discuss different PCR techniques like real-time PCR, asymmetric PCR, colony PCR, and nested PCR. It concludes by noting some applications and limitations of PCR.
Role of pcr in diagnostics
The document discusses the increasing role of PCR in medical diagnostics. It begins by explaining what PCR is and how it works to amplify DNA segments. It then describes the three main uses of PCR in clinical settings: 1) to detect genetic mutations, 2) to detect microbial genes in samples, and 3) to amplify human DNA from limited samples. The rest of the document provides examples of how PCR has improved the diagnosis of genetic diseases and infections compared to previous methods. It concludes that while PCR has limitations, it has proven more sensitive than gold standard tests in many cases by overcoming barriers of other diagnostic techniques.
DNA microarray
Dr. Shamalamma S. presented on DNA microarrays. DNA microarrays allow thousands of genes to be compared simultaneously by attaching DNA probes to a chip which fluorescently labeled samples can bind to. The chip is then scanned to analyze gene expression levels. Applications include disease diagnosis, toxicology studies, and pharmacogenomics. While a powerful tool, microarrays have limitations such as lack of knowledge about many genes and lack of standardization.
Real time PCR
Real time PCR allows for monitoring of DNA amplification during polymerase chain reaction (PCR), rather than just at the end. There are two main detection methods: using non-specific fluorescent dyes that bind to double stranded DNA, and using sequence-specific fluorescent probes. Common non-specific dyes include SYBR Green I, while TaqMan probes are an example of sequence-specific probes that use fluorescence resonance energy transfer. Real time PCR has applications in disease diagnosis, microbiology research on food and water safety, and quantifying gene expression levels.
Molecular Techniques For Disease Diagnosis
Molecular techniques are used to analyze biological markers in genomes and proteomes. They provide several advantages over traditional diagnostic methods such as faster diagnosis, increased sensitivity and specificity, and ability to detect pathogens more rapidly. Common molecular techniques include PCR, real-time PCR, nucleic acid sequencing, microarrays, and nucleic acid amplification methods like NASBA. These techniques are useful for diagnosing infectious diseases and genetic conditions.
Sanger sequencing
The document discusses Sanger sequencing, a method of DNA sequencing. It provides a brief history of DNA sequencing, noting that Sanger developed an enzymatic DNA sequencing technique in 1977. The document then describes the key steps of Sanger sequencing, including separating the DNA strands, copying one strand with chemically altered bases that cause termination, and analyzing the fragments to reveal the DNA sequence. It also compares Sanger sequencing to the Maxam-Gilbert chemical degradation method.
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Molecular diagnostic techniques
This document discusses molecular diagnostic techniques used in pathology. It describes common techniques like PCR, blotting methods including Southern blot, Northern blot and Western blot, and hybridization techniques such as in situ hybridization and FISH. These techniques allow manipulation and analysis of DNA, RNA and proteins and have applications in neoplastic disorders, infectious diseases, inherited conditions and identity determination. The document provides details on the principles, requirements and procedures of various molecular diagnostic techniques and their uses in hematological and non-hematological malignancies, infectious diseases, inherited genetic disorders and identity determination.
Molecular techniques
This document discusses various molecular techniques used for diagnosis of infectious diseases. It notes that molecular methods are most useful for pathogens that are difficult to detect by conventional methods, like Mycobacterium tuberculosis and Chlamydia trachomatis. It describes techniques like PCR, NASBA, TBA, SDA, LAMP that amplify nucleic acids from pathogens. Other methods discussed include plasmid profiling, nucleotide sequencing, restriction fragment length polymorphism (RFLP), and nucleic acid hybridization. The document provides details on how several of these techniques work and their applications in microbial identification, detection of antibiotic resistance, and epidemiological studies.
Pcr and its types
The document discusses various types of polymerase chain reaction (PCR) techniques. It begins by explaining what PCR is and how it works to exponentially amplify DNA sequences. It then covers the history of PCR's invention and describes the basic components and steps of a PCR reaction. The document proceeds to discuss different PCR techniques like real-time PCR, asymmetric PCR, colony PCR, and nested PCR. It concludes by noting some applications and limitations of PCR.
