Lyophilization or freeze drying is a process in which water is removed from a product after it is frozen and placed under a vacuum, allowing the ice to change directly from solid to vapor without passing through a liquid phase. The process consists of three separate, unique, and interdependent processes; freezing, primary drying (sublimation), and secondary drying (desorption).
The advantages of lyophilization include:
Ease of processing a liquid, which simplifies aseptic handling
Enhanced stability of a dry powder
Removal of water without excessive heating of the product
Enhanced product stability in a dry state
Rapid and easy dissolution of reconstituted product
Disadvantages of lyophilization include:
Increased handling and processing time
Need for sterile diluent upon reconstitution
Cost and complexity of equipment
The lyophilization process generally includes the following steps:
Dissolving the drug and excipients in a suitable solvent, generally water for injection (WFI).
Sterilizing the bulk solution by passing it through a 0.22 micron bacteria-retentive filter.
Filling into individual sterile containers and partially stoppering the containers under aseptic conditions.
Transporting the partially stoppered containers to the lyophilizer and loading into the chamber under aseptic conditions.
Freezing the solution by placing the partially stoppered containers on cooled shelves in a freeze-drying chamber or pre-freezing in another chamber.
Applying a vacuum to the chamber and heating the shelves in order to evaporate the water from the frozen state.
Complete stoppering of the vials usually by hydraulic or screw rod stoppering mechanisms installed in the lyophilizers.
There are many new parenteral products, including anti-infectives, biotechnology derived products, and in-vitro diagnostics which are manufactured as lyophilized products. Additionally, inspections have disclosed potency, sterility and stability problems associated with the manufacture and control of lyophilized products. In order to provide guidance and information to investigators, some industry procedures and deficiencies associated with lyophilized products are identified in this Inspection Guide.
It is recognized that there is complex technology associated with the manufacture and control of a lyophilized pharmaceutical dosage form. Some of the important aspects of these operations include: the formulation of solutions; filling of vials and validation of the filling operation; sterilization and engineering aspects of the lyophilizer; scale-up and validation of the lyophilization cycle; and testing of the end product. This discussion will address some of the problems associated with the manufacture and control of a lyophilized dosage form.
The process of freeze drying with greater emphasis on the uses in the fisheries food processing sector. The presentation shows the process involved and the different steps involved and the effect of the process on the food material.
Definition of drying
Importance of drying
Difference between drying and evaporation
Equipments
References
Definition
A stabilizing process in which a substance is first frozen and then the quantity of the solvent is reduced, first by sublimation (primary drying stage) and then desorption (secondary drying stage) to values that will no longer support biological activity or chemical reactions.
History
Freeze drying was first actively developed during WORLD WAR II transport of serum.
The main aim was to store the products without refrigeration and to remove moisture from thermolabile compounds.
Atlas in 1961 built 6 production freeze drying cabinet for Nestle group in Germany, Holland.
Principle
Lyophilization is carried out using a simple principle of physics sublimation. Sublimation is the transition of a substance from the solid to the vapour state, without first passing through an intermediate liquid phase.
Lyophilization is performed at temperature and pressure conditions below the triple point, to enable sublimation of ice.
The entire process is performed at low temperature and pressure by applying vacuum, hence is suited for drying of thermolabile compounds.
The concentration gradient of water vapour between the drying front and condenser is the driving force for removal of water during lyophilization.
Freeze drying pharmaceuticals uses a process called lyophilization to lower the temperature of the product to below freezing, and then a high-pressure vacuum is applied to extract the water in the form of vapour. The vapour collects on a condenser, turns back to ice and is removed.
Definition and Objectives of Lyophilization, Advantages & Disadvantages, Basic Principles of Lyophilization, Steps of Lyophilization,
Main Components of Lyophilizer,
Qualification of Lyophilizer,
Development of Lyophilization cycle, Defects of Lyophilizer.
I'm delighted to share the PDFs of lab courses in Microbial Physiology and Microbial Genetics. These comprehensive resources cover essential topics in understanding the intricate workings of microbes at a physiological and genetic level. these PDFs provide a detailed roadmap for our laboratory explorations.
