Does your next experiment involve NGFβ? This is a presentation about NGF/NGF Beta intended for scientists who are designing controls and performing immunoassays detecting NGF/beta-NGF. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-NGF/NGF Beta Antibody Picoband™ (A00341): https://www.bosterbio.com/anti-ngf-ngf-beta-picoband-trade-antibody-a00341-boster.html References: Uniprot.org, ProteinAtlas.org, GeneCards, PMC4760111, PMC6037903, PMC2650228, PMC1894684 Learn more about NGF/NGF Beta (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/NGF Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

1. www.bosterbio.com
NGFβ IN 3 MINUTES
Quick facts, control designs, Western Blot MW.
Info you can use to find the best NGFβ antibody.

2. FACTS
01.
Function, structure, etc.
TISSUES
02.
Expressing tissues/cells
WESTERN BLOT
03.
Expected band sizes
HISTORY
04.
…and medical significance
TABLE OF CONTENTS

3. ● Beta-nerve growth factor (NGF,
NGF Beta, NGFβ)
● Important for the development and
maintenance of the sympathetic
and sensory nervous systems
● Homodimer protein
● Mature NGFβ
○ 241 amino acids long
○ MW: 26.959 kDa
● proNGF: ~130 kDa
01. FACTS
AlphaFold predicted structure
Image source: https://alphafold.ebi.ac.uk/entry/P01138

4. 02. TISSUES
Image source: https://www.proteinatlas.org/ENSG00000134259-NGF/tissue

5. 1230 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=ngf+western+blot&report=imagesdocsum
After reviewing several WB images, we observe mature NGF Beta monomer at ~13 kDa.
03. NGFβ WESTERN BLOT
PMC4760111 Figure
2
Boster Anti-NGF
Antibody (A00341)
PMC2650228 Figure 2
PMC1894684 Figure 1
PMC6037903 Figure 1

6. NGFβ is a neurotrophic factor and
a neuropeptide that regulates the
growth, maintenance, proliferation,
and survival of specific neurons.
04. HISTORY
Mutations in NGF have been linked
with neuropathy, particularly
hereditary sensory and autonomic
neuropathy (HSAN).
1950s CLINICAL POTENTIAL

7. 3942 B Valley Ave
Pleasanton, CA 94566
Tel: (888) 466-3604
Email: support@bosterbio.com
www.bosterbio.com