Learn CD133 in 3 minutes | Prominin-1, PROM1

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Does your next experiment involve CD133 (Prominin-1)? This is a presentation about CD133/Prominin-1/PROM1 intended for scientists who are designing controls and performing immunoassays detecting Prominin-1. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-PROM1 Antibody Picoband™ (PB9156): https://www.bosterbio.com/anti-prom1-picoband-trade-antibody-pb9156-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6763460, PMC2713505, PMC6030467 Learn more about CD133/Prominin-1/PROM1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/PROM1 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

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CD133 IN 3 MINUTES
Quick facts, control designs, Western Blot MW.
Info you can use to find the best CD133 antibody.

FACTS
01.
Function, structure, etc.
TISSUES
02.
Expressing tissues/cells
WESTERN BLOT
03.
Expected band sizes
SIGNIFICANCE
04.
Clinical potential
TABLE OF CONTENTS

● CD133
○ also known as Prominin-1
● Membrane glycoprotein
● Stem cell biomarker
● Cleaved into:
○ 19-amino-acid long signal peptide
○ mature CD133
(846 amino acids long)
● Western blot: ~97-130 kDa
01. FACTS
AlphaFold predicted structure
Image source: https://alphafold.ebi.ac.uk/entry/O43490

02. TISSUES
Image source: https://www.proteinatlas.org/ENSG00000007062-PROM1/tissue

03. CD133 WESTERN BLOT
1219 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=cd133+western+blot&report=imagesdocsum
After reviewing several WB images, we observe CD133 at ~97-130 kDa.
Boster Bio CD133
Antibody (PB9156,
Rabbit Polyclonal)
PMC2713505 Figure 3
PMC6763460 Figure 1
PMC6030467 Figure 1

CD133 is a stem cell biomarker.
It is also found in cancer stem cells
(CSCs) or tumor-initiating cells.
04. SIGNIFICANCE
CD133 is a membrane glycoprotein
used to separate stem cells from a
mixture of cells in a tissue.
STEM CELL
BIOMARKER
STEM CELL
ISOLATION

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Does your next experiment involve Insulin-like growth factor I (IGF-I, IGF-1)? This is a presentation about IGF1 intended for scientists who are designing controls and performing immunoassays detecting IGF1. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-IGF1 Mouse Monoclonal Antibody (M00148-1): https://www.bosterbio.com/anti-igf1-mouse-monoclonal-antibody-clone-id-oti4b12-m00148-1-boster.html References: Uniprot.org, ProteinAtlas.org, PMC5867890, PMC7450330, PMC7262660, PMC7401641 Learn more about IGF1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IGF1 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

Learn LBP in 3 minutes | Lipopolysaccharide binding protein

Boster Biological Technology

Does your next experiment involve Lipopolysaccharide binding protein (LBP)? This is a presentation about LBP intended for scientists who are designing controls and performing immunoassays detecting LBP. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-LBP Antibody Picoband™ (A00809-1): https://www.bosterbio.com/anti-lbp-picoband-trade-antibody-a00809-1-boster.html References: Uniprot.org, ProteinAtlas.org, PMC3617275, PMC3485520, PMC6945416, PMC8710911 Learn more about LBP (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/LBP Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

Learn NEFH/NF-H in 3 minutes | Neurofilament Heavy Chain

Boster Biological Technology

Does your next experiment involve Neurofilament Heavy Polypeptide (NEFH)? This is a presentation about NEFH/NF-H intended for scientists who are designing controls and performing immunoassays detecting NEFH/NF-H. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-NEFH/Nf H Rabbit Monoclonal Antibody (M05307-2): https://www.bosterbio.com/anti-nefh-rabbit-monoclonal-antibody-m05307-2-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6834541, PMC6054723, PMC4285109 Learn more about NEFH/NF-H (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/NEFH Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

Learn LCN2/NGAL in 3 minutes | Lipocalin-2

Boster Biological Technology

This document provides key information about the protein Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL). It discusses LCN2's function, structure, expressing tissues/cells, expected band size on a Western Blot, role in the innate immune response, and clinical significance in acute kidney injury. The document includes diagrams of LCN2's predicted structure and expressing tissues. It also references multiple published Western Blots demonstrating that LCN2 appears at around 25 kDa.

Learn IL-1 Beta in 3 minutes | Interleukin-1 beta, IL1B, IL-1F2

Boster Biological Technology

Does your next experiment involve interleukin 1 beta? This is a presentation about IL-1 Beta/IL-1F2/IL1B intended for scientists who are designing controls and performing immunoassays detecting IL-1B. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-IL1 Beta/IL1B Antibody (PA1341): https://www.bosterbio.com/anti-il-1-beta-antibody-pa1341-boster.html References: Uniprot.org, ProteinAtlas.org, PMC3507778, PMC6544845, PMC8491766, PMC7652828, PMC6854078 Learn more about IL-1 Beta/IL-1F2/IL1B (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IL1B Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com

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Authoring a personal GPT for your research and practice: How we created the Q...

