The document discusses the synthesis of nanoparticles using microorganisms such as bacteria and fungi. It describes intracellular and extracellular synthesis methods. Intracellular synthesis involves accumulation of nanoparticles inside the cell, while extracellular synthesis uses cell secretions outside the cell. Specific examples provided include gold and silver nanoparticles synthesized using bacteria and fungi through reduction of metal ions. The nanoparticles have a variety of shapes and sizes in the 1-100 nm range and potential applications.
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The document summarizes research on the biological synthesis of silver nanoparticles using Klebsiella pneumonia bacteria. Silver nanoparticles were successfully synthesized using the cell-free supernatant of K. pneumonia when added to silver nitrate solutions. The nanoparticles were characterized using UV-VIS spectroscopy, SEM, and FTIR, and were found to be spherical in shape with sizes around 21nm. Antimicrobial testing showed the biologically synthesized silver nanoparticles were effective against both gram-positive and gram-negative bacteria. Potential applications of the silver nanoparticles include use in wound dressings and antimicrobial fabrics.
Biological method for the preparation of nanoparticles(Sheersho)Sheersha Pramanik 🇮🇳
This document discusses various biological methods for synthesizing nanoparticles, including using bacteria, fungi, yeast, plants, and waste materials. It describes how nanoparticles can be synthesized intracellularly or extracellularly by bacteria. Specific bacteria used to synthesize silver, gold, iron and other nanoparticles are mentioned. The document also discusses nanoparticle synthesis using fungi, yeasts, plant extracts, and industrial waste. It concludes by noting the promising potential but current limitations of biological nanoparticle synthesis for medical applications.
This document discusses the synthesis and characterization of nanoparticles from biological sources. It describes how bacteria, enzymes, microorganisms, fungi, yeast, and plants can be used to synthesize nanoparticles through extracellular or intracellular processes. The biosynthesis of nanoparticles involves the reduction of metal ions and results in a green chemistry approach. Characterization techniques mentioned include SEM, TEM, XRD, FT-IR, DLS, TGA and zeta potential analysis which are used to determine the nanoparticles' size, shape, composition and properties.
Antimicrobial activity of silver nanoparticles from capsicum sp. against stap...Alexander Decker
This document summarizes a study that synthesized silver nanoparticles from Capsicum sp. (pepper) extract and tested their antimicrobial activity against various pathogens. Key findings:
- Pepper extract was used to reduce silver ions and synthesize silver nanoparticles, confirmed by a UV-Vis absorption peak at 480nm.
- Disk diffusion tests showed the silver nanoparticles had concentration-dependent antibacterial activity against Staphylococcus, Bacillus, E. coli, and Pseudomonas. Higher concentrations inhibited more growth.
- The nanoparticles were most effective against Pseudomonas, followed by Bacillus, Staphylococcus, and E. coli, showing activity against both gram-positive and gram-negative bacteria.
Synthesis of Silver Nano Particles from Marine Bacteria Pseudomonas aerogenosaKamalpreet Sarna
This document summarizes a study that isolated a marine bacterial strain called Pseudomonas aeruginosa and used it to synthesize silver nanoparticles. The silver nanoparticles were characterized using UV-Vis spectroscopy, SEM, FTIR, and XRD. UV-Vis analysis showed a peak at 420nm indicating the presence of silver nanoparticles. SEM images showed the nanoparticles were spherical in shape with sizes ranging from 50-80nm. FTIR and XRD further confirmed the presence of silver. The silver nanoparticles showed potent antibacterial activity against both gram-positive and gram-negative bacteria as well as antifungal activity. This study demonstrates the potential of using marine bacteria as a green synthesis method for producing silver nanoparticles with biological applications.
Bacteria are able to synthesize nanoparticles through intracellular and extracellular processes. Intracellular synthesis occurs inside the cell and can produce nanoparticles of gold, silver, and other metals between 5-25nm in size. Extracellular synthesis happens outside the cell through processes like biomineralization that change pH and produce particles in a variety of shapes and sizes ranging from 28.2nm to 122nm on average. Bacteria provide a green method for nanoparticle synthesis compared to chemical and physical techniques. Common bacteria used include Bacillus cereus and Pseudomonas that have developed defenses against nanoparticle toxicity.
The document discusses the synthesis of nanoparticles using microorganisms such as bacteria and fungi. It describes intracellular and extracellular synthesis methods. Intracellular synthesis involves accumulation of nanoparticles inside the cell, while extracellular synthesis uses cell secretions outside the cell. Specific examples provided include gold and silver nanoparticles synthesized using bacteria and fungi through reduction of metal ions. The nanoparticles have a variety of shapes and sizes in the 1-100 nm range and potential applications.
