The document summarizes an experiment on the effects of substrate concentration, temperature, and pH on an enzyme reaction. It finds that a lower Km value indicates a higher affinity of the enzyme for the substrate. It also determines that increasing temperature increases the rate of reaction up to a point, and that most enzymes function best within a specific pH range, with activity lost outside this range due to breaking of bonds in the enzyme structure. The experiment is able to prepare standard reference graphs and investigate how the three factors influence the enzyme concentration as measured by the maximum reaction rate Vmax and Michaelis constant Km.