Method to expose rats to ozone-updated2014

Exposing Rats to Ozone – Method Setup
Update 2014
There are other ways to do this much cheaper, but this would put together since this what was freely
available at the time. Today I would probably use an Arduino or some other cheap datalogger with
simple relay capability. There are too many vendors to list.
For ozone monitoring, the 2B Technologies Model 202 Ozone Monitor would do fine. It is EPA
approved as well. It has the capability of built in storage card and has an analog output.
It might also be a good idea to have a small fan and some heating strips to circulate the air and
minimize moisture from the rats' breathing. It is also good to minimize any moisture to prevent damage
to the ozone monitor.
It was never necessary, but it would have been neat to have swapped the potentiometer control for
ozone level with an electronic one. Otherwise, it is a test and go until it is right at first.
Make sure generator and ozone monitor are sized appropriately to the concentrations and size of the
enclose area.
A Crystal Air (or the slightly bigger model)
http://www.ozone.ca/products/DC-PRO-200.htm
Ozone Monitor
http://www.twobtech.com/model_202.htm
Crystal Air
http://www.ozone.ca/products/DC-PRO-200.htm

1

Purpose: This document explains the basics of setting up an experimental system for exposing mice or
rats to high concentrations of ozone. Most of the components may be available over the internet from
lab suppliers or other vendors.
The setup for exposing the rats to ozone consists of a closed chamber, a Dasibi 1008 series ozone
monitor, a Zeno 3200 datalogger from Coastal Environmental Systems, a dehumidifier for the chamber,
a couple of heating strips for regulating temperature/humidity in the chamber, and a Crystal Air Model
450 ozone generator.
The heating strips, dehumidifier, and ozone monitor need to operate constantly. Air is fed into the
chamber from the laboratory compressed air supply. A sampling tube is inside the chamber and attaches
to the ozone monitor. The ozone monitor has an output voltage that is fed into a channel on the Zeno
3200 datalogger. The datalogger records the ozone level in one minute averages and activates a 12V
relay that controls a standard 110V outlet to turn the ozone generator on or off. The ozone level is
programmed into the datalogger and the datalogger records the ozone levels and determines when the
relay should run the ozone generator on or off with some simple if/then combinations that are part of
the Zeno 3200's operating system. A desktop or laptop computer is available only to access the
datalogger with Window's Hyperterminal software to download the data and program the datalogger.
Materials and Equipment
Glove Box: This chamber also has power strip built-in. One power strip or electrical cord will have to
be committed for turning the ozone generator on and off. The heating strips and dehumidifier should be
left on all the time. This will probably require another power strip. It will also be important to have
constant supply of lab compressed air flowing into the chamber so the animals do not suffocate.

2

Dasibi 1008 AH Ozone Monitor

This particular model and brand of ozone monitor is no longer made. Other models can be used. Make
sure to keep moisture out of these monitors. Some models do not tolerate condensation too well and
one could end up with some expensive repairs.
Zeno 3200 Datalogger

http://www.coastalenvironmental.com/
Coastal Environmental Systems, Inc.
820 First Avenue South
Seattle, WA 98134
Phone:206-682-6048
800-488-8291
Fax: 206-682-5658
This model has a numerous channels for recording electrical signals from ozone monitors to ph meters
or wind speed or thermocouples. It also has some general programming capability so electrical relays
can be activated at certain times or when a specific value has been reached by a recording instrument. It
functions independently of a desktop or laptop computer.