Role of pcr in diagnostics
The document discusses the increasing role of PCR in medical diagnostics. It begins by explaining what PCR is and how it works to amplify DNA segments. It then describes the three main uses of PCR in clinical settings: 1) to detect genetic mutations, 2) to detect microbial genes in samples, and 3) to amplify human DNA from limited samples. The rest of the document provides examples of how PCR has improved the diagnosis of genetic diseases and infections compared to previous methods. It concludes that while PCR has limitations, it has proven more sensitive than gold standard tests in many cases by overcoming barriers of other diagnostic techniques.
DNA microarray
Dr. Shamalamma S. presented on DNA microarrays. DNA microarrays allow thousands of genes to be compared simultaneously by attaching DNA probes to a chip which fluorescently labeled samples can bind to. The chip is then scanned to analyze gene expression levels. Applications include disease diagnosis, toxicology studies, and pharmacogenomics. While a powerful tool, microarrays have limitations such as lack of knowledge about many genes and lack of standardization.
Real time PCR
Real time PCR allows for monitoring of DNA amplification during polymerase chain reaction (PCR), rather than just at the end. There are two main detection methods: using non-specific fluorescent dyes that bind to double stranded DNA, and using sequence-specific fluorescent probes. Common non-specific dyes include SYBR Green I, while TaqMan probes are an example of sequence-specific probes that use fluorescence resonance energy transfer. Real time PCR has applications in disease diagnosis, microbiology research on food and water safety, and quantifying gene expression levels.
Molecular Techniques For Disease Diagnosis
Molecular techniques are used to analyze biological markers in genomes and proteomes. They provide several advantages over traditional diagnostic methods such as faster diagnosis, increased sensitivity and specificity, and ability to detect pathogens more rapidly. Common molecular techniques include PCR, real-time PCR, nucleic acid sequencing, microarrays, and nucleic acid amplification methods like NASBA. These techniques are useful for diagnosing infectious diseases and genetic conditions.
Sanger sequencing
The document discusses Sanger sequencing, a method of DNA sequencing. It provides a brief history of DNA sequencing, noting that Sanger developed an enzymatic DNA sequencing technique in 1977. The document then describes the key steps of Sanger sequencing, including separating the DNA strands, copying one strand with chemically altered bases that cause termination, and analyzing the fragments to reveal the DNA sequence. It also compares Sanger sequencing to the Maxam-Gilbert chemical degradation method.
DNA Sequencing
This document discusses the history and various methods of DNA sequencing. It begins with a brief overview of DNA sequencing and its uses. It then outlines some of the major developments in DNA sequencing techniques, including the earliest RNA sequencing in 1972, Sanger sequencing in 1977, and the first complete genome of Haemophilus influenzae in 1995. The document proceeds to provide more detailed explanations of several DNA sequencing methods, such as Sanger sequencing, pyrosequencing, shotgun sequencing, Illumina sequencing, and SOLiD sequencing.
Molecular diagnostics
The document discusses molecular diagnostics and genetic testing techniques. It provides an overview of molecular diagnostics, their significance in medicine, and how they are transforming fields like prenatal testing, disease detection, and drug selection. It then covers various immunological diagnostic methods like ELISA, radioimmunoassay, western blotting, and their characteristics. The document also discusses molecular genetic tests, genetic alterations detected, and techniques for DNA-based diagnosis of diseases. It focuses on the principles and procedures of molecular diagnostic methods like hybridization assays and PCR and their applications in detecting pathogens and genetic disorders.
shotgun sequncing
Genome sequencing is the process of determining the order of nucleotide bases - A, C, G, and T - that make up an organism's DNA. Shotgun sequencing involves randomly breaking the genome into small fragments, sequencing those pieces, and reassembling the sequence by identifying overlapping regions. It was originally used by Sanger to sequence small genomes like viruses and bacteria. There are two main methods - hierarchical shotgun sequencing for larger genomes containing repeats, and whole genome shotgun sequencing for smaller genomes.
Dna microarray (dna chips)
This document provides an overview of DNA microarrays, also known as DNA chips. It discusses the principles and techniques used to prepare DNA microarrays, including photolithography. There are two main types of DNA chips: cDNA-based chips and oligonucleotide-based chips. DNA microarrays have various applications, including gene expression profiling, drug discovery, and diagnostics. They provide the advantage of analyzing thousands of genes simultaneously but also have disadvantages such as high costs and complex data analysis.