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The document summarizes research on the biological synthesis of silver nanoparticles using Klebsiella pneumonia bacteria. Silver nanoparticles were successfully synthesized using the cell-free supernatant of K. pneumonia when added to silver nitrate solutions. The nanoparticles were characterized using UV-VIS spectroscopy, SEM, and FTIR, and were found to be spherical in shape with sizes around 21nm. Antimicrobial testing showed the biologically synthesized silver nanoparticles were effective against both gram-positive and gram-negative bacteria. Potential applications of the silver nanoparticles include use in wound dressings and antimicrobial fabrics.
Biological method for the preparation of nanoparticles(Sheersho)Sheersha Pramanik 🇮🇳
This document discusses various biological methods for synthesizing nanoparticles, including using bacteria, fungi, yeast, plants, and waste materials. It describes how nanoparticles can be synthesized intracellularly or extracellularly by bacteria. Specific bacteria used to synthesize silver, gold, iron and other nanoparticles are mentioned. The document also discusses nanoparticle synthesis using fungi, yeasts, plant extracts, and industrial waste. It concludes by noting the promising potential but current limitations of biological nanoparticle synthesis for medical applications.
This document discusses the synthesis and characterization of nanoparticles from biological sources. It describes how bacteria, enzymes, microorganisms, fungi, yeast, and plants can be used to synthesize nanoparticles through extracellular or intracellular processes. The biosynthesis of nanoparticles involves the reduction of metal ions and results in a green chemistry approach. Characterization techniques mentioned include SEM, TEM, XRD, FT-IR, DLS, TGA and zeta potential analysis which are used to determine the nanoparticles' size, shape, composition and properties.
Antimicrobial activity of silver nanoparticles from capsicum sp. against stap...Alexander Decker
This document summarizes a study that synthesized silver nanoparticles from Capsicum sp. (pepper) extract and tested their antimicrobial activity against various pathogens. Key findings:
- Pepper extract was used to reduce silver ions and synthesize silver nanoparticles, confirmed by a UV-Vis absorption peak at 480nm.
- Disk diffusion tests showed the silver nanoparticles had concentration-dependent antibacterial activity against Staphylococcus, Bacillus, E. coli, and Pseudomonas. Higher concentrations inhibited more growth.
- The nanoparticles were most effective against Pseudomonas, followed by Bacillus, Staphylococcus, and E. coli, showing activity against both gram-positive and gram-negative bacteria.
Synthesis of Silver Nano Particles from Marine Bacteria Pseudomonas aerogenosaKamalpreet Sarna
This document summarizes a study that isolated a marine bacterial strain called Pseudomonas aeruginosa and used it to synthesize silver nanoparticles. The silver nanoparticles were characterized using UV-Vis spectroscopy, SEM, FTIR, and XRD. UV-Vis analysis showed a peak at 420nm indicating the presence of silver nanoparticles. SEM images showed the nanoparticles were spherical in shape with sizes ranging from 50-80nm. FTIR and XRD further confirmed the presence of silver. The silver nanoparticles showed potent antibacterial activity against both gram-positive and gram-negative bacteria as well as antifungal activity. This study demonstrates the potential of using marine bacteria as a green synthesis method for producing silver nanoparticles with biological applications.
Bacteria are able to synthesize nanoparticles through intracellular and extracellular processes. Intracellular synthesis occurs inside the cell and can produce nanoparticles of gold, silver, and other metals between 5-25nm in size. Extracellular synthesis happens outside the cell through processes like biomineralization that change pH and produce particles in a variety of shapes and sizes ranging from 28.2nm to 122nm on average. Bacteria provide a green method for nanoparticle synthesis compared to chemical and physical techniques. Common bacteria used include Bacillus cereus and Pseudomonas that have developed defenses against nanoparticle toxicity.
Abstract
A rapid advance of nanotechnology has the potential approach for significant improvements in disease prevention, diagnosis and treatment. In this article, we report a simple and eco-friendly biosynthesis of silver nanoparticles (Ag-NPs) using silver nitrate as metal precursor in Curcuma longa. These Ag-NPs were characterized by UV–vis spectroscopy, and Transmission electron microscopy (TEM). These nanoparticles exhibited maximum absorbance in specific nano meter range in UV–vis spectroscopy. TEM micrographs revealed the formation of well-dispersed Ag-NPs with its size and morphology. Microbiology assay founds that Ag-NPs are effective against V.cholera bacteria. These developments raise exciting opportunities to diagnose and treat pathogenic mode of infection based on the various profiles to target diseases.
Synthesis of the silver nanoparticles from Aloe barbadensis extract and its a...IRJET Journal
This document summarizes a study that synthesized silver nanoparticles from Aloe barbadensis leaf extract and evaluated their antimicrobial activity against urinary tract infection pathogens. Key findings include:
- Silver nanoparticles were successfully synthesized using Aloe barbadensis leaf extract and characterized using UV-Vis spectroscopy and FTIR.
- Thirty-two bacteria isolated from urine samples were identified, with Escherichia coli being the most prevalent pathogen identified in 47% of samples.