Microbial physiology is the study of the structure, growth factors, metabolic activities, nutritional requirements, and genetic composition of microorganismsThe microbial cells are extremely complex and in addition to oxygen and hydrogen they contain four other major elements such as carbon, nitrogen, phosphorus and sulphur. here are the notes on microbial physiology..Photosynthetic pigments are the molecules responsible for absorbing electromagnetic radiation, transferring the energy of the absorbed photons to the reaction center, and for photochemical conversion in the photosynthetic systems of organisms capable of photosynthesis.
The process of freeze drying with greater emphasis on the uses in the fisheries food processing sector. The presentation shows the process involved and the different steps involved and the effect of the process on the food material.
Definition of drying
Importance of drying
Difference between drying and evaporation
Equipments
References
Definition
A stabilizing process in which a substance is first frozen and then the quantity of the solvent is reduced, first by sublimation (primary drying stage) and then desorption (secondary drying stage) to values that will no longer support biological activity or chemical reactions.
History
Freeze drying was first actively developed during WORLD WAR II transport of serum.
The main aim was to store the products without refrigeration and to remove moisture from thermolabile compounds.
Atlas in 1961 built 6 production freeze drying cabinet for Nestle group in Germany, Holland.
Principle
Lyophilization is carried out using a simple principle of physics sublimation. Sublimation is the transition of a substance from the solid to the vapour state, without first passing through an intermediate liquid phase.
Lyophilization is performed at temperature and pressure conditions below the triple point, to enable sublimation of ice.
The entire process is performed at low temperature and pressure by applying vacuum, hence is suited for drying of thermolabile compounds.
The concentration gradient of water vapour between the drying front and condenser is the driving force for removal of water during lyophilization.
Freeze drying pharmaceuticals uses a process called lyophilization to lower the temperature of the product to below freezing, and then a high-pressure vacuum is applied to extract the water in the form of vapour. The vapour collects on a condenser, turns back to ice and is removed.
Definition and Objectives of Lyophilization, Advantages & Disadvantages, Basic Principles of Lyophilization, Steps of Lyophilization,
Main Components of Lyophilizer,
Qualification of Lyophilizer,
Development of Lyophilization cycle, Defects of Lyophilizer.
I'm delighted to share the PDFs of lab courses in Microbial Physiology and Microbial Genetics. These comprehensive resources cover essential topics in understanding the intricate workings of microbes at a physiological and genetic level. these PDFs provide a detailed roadmap for our laboratory explorations.
Microbial physiology is the study of the structure, growth factors, metabolic activities, nutritional requirements, and genetic composition of microorganismsThe microbial cells are extremely complex and in addition to oxygen and hydrogen they contain four other major elements such as carbon, nitrogen, phosphorus and sulphur. here are the notes on microbial physiology..Photosynthetic pigments are the molecules responsible for absorbing electromagnetic radiation, transferring the energy of the absorbed photons to the reaction center, and for photochemical conversion in the photosynthetic systems of organisms capable of photosynthesis.
Probability is the branch of mathematics concerning events and numerical descriptions of how likely they are to occur. The probability of an event is a number between 0 and 1; the larger the probability, the more likely an event is to occur.[note 1][1][2] The higher the probability of an event, the more likely it is that the event will occur. A simple example is the tossing of a fair (unbiased) coin. Since the coin is fair, the two outcomes ('heads' and 'tails') are both equally probable; the probability of 'heads' equals the probability of 'tails'; and since no other outcomes are possible, the probability of either 'heads' or 'tails' is 1/2 (which could also be written as 0.5 or 50%).
These concepts have been given an axiomatic mathematical formalization in probability theory, which is used widely in areas of study such as statistics, mathematics, science, finance, gambling, artificial intelligence, machine learning, computer science, game theory, and philosophy to, for example, draw inferences about the expected frequency of events. Probability theory is also used to describe the underlying mechanics and regularities of complex systems.
Plasmids are extrachromosomal DNA molecules. They are small, circular and have the ability to replicate autonomously. Replication of plasmid is not under the control of chromosomal DNA. They are mostly found in bacteria. Some of the eukaryotes like yeast and plants also contain plasmids.