Real time pcr
Real-time PCR allows for the continuous collection of fluorescent data during the PCR process, allowing for quantification of the amount of PCR product accumulated in each cycle. It provides advantages over conventional PCR such as increased precision, sensitivity, and automation. Various chemistries can be used including SYBR Green, TaqMan probes, molecular beacons, and scorpion primers, which rely on fluorescent dyes and quenchers. Real-time PCR finds applications in gene expression analysis, pathogen detection, and DNA damage measurement by allowing quantitative analysis.
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DNA footprinting is a technique used to identify where proteins bind to DNA. It was developed in 1978 and works by treating DNA with enzymes or chemicals that cut DNA, except for regions bound by proteins. This leaves a "footprint" where the protein is bound and protects the DNA. There are two main types: DNase I footprinting cuts DNA randomly except where proteins are bound, while DMS footprinting modifies DNA bases except where proteins protect them from modification. The cut or modified DNA is then run on a gel to identify the protein binding site. DNA footprinting is useful for mapping transcription factor binding sites and studying protein-DNA interactions.
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elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
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1. A DNA microarray contains thousands of DNA probes attached to a solid surface in defined locations. Each probe represents a single gene.
2. Sample mRNA is converted to fluorescently labeled cDNA and hybridized to the DNA microarray. The level of fluorescence indicates the expression level of each gene.
3. After washing, the microarray is scanned and analyzed to determine changes in gene expression between control and test samples. This allows high-throughput analysis of gene expression profiles.
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DNA microarray
DNA microarrays allow analysis of gene expression across thousands of genes simultaneously. They consist of DNA probes attached to a solid surface in an organized grid pattern, with each spot representing a single gene. Samples are labeled with fluorescent dyes and hybridized to the chip. Complementary sequences pair via hydrogen bonds, while non-specific sequences are washed away. The signal intensity at each spot indicates the amount of target sequence present and thus gene expression levels. DNA microarrays have applications in clinical diagnosis, drug discovery, and other fields by profiling gene expression patterns.
Nucleic acid hybridization
Nucleic acid hybridization is a technique where single-stranded nucleic acid molecules form double-stranded molecules through hydrogen bonding between complementary base sequences. This process can identify specific DNA or RNA sequences through the use of labeled probes. There are different types of hybridization including Southern blot, which uses probes to detect complementary DNA sequences separated by electrophoresis; Northern blot, which detects RNA sequences; and colony hybridization, which isolates plasmids containing a particular sequence.
Types of PCR
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Gene cloning strategies
Gene cloning strategies depend on whether genomic or cDNA libraries are being constructed. Shotgun cloning is used to construct genomic libraries by fragmenting genomic DNA and inserting all fragments into vectors at once. cDNA libraries are constructed by reverse transcribing mRNA to cDNA, which is then cloned into vectors. Both library types are screened to identify overlapping clones that are assembled into contigs representing the entire genome.
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Blooting technique1
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Genome sequencing is the process of determining the order of nucleotide bases - A, C, G, and T - that make up an organism's DNA. Shotgun sequencing involves randomly breaking the genome into small fragments, sequencing those pieces, and reassembling the sequence by identifying overlapping regions. It was originally used by Sanger to sequence small genomes like viruses and bacteria. There are two main methods - hierarchical shotgun sequencing for larger genomes containing repeats, and whole genome shotgun sequencing for smaller genomes.
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This document provides an overview of DNA microarrays, also known as DNA chips. It discusses the principles and techniques used to prepare DNA microarrays, including photolithography. There are two main types of DNA chips: cDNA-based chips and oligonucleotide-based chips. DNA microarrays have various applications, including gene expression profiling, drug discovery, and diagnostics. They provide the advantage of analyzing thousands of genes simultaneously but also have disadvantages such as high costs and complex data analysis.
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Real-time PCR allows for the continuous collection of fluorescent data during the PCR process, allowing for quantification of the amount of PCR product accumulated in each cycle. It provides advantages over conventional PCR such as increased precision, sensitivity, and automation. Various chemistries can be used including SYBR Green, TaqMan probes, molecular beacons, and scorpion primers, which rely on fluorescent dyes and quenchers. Real-time PCR finds applications in gene expression analysis, pathogen detection, and DNA damage measurement by allowing quantitative analysis.