- Disc diffusion assays found the silver nanoparticles showed strongest antimicrobial activity against E. coli, followed by Candida albicans. Klebsiella pneumoniae and Staphylococcus aureus showed no sensitivity.
- Results indicate silver
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The document summarizes research characterizing gold nanoparticles synthesized intracellularly by the biomass of Aspergillus terreus fungus. When an aqueous solution of chloroauric acid was reduced by A. terreus biomass, it changed color from yellow to pinkish violet, indicating gold nanoparticle formation. The synthesized nanoparticles were spherical or irregularly shaped with an average size of 186 nm. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction were used to characterize the nanoparticles and confirmed the presence of crystalline gold nanoparticles capped by biomolecules from the fungus.
This document discusses the topic of nanobioremediation. It begins with introductions to nanoscience and bioremediation. Nanobioremediation uses nanoscience and nanoparticles to enhance microbial activity and remove pollutants more quickly. Examples are given of using genetic engineering and nanoparticles to modify microbes for improved bioremediation. The document concludes that nanobioremediation can clean environmental hazards faster and safer than other methods while meeting economic, social, and environmental criteria.
Biosynthesis of Silver Nanoparticles (AgNPs) and it's ApplicationsManish Dash
The document discusses the green synthesis and applications of silver nanoparticles. It begins by outlining the need for advanced disinfectant nanomaterials to prevent disease outbreaks caused by overpopulation and poor sanitation. It then describes how silver nanoparticles are a promising material for developing antimicrobial products due to their high antimicrobial activity. The document goes on to detail a green synthesis method for producing silver nanoparticles using the extract of Bougainvillea plant bracts, and characterizes the nanoparticles. It finds that the synthesized silver nanoparticles demonstrate effective antimicrobial properties against bacteria as well as antifouling effects.
The document summarizes a study that biologically synthesized and characterized intracellular gold nanoparticles using the biomass of Aspergillus fumigatus. A. fumigatus was grown in liquid culture and its biomass was then exposed to chloroauric acid solution, resulting in the intracellular production of gold nanoparticles within 72 hours, as indicated by a color change. The synthesized nanoparticles were characterized through various techniques and were found to be spherical and irregularly shaped, ranging from 85.1 to 210 nm in size. Scanning electron microscopy revealed the nanoparticles accumulated on the fungal mycelia. Energy dispersive spectroscopy and X-ray diffraction confirmed the presence and crystalline nature of the synthesized gold nanoparticles.
This document summarizes a study that used the leaves of lemongrass (Cymbopogan citratus) to rapidly synthesize silver nanoparticles. Silver nanoparticles formed within 8-10 minutes when a lemongrass leaf extract reacted with silver nitrate under microwave irradiation. The nanoparticles were characterized using UV-visible spectroscopy, nanoparticle tracking analysis, transmission electron microscopy, and energy dispersive X-ray spectroscopy. The silver nanoparticles showed antimicrobial activity against bacteria such as E. coli and S. aureus as well as fungi such as C. albicans, inhibiting their growth in laboratory assays. When combined with antibiotics, the silver nanoparticles enhanced the antibiotics' effectiveness against drug-resistant microbial strains.
This study evaluated the biosorption of zinc and nickel from wastewater using dead fungal biomass of Aspergillus flavus. The maximum biosorption capacity for zinc was found to be 47.36% at pH 6.5, with a biomass concentration of 2g/L, contact time of 50 minutes, and zinc concentration of 2ppm. For nickel, the maximum biosorption capacity was 61.60% at pH 5, with a biomass concentration of 2g/L, contact time of 60 minutes, and nickel concentration of 2ppm. Desorption using 0.1M HCl was found to be effective in removing zinc and nickel from the fungal biomass,
Nanotechnology in cancer and its synthesisShreyaBhatt23
basic introduction to nanotechnology and the types of nanomaterials used in medical purpose. sysnthesis of nanomaterials by physical , chemical, biosynthesis, green synthesis of nanomaterials
Bio synthesis of nano particles using bacteriaudhay roopavath
Bacteria can be used to biosynthesize nanoparticles through intracellular and extracellular methods. Intracellular synthesis occurs inside the cell, where bacteria reduce metal ions and deposit nanoparticles in locations like the periplasmic space. Extracellular synthesis involves enzymes secreted by bacteria reducing metal ions outside the cell and precipitating nanoparticles. Examples are given of bacteria producing silver, titanium, zinc sulfide and lead sulfide nanoparticles through extracellular and intracellular pathways. While a green approach, bacterial nanoparticle synthesis can be slow with difficulty controlling size, shape and crystallinity of particles.
