The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Effluents containing heavy metals can be
remediated with the help of dead microorganisms by the process
known as biosorption. In this study the dead biomass 1of fungus
Aspergillus flavus was used for the biosorption of heavy metals
i.e., Zinc and Nickel. The capacity of biosorption by the dead
biomass of Aspergillus flavus was evaluated at room temperature
with different parameters which are; pH, contact time, biomass
concentration and metal ion concentration. The biosorption
capacity for Zn was found to be 47.36% at room temperature, at
pH 6.5, with biomass concentration of 2g/L having contact time of
50 min and solution concentration of 2ppm. Biosorption capacity
for Ni was found to be 61.60% at room temperature, at pH 5,
with biomass concentration of 2g/L having contact time of 60 min
and solution concentration of 2ppm. . In this study, desorption of
the heavy metals by 0.1M HCl was found to be effective. Fungal
biomass was recovered for reuse.
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Effluents containing heavy metals can be
remediated with the help of dead microorganisms by the process
known as biosorption. In this study the dead biomass 1of fungus
Aspergillus flavus was used for the biosorption of heavy metals
i.e., Zinc and Nickel. The capacity of biosorption by the dead
biomass of Aspergillus flavus was evaluated at room temperature
with different parameters which are; pH, contact time, biomass
concentration and metal ion concentration. The biosorption
capacity for Zn was found to be 47.36% at room temperature, at
pH 6.5, with biomass concentration of 2g/L having contact time of
50 min and solution concentration of 2ppm. Biosorption capacity
for Ni was found to be 61.60% at room temperature, at pH 5,
with biomass concentration of 2g/L having contact time of 60 min
and solution concentration of 2ppm. . In this study, desorption of
the heavy metals by 0.1M HCl was found to be effective. Fungal
biomass was recovered for reuse.
Term ‘Nano’ comes from the Greek word ‘nanos’ meaning dwarf and denotes a measurement on the scale of one billionth (10⁹) of a meter in size. Nanoparticles are defined as a particulate dispersions of solid particles with atleast one dimension at a size range of 10-1000 nm. The most important feature of Nanoparticles is their surface area to volume aspect ratio, allowing them to interact with other particles easier.
ABSTRACT In the present study, the extracellular synthesis of Silver nanoparticles was done using two different bacterial strains viz. Bacillus flexus and Bacillus pseudomycoides. The silver nanoparticles were confirmed by in color test and characterized by UV-Visible spectroscopy, the λmax observed at 430 nm and 410 nm confirmed the synthesis of AgNPs. FTIR analysis confirms the presence of elemental silver and reveals the dual function of the biological molecule responsible for the reduction and stabilization of AgNPs in the aqueous medium. The XRD showed that silver nanoparticles produced are crystalline in nature with size ranges from 30 to 70 nm. The SEM shows that produced silver nanoparticles are spherical, Pseudo spherical in shape with traces of agglomeration. Further through investigation of Antibiotic Sensitivity/Resistant pattern expressed that out of eighteen virulent enteric bacterial isolates, three isolates showed MAR index equal to 1, which indicates the presence of multiple drug resistance (MDR). MIC values of AgNPs against MDR isolate E7 and K3 was established to be 80 μg/ml whereas, for isolate Sa1 the MIC value was 70 μg/ml. The synergistic effect of antibiotics in conjugation with biologically synthesized AgNPs encourage the susceptibility amongst the tested bacterial cultures; viz. Salmonella followed by Klebsiella and E. coli.
Key-words: Biosynthesis, Synergistic activity, Antibacterial activity, Silver nanoparticles, Multidrug-resistant (MDR)
Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract a...ijtsrd
The main objective of the present study is to deal with the green synthesis of Zinc Oxide [ZnO] nanoparticles utilizing aqueous leaf extract of Jasminum Fluminense.` Zinc acetate [Zn(O2CCH3)2(H2O)2] and sodium hydroxide [NaOH] were used as starting materials and Jasminum Fluminense [Nithyamalli] leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet [UV] “ Visible Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. Formation of ZnO nanoparticles has been confirmed by UV-visible spectroscopy and the TEM analysis spectacles that the synthesized ZnO nanoparticles are of face centered cubic (fcc) structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This simplistic and green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedical science because it is environmentally friendly and does not involve any harmful substances. S. Amudha"Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12908.pdf http://www.ijtsrd.com/physics/nanotechnology/12908/green-synthesis-of-zno-nanoparticles-using-jasminum-fluminense-leaf-extract-and-its-spectral-and-optical-characterization-studies/s-amudha
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Biological method for the preparation of nanoparticles(Sheersho)Sheersha Pramanik 🇮🇳
I have described about the biological processes(other than physical,chemical) for the preparation of Nanoparticles.
do like comment share if you like it.
ABSTRACT- In our present study, we account for eco-friendly biosynthesis of copper nanoparticles using aqueous leaves extract of Artocarpus heterophyllus against first to fourth instar larvae of Aedes aegypti. The synthesized CuNPs were characterized by UV, XRD, FTIR and SEM analyses were clearly distinguishable. The four different immature mosquito larval stages of A. aegypti were exposed to varying concentrations of aqueous leaf extract of A. heterophyllus, copper sulphate (CuSO4) and synthesized CuNPs for 24 h. The mortality was observed at aqueous extract (LC50= 48.40, 60.55, 70.36, and 82.79 mg/ml), CuSO4 (LC50=21.81, 26.92, 41.38, and 55.12 mg/ml) synthesized CuNPs against first to fourth instars of A. aegypti (LC50= 3.85, 4.24, 4.66, and 5.08 mg/ml), respectively. The novel properties created not only improve the quality of human’s life; it also helps in saving energy and environment.
