This document summarizes several types of molecular marker techniques used in PCR-based DNA fingerprinting: Random Amplified Polymorphic DNA (RAPD), Sequence-tagged Sites (STS), Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeats (SSR), Inter-Simple Sequence Repeats (ISSR), Sequence Characterized Amplified Regions (SCAR), and Cleaved Amplified Polymorphic Sequences (CAPS). It provides brief descriptions of each technique, including what type of DNA they use, how polymorphisms are detected, common primers and enzymes used, and some of their advantages and limitations.
Cancer therapies that target specific pathways can be more effective than established, nonspecific chemotherapy and radiation treatments, and may prevent side effects on healthy tissues. Such targeted therapies can only be applied after underlying gene mutations have been identified. However, detecting low frequency variants from clinically relevant samples poses significant challenges. Specimens are routinely formalin-fixed and paraffin-embedded (FFPE) for histology, which can decrease the efficiency of NGS library preparation. In this presentation, we discuss approaches for extraction of DNA from FFPE samples, and recommend quality control assays to guide parameter selection for library construction and sequencing depth.
High data quality and accuracy are recognized characteristics of Sanger re-sequencing projects and are primary reasons that next generation sequencing projects compliment their results by capillary electrophoresis data validation. We have developed an on-line tool called Primer Designer™ to streamline the NGS-to-Sanger sequencing workflow by taking the laborious task of PCR primer design out of the hands of the researcher by providing pre-designed assays for the human exome. The primer design tool has been created to enable scientists using next generation sequencing to quickly confirm variants discovered in their work by providing the means to quickly search, order and receive suitable pre-designed PCR primers for Sanger sequencing. Using the Primer Designer™ tool to design M13-tailed and non-tailed PCR primers for Sanger sequencing we will demonstrate validation of 28-variants across 24-amplicons and 19-genes using the BDD, BDTv1.1 and BDTv3.1 sequencing chemistries on the 3500xl Genetic Analyzer capillary electrophoresis platform.
Struggling with low editing efficiency or delivery problems? IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other methods. In this presentation we present the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explain why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. We also review RNP delivery using cationic lipids and electroporation, and provide tips for optimized transfection in your system.
End-point zygosity and CNV determination based on
post PCR Rn/ΔRn reading is expected to reduce the
cost and increase throughput as compared to current
real time Cq-based measurement. There is an
immediate need by AgBio customers to screen
seed zygosity and copy number of transgenes
through end-point reading. Moreover, Agbio customers
expect to determine zygosity and CNV using crude
samples in order to stream-line their workflow. For endpoint
quantification, the major challenge is to control
the saturation of PCR to maintain the segregation of
end-point intensity according to the input copy number
of the target transgenes in reference to a control gene.
We have tried a few approaches such as Asymmetric
PCR, ARCS (Amplification Ratio Control System)
PCR, Dual Tailing PCR, pyrophosphate removal and
controlling plateau of PCR by running a third PCR in
the background. Among the tested approaches,
controlled plateau of PCR (CoP’ed PCR) showed best
separation of copy number through end-point PCR
reading. We further optimized CoP’ed PCR conditions
using purified DNA and crude plant and blood samples.
By CoP’ed PCR, we are able to do zygosity with crude
plant samples to satisfy the immediate needs for Agbio
customers. Furthermore, since this approach improves
the sensitivity for the copy number separation for not
only end-point but also real-time PCR for crude blood
samples, in the future, it can be used for CNV analysis
directly from human blood.
Real-time quantitative PCR (qPCR) is a preferred platform for high throughput gene expression profiling, where large numbers of samples are characterized for hundreds of expression markers. Technically, the qPCR measurements are performed in the same way as when classical qPCR is used to analyze only a few targets per sample, but the higher throughput introduces additional sources of potential confounding variation that must be controlled for. In this presentation, Dr Kubista describes how high throughput qPCR profiling studies are designed. He covers assay optimization and validation, sample quality testing, and how to merge multi-plate measurements into a common analysis. Dr Kubista also discusses how to cost-effectively measure and compensate for background due to genomic DNA.
qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He demonstrates troubleshooting strategies. Experienced and novice users will benefit from an overview of uMeltSM software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.
Introduction to real-Time Quantitative PCR (qPCR) - Download the slidesQIAGEN
This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success.
Struggling with low editing efficiency or delivery problems in primary or difficult-to-transfect cells? In this presentation, learn about the advantages of using a Cas9:crRNA:tracrRNA ribonucleoprotein (RNP) complex for genome editing. We show the benefits of using RNP complexes, including ease of use, limiting off-target effects, and stability. We also present data showing how genome editing efficiency rates are improved by our Cas9 electroporation enhancer. Furthermore, we provide advice on how to optimize transfection using the Alt-R™ CRISPR-Cas9 System in combination with different electroporation methodologies.
