This document discusses liquid chromatography-mass spectrometry (LC-MS). It begins with an introduction to LC-MS, explaining that it combines liquid chromatography separation with mass spectrometry detection. It then covers the history, principles, instrumentation, advantages, and applications of LC and MS. Key applications discussed include pharmacokinetics, proteomics, metabolomics, and drug development.
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
detail notes on connective tissue..
Connective tissue (CT) is one of the four basic types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. It develops from the mesoderm. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. In the central nervous system, the three outer membranes (the meninges) that envelop the brain and spinal cord are composed of connective tissue.
All connective tissue consists of three main components: fibers (elastic and collagenous fibers), ground substance and cells. Not all authorities include blood or lymph as connective tissue because they lack the fiber component. All are immersed in the body water.
SEPARATION, BIOANALYTICAL TECHNIQUES, EXTRACTION, LC MS, LIQUID CHROMATOGRAPHY AND MASS SPECTROSCOPY, MBAT, M.PHARM, PHARMACEUTICAL ANALYSIS, 1 ST YEAR
The aim of the coupling is to obtain an information-rich detection for both identification and quantification compared to that with a single analytical technique.
METABOLOMICS is the systematic study of the small molecular metabolites in a cell, tissue, biofluid, or cell culture media that are the tangible result of cellular processes or responses to an environmental stress.
• It is the combination of liquid chromatography and the mass spectrometry.
• Liquid chromatography-mass spectrometry (LC-MS) is an analytical chemistry
technique that combines the physical separation capabilities of liquid
chromatography with the mass analysis capabilities of mass spectrometry.
• The combination of these two powerful techniques gives the chemical analyst the
ability to analyze virtually any molecular species; including, thermally labile, non
volatile, and high molecular weight species.
Chromatography is a technique for separating various inorganic and organic compounds. It is one of the separation techniques used as differential migration. It is more advantageous over conventional separating methods such as crystallization, solvent extraction and distillation. The purpose of presentation is to present various chromatographic techniques included a few advanced forms such as FC, HPLC,UPLC and UPCC (Super Critical chromatography).These are rapid forms of chromatographic techniques based on air pressure driven, optimized for rapid and precise separation of an organic compound.
Hyphenated technique is a combination or coupling of two analytical techniques with the help of proper interface.
In this presentation Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS has been discussed
The combined technique between MS and HPLC is commonly known as LC-MS. Combining the two analytical methods reduces experimental error and improves accuracy. The application of LC-MS is very useful in situations that involve a huge number of compounds, such as environmental effluents.
Metabolomics is often described as the study of “the complete set of low molecular weight intermediates, which are context dependent, varying according to the physiology, developmental or pathological state of the cell, tissue, organ or organism”. In fact, metabolomics is a new term for an old science in which classical biochemical concepts are investigated. New and unique to the current research that is being conducted is the combination with genomics information and full system biology. In this refocus we will discuss the challenges in today's metabolomics research and how to address them
Antipyretic, Antimicrobial and Anti-inflammatory activity of Kabasura kudinee...GOPALASATHEESKUMAR K
This study is designed to evaluate the anti-inflammaatory, antipyretic aqueous extract of Kabasurakudineerchoornam (AEKKC). The estimated by Folin-Ciocalteu and Aluminium chloride colorimetric potential was determined by DPPH and ABTS radical scavenging any signs of toxicity upto 2000 mg/kg in rats. The anti-inflammatory evaluated by carrageenan-induced paw edema and histamine induced of AEKKC at 200 and 400 mg/kg showed significant reduction in both carrageenan and histamine induced inflammation. The and 400 mg/kg) was studied by brewer’s yeast induced pyrexia mg/kg) was used as standard. The extract showed significant evoked elevated body temperature. AEKKC also exhibited antibacterial inhibition via disc diffusion method. The results of the study prove inflammatory, antipyretic and antimicrobial activity.