Role of salt precursor in the synthesis of zinc oxide nanoparticleseSAT Journals
Abstract In this paper, Zinc oxide nanoparticles having wurtzite crystalline structure are synthesized. The temperature, base concentration and the salt precursor used for the synthesis affects the morphology and particle size. The synthesized nanoparticles are characterized by X-ray diffraction, Scanning electron microscopy and Diffused reflectance UV-visible spectroscopy. As the temperature is increased from 800 C to 1000C, keeping the concentration of the base viz sodium hydroxide at 5M, the particle size increases from 30nm to 500nm. With change in base concentration from 2M to 10M, at constant reaction temperature of 800C, the particle size increases from 30 nm to 500 nm. Herein the precursor used is zinc chloride. The effect of salt precursor is studied for three different salt precursors, viz. zinc chloride, zinc nitrate and zinc acetate. This paper is an attempt to give the information about salt precursor to be used, optimum values of temperature and base concentration for synthesis of the ZnO nanoparticles with enhanced antibacterial property for suitable biomedical application. Keywords: Zinc Oxide nanoparticles, metal oxide nanoparticles, nanotechnology.
Role of salt precursor in the synthesis of zinc oxide nanoparticleseSAT Publishing House
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
The document summarizes the biosynthesis of copper nanoparticles using Beta Vulgaris extract and their antibacterial properties. Key points:
1) Beta Vulgaris extract was used to biosynthesize copper nanoparticles due to its abundance of stabilizing compounds like betalains.
2) Characterization techniques like UV-Vis, SEM, EDAX, and FTIR confirmed the formation of mono-dispersed copper nanoparticles around 35-60 nm in size.
3) Disc diffusion tests showed the biologically synthesized copper nanoparticles had good antibacterial activity against various bacterial strains like E. coli, with the greatest inhibition of E. coli growth.
Antimicrobial and cytotoxicity effect of silver nanoparticle synthesized by C...Nanomedicine Journal (NMJ)
Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill.
Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out. The formation of nanoparticles was confirmed by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UV-Vis spectrophotometric analysis. The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line.
Results: The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 425 nm. X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 15-40 nm with an average size of 32 nm. Silver particles are spherical in shape, clustered. The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPS also showed cytotoxicity on ovarian cancer cell line PA-1 and lung epithelial cancer cell line A549.
Conclusion: The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated...ijtsrd
Biosynthesis of silver nanoparticles is an eye catching area of a modern research field. It is simple, single step, non toxic and eco friendly method. In the present investigation, the aqueous extract of Bauhinia tomentosa is used to synthesise silver nanoparticles and it was confirmed by UV Visible spectrometer. Due to surface Plasmon resonance, the peak obtained at 440.0 nm. The functional group present at the surface of the silver nanoparticles was confirmed by FT IR analysis. Further, the synthesized silver nanoparticles were subjected to antibacterial activity by Well diffusion method and also antioxidant activity performed by hydrogen peroxide scavenging assay. As concentration of silver nanoparticles increases the bactericidal activity is raised and also the antioxidant activity is increased. Thus it reveals that due to presence of various bioactive compounds present in the plant extract acting as reducing agent to reduce the size of silver ions into silver nanoparticles. Therefore, the biosynthesized nanoparticles is utilized for various biomedical applications. Mohanapriya. P ""Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated Silver Nanoparticles using Aqueous Extract of Bauhinia Tomentosa"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd22780.pdf
Paper URL: https://www.ijtsrd.com/engineering/nano-technology-/22780/invitro-assessment-of-antibacterial-and-antioxidant-property-of-biofabricated-silver-nanoparticles-using-aqueous-extract-of-bauhinia-tomentosa/mohanapriya-p
Abstract
A rapid advance of nanotechnology has the potential approach for significant improvements in disease prevention, diagnosis and treatment. In this article, we report a simple and eco-friendly biosynthesis of silver nanoparticles (Ag-NPs) using silver nitrate as metal precursor in Curcuma longa. These Ag-NPs were characterized by UV–vis spectroscopy, and Transmission electron microscopy (TEM). These nanoparticles exhibited maximum absorbance in specific nano meter range in UV–vis spectroscopy. TEM micrographs revealed the formation of well-dispersed Ag-NPs with its size and morphology. Microbiology assay founds that Ag-NPs are effective against V.cholera bacteria. These developments raise exciting opportunities to diagnose and treat pathogenic mode of infection based on the various profiles to target diseases.
Synthesis of the silver nanoparticles from Aloe barbadensis extract and its a...IRJET Journal
This document summarizes a study that synthesized silver nanoparticles from Aloe barbadensis leaf extract and evaluated their antimicrobial activity against urinary tract infection pathogens. Key findings include:
- Silver nanoparticles were successfully synthesized using Aloe barbadensis leaf extract and characterized using UV-Vis spectroscopy and FTIR.
- Thirty-two bacteria isolated from urine samples were identified, with Escherichia coli being the most prevalent pathogen identified in 47% of samples.
- Disc diffusion assays found the silver nanoparticles showed strongest antimicrobial activity against E. coli, followed by Candida albicans. Klebsiella pneumoniae and Staphylococcus aureus showed no sensitivity.