Key-words: Copper nanoparticles, Jackfruit, Aedes aegypti, aqueous leaf extract
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Term ‘Nano’ comes from the Greek word ‘nanos’ meaning dwarf and denotes a measurement on the scale of one billionth (10⁹) of a meter in size. Nanoparticles are defined as a particulate dispersions of solid particles with atleast one dimension at a size range of 10-1000 nm. The most important feature of Nanoparticles is their surface area to volume aspect ratio, allowing them to interact with other particles easier.
ABSTRACT In the present study, the extracellular synthesis of Silver nanoparticles was done using two different bacterial strains viz. Bacillus flexus and Bacillus pseudomycoides. The silver nanoparticles were confirmed by in color test and characterized by UV-Visible spectroscopy, the λmax observed at 430 nm and 410 nm confirmed the synthesis of AgNPs. FTIR analysis confirms the presence of elemental silver and reveals the dual function of the biological molecule responsible for the reduction and stabilization of AgNPs in the aqueous medium. The XRD showed that silver nanoparticles produced are crystalline in nature with size ranges from 30 to 70 nm. The SEM shows that produced silver nanoparticles are spherical, Pseudo spherical in shape with traces of agglomeration. Further through investigation of Antibiotic Sensitivity/Resistant pattern expressed that out of eighteen virulent enteric bacterial isolates, three isolates showed MAR index equal to 1, which indicates the presence of multiple drug resistance (MDR). MIC values of AgNPs against MDR isolate E7 and K3 was established to be 80 μg/ml whereas, for isolate Sa1 the MIC value was 70 μg/ml. The synergistic effect of antibiotics in conjugation with biologically synthesized AgNPs encourage the susceptibility amongst the tested bacterial cultures; viz. Salmonella followed by Klebsiella and E. coli.
Key-words: Biosynthesis, Synergistic activity, Antibacterial activity, Silver nanoparticles, Multidrug-resistant (MDR)
Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract a...ijtsrd
The main objective of the present study is to deal with the green synthesis of Zinc Oxide [ZnO] nanoparticles utilizing aqueous leaf extract of Jasminum Fluminense.` Zinc acetate [Zn(O2CCH3)2(H2O)2] and sodium hydroxide [NaOH] were used as starting materials and Jasminum Fluminense [Nithyamalli] leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet [UV] “ Visible Spectroscopy, Fourier Transform Infrared Spectroscopy [FTIR] and Transmission Electron Microscopic [TEM] studies. Formation of ZnO nanoparticles has been confirmed by UV-visible spectroscopy and the TEM analysis spectacles that the synthesized ZnO nanoparticles are of face centered cubic (fcc) structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This simplistic and green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedical science because it is environmentally friendly and does not involve any harmful substances. S. Amudha"Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd12908.pdf http://www.ijtsrd.com/physics/nanotechnology/12908/green-synthesis-of-zno-nanoparticles-using-jasminum-fluminense-leaf-extract-and-its-spectral-and-optical-characterization-studies/s-amudha
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Biological method for the preparation of nanoparticles(Sheersho)Sheersha Pramanik 🇮🇳
I have described about the biological processes(other than physical,chemical) for the preparation of Nanoparticles.
do like comment share if you like it.
ABSTRACT- In our present study, we account for eco-friendly biosynthesis of copper nanoparticles using aqueous leaves extract of Artocarpus heterophyllus against first to fourth instar larvae of Aedes aegypti. The synthesized CuNPs were characterized by UV, XRD, FTIR and SEM analyses were clearly distinguishable. The four different immature mosquito larval stages of A. aegypti were exposed to varying concentrations of aqueous leaf extract of A. heterophyllus, copper sulphate (CuSO4) and synthesized CuNPs for 24 h. The mortality was observed at aqueous extract (LC50= 48.40, 60.55, 70.36, and 82.79 mg/ml), CuSO4 (LC50=21.81, 26.92, 41.38, and 55.12 mg/ml) synthesized CuNPs against first to fourth instars of A. aegypti (LC50= 3.85, 4.24, 4.66, and 5.08 mg/ml), respectively. The novel properties created not only improve the quality of human’s life; it also helps in saving energy and environment.
Key-words: Copper nanoparticles, Jackfruit, Aedes aegypti, aqueous leaf extract
Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica P...IIJSRJournal
In this work, Zinc Oxide (ZnO) and Mn-doped ZnO nanoparticles were green synthesized using Carica papaya extract by the Co-precipitation method. X-ray diffraction (XRD) results revealed the formation of ZnO and Mn-doped ZnO nanoparticles with the wurtzite crystal structure (hexagonal). Due to the presence of dopant Manganese (Mn) the optical spectra showed a redshift in the absorbance spectrum. Structural and optical properties of the end product showed that the manganese ions (Mn2+) substituted the Zinc ions (Zn2+) without altering the Wurtzite structure of ZnO. Fourier Transform Infrared Spectroscopy (FTIR) spectra confirm the presence of metal oxide present in the end product. The antibacterial efficiency of ZnO and Mn-doped ZnO nanoparticles were studied using the agar well diffusion method against Gram-positive and Gram–negative bacteria. It is obvious from the results that Mn doped ZnO nanoparticles exhibit better antibacterial activity than ZnO nanoparticles.
