Molecular markers and plant breeding3

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كلية الزراعة جامعة الاسكندرية

Molecular

Education

DNADNA DNA Extraction or IsolationDNA Extraction or Isolation::
DNA markersDNA markers..
DNADNA
DNADNA

DNADNA
11
Sodium dodecycl sulphate (SDSSodium dodecycl sulphate (SDS((..
CTABCTAB..MTABMTAB..
Tris / HClTris / HClpHpH77
22DNADNAEndogenous nucleasesEndogenous nucleases
EDTAEDTAExtraction bufferExtraction buffer
DNADNA..

.(.(
6565
proteinase-Kproteinase-K..
33
44RNARNA
RNARNARNaseRNase..
55NaClNaClKClKCl ++ CTABCTAB..

55XX
RFLPRFLP::
PCRPCR::
11DNADNA..
33
RFLPRFLP((Restriction Fragment Length PolymorphismRestriction Fragment Length Polymorphism((
22--Restriction of DNARestriction of DNA..
44--Screening by probeScreening by probe..

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