[2020-09-01] IIBMP2020 Generating annotation texts of HLA sequences with anti...Eli Kaminuma
IIBMP2020 Poster #77 Generating annotation texts of HLA sequences with antigen classes by a T5 (Text-to-Text Transfer Transformer) model using International Nucleotide Sequence Database
Cancer therapies that target specific pathways can be more effective than established, nonspecific chemotherapy and radiation treatments, and may prevent side effects on healthy tissues. Such targeted therapies can only be applied after underlying gene mutations have been identified. However, detecting low frequency variants from clinically relevant samples poses significant challenges. Specimens are routinely formalin-fixed and paraffin-embedded (FFPE) for histology, which can decrease the efficiency of NGS library preparation. In this presentation, we discuss approaches for extraction of DNA from FFPE samples, and recommend quality control assays to guide parameter selection for library construction and sequencing depth.
High data quality and accuracy are recognized characteristics of Sanger re-sequencing projects and are primary reasons that next generation sequencing projects compliment their results by capillary electrophoresis data validation. We have developed an on-line tool called Primer Designer™ to streamline the NGS-to-Sanger sequencing workflow by taking the laborious task of PCR primer design out of the hands of the researcher by providing pre-designed assays for the human exome. The primer design tool has been created to enable scientists using next generation sequencing to quickly confirm variants discovered in their work by providing the means to quickly search, order and receive suitable pre-designed PCR primers for Sanger sequencing. Using the Primer Designer™ tool to design M13-tailed and non-tailed PCR primers for Sanger sequencing we will demonstrate validation of 28-variants across 24-amplicons and 19-genes using the BDD, BDTv1.1 and BDTv3.1 sequencing chemistries on the 3500xl Genetic Analyzer capillary electrophoresis platform.
Struggling with low editing efficiency or delivery problems? IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other methods. In this presentation we present the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explain why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. We also review RNP delivery using cationic lipids and electroporation, and provide tips for optimized transfection in your system.
End-point zygosity and CNV determination based on
post PCR Rn/ΔRn reading is expected to reduce the
cost and increase throughput as compared to current
real time Cq-based measurement. There is an
immediate need by AgBio customers to screen
seed zygosity and copy number of transgenes
through end-point reading. Moreover, Agbio customers
expect to determine zygosity and CNV using crude
samples in order to stream-line their workflow. For endpoint
quantification, the major challenge is to control
the saturation of PCR to maintain the segregation of
end-point intensity according to the input copy number
of the target transgenes in reference to a control gene.
We have tried a few approaches such as Asymmetric
PCR, ARCS (Amplification Ratio Control System)
PCR, Dual Tailing PCR, pyrophosphate removal and
controlling plateau of PCR by running a third PCR in
the background. Among the tested approaches,
controlled plateau of PCR (CoP’ed PCR) showed best
separation of copy number through end-point PCR
reading. We further optimized CoP’ed PCR conditions
using purified DNA and crude plant and blood samples.
By CoP’ed PCR, we are able to do zygosity with crude
plant samples to satisfy the immediate needs for Agbio
customers. Furthermore, since this approach improves
the sensitivity for the copy number separation for not
only end-point but also real-time PCR for crude blood
samples, in the future, it can be used for CNV analysis
directly from human blood.
Real-time quantitative PCR (qPCR) is a preferred platform for high throughput gene expression profiling, where large numbers of samples are characterized for hundreds of expression markers. Technically, the qPCR measurements are performed in the same way as when classical qPCR is used to analyze only a few targets per sample, but the higher throughput introduces additional sources of potential confounding variation that must be controlled for. In this presentation, Dr Kubista describes how high throughput qPCR profiling studies are designed. He covers assay optimization and validation, sample quality testing, and how to merge multi-plate measurements into a common analysis. Dr Kubista also discusses how to cost-effectively measure and compensate for background due to genomic DNA.
qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He demonstrates troubleshooting strategies. Experienced and novice users will benefit from an overview of uMeltSM software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.
Introduction to real-Time Quantitative PCR (qPCR) - Download the slidesQIAGEN
This slidedeck introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that are covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications and factors for success.
Struggling with low editing efficiency or delivery problems in primary or difficult-to-transfect cells? In this presentation, learn about the advantages of using a Cas9:crRNA:tracrRNA ribonucleoprotein (RNP) complex for genome editing. We show the benefits of using RNP complexes, including ease of use, limiting off-target effects, and stability. We also present data showing how genome editing efficiency rates are improved by our Cas9 electroporation enhancer. Furthermore, we provide advice on how to optimize transfection using the Alt-R™ CRISPR-Cas9 System in combination with different electroporation methodologies.