Download Complete Chapter Notes of Biotechnology: Principles and Processes
Their ability to replicate independently makes plasmid a cloning vector in the recombinant DNA technology for transferring and manipulating genes.
Many antibiotic-resistant genes in bacteria are present in plasmids.
The size of plasmid varies from a few base pairs to thousands of bp.
Plasmids also get transferred from one bacterial cell to another by the process of conjugation.
Plasmids carrying a specific gene are introduced into bacterial cells, which multiply rapidly and the required DNA fragment is produced in larger quantities.
Plasmids are used to prepare recombinant DNA with the desired gene to transfer genes from one organism to another. This is known as genetic engineering.
Joshua Lederberg coined the term plasmid.
mutagen is a physical or chemical agent that permanently changes genetic material, usually DNA, in an organism and thus increases the frequency of mutations above the natural background level. As many mutations can cause cancer in animals, such mutagens can therefore be carcinogens, although not all necessarily are. All mutagens have characteristic mutational signatures with some chemicals becoming mutagenic through cellular processes.
The process of DNA becoming modified is called mutagenesis. Not all mutations are caused by mutagens: so-called "spontaneous mutations" occur due to spontaneous hydrolysis, errors in DNA replication, repair and recombination.
Discovery
The first mutagens to be identified were carcinogens, substances that were shown to be linked to cancer. Tumors were described more than 2,000 years before the discovery of chromosomes and DNA; in 500 B.C., the Greek physician Hippocrates named tumors resembling a crab karkinos (from which the word "cancer" is derived via Latin), meaning crab.[1] In 1567, Swiss physician Paracelsus suggested that an unidentified substance in mined ore (identified as radon gas in modern times) caused a wasting disease in miners,[2] and in England, in 1761, John Hill made the first direct link of cancer to chemical substances by noting that excessive use of snuff may cause nasal cancer.[3] In 1775, Sir Percivall Pott wrote a paper on the high incidence of scrotal cancer in chimney sweeps, and suggested chimney soot as the cause of scrotal cancer.[4] In 1915, Yamagawa and Ichikawa showed that repeated application of coal tar to rabbit's ears produced malignant cancer.[5] Subsequently, in the 1930s the carcinogen component in coal tar was identified as a polyaromatic hydrocarbon (PAH), benzo[a]pyrene.
Polyaromatic hydrocarbons are also present in soot, which was suggested to be a causative agent of cancer over 150 years earlier.
A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that "does not occur naturally by mating and/or natural recombination".[1] A wide variety of organisms have been genetically modified (GM), including animals, plants, and microorganisms.
Genetic modification can include the introduction of new genes or enhancing, altering, or knocking out endogenous genes. In some genetic modifications, genes are transferred within the same species, across species (creating transgenic organisms), and even across kingdoms.
Creating a genetically modified organism is a multi-step process. Genetic engineers must isolate the gene they wish to insert into the host organism and combine it with other genetic elements, including a promoter and terminator region and often a selectable marker. A number of techniques are available for inserting the isolated gene into the host genome. Recent advancements using genome editing techniques, notably CRISPR, have made the production of GMOs much simpler. Herbert Boyer and Stanley Cohen made the first genetically modified organism in 1973, a bacterium resistant to the antibiotic kanamycin. The first genetically modified animal, a mouse, was created in 1974 by Rudolf Jaenisch, and the first plant was produced in 1983. In 1994, the Flavr Savr tomato was released, the first commercialized genetically modified food. The first genetically modified animal to be commercialized was the GloFish (2003) and the first genetically modified animal to be approved for food use was the AquAdvantage salmon in 2015.
Nitrogen-fixing bacteria, microorganisms capable of transforming atmospheric nitrogen into fixed nitrogen (inorganic compounds usable by plants). More than 90 percent of all nitrogen fixation is effected by these organisms, which thus play an important role in the nitrogen cycle.
Two kinds of nitrogen-fixing bacteria are recognized. The first kind, the free-living (nonsymbiotic) bacteria, includes the cyanobacteria (or blue-green algae) Anabaena and Nostoc and genera such as Azotobacter, Beijerinckia, and Clostridium. The second kind comprises the mutualistic (symbiotic) bacteria; examples include Rhizobium, associated with leguminous plants (e.g., various members of the pea family); Frankia, associated with certain dicotyledonous species (actinorhizal plants); and certain Azospirillum species, associated with cereal grasses.