- Results indicate silver
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The document summarizes research characterizing gold nanoparticles synthesized intracellularly by the biomass of Aspergillus terreus fungus. When an aqueous solution of chloroauric acid was reduced by A. terreus biomass, it changed color from yellow to pinkish violet, indicating gold nanoparticle formation. The synthesized nanoparticles were spherical or irregularly shaped with an average size of 186 nm. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction were used to characterize the nanoparticles and confirmed the presence of crystalline gold nanoparticles capped by biomolecules from the fungus.
This document discusses the topic of nanobioremediation. It begins with introductions to nanoscience and bioremediation. Nanobioremediation uses nanoscience and nanoparticles to enhance microbial activity and remove pollutants more quickly. Examples are given of using genetic engineering and nanoparticles to modify microbes for improved bioremediation. The document concludes that nanobioremediation can clean environmental hazards faster and safer than other methods while meeting economic, social, and environmental criteria.
Biosynthesis of Silver Nanoparticles (AgNPs) and it's ApplicationsManish Dash
The document discusses the green synthesis and applications of silver nanoparticles. It begins by outlining the need for advanced disinfectant nanomaterials to prevent disease outbreaks caused by overpopulation and poor sanitation. It then describes how silver nanoparticles are a promising material for developing antimicrobial products due to their high antimicrobial activity. The document goes on to detail a green synthesis method for producing silver nanoparticles using the extract of Bougainvillea plant bracts, and characterizes the nanoparticles. It finds that the synthesized silver nanoparticles demonstrate effective antimicrobial properties against bacteria as well as antifouling effects.
The document summarizes a study that biologically synthesized and characterized intracellular gold nanoparticles using the biomass of Aspergillus fumigatus. A. fumigatus was grown in liquid culture and its biomass was then exposed to chloroauric acid solution, resulting in the intracellular production of gold nanoparticles within 72 hours, as indicated by a color change. The synthesized nanoparticles were characterized through various techniques and were found to be spherical and irregularly shaped, ranging from 85.1 to 210 nm in size. Scanning electron microscopy revealed the nanoparticles accumulated on the fungal mycelia. Energy dispersive spectroscopy and X-ray diffraction confirmed the presence and crystalline nature of the synthesized gold nanoparticles.
This document summarizes a study that used the leaves of lemongrass (Cymbopogan citratus) to rapidly synthesize silver nanoparticles. Silver nanoparticles formed within 8-10 minutes when a lemongrass leaf extract reacted with silver nitrate under microwave irradiation. The nanoparticles were characterized using UV-visible spectroscopy, nanoparticle tracking analysis, transmission electron microscopy, and energy dispersive X-ray spectroscopy. The silver nanoparticles showed antimicrobial activity against bacteria such as E. coli and S. aureus as well as fungi such as C. albicans, inhibiting their growth in laboratory assays. When combined with antibiotics, the silver nanoparticles enhanced the antibiotics' effectiveness against drug-resistant microbial strains.
This study evaluated the biosorption of zinc and nickel from wastewater using dead fungal biomass of Aspergillus flavus. The maximum biosorption capacity for zinc was found to be 47.36% at pH 6.5, with a biomass concentration of 2g/L, contact time of 50 minutes, and zinc concentration of 2ppm. For nickel, the maximum biosorption capacity was 61.60% at pH 5, with a biomass concentration of 2g/L, contact time of 60 minutes, and nickel concentration of 2ppm. Desorption using 0.1M HCl was found to be effective in removing zinc and nickel from the fungal biomass,
Nanotechnology in cancer and its synthesisShreyaBhatt23
basic introduction to nanotechnology and the types of nanomaterials used in medical purpose. sysnthesis of nanomaterials by physical , chemical, biosynthesis, green synthesis of nanomaterials
Bio synthesis of nano particles using bacteriaudhay roopavath
Bacteria can be used to biosynthesize nanoparticles through intracellular and extracellular methods. Intracellular synthesis occurs inside the cell, where bacteria reduce metal ions and deposit nanoparticles in locations like the periplasmic space. Extracellular synthesis involves enzymes secreted by bacteria reducing metal ions outside the cell and precipitating nanoparticles. Examples are given of bacteria producing silver, titanium, zinc sulfide and lead sulfide nanoparticles through extracellular and intracellular pathways. While a green approach, bacterial nanoparticle synthesis can be slow with difficulty controlling size, shape and crystallinity of particles.