The aim of the present research was formulation and evaluation of anti-inflammatory drug dexibuprofen loaded chitosan-based polymeric nanoparticles (NPs) for the controlled release of dexibuprofen using different concentrations of chitosan and surfactant. Materials and Methods: Dexibuprofen, a nonsteroidal anti-inflammatory drug was encapsulated with the polymer by emulsion-droplet coalescence method (DNP1-DNP5). The NPs were characterized by drug content, particle size, zeta potential, encapsulation efficiency, and in vitro drug release. Result: DNP3 was selected as best formulation due to its ideal particle size (437.6 nm), high entrapment efficiency (88.54%), and desirable drug release (99.81 ± 0.92% at the end of 24 h). Conclusion: The present study can be concluded that the newly formulated controlled release nanoparticulate drug delivery system of dexibuprofen may be ideal and effective in the management of pain due to arthritis by allowing the drug to release continuously for 24 h.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
3. INTRODUCTION
Liquid chromatography–mass spectrometry (LC-MS) is an
analytical chemistry technique that combines the physical
separation capabilities of liquid chromatography (or HPLC) with
the mass analysis capabilities of mass spectrometry (MS).
liquid chromatography separates mixtures with multiple
components, mass spectrometry provides structural identity of
the individual components with high molecular specificity and
detection sensitivity.
This bicycle technique can be used to analyze biochemical,
organic, and inorganic compounds commonly found in complex
samples of environmental and biological origin.
Therefore, LC-MS may be applied in a wide range of sectors
including biotechnology, environment monitoring, food
processing, and pharmaceutical, agrochemical, and cosmetic
industries.
9/3/2017 KMCH COLLEGE OF PHARMACY 3
4. HISTORY OF LC-MS
The coupling of chromatography with MS is a well
developed chemical analysis strategy dating back from the
1950s.
the development of GC-MS systems was faster than LC-MS
and such systems were first commercialized in the 1970s.
In general, off-line coupling involved fraction collection,
evaporation of solvent, and transfer of analytes to the MS
using probes.
Off-line analyte treatment process was time consuming and
there was an inherent risk of sample contamination.
Rapidly, it was realized that the analysis of complex
mixtures would require the development of a fully
automated on-line coupling solution in LC-MS.
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7. LIQUID CHROMATOGRAPHY
Liquid chromatography is a method of physical separation in
which the components of a liquid mixture are distributed between
two immiscible phases, i.e., stationary and mobile.
The practice of LC can be divided into five categories,
adsorption chromatography
partition chromatography
ion-exchange chromatography
size-exclusion chromatography
affinity chromatography
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8. Among these, the most widely used variant is the reverse-
phase (RP) mode of the partition chromatography
technique, which makes use of a non-polar (hydrophobic)
stationary phase and a polar mobile phase.
The mobile phase is a mixture of water and other polar
solvents
(e.g., methanol, isopropanol, and acetonitrile)
The stationary matrix is prepared by attaching long-chain
alkyl groups
(e.g., n-octadecyl or C18) to the surface of irregularly or
spherically shaped 5 μm diameter silica particles.
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9. In HPLC, typically 20 μl of the sample of interest are injected
into the mobile phase stream delivered by a high pressure pump.
The mobile phase containing the analytes permeates through the
stationary phase bed in a definite direction.
The components of the mixture are separated depending on their
chemical affinity with the mobile and stationary phases.
The liquid solvent (mobile phase) is delivered under high
pressure into a packed column containing the stationary phase.
The high pressure is necessary to achieve a constant flow rate for
reproducible chromatography experiments.
Depending on the partitioning between the mobile and stationary
phases, the components of the sample will flow out of the column
at different times.
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10. The column is the most important component of the LC system and
is designed to withstand the high pressure of the liquid.
Conventional LC columns are 100–300 mm long with outer
diameter of 6.4 mm (1/4 inch) and internal diameter of 3.0-4.6 mm.
For applications involving LC-MS, the length of chromatography
columns can be shorter (30–50 mm) with 3-5 μm diameter packing
particles.
In addition to the conventional model, other LC columns are the
narrowbore, microbore, microcapillary, and nano-LC models.
These columns have smaller internal diameters, allow for a more
efficient separation, and handle liquid flows under 1 ml/ min (the
conventional flow-rate).
In order to improve separation efficiency and peak resolution, ultra
performance liquid chromatography (UPLC) can be used instead of
HPLC.
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11. MASS SPECTROMETRY
Mass spectrometry (MS) is an analytical technique that
measures the mass-to-charge ratio (m/z) of charged particles
(ions).
Although there are many different kinds of mass
spectrometers, all of them make use of electric or magnetic
fields to manipulate the motion of ions produced from an
analyte of interest and determine their m/z.