The symbiotic nitrogen-fixing bacteria invade the root hairs of host plants, where they multiply and stimulate formation of root nodules, enlargements of plant cells and bacteria in intimate association. Within the nodules the bacteria convert free nitrogen to ammonia, which the host plant utilizes for its development. To ensure sufficient nodule formation and optimum growth of legumes (e.g., alfalfa, beans, clovers, peas, soybeans), seeds are usually inoculated with commercial cultures of appropriate Rhizobium species, especially in soils poor or lacking in the required bacterium.
Ecosystems can be as large as a desert or as small as a puddle.
They contain biotic
or living parts, as well as abiotic factors
, or nonliving parts. Biotic factors include plants, animals, and other organisms.
Nonliving materials include water, rocks, soil, and sand.
Ecosystems are a chain of interactions between organisms and their environment.
Each organism has a "job" to do in the ecosystem. For example, plants have chloroplasts that enable them to harvest light energy. Then, they take carbon dioxide and water from their environment to convert them into sugar.
Many ecosystems become degraded through human impacts, such as soil loss, air and water pollution, habitat fragmentation
, water diversion, fire suppression, and introduced species
and invasive species
.
An ecosystem includes all the living things (plants, animals and organisms) in a given area, interacting with each other, and with their non-living environments (weather, earth, sun, soil, climate, atmosphere). In an ecosystem, each organism has its own niche or role to play.
L-Lysine is an essential amino acid that's produced by fermentation. The fermentation process uses selected strains of microorganisms that grow in a solution of glucose or molasses, ammonium compounds, inorganic salts, and other substances.
Industrial lysine fermentation is usually performed using large-scale tank fermenters. In production plants, lysine accumulates to a final titer of 170 g/L after 45 hours.
Lysine and other amino acids are commonly produced by fermentation using strains of heterotrophic bacteria, such as Escherichia coli and Corynebacterium glutamicum. C. glutamicum has been engineered to produce lysine with a yield of 0.31 g lysine/g sugar.
Lysine is involved in the production of hormones and energy. It's also important for calcium and immune function.
Amino acids are small molecules that are the building blocks of proteins. Proteins serve as structural support inside the cell and they perform many vital chemical reactions. Each protein is a molecule made up of different combinations of 20 types of smaller, simpler amino acids.The pathways of amino acid synthesis comprise a significant fraction of a bacterium's metabolic activity during its growth in a minimal medium. Two amino acids, glutamine and glutamate, are the immediate products of ammonia assimilation and essential nitrogen donors for the synthesis of other intermediates.
Nutrition is substances used in biosynthesis and energy production and therefore are required for all living things.
Bacteria, like all living cells, require energy and nutrients to build proteins and structural membranes and drive biochemical processes.
Bacteria require sources of carbon, nitrogen, phosphorous, iron and a large number of other molecules.
Carbon, nitrogen, and water are used in the highest quantities.
The nutritional requirements for bacteria can be grouped according to the carbon source and the energy source.
Some types of bacteria must consume pre-formed organic molecules to obtain energy, while other bacteria can generate their own energy from inorganic sources.
Ascomycota is a phylum of the kingdom Fungi that, together with the Basidiomycota, forms the subkingdom Dikarya. Its members are commonly known as the sac fungi or ascomycetes. It is the largest phylum of Fungi, with over 64,000 species.The defining feature of this fungal group is the "ascus" (from Ancient Greek ἀσκός (askós) 'sac, wineskin'), a microscopic sexual structure in which nonmotile spores, called ascospores, are formed. However, some species of the Ascomycota are asexual, meaning that they do not have a sexual cycle and thus do not form asci or ascospores. Familiar examples of sac fungi include morels, truffles, brewers' and bakers' yeast, dead man's fingers, and cup fungi. The fungal symbionts in the majority of lichens (loosely termed "ascolichens") such as Cladonia belong to the Ascomycota.