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DNA footprinting is a technique used to identify where proteins bind to DNA. It was developed in 1978 and works by treating DNA with enzymes or chemicals that cut DNA, except for regions bound by proteins. This leaves a "footprint" where the protein is bound and protects the DNA. There are two main types: DNase I footprinting cuts DNA randomly except where proteins are bound, while DMS footprinting modifies DNA bases except where proteins protect them from modification. The cut or modified DNA is then run on a gel to identify the protein binding site. DNA footprinting is useful for mapping transcription factor binding sites and studying protein-DNA interactions.
NEXT GENERATION SEQUENCING
Deciphering DNA sequences is essential for virtually all branches of biological research. With the
advent of capillary electrophoresis (CE)-based Sanger sequencing, scientists gained the ability to
elucidate genetic information from any given biological system. This technology has become widely
adopted in laboratories around the world, yet has always been hampered by inherent limitations in
throughput, scalability, speed, and resolution that often preclude scientists from obtaining the essential
information they need for their course of study. To overcome these barriers, an entirely new technology
was required—Next-Generation Sequencing (NGS), a fundamentally different approach to sequencing
that triggered numerous ground-breaking discoveries and ignited a revolution in genomic science.
DNA microarray final ppt.
1. A DNA microarray contains thousands of DNA probes attached to a solid surface in defined locations. Each probe represents a single gene.
2. Sample mRNA is converted to fluorescently labeled cDNA and hybridized to the DNA microarray. The level of fluorescence indicates the expression level of each gene.
3. After washing, the microarray is scanned and analyzed to determine changes in gene expression between control and test samples. This allows high-throughput analysis of gene expression profiles.
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Reverse transcriptase polymerase chain reaction
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This document provides information on Ouchterlony double diffusion and radial immunodiffusion techniques. Ouchterlony double diffusion involves placing antigen and antibody in wells in an agar gel plate and observing the interaction over 24-48 hours. Possible results are identity, non-identity, or partial identity based on fusion of lines. Radial immunodiffusion is similar but incorporates antibody into the gel and antigens diffuse outward, forming measurable circular precipitin rings. Both techniques are used to detect and quantify the presence of antigens and antibodies.
DNA microarray
DNA microarrays allow analysis of gene expression across thousands of genes simultaneously. They consist of DNA probes attached to a solid surface in an organized grid pattern, with each spot representing a single gene. Samples are labeled with fluorescent dyes and hybridized to the chip. Complementary sequences pair via hydrogen bonds, while non-specific sequences are washed away. The signal intensity at each spot indicates the amount of target sequence present and thus gene expression levels. DNA microarrays have applications in clinical diagnosis, drug discovery, and other fields by profiling gene expression patterns.
Nucleic acid hybridization
Nucleic acid hybridization is a technique where single-stranded nucleic acid molecules form double-stranded molecules through hydrogen bonding between complementary base sequences. This process can identify specific DNA or RNA sequences through the use of labeled probes. There are different types of hybridization including Southern blot, which uses probes to detect complementary DNA sequences separated by electrophoresis; Northern blot, which detects RNA sequences; and colony hybridization, which isolates plasmids containing a particular sequence.
Types of PCR
This document discusses different types of polymerase chain reaction (PCR) techniques. It begins by providing background on PCR and its development. It then describes several types of PCR including multiplex PCR, which allows for simultaneous detection of multiple pathogens; nested PCR, which increases specificity; reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR), which are used to detect RNA; quantitative PCR, which measures specific target DNA/RNA amounts; and other variants like hot-start PCR, touchdown PCR, and methylation-specific PCR. Each type is briefly explained along with its uses and applications in medical research.
Gene cloning strategies
Gene cloning strategies depend on whether genomic or cDNA libraries are being constructed. Shotgun cloning is used to construct genomic libraries by fragmenting genomic DNA and inserting all fragments into vectors at once. cDNA libraries are constructed by reverse transcribing mRNA to cDNA, which is then cloned into vectors. Both library types are screened to identify overlapping clones that are assembled into contigs representing the entire genome.