3

12V Relay

Radioshack
12VDC/10A DPDT Plug-in Relay
Model: 275-218 | Catalog #: 275-218
Wiring Diagrams/Schematics
Materials: 12V Relay, some small gauge wire of a few inches or more (used to wire the relay and
ozone monitor voltage output to datalogger), an electrical cord, a standard outlet with 2 plugs, a box
and cover to house the outlet and relay.
The standard electrical cord of 12 or 14 gauge wire will typically have a black, white, and green. One
can also cut off the end of an extra computer cord. Solder the black wire and white wire to the prongs
as indicated on the relay. Wire the green ground wire directly to the connection on the plug. Solder a n
inch or two from the relay and strip the ends of the wire to connect the outlet to the relay. Most plugs
usually have a copper and silver screw and a ground connection; wire the black to copper and the white
to silver. This outlet will be used to turn the ozone generator on and off.
Wire the relay to the 12V connections on the datalogger; wire one to Channel A, B, or C and the other
to a ground (any of the channels will work, just make sure to modify programming accordingly). Wire
the the output of the ozone monitor to the channel on the datalogger; wire the (+) to the input channel
and the (-) to a ground connection on the datalogger terminals.

4

Zeno Program
Follow this if inputting the setting manually. The program to upload is at the end of this document. This
is a crude control system, so it may be necessary to adjust the values plus or minus at the desired point.
For example, if 800 ppb is desired, the program might be set to 801 or 805 ppb before cutting off the
ozone generator. Keep in mind that the datalogger is an electronic device and it will always DO WHAT
YOU TELL IT TO DO not what you want it to do.
* Zeno 3200 System Configuration Menu File
* Program Version And Date: ZENO-3200 using ZENOSOFT V2.02 Sep 10 2002 11:29:41 CS
B97B
* (C)opyright 1995-2002, Coastal Environmental Systems, Seattle, WA, USA.
* Menu Setup File Date And Time: 07/07/25 16:09:41
COMMUNICATIONS MENU
(Cn/m) Change Item n To Value m
(M)
Modem Menu
(P)
Power Control Menu
(G)
GOES Menu
(A)
ARGOS Menu
(D)
Digital Control Menu
Item
Item
Item
Item
Item
Item
SYSTEM
(Cn/m)
(S)
(T)
(V)
(K)
(B)

1:
2:
3:
4:
5:
6:

(COM1
(COM2
(COM3
(COM1
(COM2
(COM3

Baud
Baud
Baud
Port
Port
Port

SAMPLE
(Cn/m)
(E)
(U)

1:
2:
3:
4:
5:
6:
7:
8:
9:

0
0
1
1
0
0
0
<ESC>

60

(I)
(E)
(U)
(Q)
(H)

Contact Information
Save Parameters To EEPROM
User Menu
Quit
Help

(Primary Unit/Experiment ID)
(Secondary Unit/Experiment ID)
(Data Dump Format)
(Real Time Output Format)
(Add Compass To Vane)
(Compass Offset)
(Barometer Elevation)
(Bad Sensor Value Replace)
(Passthrough Mode Escape Character)

PERIOD MENU
Change Item n To Value m
User Menu
1:

Repeater Menu
Terminal Mode On COM Port n
User Menu
Quit
Help

Rate)
Rate)
Rate)
Type)
Type)
Type)

FUNCTIONS MENU
Change Item n To Value m
System Date And Time
Calibrate Internal Temperature
Program Version
Constants Menu
BIT Names Menu

Item
Item
Item
Item
Item
Item
Item
Item
Item

Item

9600
9600
9600
RS232
RS232
RS232

(R)
(Tn)
(E)
(U)
(Q)
(H)

(Q) Quit
(H) Help

(Sample Interval Time)

5

Item
Item

2:
3:

30
0

(Sample Duration Time)
(Sample Time Offset)

MODEM MENU
(E)
(S)
Speech Interface Menu
Item 1:
Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:
Item 11:

0
NONE
5
0
NONE
NONE
NONE
NONE
NONE
0
0

(System Modem Type)
(Modem COM Port)
(Modem Retry Period)
(Modem Hangup Delay)
(Modem Telephone Number 1)
(Modem Initialization String)
(Modem Switched Voltage)
(Modem Ring Detect Channel)

POWER CONTROL MENU
(E)
(U)
User Menu
Item
Item
Item
Item
Item

1:
2:
3:
4:
5:

100
0
0
0
0

(Q) Quit
(H) Help

(COM1 Push-To-Talk Setup Time)
(COM1 Switched Power Code)
(Response Delay Time (milliseconds)

GOES MENU
(D)
Run GOES Diagnostics
(R)
Reset GOES Errors
(I)
Initialize GOES
Item 1:
Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:
Item 11:

(U) User Menu
(Q) Quit
(H) Help

03001190
151
00:03:00:00
00:00:00
1
SHORT
151
00:00:00
00:05:00
1200
NONE

(E)
(U)
(Q)
(H)

User Menu
Quit
Help

(Data Collection Platform Address)
(Self-Timed Transmit Channel Number)
(Self-Timed Transmission Interval)
(Self-Timed Transmission Offset)
(Transmission Window Length)
(Satellite Link Parameter: Preamble)
(Random Transmit Channel Number)
(Random Transmission Interval)
(Random Disable Time)
(GOES Bit Rate)
(GOES Interleaver)

DIGITAL CONTROL MENU
(E)
(U)
User Menu

(Q) Quit
(H) Help

6

Item
Item
Item
Item

1:
2:
3:
4:

000:00:00
000:00:00
03/07/29 15:00:00
0

(Power On Interval)
(Power On Duration)
(Start of First Power On)
(Control Channel)

REPEATER MENU
(R)
Reset All Repeater Values
(E)
Item 1:
Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:
Item 11:

(U) User Menu
(Q) Quit
(H) Help

Repeater Tranmit Delay
Network Source Address
Alternative Source Address
Remote ZENO Address #1

0 msec
-1
-1
-1
-1
-1
-1
-1
-1
-1
-1

ARGOS MENU
(E)
(D)
Display ARGOS Message
Item
Item
Item
Item
Item
Item

1:
2:
3:
4:
5:
6:

42
1
10
0
NO
NO

(U) User Menu
(Q) Quit
(H) Help

(Tranmission Repeat Interval, seconds)
(Transmission Repeat Count)
(Radio Push-To-Talk Delay Time, milliseconds)
(Radio Push-To-Talk Digital Port)
(Use Hour/Min Time Stamp in Message)
(Use Checksum in Final Message Byte)

CONSTANTS MENU
(Cn/m) Change Constant n to Value m
(P)
Previous Page of Constants
(N)
Next Page of Constants
(X)
Erase All Constants
Constants 1 through 20
Constant 1: 0
Constant 2: 100
Constant 3: 0
Constant 4: 0
Constant 5: 0
Constant 6: 0
Constant 7: 0
Constant 8: 0
Constant 9: 0
Constant 10: 0

Constant
Constant
Constant
Constant
Constant
Constant
Constant
Constant
Constant
Constant

(E)
(U)
(Q)
(H)
11:
12:
13:
14:
15:
16:
17:
18:
19:
20:

SPEECH INTERFACE MENU

7

0
0
0
0
0
0
0
0
0
0

User Menu
Quit
Help

(E)
(U)
User Menu
Item
Item
Item
Item

1:
2:
3:
4:

NO
0
0
0

(Speech
(Speech
(Speech
(Speech

Interface Installed)
Activity Channel)
Tone Detect Channel)
Switched Voltage)

BIT NAMES MENU
(Cn/m) Change Bit n to Value m
(E)
(U)
User Menu
BIT
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit

(Q) Quit
(H) Help

Names 1 through 32
1: ZENO-3200-Reset
2: Real-Time-Clock-Suspect
3: Logging-Memory-Initialized
4: Serial-Sensor-COM-Failure
5: EEPROM-Suspect
6: 18-Bit-ADC-Suspect
7: 12-Bit-ADC-Suspect
8: Temperature-Clock-Adjustment
9:
10:
11:
12:
13:
14:
15:
16:

(Q) Quit
(H) Help

Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit
Bit

BACK DOOR MENU
(F)
Calculate Free Heap Memory
(A)
Auto-Calibrate Compass
(I)
Initialize Compass
(R)
Reset Parameters To Defaults
(Y)
Configuration Heap Usage
Item 1:
Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:
Item 11:

16777
0
60
18
VOLTS
NO
32767.69
0
1.0000
0
500

17:
18:
19:
20:
21:
22:
23:
24:
25:
26:
27:
28:
29:
30:
31:
32:
(G)
(X)
(E)
(W)
(U)
(H)

Reinitialize Flash Memory
Display Stack Usage
Harware Test Menu
User Menu
Help

(Processor Clock Speed)
(RAM/ROM Wait States)
(50/60 Hz Rejection For 18-bit ADC)
(13/18 Bit Operation Of 18-bit ADC)
(A To D Conversion Results)
(Expert Menu Mode)
(Real-time Clock Crystal Frequency At 25 Degrees C)
(Speed vs. Noise Tradeoffs For 18-bit ADC, Factory Only)
(12-bit ADC Correction Factor)
(Zero Offset Period in Seconds)
(Low Freq. Sensor Timeout in msec)

SENSOR MENU

(Jn) Jump To Record n

8

(A)
(B)
(X)
(C)
(V)
(Sn/m)

Insert After This Record
Insert Before This Record
Cut Record to Clipboard
Copy Record To Clipboard
Paste Record From Clipboard
Search Item n for Value m

(N)
(P)
(XA)
(Z)
(H)

Go To Next Record
Go To Previous Record
Delete ALL Records
Zeno Program Menu
Help

Sensor Items for Record 1 of 3:
Item 1: Sensor Type Code
Item 2: Sensor Name
Item 3: Sensor Input Channel
Item 4: Analog Channel Gain
Item 5: Analog Channel Attenuation
Item 6: Switched Power Code
Item 7: Sensor Excitation Voltage Code
Item 8: Switched Excitation Return
Item 9: Switched Power Warmup Time
Item 10: Sensor Sample Count
Item 11: Maximum Sensor Readings
Item 12: Sensor Timing Loop
Item 13: Conversion Coefficient A
Item 14: Conversion Coefficient B
Item 15: Conversion Coefficient C
Item 17: No. of Additional 15-msec Delays

3 (18-bit Differential A to D)
pHST
7
1
1
3 (12V SW'D A)
0 (NO EXCITATION VOLTAGE)
0
3
1
0
2 (1.0 seconds)
0
40
0
0

Item 2: Sensor Name
Item 9: Switched Power Warmup Time
Item 10: Sensor Sample Count
Item 11: Maximum Sensor Readings
Item 12: Sensor Timing Loop
Item 13: Conversion Coefficient A
Item 14: Conversion Coefficient B
Item 15: Conversion Coefficient C
Item 17: No. of Additional 15-msec Delays

pHMT
3
1
1
3 (12V SW'D A)
0
3
1
0
2 (1.0 seconds)
0
40
0
0

Item 2: Sensor Name

cond
5
1
1
3 (12V SW'D A)
0

9

Item
Item
Item
Item
Item
Item
Item
Item

9:
10:
11:
12:
13:
14:
15:
17:

Switched Power Warmup Time
Sensor Sample Count
Maximum Sensor Readings
Sensor Timing Loop
Conversion Coefficient A
Conversion Coefficient B
Conversion Coefficient C
No. of Additional 15-msec Delays

PROCESS MENU
(A)
(B)
(X)
(C)
(V)
(Sn/m) Search Item n for Value m

3
1
0
2 (1.0 seconds)
0
40
0
0

(Jn)
(N)
(P)
(XA)
(Z)
(H)

Jump To Record n
Go To Next Record
Delete ALL Records
Zeno Program Menu
Help

Process Items for Record 1 of 5:
Item 1: Process Category
Item 2: Process Number
Item 3: User-defined Process Name
Item 4: Input for Data (X)
Item 5: A2 Coefficient