Ascomycota is a monophyletic group (it contains all descendants of one common ancestor). Previously placed in the Deuteromycota along with asexual species from other fungal taxa, asexual (or anamorphic) ascomycetes are now identified and classified based on morphological or physiological similarities to ascus-bearing taxa, and by phylogenetic analyses of DNA sequences.
A fungus or funguses is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and molds, as well as the more familiar mushrooms. These organisms are classified as a kingdom, separately from the other eukaryotic kingdoms, which, by one traditional classification, includes Plantae, Animalia, Protozoa, and Chromista.
A characteristic that places fungi in a different kingdom from plants, bacteria, and some protists is chitin in their cell walls. Fungi, like animals, are heterotrophs; they acquire their food by absorbing dissolved molecules, typically by secreting digestive enzymes into their environment. Fungi do not photosynthesize. Growth is their means of mobility, except for spores (a few of which are flagellated), which may travel through the air or water. Fungi are the principal decomposers in ecological systems. These and other differences place fungi in a single group of related organisms, named the Eumycota (true fungi or Eumycetes), that share a common ancestor (i.e. they form a monophyletic group), an interpretation that is also strongly supported by molecular phylogenetics. This fungal group is distinct from the structurally similar myxomycetes (slime molds) and oomycetes (water molds). The discipline of biology devoted to the study of fungi is known as mycology (from the Greek μύκης mykes, mushroom). In the past mycology was regarded as a branch of botany, although it is now known that fungi are genetically more closely related to animals than to plants.
Abundant worldwide, most fungi are inconspicuous because of the small size of their structures, and their cryptic lifestyles in soil or on dead matter. Fungi include symbionts of plants, animals, or other fungi and also parasites. They may become noticeable when fruiting, either as mushrooms or as molds. Fungi perform an essential role in the decomposition of organic matter and have fundamental roles in nutrient cycling and exchange in the environment. They have long been used as a direct source of human food, in the form of mushrooms and truffles; as a leavening agent for bread; and in the fermentation of various food products, such as wine, beer, and soy sauce. Since the 1940s, fungi have been used for the production of antibiotics, and, more recently, various enzymes produced by fungi are used industrially and in detergents. Fungi are also used as biological pesticides to control weeds, plant diseases, and insect pests. Many species produce bioactive compounds called mycotoxins, such as alkaloids and polyketides, that are toxic to animals, including humans. The fruiting structures of a few species contain psychotropic compounds and are consumed recreationally or in traditional spiritual ceremonies. Fungi can break down manufactured materials and buildings, and become significant pathogens of humans and other animals. Losses of crops due to fungal diseases (e.g., rice blast disease) or food spoilage can have a large impact on
Basidiomycetes
Also known as club fungi.
The reproductive structure is basidium.
Spores that are produced are called basidiospores.
The vegetative structure is made up of primary or secondary mycelium.
Vegetative reproduction is done by fragmentation.
Sexual reproduction is done by the two vegetative or somatic cells creating a basidium.
Basidiospores are produced in the basidium by the development of fruiting bodies called basidiocarps.
Examples – Agaricus (found in mushrooms), Puccinia.
Basidiomycetes include these groups: mushrooms, puffballs, jelly fungi, boletes, chanterelles, earth stars, smuts, rusts, mirror yeasts, and Cryptococcus, the human pathogenic yeast.
Lipid metabolism is the synthesis and degradation of lipids in cells, involving the breakdown and storage of fats for energy and the synthesis of structural and functional lipids, such as those involved in the construction of cell membranes. In animals, these fats are obtained from food and are synthesized by the liver. Lipogenesis is the process of synthesizing these fats. The majority of lipids found in the human body from ingesting food are triglycerides and cholesterol.[4] Other types of lipids found in the body are fatty acids and membrane lipids. Lipid metabolism is often considered as the digestion and absorption process of dietary fat; however, there are two sources of fats that organisms can use to obtain energy: from consumed dietary fats and from stored fat.[5] Vertebrates (including humans) use both sources of fat to produce energy for organs such as the heart to function. Since lipids are hydrophobic molecules, they need to be solubilized before their metabolism can begin. Lipid metabolism often begins with hydrolysis, which occurs with the help of various enzymes in the digestive system.Lipid metabolism also occurs in plants, though the processes differ in some ways when compared to animals.[8] The second step after the hydrolysis is the absorption of the fatty acids into the epithelial cells of the intestinal wall.[6] In the epithelial cells, fatty acids are packaged and transported to the rest of the body.[9]
Metabolic processes include lipid digestion, lipid absorption, lipid transportation, lipid storage, lipid catabolism, and lipid biosynthesis. Lipid catabolism is accomplished by a process known as beta oxidation which takes place in the mitochondria and peroxisome cell organelles.