[2020-09-01] IIBMP2020 Generating annotation texts of HLA sequences with anti...Eli Kaminuma
IIBMP2020 Poster #77 Generating annotation texts of HLA sequences with antigen classes by a T5 (Text-to-Text Transfer Transformer) model using International Nucleotide Sequence Database
وصف الموضوع
من المؤكد و المعروف لدى الجميع أن هناك الكثير من الزيوت الطبيعية المفيدة للجسم , نذكر من بينها زيت الزنجبيل , فهو واحد من أهم الزيوت التي تمد الجسم بالعديد من الفوائد الطبيعية,
و قد تم استخدام الزنجبيل لقرون عديدة في علاج أمراض مختلفة ابتداءً من مشاكل التنفس إلى مشاكل الجهاز الهضمي , و يستخدم الزيت العطري لعلاج تلك الأمراض و غيرها الكثير .
رابط الموضوع
http://www.ar-only4men.com/home-remedy/benefits/ginger-oil.html
فوائد زيت الزيتون للشعر وطرق تحضير وصفات فعاله
http://www.ar-only4men.com/home-remedy/benefits/olive-oil-hair.html
فوائد زيت السمسم للجهاز التنفسى و وصفات للشعر والبشرة
http://www.ar-only4men.com/home-remedy/benefits/%d9%81%d9%88%d8%a7%d8%a6%d8%af-%d8%b2%d9%8a%d8%aa-%d8%a7%d9%84%d8%b3%d9%85%d8%b3%d9%85.html
8 وصفات طبيعية لعلاج الثعلبة بالثوم وزيت الخروع
http://www.ar-only4men.com/mens-health/dermatology/treatment-alopecia.html
فوائد زيت الزيتون للجسم والشعر والتنحيف
http://www.ar-only4men.com/home-remedy/benefits/%d9%81%d9%88%d8%a7%d8%a6%d8%af-
%d8%b2%d9%8a%d8%aa-%d8%a7%d9%84%d8%b2%d9%8a%d8%aa%d9%88%d9%86-
%d9%84%d9%84%d8%ac%d8%b3%d9%85-%d9%88%d8%a7%d9%84%d8%b4%d8%b9%d8%b1-%d9%88%d8%a7%d9%84%d8%aa%d9%86%d8%ad.html
Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be more challenging for lncRNAs than for mRNAs or miRNAs. In this presentation, we discuss some of the issues encountered with lncRNA research. We cover antisense oligonucleotide (ASO) and small interfering RNA (siRNA) methods for lncRNA knockdown. And, we show how cellular localization of a specific lncRNA target informs the choice of knockdown method.
An introduction to RNAi technology - Petr Svoboda - Institute of Molecular Ge...OECD Environment
10-12 April 2019: The OECD Conference on RNAi based pesticides provided an overview on the current status and future possibilities for the regulation of externally applied dsRNA-based products that are proposed for use as pesticides. The event facilitated exchanges between policy makers, academia, industry on their implications in health, environment, and regulation.
DNA polymerases (DNA manupliation Enzymes).pdfNetHelix
the Secrets of DNA Manipulation: A Comprehensive Exploration of DNA Polymerase and Enzymes
In this PDF presentation entitled "Enzymes that Manipulate DNA, Specially DNA Polymerase," we delve deep into the mechanisms and functions of these remarkable enzymes that play a pivotal role in the realm of molecular biology.
🧬 Key Highlights:
Introduction to DNA Polymerase:
Uncover the fundamental aspects of DNA polymerase, a key player in DNA replication and repair. Explore its structure, functions, and the indispensable role it plays in maintaining the genetic integrity of living organisms.
Types of DNA Polymerases:
Delve into the diverse landscape of DNA polymerases, ranging from prokaryotic to eukaryotic systems. Understand how different types of DNA polymerases contribute to the precision and efficiency of DNA synthesis.
Examples of polymerases:
•DNA polymerase 1
•klenow fragment
•sequenase
•Taq polymerase
•Reverse Transcriptase
DNA Replication
Take a closer look at the intricate dance of enzymes during DNA replication. Follow the step-by-step process, and gain insights into how DNA polymerase ensures the accurate transmission of genetic information from one generation to the next.
Technological Applications:
Unleash the potential of DNA polymerase in various biotechnological applications. From PCR (Polymerase Chain Reaction) to DNA sequencing, discover how these enzymes have revolutionized molecular biology and genetic research.
Emerging Trends and Future Prospects:
Stay ahead of the curve by exploring the latest advancements and emerging trends in DNA manipulation. Witness the ongoing research that promises to unlock new possibilities in the field.
🎓 Who Should Explore This Presentation?
Students and researchers in molecular biology and genetics
Biotechnologists and professionals in the field of genetic engineering
Enthusiasts curious about the molecular machinery behind DNA manipulation
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
9. 11--Simple Sequence Repeats or MicrosatellitesSimple Sequence Repeats or Microsatellites ((SSRSSR(:(:
1 2 3 41 2 3 4
ATC ATC ATC ATCATC ATC ATC ATC
Primer 2Primer 2Primer 1Primer 1
SSRSSR22––33
SSR primerSSR primer::
Primer name Right primer Left primer
Xgwm311-2A CTA CGT GCA CCA CCA TTT TG TCA CGT GGA AGA CGC TCC