5 : Arithmetic
8 : Quadratic Process
pHST
S1 : pHST
0
1.113
-6.148


5 : Arithmetic
pHMT
S2 : pHMT
0
0.98
-4.522


5 : Arithmetic
Cond
S3 : cond
0
694.5
-2777.8


3

10

: Alarm

Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:

Process Number
User-defined Process Name
Input for Data
Alarm Setpoint
Alarm Reset
BIT Flag Number (1 - 31)
Digital Output Channel 1
Digital Output Channel 2
Switched Voltage

3 : Alarm Process
pHch3
S2 : pHMT
12
13.5
0
0
0
4

Item 4: Input for Data
Item 5: Alarm Setpoint
Item 6: Alarm Reset
Item 7: BIT Flag Number (1 - 31)
Item 8: Digital Output Channel 1
Item 9: Digital Output Channel 2
Item 10: Switched Voltage

3 : Alarm
3 : Alarm Process
cond
S3 : cond
6.5
6.4995
0
0
0
5

DATA OUTPUT MENU
(A)
(B)
(X)
(C)
(V)
(Sn/m) Search Item n for Value m

(Jn)
(N)
(P)
(XA)
(Z)
(H)

Jump To Record n
Go To Next Record
Delete ALL Records
Zeno Program Menu
Help

Data Items for Record 1 of 5:
Item 1: Field Type Code
Item 2: Output Message(s)
Item 3: Field Name
Item 4: Input Record
Item 5: Field Decimal Places
Item 6: Field Width
Item 7: Data Storage Class Code

9 : Transmit and Log Data Field
1
pHmAST
S1 : pHST
1
6
8 : 4-byte floating point

Item 3: Field Name
Item 6: Field Width

1
pHST
P1.1 : pHST
1
6

11

Item 3: Field Name
Item 6: Field Width

1
condST
P3.1 : Cond
1
6

Item 3: Field Name
Item 6: Field Width

1
pHmAMT
S2 : pHMT
1
6

Item 3: Field Name
Item 6: Field Width

1
pHMT
P2.1 : pHMT
1
6

GENERAL SERIAL SCRIPT MENU
(Cn/m) Change Line n to m
(In)
Insert Line n
(Rn)
Remove Line n
(En)
Erase Line n
(L)
Delete All Lines
(A)
Insert After this Script
(B)
Insert Before this Script

(X)
(Jn)
(N)
(P)
(XA)
(Z)
(H)

Delete this Script
Jump to Script n
Go to Next Script
Go to Previous Script
Delete All Scripts
Zeno Program Menu
Help

Script Lines for Script 1 of 1
Line 1: NO_COMMAND
SENSOR TIMING LOOP MENU
(Z)
Zeno Program Menu
Item
Item
Item
Item

1:
2:
3:
4:

0.5
1.0
1.5
2.0

(Timing
(Timing
(Timing
(Timing

Loop
Loop
Loop
Loop

OUTPUT MESSAGE TIMING MENU
(Z)
Zeno Program Menu

(H) Help
#1
#2
#3
#4

Period)
Period)
Period)
Period)
(H) Help

12

Item 1:
Item 2:
Item 3:
Item 4:
Item 5:
Item 6:
Item 7:
Item 8:
Item 9:
Item 10:

COM3
COM3
COM3
COM3
0.0
0.0
0.0
0.0
0
0

(Data Output Message #1 COM Port)
(Data Output Message #1 Period)
(Record Counter Starting Value)
(Record Counter Reset Value)

MEMORY MANAGEMENT MENU
(Z)
Zeno Program Menu

(H) Help

Item 1: 0
(COM1 GSI Memory Size in Bytes)
Item 2: 0
Item 3: 0
* !!SYSTEM TRANSFER COMPLETE.
* Turn Off File Capture Now.
* Enter Any Key To Continue.
EOF