A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of DNA. These enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex. During this process, DNA polymerase "reads" the existing DNA strands to create two new strands that match the existing ones.[1][2][3][4][5][6] These enzymes catalyze the chemical reaction
deoxynucleoside triphosphate + DNAn ⇌ pyrophosphate + DNAn+1.
DNA polymerase adds nucleotides to the three prime (3')-end of a DNA strand, one nucleotide at a time. Every time a cell divides, DNA polymerases are required to duplicate the cell's DNA, so that a copy of the original DNA molecule can be passed to each daughter cell. In this way, genetic information is passed down from generation to generation.
Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form, in the process breaking the hydrogen bonds between the nucleotide bases. This opens up or "unzips" the double-stranded DNA to give two single strands of DNA that can be used as templates for replication in the above reaction.
Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. This spectroscopy is based on the measurement of absorption of electromagnetic radiations in the radio frequency region from roughly 4 to 900 MHz. Absorption of radio waves in the presence of magnetic field is accompanied by a special type of nuclear transition, and for this reason, such type of spectroscopy is known as Nuclear Magnetic Resonance Spectroscopy.[1] The sample is placed in a magnetic field and the NMR signal is produced by excitation of the nuclei sample with radio waves into nuclear magnetic resonance, which is detected with sensitive radio receivers. The intramolecular magnetic field around an atom in a molecule changes the resonance frequency, thus giving access to details of the electronic structure of a molecule and its individual functional groups. As the fields are unique or highly characteristic to individual compounds, in modern organic chemistry practice, NMR spectroscopy is the definitive method to identify monomolecular organic compounds.
The principle of NMR usually involves three sequential steps:
The alignment (polarization) of the magnetic nuclear spins in an applied, constant magnetic field B0.
The perturbation of this alignment of the nuclear spins by a weak oscillating magnetic field, usually referred to as a radio-frequency (RF) pulse.
Detection and analysis of the electromagnetic waves emitted by the nuclei of the sample as a result of this perturbation.
Similarly, biochemists use NMR to identify proteins and other complex molecules. Besides identification, NMR spectroscopy provides detailed information about the structure, dynamics, reaction state, and chemical environment of molecules. The most common types of NMR are proton and carbon-13 NMR spectroscopy, but it is applicable to any kind of sample that contains nuclei possessing spin.
NMR spectra are unique, well-resolved, analytically tractable and often highly predictable for small molecules. Different functional groups are obviously distinguishable, and identical functional groups with differing neighboring substituents still give distinguishable signals. NMR has largely replaced traditional wet chemistry tests such as color reagents or typical chromatography for identification. A disadvantage is that a relatively large amount, 2–50 mg, of a purified substance is required, although it may be recovered through a workup. Preferably, the sample should be dissolved in a solvent, because NMR analysis of solids requires a dedicated magic angle spinning machine and may not give equally well-resolved spectra. The timescale of NMR is relatively long, and thus it is not suitable for observing fast phenomena, producing only an averaged spectrum. Although large amounts of impurities do show on an NMR spectrum, better methods exist for detecting impurities.
Electrophoresis is a scientific laboratory technique that is used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is passed through the molecules to move them so that they can be separated via a gel. The pores present in the gel work like a sieve, allowing smaller molecules to pass through more quickly and easily than the larger molecules. According to the way conditions are adjusted during electrophoresis, the molecules can be separated in the desired size range.
What is electrophoresis and what are its uses?
Electrophoresis is a very broadly used technique that, fundamentally, applies electric current to biological molecules – they’re usually DNA, but they can be protein or RNA, too – and separates these fragments into pieces that are larger or smaller in size.