The basic components of a mass spectrometer are the
Ion source
Mass analyzer
Detector
Data
Vacuum systems
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12. The ion source is where the components of a sample
introduced in a MS system are ionized by means of electron
beams, photon beams (UV lights), laser beams or corona
discharge.
In the case of electrospray ionization, the ion source moves
ions that exist in liquid solution into the gas phase.
The ion source converts and fragments the neutral sample
molecules into gas-phase ions that are sent to the mass
analyzer.
While the mass analyzer applies the electric and magnetic
fields to sort the ions by their masses, the detector
measures and amplifies the ion current to calculate the each
mass-resolved ion.
The data system records, processes, stores, and displays
data in a computer.
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13. The mass spectrum can be used to determine the mass of the analytes,
their elemental and isotopic composition, or to elucidate the chemical
structure of the sample.
MS is an experiment that must take place in gas phase and under
vacuum.
Therefore the development of devices facilitating the transition from
samples at higher pressure and in condensed phase (solid or liquid) into
a vacuum system has been essential to develop MS as a potent tool for
identification and quantification of organic compounds and peptides.
MS is now in very common use in analytical laboratories that study
physical, chemical, or biological properties of a great variety of
compounds.
Among the many different kinds of mass analyzers, the ones that find
application in LC-MS systems are the quadropole, time-of-flight
(TOF), ion traps, and hybrid quadropole-TOF (QTOF) analyzers.
9/3/2017 KMCH COLLEGE OF PHARMACY 13
15. Advantages disadvantages
simultaneous multianalyte analysis
Tends to have better sensitivity than GC
No derivitization needed
Have to over come the fear of LC
Expensive
Not portable
Requires an experienced technician
Only moderate throughput
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16. APPLICATIONS
The coupling of MS with LC systems is attractive because
liquid chromatography can separate delicate and complex
natural mixtures, which chemical composition needs to be
well established (e.g., biological fluids, environmental
samples, and drugs).
Nowadays, LC-MS has become one of the most widely used
chemical analysis techniques because more than 85% of
natural chemical compounds are polar and thermally labile
and GC-MS cannot process these samples.
As an example, HPLC-MS is regarded as the leading
analytical technique for proteomics and pharmaceutical
laboratories.
Other important applications of LC-MS include the analysis
of food, pesticides, and plant phenols.9/3/2017 KMCH COLLEGE OF PHARMACY 16
17. Pharmacokinetics
LC-MS is widely used in the field of bio-analysis and is
specially involved in pharmacokinetic studies of
pharmaceuticals.
Pharmacokinetic studies are needed to determine how
quickly a drug will be cleared from the body organs and the
hepatic blood flow.
MS analyzers are useful in these studies because of their
shorter analysis time, and higher sensitivity and specificity
compared to UV detectors commonly attached to HPLC
systems.
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18. Proteomics
LC-MS is used in proteomics as a method to detect and
identify the components of a complex mixture.
The bottom-up proteomics LC-MS approach generally
involves protease digestion and denaturation using trypsin
as a protease, urea to denature the tertiary structure, and
iodoacetamide to modify the cysteine residues.
After digestion, LC-MS is used for peptide mass
fingerprinting, or LC-MS/MS (tandem MS) is used to
derive sequence of individual peptides.
Samples of complex biological (e.g., human serum) may be
analyzed in modern LC-MS/MS systems, which can
identify over 1000 proteins. However, this high level of
protein identification is possible only after separating the
sample by means of SDS-PAGE gel.
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19. Metabolomics
LC-MS is also used for the analysis of natural products and
the profiling of secondary metabolites in plants.
LC-Nuclear magnetic resonance (NMR) is also used in
plant metabolomics, but this technique can only detect and
quantify the most abundant metabolites.
LC-MS has been useful to advance the field of plant
metabolomics, which aims to study the plant system at
molecular level.
The first application of LC-MS in plant metabolomics was
the detection of a wide range of highly polar metabolites,
oligosaccharides, amino acids, amino sugars, and sugar
nucleotides from Cucurbita maxima phloem tissues.
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20. Drug development
LC-MS is frequently used in drug development because it allows
quick molecular weight confirmation and structure identification.
LC-MS applications for drug development are highly automated
methods used for
Peptide mapping
Glycoprotein mapping
Natural products de-replication
Bio-affinity screening
In vivo drug screening
Metabolic stability screening
Metabolite identification
Impurity identification
Quality control
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