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Ligase Chain Reaction(LCR)
The document summarizes the ligase chain reaction (LCR) technique. LCR amplifies DNA sequences using four probes and ligase and polymerase enzymes instead of amplifying DNA through nucleotide polymerization like PCR. It can detect single nucleotide polymorphisms. The process involves denaturing the DNA, annealing probes to the target sequence, and ligating the probes if they match, repeating for exponential amplification. Products are detected through gel electrophoresis or non-radioactive methods. LCR has applications in infectious disease detection, genotyping and mutation analysis.
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Presentation blotting
The document discusses various molecular techniques including blotting, probes, and polymerase chain reaction (PCR). It describes Southern blotting for detecting DNA, Northern blotting for RNA, and Western blotting for proteins. It explains how probes are used to identify specific DNA or RNA sequences in Southern and Northern blotting. The key steps of PCR are outlined, including denaturation, annealing of primers, and extension of DNA copies. Applications of these techniques include gene discovery, mutation detection, forensics, diagnosis of genetic disorders, and more. PCR has revolutionized research and diagnostics due to its speed, sensitivity, and specificity.
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Presentation blotting techniques
Paramjeet Singh presented on various blotting techniques used to detect specific DNA, RNA, and proteins. Southern blotting is used to detect DNA sequences and was developed by Edwin Southern in 1975. It involves separating DNA fragments via gel electrophoresis, transferring them to a membrane, and using probes to detect the target sequence. Northern blotting detects RNA and was modeled after Southern blotting. Western blotting, or immunoblotting, detects specific proteins and uses gel electrophoresis, transfer to a membrane, and antibodies to identify proteins. These techniques were crucial to molecular biology research but have been largely replaced by more sensitive methods like PCR and ELISA.
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Genomic DNA and cDNA can be used to produce target DNA for cloning. Genomic DNA contains introns and other noncoding sequences, while cDNA is produced from mRNA and does not contain introns. Probes, which are DNA or RNA fragments labeled with radioactive or fluorescent tags, can be used to detect complementary nucleotide sequences in DNA or RNA samples. Southern blotting is a technique used to detect specific DNA fragments and involves cleavage of DNA, electrophoresis, transfer to a membrane, and detection using a labeled probe. Factors limiting the effectiveness of gene therapy include problems integrating DNA into the genome long-term and immune responses against foreign DNA.
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Microarray
If a microbiologist is studying bacteria that premeditate, or break down, toxic wastes and wants to know which specific genes are active when that bacterium is degrading, say, PCBs, he would likely use a tool called the DNA microarray.
Microarrays enable scientists to monitor the activities of hundreds or thousands of genes at once. All microarrays (also called DNA chips or gene chips) work on the basic principle that complementary nucleotide sequences in DNA (and RNA) match up like the two halves of a piece of Velcro coming together.
Pattern of gene activity on a microarray chip.
A microarray consists of an orderly arrangement of bits of genetic material in super-tiny spots laid down in a grid on a suitable surface, often a glass slide with a specially chemically treated surface.
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Seminar on dna based diagnosis of genetic diasease
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8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
如何办理(uvic毕业证书)维多利亚大学毕业证本科学位证书原版一模一样
原版纸张【微信：741003700 】【(uvic毕业证书)维多利亚大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Phenomics assisted breeding in crop improvement
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
bordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero WaterTexas Alliance of Groundwater Districts
Presented at June 6-7 Texas Alliance of Groundwater Districts Business MeetingShallowest Oil Discovery of Turkiye.pptx
The Petroleum System of the Çukurova Field - the Shallowest Oil Discovery of Türkiye, Adana
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...Abdul Wali Khan University Mardan,kP,Pakistan
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skillsImmersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
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三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
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◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Recently uploaded (20)
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
aziz sancar nobel prize winner: from mardin to nobel
aziz sancar nobel prize winner: from mardin to nobel
Basics of crystallography, crystal systems, classes and different forms
Basics of crystallography, crystal systems, classes and different forms
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
bordetella pertussis.................................ppt
bordetella pertussis.................................ppt
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
THEMATIC APPERCEPTION TEST(TAT) cognitive abilities, creativity, and critic...