Copy this to a text editor like Notepad and upload the file to the Zeno 3200. Make sure the top
line is the beginning of the file with no extra spaces:
* Zeno 3200 System Setup File
* Program Version And Date: ZENO-3200 using ZENOSOFT V2.02 Sep 10 2002 11:29:41 CS
B97B
* (C)opyright 1995-2002, Coastal Environmental Systems, Seattle, WA, USA.
* Setup File Date And Time: 07/07/25 16:09:19
PARAM1 60 0 30 2 3 4 0 0 9600 9600
PARAM2 9600 0 0 0 0 1 1 0 0 0
PARAM3 16777 0 60 18 0 0 0 0 2 2
PARAM4 2 2 0 0 0 3276769 0 -1 5 0
PARAM5 3 0 0 0 100 0 0 0 0 0
PARAM6 0 0 0 1059577200 50336144 151 196608 0 1 0
PARAM7 151 0 1280 0 10000 -1 -1 0 10 1
PARAM8 42 0 0 0 0 0 0 0 0 0
PARAM9 0 0 0 0 0 0 5 0 0 0
PARAM10 500 0 0 0 1200 -1 27
PARAM11 "NONE" "NONE" "NONE" "NONE" "NONE" "NONE" "" "ZENO" "" "NONE"
PARAM12 "" "ZENO-3200-Reset" "Real-Time-Clock-Suspect" "Logging-Memory-Initialized"
"Serial-Sensor-COM-Failure" "EEPROM-Suspect" "18-Bit-ADC-Suspect" "12-Bit-ADCSuspect" "Temperature-Clock-Adjustment" ""
PARAM13 "" "" "" "" "" "" "" "" "" ""
PARAM14 "" "" "" "" "" "" "" "" "" ""
PARAM15 "" "" ""
REPEAT1 -1 -1 -1 -1 -1 -1 -1 -1
CONSTANT1 0 100 0 0 0 0 0 0 0 0
CONSTANT2 0 0 0 0 0 0 0 0 0 0
GSI 1 NO_COMMAND
SENSOR 3 "pHST" 7 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1

13

SENSOR 3 "pHMT" 3 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1
SENSOR 3 "cond" 5 0 0 3 0 0 3 1 0 2 0 40 0 0 0 0 0 0 0 0 0 0 S0.1
PROCESS 5 8 "pHST" S1.1 0 1.113 -6.148
PROCESS 5 8 "pHMT" S2.1 0 0.98 -4.522
PROCESS 5 8 "Cond" S3.1 0 694.5 -2777.8
PROCESS 3 3 "pHch3" S2.1 12 13.5 0 0 0 4
PROCESS 3 3 "cond" S3.1 6.5 6.4995 0 0 0 5
DATA 9 1 "pHmAST" S1.1 1 6 8 P1.1 P1.1 P1.1
DATA 9 1 "pHST" P1.1 1 6 8 P1.1 P1.1 P1.1
DATA 9 1 "condST" P3.1 1 6 8 P1.1 P1.1 P1.1
DATA 9 1 "pHmAMT" S2.1 1 6 8 P1.1 P1.1 P1.1
DATA 9 1 "pHMT" P2.1 1 6 8 P1.1 P1.1 P1.1
* !!SYSTEM TRANSFER COMPLETE.
* Turn Off File Capture Now.
* Enter Any Key To Continue.
EOF

14

110V
AC

Relay and outlet wired
together inside box.
Control wires go to
chosen 12V channel on
datalogger. The ozone
value is read from the
ozone monitor to turn
the ozone generator on
and off.

Ozone
Monitor

Ozone
Generator

Fan. Circulates air in
chamber and shows
that circuit is turned
on.

(+) and (-) for
output voltage
of monitor.
Dehumidifier
and heat
strips inside
H
e chamber.
a
t
S
t
r
i
p

15

Method to expose rats to ozone-updated2014

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