Role of salt precursor in the synthesis of zinc oxide nanoparticleseSAT Journals
Abstract In this paper, Zinc oxide nanoparticles having wurtzite crystalline structure are synthesized. The temperature, base concentration and the salt precursor used for the synthesis affects the morphology and particle size. The synthesized nanoparticles are characterized by X-ray diffraction, Scanning electron microscopy and Diffused reflectance UV-visible spectroscopy. As the temperature is increased from 800 C to 1000C, keeping the concentration of the base viz sodium hydroxide at 5M, the particle size increases from 30nm to 500nm. With change in base concentration from 2M to 10M, at constant reaction temperature of 800C, the particle size increases from 30 nm to 500 nm. Herein the precursor used is zinc chloride. The effect of salt precursor is studied for three different salt precursors, viz. zinc chloride, zinc nitrate and zinc acetate. This paper is an attempt to give the information about salt precursor to be used, optimum values of temperature and base concentration for synthesis of the ZnO nanoparticles with enhanced antibacterial property for suitable biomedical application. Keywords: Zinc Oxide nanoparticles, metal oxide nanoparticles, nanotechnology.
Role of salt precursor in the synthesis of zinc oxide nanoparticleseSAT Publishing House
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
The document summarizes the biosynthesis of copper nanoparticles using Beta Vulgaris extract and their antibacterial properties. Key points:
1) Beta Vulgaris extract was used to biosynthesize copper nanoparticles due to its abundance of stabilizing compounds like betalains.
2) Characterization techniques like UV-Vis, SEM, EDAX, and FTIR confirmed the formation of mono-dispersed copper nanoparticles around 35-60 nm in size.
3) Disc diffusion tests showed the biologically synthesized copper nanoparticles had good antibacterial activity against various bacterial strains like E. coli, with the greatest inhibition of E. coli growth.
Antimicrobial and cytotoxicity effect of silver nanoparticle synthesized by C...Nanomedicine Journal (NMJ)
Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill.
Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out. The formation of nanoparticles was confirmed by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UV-Vis spectrophotometric analysis. The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line.
Results: The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 425 nm. X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 15-40 nm with an average size of 32 nm. Silver particles are spherical in shape, clustered. The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPS also showed cytotoxicity on ovarian cancer cell line PA-1 and lung epithelial cancer cell line A549.
Conclusion: The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated...ijtsrd
Biosynthesis of silver nanoparticles is an eye catching area of a modern research field. It is simple, single step, non toxic and eco friendly method. In the present investigation, the aqueous extract of Bauhinia tomentosa is used to synthesise silver nanoparticles and it was confirmed by UV Visible spectrometer. Due to surface Plasmon resonance, the peak obtained at 440.0 nm. The functional group present at the surface of the silver nanoparticles was confirmed by FT IR analysis. Further, the synthesized silver nanoparticles were subjected to antibacterial activity by Well diffusion method and also antioxidant activity performed by hydrogen peroxide scavenging assay. As concentration of silver nanoparticles increases the bactericidal activity is raised and also the antioxidant activity is increased. Thus it reveals that due to presence of various bioactive compounds present in the plant extract acting as reducing agent to reduce the size of silver ions into silver nanoparticles. Therefore, the biosynthesized nanoparticles is utilized for various biomedical applications. Mohanapriya. P ""Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated Silver Nanoparticles using Aqueous Extract of Bauhinia Tomentosa"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd22780.pdf
Paper URL: https://www.ijtsrd.com/engineering/nano-technology-/22780/invitro-assessment-of-antibacterial-and-antioxidant-property-of-biofabricated-silver-nanoparticles-using-aqueous-extract-of-bauhinia-tomentosa/mohanapriya-p
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Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
Presentation in the Science Coffee of the Advanced Concepts Team of the European Space Agency on the 07.06.2024.
Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.
The cost of acquiring information by natural selectionCarl Bergstrom
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
2. About Nanoparticles
Word ‘Nano’ comes from Greek word ‘nanos’ meaning dwarf. These are tiny
particles with dimensions on the nanometer scale (1-100 nanometers). They
exhibit unique properties due to their small size and high surface area-to-
volume ratio, making them valuable in various applications such as
medicine, electronics, and materials science.
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3. Approaches for the synthesis of nanomaterials
• The “top-down” approach, which involves the breaking
down of large pieces of material to generate the
required nanostructures from them.
• Bottom-up approach in nanotechnology is making larger
nanostructures from smaller building blocks such as
atoms and molecules. Self assembly in which desired
nano structures are self assembled without any external
manipulation.
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4. Biological Synthesis (Green synthesis)
It is a rapid, ecofriendly, and easily scaled-up technology.
Why Green Synthesis?
• Easy.
• Efficient.
• Eco- Friendly.
• Eliminates use of toxic chemicals.
• Consume less energy.
• Produce safer products and by products.
• Does not impart any hazardous effect on environment.
4
5. Biological Synthesis of Nanoparticles by Microorganisms
(Bacteria)
• Bacteria has been most extensively researched for synthesis of nanoparticles because of
their fast growth and relative ease of genetic manipulation.
• Intracellular – Inside the cell, in cytoplasm.
• Extracellular – Outside the cell on the surface or between the cells inside a colony.
Methods of synthesis
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6. • Mechanism
Uptake of Metal Ions
The cell takes up metal ions,
often from the surrounding
environment, through
transporters or diffusion.
