This document discusses screening methods for anticancer drugs. It begins with an introduction to cancer and the importance of screening methods to find agents that can target solid tumors. It then covers the history of anticancer drug screening, including early small-scale studies on tumor growth. Various definitions of cancer and types of cancer are provided. The document focuses on in vitro and in vivo screening methods, such as dye exclusion assays, enzyme assays, and methods using animal models like inducing tumors in mice. It concludes that understanding these screening methods can help in developing novel anticancer drugs with improved selectivity and specificity.
In vitro methods of screening of anticancer agentsNikitaSavita
Cancer- It is the leading cause of mortality. Cancer is the disease which is categorized by abnormal cell growth with the dormant to spread to other parts of body.
The versatile instrument is used to isolate unknown compounds from a HPTLC/TLC plate and transfer them into a mass spectrometer for identification or structure elucidation.
In vitro methods of screening of anticancer agentsNikitaSavita
Cancer- It is the leading cause of mortality. Cancer is the disease which is categorized by abnormal cell growth with the dormant to spread to other parts of body.
The versatile instrument is used to isolate unknown compounds from a HPTLC/TLC plate and transfer them into a mass spectrometer for identification or structure elucidation.
Insulin Immunoassay Insights: Unraveling the Biochemical ThreadsAshish Gadage
"Dive into the intricate world of insulin with our Immunoassay Insights! Uncover the secrets of biochemical interactions in a journey through precision and discovery. 🧪🔍 #InsulinAssay #Biochemistry"
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
In gas chromatography, the sample is first converted to the vapor state or volatile compound is
used which are injected into heated port along with a carrier gas. Analyte in the vapor state
distributes between the stationary phase and the carrier gas. As the analyte elute from column
signal is displayed in the form of chromatogram. Measurements of GC are rapid and convenient.
Retention times are used for qualitative identification. Peak areas are used for quantitative
measurements.
Mass spectroscopy
1.Introduction
2. Principle
3. Theory
4. Instrumentation
5. Different types of Ionization
6. FAB
7. MALDI
8. APCI
9. ESI
10.Quardapole
Role of cancer stem cells in cancer therapyniper hyd
Cancer is one of the leading cause of death, till to date there is no perfect treatment due to its stem cell relapse which is not responding to conventional therapies like chemo, radio, surgery. so we should target the cancer stem cell with novel targets like nano, and immunotherapies.
Insulin Immunoassay Insights: Unraveling the Biochemical ThreadsAshish Gadage
"Dive into the intricate world of insulin with our Immunoassay Insights! Uncover the secrets of biochemical interactions in a journey through precision and discovery. 🧪🔍 #InsulinAssay #Biochemistry"
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
In gas chromatography, the sample is first converted to the vapor state or volatile compound is
used which are injected into heated port along with a carrier gas. Analyte in the vapor state
distributes between the stationary phase and the carrier gas. As the analyte elute from column
signal is displayed in the form of chromatogram. Measurements of GC are rapid and convenient.
Retention times are used for qualitative identification. Peak areas are used for quantitative
measurements.
Mass spectroscopy
1.Introduction
2. Principle
3. Theory
4. Instrumentation
5. Different types of Ionization
6. FAB
7. MALDI
8. APCI
9. ESI
10.Quardapole
Role of cancer stem cells in cancer therapyniper hyd
Cancer is one of the leading cause of death, till to date there is no perfect treatment due to its stem cell relapse which is not responding to conventional therapies like chemo, radio, surgery. so we should target the cancer stem cell with novel targets like nano, and immunotherapies.
Key achievements in cancer research at Cold Spring Harbor Laboratory: May 201...cshlnews
May is National Cancer Research Month. In recognition, here's a glimpse of key achievements in cancer research over the last 12 months (May 2010 - May 2011) at Cold Spring Harbor Laboratory, which has been an NCI-designated Cancer Center since 1987.
The different types of available drug delivery systems in cancer. Present scenario and what are top drug and future prospect.
The ways how nanoparticle helping as drug delivery carriers.
Robert Anders, MD, PhD, Robert L. Ferris, MD, PhD, and Lauren L. Ritterhouse, MD, PhD, prepared useful Practice Aids pertaining to biomarker testing for this CME/MOC activity titled "The Central Role of Biomarker Testing in Piecing Together the Immuno-Oncology Puzzle: Essential Guidance for Pathologists to Maximize the Potential of Cancer Immunotherapies." For the full presentation, monograph, complete CME/MOC information, and to apply for credit, please visit us at http://bit.ly/2QAudYX. CME/MOC credit will be available until December 26, 2019.