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Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.

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As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus, high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology, and analysis methods, numerous infrastructure platforms have been developed for phenotyping.

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Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.

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如何办理(uvic毕业证书)维多利亚大学毕业证本科学位证书原版一模一样

yqqaatn0

原版纸张【微信：741003700 】【(uvic毕业证书)维多利亚大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma 1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700 留信网认证的作用: 1:该专业认证可证明留学生真实身份 2:同时对留学生所学专业登记给予评定 3:国家专业人才认证中心颁发入库证书 4:这个认证书并且可以归档倒地方 5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息 6:个人职称评审加20分 7:个人信誉贷款加10分 8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才

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mô tả các thí nghiệm về đánh giá tác động dòng khí hóa sau đốt

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With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.

Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...

AbdullaAlAsif1

The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.

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Classification Metal ion ion indicators Masking and demasking reagents Estimation of Magnisium sulphate Calcium gluconate Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types- 1.Direct Titration 2.Back Titration 3.Replacement Titration 4.Indirect Titration Masking agent, Demasking agents formation of complex comparition between masking and demasking agents, Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators, Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators Instrumental Techniques-Photometry Potentiometry Miscellaneous methods. Complex titration with EDTA.

EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...

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Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions among stars. Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars. The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun. Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically, the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec. Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation were carried out using the ACIS-Extract software. Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a photon flux threshold of approximately 2 × 10−8 photons cm−2 s −1 . The X-ray sources exhibit a highly concentrated spatial distribution, with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.

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Learn CD133 in 3 minutes | Prominin-1, PROM1

1. www.bosterbio.com CD133 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best CD133 antibody.

2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes SIGNIFICANCE 04. Clinical potential TABLE OF CONTENTS

3. ● CD133 ○ also known as Prominin-1 ● Membrane glycoprotein ● Stem cell biomarker ● Cleaved into: ○ 19-amino-acid long signal peptide ○ mature CD133 (846 amino acids long) ● Western blot: ~97-130 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/O43490

4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000007062-PROM1/tissue

5. 03. CD133 WESTERN BLOT 1219 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=cd133+western+blot&report=imagesdocsum After reviewing several WB images, we observe CD133 at ~97-130 kDa. Boster Bio CD133 Antibody (PB9156, Rabbit Polyclonal) PMC2713505 Figure 3 PMC6763460 Figure 1 PMC6030467 Figure 1

6. CD133 is a stem cell biomarker. It is also found in cancer stem cells (CSCs) or tumor-initiating cells. 04. SIGNIFICANCE CD133 is a membrane glycoprotein used to separate stem cells from a mixture of cells in a tissue. STEM CELL BIOMARKER STEM CELL ISOLATION

7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com

Editor's Notes

Does your next experiment involve CD133? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against CD133.
We will cover some basic facts, protein expression, and CD133’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
Topic 1: Quick facts about CD133. Also known as Prominin-1, CD133 is part of the prominin family of proteins. It is a membrane glycoprotein that serves as a biomarker for stem cells. The protein consists of a 19-amino-acid long signal peptide that is cleaved off to generate the mature CD133 which is 846 amino acids long. CD133 runs about 97 to 130 kilodaltons on Western blot.
Topic 2: Where is CD133 expressed? High levels of CD133 have been found in the respiratory system, the renal system, female reproductive organs, and the hepatic tissues. High RNA expression has been reported for the retina, but there is limited protein expression. CD133 is also expressed at lower levels in the endocrine tissues, male reproductive tissues, skin, bone marrow, pancreas, and muscles. You can find more information at protein atlas dot org including which cell lines express CD133 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
Topic 3: CD133 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically a band ranging from 97 to 130 kilodaltons can be expected in a CD133 Western blot. Check out the PMC link for more images, which is also provided in the description box.
Topic 4: Some interesting facts about CD133. Prominin-1 is a membrane glycoprotein that is used to identify stem cells or stem-like cells. It has been extensively used to isolate stem cells from pathological tissue mixtures. Furthermore, scientists are using CD133 to identify and target cancer stem cells. This biomarker holds significant therapeutic potential, which has been demonstrated in recent studies involving metastatic melanomas. In the future, we may see more research development related to cancer stem cell targeting using CD133.

Learn CD133 in 3 minutes | Prominin-1, PROM1

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