Bio-Reduction
Intracellular reduction processes
occur due to the presence of
enzymes, co-factors, and other
biomolecules. These facilitate the
conversion of metal ions into
nanoparticles.
Nucleation and Growth
Nucleation of nanoparticle
seeds occurs, followed by the
controlled growth of
nanoparticles within the
cellular confines.
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7. Microbial production of silver nanoparticles
It was investigated using the bacterial strain Escherichia coli.
Method
The bacterial strain E.coli were cultured, for broth medium and LB
medium. The culture flasks were incubated on an orbital shaker at
27°C and agitated at 220 rpm. The biomass was harvested after 24
hours of growth and centrifuged at 12000 rpm for 10 minutes.
Synthesis of Silver nanoparticles
The sample added separately to the reaction vessel containing
silver nitrate (AgNO3) at specific concentration and control was
also run along with the experimental condition. The primary
conformation of synthesis of nanoparticles in the medium was
characterized by the changes in color from yellowish white to
brown.
Production of biomass
7
8. Microbial production of silver nanoparticles
Characterization of silver nanoparticles
The bioreduction of the Ag+ ions in the solution was
monitored and sample of 2ml was withdrawn at
different time intervals and the absorbance was
measured using UV–visible spectrophotometer with
samples in quartz cuvette.
Particle sizing measurements
Particle size analyzing experiments were carried out
by means of laser diffractometry. Measurements
were taken in the range between 0.04 up to 500 μm.
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9. Microbial production of Zn nanoparticles
• ZnO nanoparticles are produced intracellular by bacteria such as Lactobacillus.
Methods
Zn Solution preparation
1 M stock solution for Zn2+ is prepared. Then
solution Zn2+ was filtered into the bacterial culture
medium and added.
Isolation of lactic acid bacteria
Using (MRS) medium, lactic acid bacteria was isolated from Cow
milk and incubated. The streak plate technique was used to
obtain single colonies with various morphologies.
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10. Microbial production of Zn nanoparticles
Tolerance determination
Determine the NaCl tolerance and bile
salt tolerance of isolated lactobacillus.
Biosynthesis of ZnO nanoparticles
The cell biomass then suspended in sterilized deionized water containing
Zn2+ at concentrations and incubated. The cells were collected at 5000
rpm for 10 minutes by centrifugation and washed with saline before being
hung in a buffer. Cells were interrupted by alternating ultrasound cycles in
100 W for 5 minutes to get the ZnO nanoparticles.
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12. Extracellular synthesis
• It includes bio-mineralization, bio sorption or precipitation.
• Here cell wall reductive enzymes or soluble secreted enzymes are involved in the
reductive process of metal ions.
• With the change in pH of the solution, various shapes and sizes were formed.
The culture supernatants of Enterobacteria like Klebsiella pneumonia, E.coli, Enterobacter
cloaceae rapidly synthesize silver nanoparticles by reducing Ag+ to Ag.
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13. Microbial production of Au nanoparticles
Materials and Reagents:
• Bacterial Culture Preparation: Inoculate a culture flask with the selected bacterial strain
and incubate it overnight at the optimal temperature and pH for growth.
• Growth Medium Adjustment: Prepare a fresh growth medium with the gold precursor
(e.g., HAuCl4). Adjust the pH of the medium to promote the reduction of gold ions.
• Incubation with Gold Precursor: Add the gold-containing medium to the bacterial
culture flask.
Incubate the flask with constant shaking to ensure uniform mixing of the medium and
bacterial culture.
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14. Microbial production of Au nanoparticles
Monitoring and Optimization: Periodically sample the solution and measure the changes
in color. The development of a ruby red or purple color is indicative of AuNP formation.
Biomass Separation: Centrifuge the culture to separate the bacterial biomass from the
AuNPs and supernatant.
Characterization: Characterize the synthesized AuNPs using Transmission Electron
Microscopy (TEM), UV-Vis spectroscopy, X-ray Diffraction (XRD), and Dynamic Light
Scattering (DLS) to analyze size, shape, and other properties.
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15. Intracellular and Extracellular Synthesis of nanoparticles
Aspect Intracellular Synthesis Extracellular Synthesis
Location of Synthesis Inside the microbial cell Outside the microbial cell
Microbial Synthesis Bacteria, fungi, algae Bacteria and fungi
Key Process Reduction of metal ions within the
cell.
Reduction of metal ions
outside the cell.
Control over size and
shape
Good control, can be precise. Limited control, more
challenging.
Biocompatibility High biocompatibility due to
nanoparticles formed inside cells.
Depends on presence of any
toxic byproducts.
Purification and
collection
Require additional steps for cell lysis
and purification
Easier to separate
nanoparticles from the
extracellular medium.
Applications Drug delivery, catalysis and sensing. Drug delivery, catalysis, sensing
and nanomedicine.
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16. Synthesis of nanoparticles by fungi
• Fungi and Yeast are very effective secretors of extracellular enzymes.