The phenomenon of electrophoresis was first observed by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss in 1807 at Moscow University. A constant application of electric field caused the particles of clay dispersed in water to migrate, showing an electrokinetic phenomenon.
Electrophoresis can be defined as an electrokinetic process that separates charged particles in a fluid using an electrical field of charge. Electrophoresis of cations or positively charged ions is sometimes referred to as cataphoresis (or cataphoretic electrophoresis). In contrast, sometimes, the electrophoresis of anions or negatively charged ions is referred to as anaphoresis (or anaphoric electrophoresis).
It’s used in a variety of applications. Though it is most often used in life sciences to separate protein molecules or DNA, it can be achieved through several different techniques and methods depending upon the type and size of the molecules.
The methods differ in some ways, but all we need is a source for the electrical charge, a support medium and a buffer solution. Electrophoresis is also used in laboratories for the separation of molecules based on their size, density and purity.
The method used to separate macromolecules such as DNA, RNA, or protein molecules is known as gel electrophoresis.
It is used in forensics for –
Nucleic acid molecule sizing
DNA fragmentation for southern blotting
RNA fragmentation for northern blotting
Protein fragmentation for western blotting
Separation of PCR products analysis
Detection and analysis of variations or mutations in the sequence
Its clinical applications involve –
Serum protein electrophoresis
Lipoprotein analysis
Diagnosis of haemoglobinopathies and hemoglobin A1c.
The fundamental principle of electrophoresis is the existence of charge separation between the surface of a particle and the fluid immediately surrounding it. An applied electric field acts on the resulting charge density, causing the particle to migrate and the fluid around the particle to flow.
It is the process of separation or purification of protein molecules, DNA, or RNA that differ in charge, size.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
"Protectable subject matters, Protection in biotechnology, Protection of othe...
Lyophilizationn.pdf
1. INTRODUCTION :-
• Lyophilization is also known as Freeze Drying or Cryodesiccation is a dehydration
process typically used to preserve a perishable material or made the material more
convenient for transport.
• It is a special form of drying that removes all moistures and tends to have less effect on
a food’s taste than normal dehydration process.
• In this process , food is frozen and placed in a strong vacuum . The water in the food
sublimates , it turns straight from ice into vapour.
• Process was first applied to the food products after the second world war.
• Coffee was first freeze dried food product to be produced.
DEFINITION :-
A stabilizing process in which a substance is first frozen and then the quantity of the solvent is
reduced , first by sublimation (primary drying stage) and then desorption (secondary drying
stage) to values that will no longer support biological activity or chemical reactions.
PRINCIPLE :-
• Lyophilization is carried out using a simple principle of physics sublimation. Sublimation
is the transition of a substance from the solid to the vapour state , without first passing
through an intermediate liquid phase.
• Lyophilization is performed at temperature and pressure conditions below the triple
point , to enable sublimation of ice.
• Triple point of water is a point where , water exists in all the three phases, the solid , the
liquid and the gas. This point is achieved at the temperature of 0.01°C and the pressure
of 0.00603 atm.
• The entire process is performed at low temperature and pressure by applying vacuum ,
hence is suited for drying of thermolabile compounds.
• So , when the process is performed below these conditions the solid ice is directly
converted into the gaseous form without converting into the liquid.
• So , in this technique thr liquid is frozen to solid and then is converted to gas , to remove
all the moisture or solvent present in it.
2. TYPES :-
The types of lyophilizer are classified as –
1. On the basis of their size.
2. On the basis of their capacity.
1. On the basis of size :- Depending upon the size they can be classified as –
A Typical Manifold Dryer
B Shelf Freeze Dryer
C Combination Lab Freeze Dryer
2. On the basis of capacity :- Depending upon the capacity they can be classified as –
A Bench Top Freeze Dryer
B Pivot Freeze Dryer
C Production Freeze Dryer
COMPONENTS OF LYOPHILIZER :-
The main components of lyophilizer are –
• Chamber
• Shelves
• Refrigerator System
• Condenser
• Vacuum Pump
3. 1. Chamber :- A chamber is tight insulated box pf stainless steel consisting of shelves.
2. Shelves :- Shelves on which products are kept and acts as a heat exchanger.
3. Refrigerator System :- A refrigerator system , which has a compressor which cools the
condenser.
