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Learn ITGA3 in 3 minutes | Integrin alpha-3
The document provides quick facts about integrin alpha-3 (itga3), including its function, structure, and clinical significance. It is a receptor for proteins like fibronectin and collagen, with a length of 1,051 amino acids and a molecular weight of approximately 116 kDa. Additionally, it discusses the potential clinical implications of itga3 expression in diseases such as congenital nephrotic syndrome and breast cancer prognosis.
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Learn ITGA3 in 3 minutes | Integrin alpha-3
- 1. www.bosterbio.com ITGA3 IN 3MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best ITGA3 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressingtissues/cells WESTERN BLOT 03. Expected band sizes SIGNIFICANCE 04. Clinical potential TABLE OF CONTENTS
- 3. ● Integrin alpha-3(ITGA3) ● Belongs to the integrin family ● Receptor for proteins such as fibronectin, laminin, and collagen ● ITGA3 is 1,051 amino acids long ● Protein size: ~116 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P26006
- 4. 02. TISSUES Image source:https://www.proteinatlas.org/ENSG00000005884-ITGA3/tissue
- 5. 03. ITGA3 WESTERNBLOT 33 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=ITGA3+western+blot&report=imagesdocsum After reviewing several WB images, we observe ITGA3 at ~116 kDa. PMC2947217 Figure 4 PMC6034741 Figure 4 Boster Bio ITGA3 Antibody (PA1621, Rabbit Polyclonal) PMC7327512 Figure 6
- 6. ITGA3 mutations areassociated with congenital nephrotic syndrome, interstitial lung disease, and epidermolysis bullosa. 04. SIGNIFICANCE Breast cancer patients with higher expression of ITGA3 had an improved prognosis. DISEASE CLINICAL POTENTIAL
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Editor's Notes
- #2 Does your next experiment involve ITGA3? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against ITGA3.
- #3 We will cover some basic facts, protein expression, and ITGA3’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- #4 Topic 1: Quick facts about ITGA3. Integrin alpha 3, or ITGA3, belongs to the integrin family and is a receptor for important proteins such as fibronectin, laminin, and collagen. ITGA3 is 1,051 amino acids long and runs about 116 kilodaltons on a western blot.
- #5 Topic 2: Where is ITGA3? ITGA3 is expressed in most tissues, such as the respiratory system, endocrine tissues, kidney, urinary bladder, gastrointestinal tract, skin, and other tissues. You can find more information on protein atlas dot org to check out which cell lines express ITGA3 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- #6 Topic 3: ITGA3 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a ITGA3 western blot at about 116 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- #7 Topic 4: Some interesting facts about ITGA3. Mutations to ITGA3 have so far been associated with congenital nephrotic syndrome, interstitial lung disease, and epidermolysis bullosa in patients. Interestingly, breast cancer patients with higher expression of ITGA3 had an improved prognosis.