Does your next experiment involve Interferon gamma? This is a presentation about IFN Gamma/IFNG intended for scientists who are designing controls and performing immunoassays detecting IFN Gamma/IFNG. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Interferon Gamma IFNG Rabbit Monoclonal Antibody (M00393-1): https://www.bosterbio.com/anti-interferon-gamma-rabbit-monoclonal-antibody-m00393-1-boster.html Anti-IFN Gamma Antibody (RP1001): https://www.bosterbio.com/anti-rat-ifn-gamma-antibody-rp1001-boster.html References: Uniprot.org, ProteinAtlas.org, PMID15220936, PMID9524237, PMID24253448, PMC7086207 Learn more about IFN Gamma/IFNG (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IFNG Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

1. www.bosterbio.com
IFN-γ IN 3 MINUTES
Quick facts, control designs, Western Blot MW.
Info you can use to find the best IFN-γ antibody.

2. FACTS
01.
Function, structure, etc.
TISSUES
02.
Expressing tissues/cells
WESTERN BLOT
03.
Expected band sizes
HISTORY
04.
…and medical significance
TABLE OF CONTENTS

3. ● Interferon Gamma (IFN-γ)
● Cytokine family
● Produced by NK cells and Th1 cells
● Biologically active form is a dimer
● Precursor/pro-form
○ 166 amino acids long
○ Gets cleaved into mature IFN-γ
for activation
● Mature form
○ 138 amino acids long
○ ~23 kDa
01. FACTS
AlphaFold predicted structure
Image source: https://alphafold.ebi.ac.uk/entry/P01579

4. 02. TISSUES
Image source: https://www.proteinatlas.org/ENSG00000111537-IFNG/tissue

5. 03. IFN-γ WESTERN BLOT
3252 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=ifn-
gamma+western+blot&report=imagesdocsum
After reviewing several WB images, we observe mature IFN-γ at ~17-25 kDa.
PMID24253448
Figure 1
Boster Anti-IFN-γ
Rabbit Monoclonal
Antibody (M00393-1)
PMID15220936 Figure 5
PMC7086207 Figure 3
Boster Anti-IFN
Gamma Antibody
(RP1001)
PMID9524237 Figure 6

6. Promotes antiproliferative activity
against cancer
04. HISTORY
Helps remodel the arteries in the
uterus to allow the growth of
placenta
ANTI-CANCER PREGNANCY INITIATION

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