Silver Nanoparticles from a Plant Echinacea Purpurea Extract for its Antipath...ijtsrd
Nanotechnology is gaining tremendous impetus in the present century due to its capability of modulating metals into their nanosize. The synthesis, characterization, and application of biologically synthesized nanomaterials have become an important branch of nanotechnology. Research in nanotechnology highlights the possibility of green chemistry pathways to produce technologically important nanomaterials. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are often toxic and flammable. Silver nanoparticles are the metal of choice as they hold the promise to kill microbes effectively. The present study describes a cost effective and environment friendly technique for green synthesis of silver nanoparticles from 1mM silver nitrate solution through the a plant Echinacea purpurea cone flower extract. The appearance of brown colour indicates the synthesis of silver nanoparticles. Nanoparticles were characterized using UV Vis absorption spectroscopy and SEM analysis. UV Vis spectrum of the aqueous medium containing silver nanoparticles showed absorption peak at 450nm. SEM analysis showed the average particle size of 50 70nm and spherical shape of the silver nanoparticles. Further studies on the silver nanoparticles showed that it has the antibacterial activities. Antipathogenic activity study was carried out by spread plate, pour plate on Escherichia coli and disc diffusion methods on pathogenic organisms such as Escherichia coli, Proteus vulgaricus, Klebsiella pneumoniae, Pseudomonas aeruginosa. Compared to spread plate, pour plate method showed the maximum antibacterial activity. Zone of inhibition was observed by disc diffusion methods and among these four pathogens, Klebsiella pneumoniae and Escherichia coli showed the maximum activity. Satheesh Kumar B. "Silver Nanoparticles from a Plant Echinacea Purpurea Extract for its Antipathogenic Efficacy" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-6 , October 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50001.pdf Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/50001/silver-nanoparticles-from-a-plant-echinacea-purpurea-extract-for-its-antipathogenic-efficacy/satheesh-kumar-b
Abstract
A rapid advance of nanotechnology has the potential approach for significant improvements in disease prevention, diagnosis and treatment. In this article, we report a simple and eco-friendly biosynthesis of silver nanoparticles (Ag-NPs) using silver nitrate as metal precursor in Curcuma longa. These Ag-NPs were characterized by UV–vis spectroscopy, and Transmission electron microscopy (TEM). These nanoparticles exhibited maximum absorbance in specific nano meter range in UV–vis spectroscopy. TEM micrographs revealed the formation of well-dispersed Ag-NPs with its size and morphology. Microbiology assay founds that Ag-NPs are effective against V.cholera bacteria. These developments raise exciting opportunities to diagnose and treat pathogenic mode of infection based on the various profiles to target diseases.
Cytotoxic | Primary research | Silver nanoparticlesPubrica
Biosynthesis, Antimicrobial, and Cytotoxic Effects of Silver Nanoparticles Using Acacia Concinna POD Extract and Kigelia Africa Leaf Extract- Secondary metabolites found in plants include alkaloids, flavonoids, phenolic compounds, phytosterols, saponins, tannins, carbohydrates, proteins, lipids, and minerals. These secondary metabolites have a wide range of uses, including the production of nanoparticles.
Biosynthesis, Antimicrobial, and Cytotoxic Effects of Silver Nanoparticles Using Acacia Concinna POD Extract and Kigelia Africa Leaf Extract- Secondary metabolites found in plants include alkaloids, flavonoids, phenolic compounds, phytosterols, saponins, tannins, carbohydrates, proteins, lipids, and minerals. These secondary metabolites have a wide range of uses, including the production of nanoparticles.
Visit us @ https://pubrica.com/insights/sample-work/cytotoxic-effects-of-sliver-nanoparticles-using-leaf-extract/
Green synthesis and characterization of silver nanoparticles using tinosopora...IJARIIT
User authentication is an important topic in the field of information security. To enforce security of information,
passwords were introduced. Text based password is a popular authentication method used from ancient times. However text
based passwords are prone to various attacks. Strong text-based password schemes could provide with certain degree of security.
However, the fact that strong passwords are difficult to memorize often leads their users to write them down on papers or even
save them in a computer file. Human actions such as choosing bad passwords and inputting passwords in an insecure way are
regarded as the weakest link in the authentication chain. Graphical password is one of the alternative solution to alphanumeric
password as it is very simple process to remember alphanumeric password. One of the major reasons behind this method
implementation is that, according to psychological studies human mind can easily remember images than alphabets or digits.
Graphical authentication has been proposed as a possible alternative solution to text-based authentication. A new technique of
captcha and OTP is being used for the verification purpose. Three times a person is given chance to try for login if the person
fails then he is blocked till the session expires.
Formulation Of Acalypha Wilkesiana Muell. Arg. Ethanol Leaf Extract into Crea...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Antimicrobial and cytotoxicity effect of silver nanoparticle synthesized by C...Nanomedicine Journal (NMJ)
Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill.
Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out. The formation of nanoparticles was confirmed by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UV-Vis spectrophotometric analysis. The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line.
Results: The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 425 nm. X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 15-40 nm with an average size of 32 nm. Silver particles are spherical in shape, clustered. The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPS also showed cytotoxicity on ovarian cancer cell line PA-1 and lung epithelial cancer cell line A549.
Conclusion: The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.
The current research aimed at fabricating plant extract mediated biosynthesized silver nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a new greener approach or technology to formulate anti-bacterial nanodrugs in future.
Synthesis and evaluation of bactericidal properties of CuO nanoparticles agai...Nanomedicine Journal (NMJ)
Objective(s):
CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species.
Materials and Methods:
Here, we synthesized CuO nanoparticles (NPs) and explored the antibacterial activity of CuO NPs preparation.
Results:
Single crystalline nanoparticles of copper oxide having almost uniform particle size of 5-6 nm has been synthesized by a facile and versatile route. XRD spectra confirmed the formation of single phase CuO NPs. Transmission electron microscopy results corroborate well with XRD results. The technique employed is free from toxic solvents, organics and amines, is based on a simple reaction of copper sulfate and de-ionized water (DI), and their bactericidal effects against of Aeromonas hydrophila ATCC 7966T bacteria were investigated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with liquid culture for all of the Aeromonas hydrophila culture Medias was done.
Conclusion:
Present study confirms that Copper oxide nanoparticles have great promise as antimicrobial agent against Aeromonas hydrophila.
Crimson Publishers-Synthesis, Characterization, and Evaluation of the In-vitr...CrimsonpublishersMedical
Cinnamomum zeylanicum have lot-of biological activities including antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents were identified throughout its biological properties. Bark of Cinnamomum zeylanicum (Lauraceae) collected respectively at Nanotechnology laboratory (ANGRAU, Tirupathi, India). In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. Stable Zn nanoparticles were formed by treating 90ml of 1mm zinc nitrate aqueous solution with 10ml of 10% bark extract. The formation of Cinnamomum zeylanicum bark extract mediated zinc nanoparticles (CZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 270nm.
Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated...ijtsrd
Biosynthesis of silver nanoparticles is an eye catching area of a modern research field. It is simple, single step, non toxic and eco friendly method. In the present investigation, the aqueous extract of Bauhinia tomentosa is used to synthesise silver nanoparticles and it was confirmed by UV Visible spectrometer. Due to surface Plasmon resonance, the peak obtained at 440.0 nm. The functional group present at the surface of the silver nanoparticles was confirmed by FT IR analysis. Further, the synthesized silver nanoparticles were subjected to antibacterial activity by Well diffusion method and also antioxidant activity performed by hydrogen peroxide scavenging assay. As concentration of silver nanoparticles increases the bactericidal activity is raised and also the antioxidant activity is increased. Thus it reveals that due to presence of various bioactive compounds present in the plant extract acting as reducing agent to reduce the size of silver ions into silver nanoparticles. Therefore, the biosynthesized nanoparticles is utilized for various biomedical applications. Mohanapriya. P ""Invitro Assessment of Antibacterial and Antioxidant Property of Biofabricated Silver Nanoparticles using Aqueous Extract of Bauhinia Tomentosa"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd22780.pdf
Paper URL: https://www.ijtsrd.com/engineering/nano-technology-/22780/invitro-assessment-of-antibacterial-and-antioxidant-property-of-biofabricated-silver-nanoparticles-using-aqueous-extract-of-bauhinia-tomentosa/mohanapriya-p
Enlargement of biologically stimulated
investigational processes for the synthesis of nanoparticles is
budding into an important branch of nanotechnology. Eco
responsive methods of green mediated synthesis of nanoparticles
are the present research in the extremity of nanotechnology. The
bioreduction behavior of leaf extracts of Morinda citrifolia L.
(Rubiaceae) in the green synthesis of silver nanoparticles was
investigated employing UV/Visible Spectrophotometry, Particle
size analyzer, Zeta potential, Filed emission scanning electron
microscopy, Energy Dispersive X-ray Analysis and FourierTransform
Infrared Spectroscopy. The antifungal property of the
silver nanoparticles was tested against Candida albicans,
Candida tropicalis and Candida krusei. The Antifungal assay
tests Zone of inhibition revealed the concentrations of more than
10µl of silver nanoparticles were inhibited the growth of fungal
pathogens.
Similar to Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activit (20)
IPQC and FPQC test for opthalmic preparation as per IP, BP ANIPQC D USProshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball . These product must be sterile and are prepare under the same condition as that of parenteral preparation
Differential spectrophotometric method for estimation and validation of Verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, beeers law limits 5-25µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r 2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamine in simple, precise and economically and it recommended for the routine drug quality analysis investigation.