A normal cell can be transformed into a cancerous cell. Discuss the therapeutic strategies that are employed to target the cellular transformation process for cancer prevention and treatment.
The field of nanotechnology was first Discovered by Professor Richard P. Feynman in 1959 (Nobel laureate in physics, 1965) [2]. Nanotechnology is the science of the small; very small and it is used for the management of substance at a small scale. At this size, molecules and atoms work in a different way, and provide a variety of unpredicted and attractive uses .
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
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MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
1. UNDER THE GUIDENCE OF:
MRS. D. SANTHI KRUPA
Asst. Professor
DEPT OF PHARMACOLOGY
VIPW
PRESENTED BY:
MEHRAJ. MOHAMMAD
IV yr. B.PHARMACY
A-SECTION
147N1R0024
3. 1. INTRODUCTION
Cancer is a group of diseases involving
abnormal cell growth with the potential to invade
or spread to other parts of the body.
Screening methods are important to find the
solid tumor causing specific agents.
Presently, the existing major anticancer drugs
are having both beneficial and cytotoxic effects.
So as to overcome the undesired effects, new
molecules are to be produced, using simple
cheap methods.
3
4. 2. HISTORY OF ANTICANCER DRUG SCREENING
Initial screening and drug development
programs were small in scale.
Ehrlich and Warburg, studies were
conducted on the tumor growth.
In the early 1950s the program had
evaluated more than 300 chemicals and
several hundreds of plant extracts.
4
5. 3. DEFINITION OF CANCER
Cancer is a group of diseases characterized by
uncontrolled growth and spread of abnormal cells. If the
spread is not controlled, it can result in death.
. Cancer is caused by both external factors and
internal factors
. Cancer is treated with surgery, radiation,
chemotherapy, hormone therapy, biological therapy,
and targeted therapy etc.
5
6. 4. TYPES OF CANCERS
They are noncancerous
They are removed by
surgery and they don’t
reoccur once they
removed
They can be grown
very large and weigh
pounds sometimes
They are cancerous
and it can invade near
by tissues
They reoccur if they
are removed by surgery
These are more
dangerous than benign
tumors
6
7. 7
ANTICANCER DRUGS GENERAL TARGETS
Directly damage the Pre-DNA molecules
Directly Damage the DNA in the nucleus of the cell
Effect the synthesis or breakdown of the mitotic Spindles
9. 5.1TRYPHAN BLUE DYE EXCLUSION ASSAY
Live cells possess intact cell membranes that excludes the dye, where as
dead cells lost membrane integrity take up the dyes
PROCEDURE
Cells are incubated with different concentrations of test
compounds for 4 days.
Dead cells are stained with dyes colors.
Specimen is centrifuged, collected and
observed on microscopic slides.
9
18. Injection of cell suspension
Agitate the cell suspension to prevent the cells from settling, and
withdraw from the sterile tube into a 1-cc TB syringe with the needle
removed.
Lift the skin of the mouse to separate it from the underlying
muscle and inject, with a 21 G needle, 0.1 ml of the cell suspension
(1 × 107
cells) subcutaneously.
Observation
The mice have to be sacrificed to measure tumor volume.
18
19. 6.4 GENETICALLY ENGINEERED CANCER MODELS (GEM)
GEM models have increased understanding of the molecular
pathways responsible for the initiation and progression of human
cancer, and have highlighted the importance of specific oncogenes
and tumor suppressor genes (TSG) in particular types of cancer.
GEM models possess well-validated molecular/genetic
characteristics which ultimately facilitate the rational design of
small molecule therapeutics.
19
20. 7. CONCLUSION
Anticancer drug screening is necessary to prioritize compounds for
further development. In the era of target oriented molecular cancer
therapeutics, screening procedures are tailored towards the specific area.
They however require, careful design and validation. So, knowledge on
this screening methods can lay a path for screening of the novel anticancer
drugs with more selectivity and specificity.
20
21. 8. REFERENCES
• Kumar V, Abbas AK, Aster JC. Robbins Basic Pathology. 9th ed.
Philadelphia, PA: Saunders. 2013. , Neoplasm; pp. 161–214.
• Talmadge JE, Singh RK, Fidler IJ, Raz A. Murine models to evaluate
novel and conventional therapeutic strategies for cancer. Am J Pathol.,
2007;170:793–804.
• Farber S. Some observations on the effect of folic acid antagonists on acute
leukemia and other forms of incurable cancer. Blood.,1949;4:160–7.
• Elion GB, Singer S, Hitchings GH. Antagonists of nucleic acid derivatives.
VIII. Synergism in combinations of biochemically related
antimetabolites. J Biol Chem., 1954;208:477–88.
21