Does your next experiment involve Cathepsin B (CTSB)? This is a presentation about CTSB intended for scientists who are designing controls and performing immunoassays detecting CTSB. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (M01456): https://www.bosterbio.com/anti-cathepsin-b-rabbit-monoclonal-antibody-m01456-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6351122, PMC4091172, PMC7391525 Learn more about CTSB (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/CTSB Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

1. www.bosterbio.com
CTSB IN 3 MINUTES
Quick facts, control designs, Western Blot MW.
Info you can use to find the best CTSB antibody.

2. FACTS
01.
Function, structure, etc.
TISSUES
02.
Expressing tissues/cells
WESTERN BLOT
03.
Expected band sizes
HISTORY
04.
…and medical significance
TABLE OF CONTENTS

3. ● Cathepsin B (CTSB)
● Bilobal protein
○ Catalytic site is located at
interface between the 2 lobes
● Precursor/pro-form
○ 339 AAs, ~35 kDa
● Mature form
○ 254 AAs, ~25 kDa
01. FACTS
AlphaFold predicted structure
Image source: https://alphafold.ebi.ac.uk/entry/P07858

4. 02. TISSUES
Image source: https://www.proteinatlas.org/ENSG00000164733-CTSB/tissue

5. 03. CTSB WESTERN BLOT
PMC6351122 Figure 1
PMC4091172 Figure 4
Boster Bio CTSB
Antibody (M01456,
Rabbit monoclonal)
161 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=ctsb+western+blot&report=imagesdocsum
After reviewing several WB images, we observe bands of Pro CTSB at ~35 kDa and
bands of mature CTSB at ~25 kDa
.
PMC7391525 Figure 4
*Mature form not present

6. Subcellular fractionation of
melanoma cells revealed the
presence of single chain form of
CTSB on the plasma membrane.
04. HISTORY
CTSB is of significant importance
to cancer therapy because of its
involvement with oncogenic
processes in humans.
1986 CLINICAL SIGNIFICANCE

7. 3942 B Valley Ave
Pleasanton, CA 94566
Tel: (888) 466-3604
Email: support@bosterbio.com
www.bosterbio.com