Formulation and Evaluation of Herbal Toothpaste: Compared with marketed prepa...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability-Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
Formulation and Evaluation of Herbal Toothpaste: Compared with marketed prepa...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability-Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
Formulation and Evaluation of Tooth Gel from Aloe vera leaves extractroshan telrandhe
The aimed of current research to formulate tooth gel utilizing leaf extract of Aloe vera. In multiple clinical studies, Aloe vera has used in dentistry for wound-healing effect, gingivitis, plaque control and curing oral mucosal lesions. Aloe vera is natural, ancient ingredient. The formulated Aloe vera tooth gel evaluated by physical examination: Colour-yellowish brown, Appearance-Homogeneous, smooth nature, Transparency-translucent and Relative density-10.5, No microbial growth in sample plate, pH7.5, Viscosity-310000cp, Extrudability amount percent-91.33, Spreadability-6.5cm/sec and obsereved good stability. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated aloe vera tooth gel exhibited notable activity with ZOI of 19.5 mm at MIC of 25μg/mL. The outcome from this research evidently signified that the natural plant Aloe vera using to formulate tooth gel may be a new approach to formulate tooth gel economically and minimum side effect than synthetic formulation and good scope in future about dental research in natural remedies.
Nanotechnology for Cancer therapy: Recent developmentsroshan telrandhe
This paper is an overview of advances and prospects in applications of nanotechnology for cancer treatment. Nanotechnology is an use for prevention, diagnosis, and treatment. nanotechnology offers a promise for the targeted delivery of drugs, genes and protein to tumer tissue and therefore alleviating the toxicity of anticancer agent in healthy tissues. Cancer is one of the leading causes of death worldwide. Nanotechnology is one of the most rapidly growing fields in the 21st centuryThese mainly include arrays of nanocantilevers, nanotubes and nanowires for multiplexing detection, multifunctional injectable nanovectors for therapeutics and diagnostics. This article review current nanotechnology platforms for anticancer drug delivery, including polymeric nanoparticles, liposomes, dendrimers, nanoshells, nuclear acid base nanoparticle [DNA, RNA interference (RNAi), and antisense oligonucleotide (ASO) ] The review increases awarnes of advantages in cancer therapy.
Differential spectrophotometric method for estimation and validation of Verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, beeers law limits 525µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamine in simple, precise and economically and it recommended for the routine drug quality analysis investigation.
Nanoparticle of plant extract: A Novel approach for cancer theraproshan telrandhe
Nanotechnology deals regulating matter at dimension of 1-100 nanometers. its use in fundamental physics, biology, chemistry, technology of nanometer scale objects. It also includes how such objects can be used in the areas of computation, sensors, nanostructure materials, biolabeling, biomedical, agricultural, biolabeling , cancer, biotechnology. There are 3 methods for preparation of nanoparticles Physical, chemical &biological. Cancer is as an abnormal growth of cells. It is due to lack of proper regulation in cell cycle. Cancer develops through an accumulation of genetic changes or mutations which could emerge due to different factors like physical, chemical, biological. Currently available cancer chemotherapeutic agents insidiously affect the host cells especially bone marrow, epithelial tissues, reticule-endothelial system and gonads Because of high death rate associated with cancer and the serious side effects of chemotherapy & radiation therapy. Silver NanoParticles as an anticancer agent and they have all turned up positive. Many plant-derived products have been reported to exhibit potent antitumour activity against several rodent and human cancer cell lines. Plants history about use in the treatment of cancer. It is significant that over 60% of currently used anticancer agents are derived, in one way or another, from natural sources (plants, marine organism, and microorganisms)
NOVEL IPQCL AND FPQC TEST FOR OPTHALMIC PREPARATION AS PER IP, BP A...roshan telrandhe
Ophthalmic preparation are the sterile liquid or semisolid preparation meant to installation in to the eyes in the space between eye lids and eye ball .
These product must be sterile and are prepare under the same condition as that of parenteral preparation
Differential spectrophotometric method for estimation and validation of Verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, beeers law limits 525µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamine in simple, precise and economically and it recommended for the routine drug quality analysis investigation.
Formulation and Evalua-tion of Herbal Toothpaste: Compared With Marketed Prep...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability- Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
Formulation and Evaluation of Herbal Toothpaste: Compared with marketed prepa...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability- Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
formulation and evaluation of tooth gel from aloe vera leaves extractroshan telrandhe
The aimed of current research to formulate tooth gel utilizing leaf extract of Aloe vera. In multiple clinical studies, Aloe vera has used in dentistry for wound-healing effect, gingivitis, plaque control and curing oral mucosal lesions. Aloe vera is natural, ancient ingredient. The formulated Aloe vera tooth gel evaluated by physical examination: Colour-yellowish brown, Appearance-Homogeneous, smooth nature, Transparency-translucent and Relative density-10.5, No microbial growth in sample plate, pH7.5, Viscosity-310000cp, Extrudability amount percent-91.33, Spreadability-6.5cm/sec and obsereved good stability. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated aloe vera tooth gel exhibited notable activity with ZOI of 19.5 mm at MIC of 25μg/mL. The outcome from this research evidently signified that the natural plant Aloe vera using to formulate tooth gel may be a new approach to formulate tooth gel economically and minimum side effect than synthetic formulation and good scope in future about dental research in natural remedies.