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17. Potential fungal isolates used for biosynthesis of nanoparticles.
Biogenic synthesis of nanoparticles using
fungi
• Intracellular and Extracellular.
In intracellular synthesis, the metal precursor is
added to the mycelial culture and is internalized
in the biomass. Extraction of the nanoparticles is
required after the synthesis, employing chemical
treatment, centrifugation, and filtration to disrupt
the biomass and release the nanoparticles
In extracellular synthesis, the metal precursor
is added to the aqueous filtrate containing only
the fungal biomolecules, resulting in the
formation of free nanoparticles in the
dispersion.
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18. Biosynthesis of Zinc Oxide Nanoparticles using Fungus
a. Fungi like Aspergillus,
Penicillium etc. transferring
onto a fresh agar plate and
allowing it to grow.
b. Once the culture is
established, transfer it to
Erlenmeyer flask. Incubate the
flask with agitation (if using a
shaker) until the fungus
reaches the desired growth
phase.
a. When the fungal culture
has grown, add a known
volume or concentration of
the zinc precursor solution to
the fungal culture medium.
b. Incubate the culture
mixture under appropriate
conditions (temperature, pH,
and agitation if necessary) for
a specified period.
Dissolve the selected
zinc salt (e.g.,
Zn(NO3)2 or ZnSO4) in
deionized water to
prepare a stock
solution of known
concentration.
The formation of Zn nanoparticles is often indicated by a color change from colorless or pale to brown
or reddish-brown.
Use techniques like UV-Visible spectroscopy to measure the absorbance spectrum, which can provide
information about the formation of nanoparticles.
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20. Biosynthesis of Silver Nanoparticles using Fungus
Isolation and Culturing of
Fungi:
a. Isolate and identify a
suitable
fungal strain. Common strains
used include Aspergillus,
Fusarium, and Trichoderma.
b. Cultivate the selected fungal
strain on a suitable growth
medium under controlled
conditions .Allow the fungus to
grow until it reaches the
desired biomass.
Preparation of Silver
Nitrate Solution:
Prepare a stock
solution of silver nitrate
(AgNO3) by dissolving a
known amount in Milli-
Q water. This solution
will be used as a source
of silver ions (Ag+).
Inoculation and Incubation:
a. Add the fungal culture to a
flask containing a growth
medium.
b. To this flask, add silver nitrate
solution.
c. Adjust the pH of the mixture
(typically pH 7-8).
d. Incubate the mixture in an
incubator at a controlled
temperature and allow the
reaction to proceed. Ag+ ions
will be reduced by the fungal
biomass to form silver
nanoparticles
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21. Biosynthesis of Gold Nanoparticles using fungi
Isolate and Culture the Fungus: Isolate a pure culture of the selected fungus.
• Grow the fungus on a suitable culture medium in a sterile environment, following
standard microbiological techniques.
Prepare Fungal Biomass: Allow the fungus to grow until it reaches the logarithmic growth
phase.
• Harvest the fungal biomass by centrifugation and wash it thoroughly to remove any
culture medium components.
Gold Salt Reduction: Prepare a gold salt solution (e.g., HAuCl4) in deionized water.
• Add the fungal biomass to the gold salt solution and incubate the mixture at a controlled
temperature, typically around 25-30°C.
• Over time, the fungal biomass will reduce the gold ions to form AuNPs. The color of the
solution will change from pale yellow to a characteristic red or purple, indicating the
formation of AuNPs
21
22. Characterization: Measure the absorbance spectrum of the reaction mixture using a
spectrophotometer. AuNPs typically exhibit a strong absorbance peak in the visible range,
around 520-550 nm.
• Analyze the size and shape of the synthesized AuNPs using techniques such as
transmission electron microscopy (TEM) or dynamic light scattering (DLS).
Purification: Centrifuge the reaction mixture to separate the fungal biomass and any
unreacted gold ions from the AuNPs.
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23. References
• EL-GHWAS, D. E. (2022). Characterization and biological synthesis of zinc oxide nanoparticles by new strain
of Bacillus foraminis. Biodiversitas Journal of Biological Diversity, 23(1).
• Suba, S., Vijayakumar, S., Vidhya, E., Punitha, V. N., & Nilavukkarasi, M. (2021). Microbial mediated
synthesis of ZnO nanoparticles derived from Lactobacillus spp: Characterizations, antimicrobial and
biocompatibility efficiencies. Sensors International, 2, 100104.
• Moormann, G. C., & Bachand, G. D. (2021). Biosynthesis of Zinc Oxide Nanoparticles using Fungal Filtrates
(No. SAND2021-9437R). Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Center
for Integrated Nanotechnologies (CINT).
• Guilger-Casagrande, M., & Lima, R. D. (2019). Synthesis of silver nanoparticles mediated by fungi: a review.
Frontiers in bioengineering and biotechnology, 7, 287.
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