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless Reproducibility
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
Molecular diagnostics techniques
- 2. Presented by: SAYED MUHAMMAD TOUSEEF Department of Biotechnology 2
- 4. Introduction of MOLECULAR DIAGNOSTICS TECHNIQUES Molecular diagnostics is a collection of techniques. used to analyze biological markers in the genome and proteome. The technique is used to diagnose and monitor disease, detect risk, and decide which therapies will work best for individual patients. molecular diagnostics offers the prospect of personalized medicine. 4
- 5. 5
- 6. History In 1980, Yuet Wai Kan et al. suggested a prenatal genetic test for Thalassemia that did not rely upon DNA sequencing—then in its infancy—but on restriction enzymes that cut DNA where they recognized specific short sequences, creating different lengths of DNA strand depending on which allele (genetic variation) the fetus possessed. in 1995, the Association for Molecular Pathology (AMP) was formed to give it structure. 6
- 7. In 1999, the AMP co-founded The Journal of Medical Diagnostics. In 2012, molecular diagnostic techniques for Thalassemia use genetic hybridization tests to identify the specific single nucleotide polymorphism causing an individual's disease 7
- 8. How it works?? Every organisms contain some unique species specific DNA sequences. Molecular diagnostics makes the specie specific DNA visible 8
- 9. Applications Classification of organism based on genetic relatedness (genotyping) Identification and confirmation of isolate obtained from culture Early detection of pathogens in clinical specimen Rapid detection of antibiotic resistance Detection of mutations Detection of toxigenic from non toxigenic strains Also useful in forensic medicine 9
- 10. Techniques used Plasmid profiling Nucleotide sequencing Restriction Fragment Length Polymorphism (RFLP) Amplification techniques 10
- 11. Plasmid profiling Plasmids are the extra chromosomal circular double stranded DNA found in most bacteria Each bacterium has one or several plasmids Cells are lysed and the nucleic acid are subjected to electrophoresis The size and number of plasmids can be estimated Drawback: some species may contain variable number of plasmids or even unrelated bacteria may have similar number of plasmids 11
- 12. 12
- 13. Nucleotide sequencing For determination of the nucleotide sequence in the given DNA molecule Methods: 1. Chemical cleavage method 2 .Chain termination method Not much role in diagnostic microbiology For structure of gene, mutations and to design primers 13
- 14. Restriction Fragment Length Polymorphism (RFLP) Polymorphism in nucleotide sequence is present in all organism Restriction sites are the strands of DNA that are specifically recognized and cleaved by restriction endonucleases Useful as a 1. Epidemiological typing tool 2. Ribotyping – phylogenetic classification 14
- 15. 15
- 16. Nucleic Acid Sequence Based Amplification • Isothermic non PCR procedure • Definition: A primer dependent technology that can be used for the continuous amplification of nucleic acid in a single mixture at one temperature. • 3 SR: self sustained replication • 3 enzymes: AMV reverse transcriptase, Ribonuclease H, t7 RNA polymerase 16
- 17. Transcription Based Amplification • Useful in the amplification of ss RNA rather than DNA • Developed by Gen-probe • Used in clinical laboratories to detect Chlamydia trachomatis and Neisseria gonorrhea 17
- 18. Strand Displacement Amplification • It is an isothermal technique • Based on restriction endonuclease nicking its recognition site and a polymer extending the nick at its 3’ end displacing the downstream strand • Required restriction enzyme cleavage of the DNA sample prior to amplification 18
- 19. cont… • Normally restriction enzyme cleavage produce dsDNA which is not suitable template for SDA • By incorporating alpha thio substituted nucleotides a double stranded hemiphosphorothioated DNA is created where the restriction site in newly synthesized strand is resistant to cleavage 19
- 20. 20
- 21. Signal Amplification • Amplify the signal generated by the labelled probes • bDNA-Branched DNA probes • Hybrid Capture- Anti DNA-RNA hybrid antibody 21
- 22. Cont… • Signal amplification-used to increase the sensitivity of the probe based assays • 103-105 nucleic acid targets can be detected • Branched DNA probe system: • Target sequence is captured using a capture step • Hybridization with an unlabeled probe that has two hybridization sequence • One directed against target sequence • Second hybridizes with bDNA amplification number 22
- 23. Cont… • Multimer system—chemically synthesized oligonucleotide chain with a comb like backbone that can bind to several reporter probes • Highly sensitive because the target nucleic acid has to bind both to the capture as well as target probes before the signals are amplified 23
- 24. THANK-YOU 24