Nanotechnology for cancer therapy recent developmentsroshan telrandhe
This paper is an overview of advances and prospects in applications of nanotechnology for cancer treatment. Nanotechnology is an use for prevention, diagnosis, and treatment. nanotechnology offers a promise for the targeted delivery of drugs, genes and protein to tumer tissue and therefore alleviating the toxicity of anticancer agent in healthy tissues. Cancer is one of the leading causes of death worldwide. Nanotechnology is one of the most rapidly growing fields in the 21st centuryThese mainly include arrays of nanocantilevers, nanotubes and nanowires for multiplexing detection, multifunctional injectable nanovectors for therapeutics and diagnostics. This article review current nanotechnology platforms for anticancer drug delivery, including polymeric nanoparticles, liposomes, dendrimers, nanoshells, nuclear acid base nanoparticle [DNA, RNA interference (RNAi), and antisense oligonucleotide (ASO) ] The review increases awarnes of advantages in cancer therapy
Differential spectrophotometric method for estimation and validation of verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, Beers law limits 5-25µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamil in simple, precise and economically and it recommended for the routine drug quality analysis investigation
Formulation and evaluation of herbal toothpaste compared with marketed prepar...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability- Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
Formulation and evaluation of herbal toothpaste compared with marketed prepar...roshan telrandhe
The aimed of current research to formulate herbal toothpaste utilizing plant extract like Neem leaves, Guava leaves, Cinnamon bark other ingredient are Camphor, Honey. The plant extract ingredient posses the anti-bacterial. The herbal toothpaste formulated which can satisfy all the required condition to keep the mouth fresh and prevent tooth decay by bacteria. The formulated herbal toothpaste compared with marketed preparation. Physical examination: Colour-greenish brown, smooth in nature, relative density-10.2, pH-8.2, Extrudability-90.37, spredability- Good and stable formulation. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated herbal tooth paste exhibited notable activity with ZOI of 19.7 mm at MIC of 25μg/mL. the outcome of this research herbal toothpaste shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation(Colgate, Dabour Red, Dantkanti) with formulated herbal toothpaste. It has been good scope in future dental research and detal health of public.
Formulation and evaluation of tooth gel from aloe vera leaves extractroshan telrandhe
The aimed of current research to formulate tooth gel utilizing leaf extract of Aloe vera. In multiple clinical studies, Aloe vera has used in dentistry for wound-healing effect, gingivitis, plaque control and curing oral mucosal lesions. Aloe vera is natural, ancient ingredient. The formulated Aloe vera tooth gel evaluated by physical examination: Colour-yellowish brown, Appearance-Homogeneous, smooth nature, Transparency-translucent and Relative density-10.5, No microbial growth in sample plate, pH-7.5, Viscosity-3100cp, Extrudability amount percent-91.33, Spreadability-6.5cm/sec and obsereved good stability. The anti-microbial evaluation against Staphylococcus aureus reveal that formulated aloe vera tooth gel exhibited notable activity with ZOI of 19.5 mm at MIC of 25μg/mL. The outcome from this research evidently signified that the natural plant Aloe vera using to formulate tooth gel may be a new approach to formulate tooth gel economically and minimum side effect than synthetic formulation and good scope in future about dental research in natural remedies.
Nanoparticle of plant extract: A Novel approach for cancer therapyroshan telrandhe
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel Approach For Cancer Treatment. Sponsered By Indian Councial Of Medical Research, Delhi. OrganisedBy KamlaNehru College Of Pharmacy, Butibori, Nagpur441108, Maharastra, India. ON 25 & 26 March 2017
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
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Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activit
1. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activity
1 | P a g e NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
NATIONAL CONFERENCE:
A Phytomedicine: A Novel Approach For Cancer Treatment
Sponsered By
Indian Councial of Medical Research, Delhi.
Organised By
Kamla Nehru College of Pharmacy, Butibori, Nagpur 441108, Maharastra, India.
ON 25 & 26 March 2017
Conference Paper
Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti
- Staphylococcus aureus activity
Roshan Telrandhe*
Kamla Nehru College Of Pharmacy, Butibori, Nagpur 441108, Maharastra, India
Presented on 26 March 2017
Abstract
The current research aimed at fabricating plant extract mediated biosynthesized silver
nanoparticles (AgNPs) utilizing thorn extract of Bombax ceiba (TEBC). The synthesized AgNPs
was characterized by UV spectroscopy where the surface plasmonic resonance peak (SPR) was
located at 222 nm. The scanning electron microscopy (SEM) studies demonstrated that the
morphology of fabricated nanomaterials was primarily cylindrical of average size of 20-30 nm
with some spindles of size >50 nm. The anti-microbial evaluation against Staphylococcus aureus
revealed that AgNPs exhibited notable activity with ZOI of 27.2 mm at MIC of 25 μg/mL. The
outcome of this research evidently signified that the biofabricated AgNPs using TEBC may be a
new greener approach or technology to formulate anti-bacterial nanodrugs in future.
Keywords: Bombax ceiba, extract, silver, nanopar
ticle, biosynthesis, green synthesis.
2. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activity
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INTRODUCTION
Nanotechnology remains the most
promising, emerging, and interesting section
of research in 21st century owing to its
diverse applications with modified physico-
chemical attributes as compared to the bulk
material1
. In the last few decades, the
application of nanomaterials in the area of
biology and medicine has revolutionized the
field of drug delivery, theranostics, imaging,
diagnosis, wound healing, and medical
devices with miscellaneous properties2
.
Nanomedicine is the sub-branch expanding
where nanocomposite products and
technologies of 1-100 nm range are of great
importance.