4. Condenser :- Condenser is a form of coils or plates for trapping solvent vapours.
5. Vacuum Pump :- A vacuum pump is there for lowering the temperature.
PROCESS OF LYOPHILIZATION :-
The process of lyophilization involves following steps –
1. Pre treatment
2. Freezing Stage
3. Primary Drying Stage
4. Secondary Drying Stage
5. Packing
4. 1. Pre Treatment :-
• This stage include all the methods of treating the product prior to freezing
• Such as – concentrating the product to make it stable such that the minimum amount of
the solvent is present from the beginning only.
• Formulation revision i.e addition of cryoprotectants.
• Decreasing the high vapour pressure solvent.
• Increasing the surface area.
2. Freezing Stage or Freeze Drying Stage :
• In this stage the product is frozen to ice.
• The product is frozen below it’s euthentic temperature.
• Low temperature and pressure is maintained.
• Decreasing the shelf temperature to -50°C.
• In the laboratory , it is done by placing the product in the freeze dryer flask , which is
rotated in the shell freezer bath , which is cooled by dry ice , methanol or mechanical
refrigeration etc.
3. Primary Drying [ Sublimation ]:
▪ In the stage the temperature and pressure are kept below the triple point of water .
▪ That is the temperature.0.0098°C and pressure 4.58 mmHg
▪ Heat is introduced from the shelf to the product under the granted control by electrical
resistance cells or circulating silicon.
▪ Rate of sublimation of ice depends upon difference in V.P of product ad V.P of
condenser
▪ 95-98% of Water is removed , and process takes 20-24 hours .
4. Secondary Drying [ Desorption ]:
▪ It is also known as Isothermal Desorption
▪ Which removes the remaining solvent form the product .
▪ It removes the bound water .
▪ It is continued at same pressure but the temperature is increased around +10-40°C
▪ It takes about 10-20hrs.
5. 5. Packing :
▪ After drying the vaccum is replaced by filtered air or nitrogen to establish atmospheric
pressure .
▪ Ampoules are sealed by either tip sealing or clousers and aluminium caps .
▪ Vials and bottles are sealed with rubber clousers and alluminium caps.
APPLICATIONS OF LYOPHILIZATION :-
Following are the applications of lyophilization –
1. Pharmaceutical Industry and Biotechnology :-
• Pharmaceutical companies often use freeze drying to increase the shelf life of products ,
such as vaccines and other injectable.
• By removing the water from the material and sealing the material in a vial , the material
can be easily stored , shipped and later reconstituted to its original form for injection.
2. Food Industry :-
• Freeze – drying is used to preserve food and make it very light weight.
• The process has been popularized in the forms of freeze dried icecream ; an example of
astronaut food.
• Instant coffee powder is prepared.
• To produce essences or flavouring agents.
• Culinary herbs are preserved.
• Freeze dried fruits are produced.
3. Technological Industries :-
• In chemical synthesis , products are often lyophilized to make them more stable , or
easier to dissolve in water for subsequent use.
• It is also capable of concentrating substances with low molecular weights that are two
small to be removed by a filtration membrane.
4. Other uses :-
• Freeze-drying is used for flora and fauna preservation.
• It is also used for recovery of water- damaged books and documents.
6. ADVANTAGES OF LYOPHILIZATION :-
Following are the advantages of lyophilization –
• Minimum damage to heat labile material.
• Removal of water at low temperature.
• Compatible with aseptic operations.
• Sterility can be maintained.
• Reconstitution is easy.
• Increasing shelf life of the product.
• Doesnot shrink the samples.
• Reduces weight and volume of samples ; ideal for shipping .
• Samples can be stored at room temperature indefinitely.
DISADVANTAGES OF LYOPHILIZATION :-
Following are the disadvantages of lyophilization –
• High capital cost of equipment.
• High energy cost.
• Time consuming process ( Typically 4-10 hrs per drying cycle ).
• Possible damage to products due to change in pH and tonicity.
• Many biological molecules are damaged by the stress associated with freezing.
• The open porous structure of the food may allow oxygen to enter and cause oxidative
degradation of lipids (packing in inert gas necessary).