Metallic nanoparticles are foremost
preferred in material chemistry owing to
their physicochemical characteristics. Silver
nanoparticles have citadel importance in the
present era and are extensively used in
pharmaceutical sciences, drug delivery,
biomedical sciences, catalysis, etc3
. They
can be conjugated with various functional
biomolecules and can be used to target
numerous diseases. The role of silver as
anti-bacterials is known for centuries and is
seen in museums as daily use products
where they are majorly composed4
. These
nanomaterials possess high ratio of surface
area to mass and have amazing bactericidal
properties by diverse mechanism of actions5
.
The anti-microbial activity is majorly
believed to be the cumulative toxic effect of
the product which is exerted by metallic
nature causing detrimental effects on normal
cellular proliferation and protein functions6
.
Since late 90s, the silver nanoparticles were
synthesized by chemical, physical, or
photochemical methods7
. As cost, particle
size and distribution, scalability, and
dispersion remained the crucial aspects,
chemical method for the nanoparticle
fabrication was usually applied8
. However,
in the due course of time, the adverse toxic
effect to the environment compelled the
producers to opt for safer, environment
friendly, green technique for the
fabrication9
. In recent times, this approach
received substantial notice owing to lowest
production cost, safety, environmentally
friendly aspects, and simplest procedure for
the synthesis. The plant biomass serves as
reductant and capping agents, where an
aqueous solution of metallic salt reacts to
form nanoparticles rapidly10
.
Bombax ceiba or silk cotton tree, belonging
to the family of Bombacaceae is a large
deciduous tree found throughout India. It is
well known for its medicinal properties
ethnopharmacologically in the society for
centuries and in traditional systems like
Ayurveda, Siddha, and Unani. The plant
extract is known for pharmacological
activities such as anti-inflammatory, anti-
hypertensive, antimicrobial, analgesic, anti-
angiogenic, anti-viral, etc11
. Recently,
Bombax ceiba leaf extract has been
employed to synthesize silver nanoparticles.
The thorn extract of Bombax ceiba (TEBC)
is reported to have potent anti-oxidant, anti-
microbial, antiinflammatory activities12
.
However, there is no report of exploiting the
thorn extract to fabricate size controlled and
uniform silver nanoparticles. The present
research aimed at synthesizing silver
3. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activity
3 | P a g e NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
nanoparticles utilizing TEBC as a green
approach to provide the foundation for
commercial production of nanomaterial with
environmental friendly attributes.
MATERIALS AND METHODS
Chemicals
Silver nitrate was purchased Sigma-Aldrich
(Germany). Other analytical grade reagents
and consumables were procured from
HiMedia (India). Double distilled water
apparatus (Borosil®, India) was used for the
experiment.
Collection
The fruits of Bombax ceiba were collected
from the tree present at the medicinal garden
campus of Kamla Nehru College of
Pharmacy situated in the Butibori area of
Nagpur City in Maharashtra State of India.
The plant was identified and authenticated
by Dr. Dongarwar, Department of Botany,
RTM Nagpur University, Nagpur,
Maharashtra.
Extraction
The thorns were collected from the tree,
dried in the shade for a specified period, and
powdered suitably. The dried powder,
divided into multiple smaller amounts, was
subjected to continuous hot Soxhlet
extraction with 50 mL distilled water and 50
mL alcohol (ethanol 90%) in equal ratio at a
temperature of 55-65°C during 32 cycles.
The solvent was removed under reduced
pressure and
controlled temperature using a rotary
vacuum evaporator. The hydroalcoholic
thorn extract of Bombax ceiba (TEBC) yield
was found to be 11.8% w/w.
Preparation of silver nitrate solution
For the experiment, 1mM aqueous solution
of silver nitrate (AgNO3) was prepared by
dissolving 0.01698g of AgNO3 in 100 mL
of double distilled water.
Synthesis of silver nanoparticles
For the preparation of silver nanoparticles, 1
mL of TEBC was taken and in that 9 mL of
1mM aqueous solution of silver nitrate was
added. The content was incubated at 37°C
for 24 hours. The color change was checked
frequently, which is an indicator of silver
nanoparticles formation13.
Characterization of silver nanoparticles
UV-Vis spectroscopic study
The silver nitrate solution and reduction of
pure silver ions was monitored after 24
hours on a UVVis spectrophotometer
(Shimadzu® UV-1800, Kyoto double beam
model) in 200-800 nm wavelength range.
The samples were prepared in small aliquots
with distilled water14.
4. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti
4 | P a g e NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
Figure 1. UV spectra of synthesized
nanoparticles.
Scanning electron microscopic study
The morphology of the fabricated silver
nanoparticles was studied using scanning
electron microscopy (SEM) technique
(JOEL-JSM 6390 SEM machine). For the
analysis, the samples were prepared by
sprinkling the nanomaterial on the double
tape wedged to the aluminium stub.
Subsequently, the sample containi
was then placed in the SEM chamber and
scanned randomly at the acceleration
voltage of 10 kV. The photomicrographs
were taken and the results were recorded15.
Figure 2. SEM micropictograph of
fabricated silver nanoparticles.
extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activity
NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
UV spectra of synthesized
Scanning electron microscopic study
The morphology of the fabricated silver
was studied using scanning
electron microscopy (SEM) technique
JSM 6390 SEM machine). For the
analysis, the samples were prepared by
sprinkling the nanomaterial on the double
tape wedged to the aluminium stub.
Subsequently, the sample containing stub
SEM chamber and
scanned randomly at the acceleration
voltage of 10 kV. The photomicrographs
were taken and the results were recorded15.
SEM micropictograph of
Anti-bacterial activity
The in-vitro anti-bacterial activity of silver
nanoparticles was performed by disc
diffusion method in a triplicate manner
using Muller Hinton Agar medium against a
pathogenic bacterial strain Staphylococcus
aureus (S. aureus, MTCC 3160). S. aureus
was initially cultured in nutrient broth and
incubated at 37°C for 24 hr and then the
cultured cells were tend to multiply in the
Muller Hinton agar plates. Afterward, the
silver nanoparticle containing discs were
placed over the bacterial plates and
incubated at 37°C for 24 hrs, comparing
ciprofloxacin as the positive control). The
diameter of the zone of inhibition (ZOI) was
measured in millimeters (mm).
The minimum inhibitory concentration
(MIC) is the smallest concentration in which
the compound displays no visible microbial
growth. It was determined by agar streak
dilution method in triplicate manner. The
protocol involve formation of microbial
suspension (~105 CFU/mL), application to
the petridish with serial dilution and
incubation of petridish at 37±1ºC
value was determined and the average was
taken16.
RESULTS AND DISCUSSION
It was observed that upon addition of the
extract into the flask containing the aqueous
silver nitrate solution, the color of the
medium changed to dark brown within 2
min. This reflected the conversion of ionic
form of silver into the elemental form. The
formation of the silver nanoparticles was
further
Staphylococcus aureus activity
NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
bacterial activity of silver
nanoparticles was performed by disc
diffusion method in a triplicate manner
using Muller Hinton Agar medium against a
pathogenic bacterial strain Staphylococcus
aureus (S. aureus, MTCC 3160). S. aureus
nitially cultured in nutrient broth and
incubated at 37°C for 24 hr and then the
cultured cells were tend to multiply in the
Muller Hinton agar plates. Afterward, the
silver nanoparticle containing discs were
placed over the bacterial plates and
at 37°C for 24 hrs, comparing
ciprofloxacin as the positive control). The
diameter of the zone of inhibition (ZOI) was
measured in millimeters (mm).
The minimum inhibitory concentration
(MIC) is the smallest concentration in which
o visible microbial
growth. It was determined by agar streak
dilution method in triplicate manner. The
protocol involve formation of microbial
suspension (~105 CFU/mL), application to
the petridish with serial dilution and
incubation of petridish at 37±1ºC. The MIC
value was determined and the average was
RESULTS AND DISCUSSION
It was observed that upon addition of the
extract into the flask containing the aqueous
silver nitrate solution, the color of the
medium changed to dark brown within 2
. This reflected the conversion of ionic
form of silver into the elemental form. The
formation of the silver nanoparticles was
5. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti - Staphylococcus aureus activity
5 | P a g e NATIONAL CONFERENCE: A Phytomedicine: A Novel Approach For Cancer Treatment
confirmed by two important analytical
techniques: UV spectroscopy and scanning
electron microscopy.
UV spectroscopic study
The nanoparticle sample displayed the
absorbance peak of about 222 nm. The
peaks arise due to the absorption
phenomenon of metallic silver nanoparticles
as a result of the Surface Plasmon
Resonance (SPR). The absorption peak near
to 200 nm represents the interaction of
numerous organic molecules with the silver
ions in the solution whereas the disturbances
observed at nearly 300 nm probably
indicated that capping occured after the
reduction of the silver nanoparticles. The
above occurrence suggested the plausible
mechanism(s) for the reduction of the silver
ions into their elemental form. Figure 1
describes the UV spectra recorded from the
aqueous solution of silver nitrate with
TEBC.
Scanning electron microscopic study
The morphology of the fabricated
nanoparticles was identified to be primarily
cylindrical. A few nanoparticles were rod
shaped and long. However, no round or
circular geometry was observed. Although,
the disparity in terms of size, shape,
morphology, etc. are usually seen in
nanomaterials synthesized by plant extracts.
It was noticed that the nanoparticles edges
were much lighter than that of the centre
which indicated that the proteins capped the
silver nanoparticles. The SEM images
demonstrated average size of about 20-30
nm with some spindles of size >50 nm
(Figure 2).
Anti-microbial activity
The silver nanoparticles exhibited fairly
good anti - S. aureus activity as compared to
the standard drug ciprofloxacin. The
nanomaterial exhibited an impressive ZOI of
27.2 mm at MIC of 25 μg/mL whereas
ciprofloxacin exhibited 32.6 mm ZOI at
MIC of 6.25 μg/mL. Therefore, it may be
concluded that the fabricated nanomaterial
have potential to exhibit antimicrobial
activity.
CONCLUSION
The research concluded that TEBC
demonstrated the biofabrication of AgNPs
of average size 20-30 nm. The
characterization of AgNPs by sophisticated
analytical tools showed that the
nanomaterials were formed as desired. The
anti-microbial evaluation against
Staphylococcus aureus revealed that AgNPs
exhibited notable activity with ZOI of 27.2
mm at MIC of 25 μg/mL. The outcome of
this research evidently signified that the
biofabricated AgNPs using TEBC may be a
new greener approach or technology to
formulate anti-bacterial nanodrugs in future.
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