enzyme kinetics.pptx
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The key factors that affect enzyme activity are the concentration of the enzyme and substrate, temperature, pH, product concentration, and presence of activators. The maximum velocity of the reaction increases with higher enzyme and substrate concentrations up to a point. Temperature and pH also affect activity, with optimal rates usually around body temperature and neutral pH. Products can inhibit the enzyme as they accumulate. Certain metals are required as cofactors for some enzymes to function properly.
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ENZYME INHIBITION & FACTORS AFFECTING THE VELOCITY OF ENZYME ACTION
This document discusses several key factors that affect enzyme activity:
1. Enzyme and substrate concentration - Reaction rate increases with increasing concentrations of enzyme and substrate up to a maximum.
2. Temperature and pH - Enzymes have optimal temperatures and pH levels for activity. Outside these ranges, activity decreases.
3. Inhibitors and activators - Substances that bind enzymes can inhibit or activate their activity, altering reaction rates. Common types of inhibitors are competitive, non-competitive, and uncompetitive.
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The document discusses several key factors that affect enzyme activity: enzyme concentration, substrate concentration, temperature, pH, product concentration, presence of activators, time, and light/radiation. It explains how increasing or decreasing each factor can increase or decrease the reaction rate. For example, increasing enzyme concentration or an optimal temperature can increase the reaction rate, while too high or low of a pH can decrease it. The Michaelis-Menten equation and Lineweaver-Burk plots are also introduced to model enzyme kinetics.
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This document summarizes key concepts about enzyme kinetics and regulation:
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2) Values like Km, Vmax, and Kcat/Km characterize enzyme-substrate binding affinity and catalytic efficiency. Km is the substrate concentration when V0 is half Vmax.
3) Reactions can involve single or multiple substrates through sequential or ping-pong mechanisms. Allosteric enzymes have cooperative binding between subunits and may be regulated by pathway end-products.
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This document discusses factors that affect enzyme activity. It notes that enzymes are proteins that catalyze reactions by reducing activation energy. Several factors influence enzyme reaction velocity, including the concentrations of the enzyme and substrate, pH, temperature, and presence of inhibitors or activators. The Michaelis-Menten constant (Km) characterizes the strength of binding between an enzyme and its substrate. Enzyme activity typically increases with substrate concentration up to a maximum, follows a bell curve with temperature, and has an optimal pH value. Certain metals can activate enzymes by participating in reactions or inducing conformational changes.
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1) The document discusses enzyme kinetics and the Michaelis-Menten model of enzyme kinetics.
2) It introduces key concepts such as the Michaelis constant Km and maximum velocity Vmax, and derives the Michaelis-Menten equation relating substrate concentration, reaction rate, Km and Vmax.
3) It also discusses factors that affect enzyme activity such as temperature, pH, and enzyme inhibitors, distinguishing between competitive, uncompetitive, and noncompetitive inhibition.
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2. It also covers enzyme inhibition, describing reversible inhibition as competitive or non-competitive, and irreversible inhibition.
3. Key concepts explained are Michaelis-Menten kinetics, the Michaelis constant Km, Lineweaver-Burk plots, and the effects of various factors on the reaction rate.
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This document discusses several key factors that affect enzyme activity:
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2. Temperature and pH - Enzymes have optimal temperatures and pH levels for activity. Outside these ranges, activity decreases.
3. Inhibitors and activators - Substances that bind enzymes can inhibit or activate their activity, altering reaction rates. Common types of inhibitors are competitive, non-competitive, and uncompetitive.
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The document discusses several key factors that affect enzyme activity: enzyme concentration, substrate concentration, temperature, pH, product concentration, presence of activators, time, and light/radiation. It explains how increasing or decreasing each factor can increase or decrease the reaction rate. For example, increasing enzyme concentration or an optimal temperature can increase the reaction rate, while too high or low of a pH can decrease it. The Michaelis-Menten equation and Lineweaver-Burk plots are also introduced to model enzyme kinetics.
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The document discusses factors that affect the rate of enzyme action, including enzyme concentration, substrate concentration, temperature, pH, concentration of coenzymes and activators, time, and inhibitors. It provides details on how each factor influences the reaction rate. Specifically, it explains that enzyme activity is highest when substrate concentration is saturating but not excessive, and when other conditions like temperature and pH are optimal. The document also describes Michaelis-Menten kinetics and how reversible and irreversible inhibition can decrease reaction rates.
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- Enzymes can be classified based on the type of reaction catalyzed according to the IUB system into 6 major classes including oxidoreductases and hydrolases.
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#NUTRITIONIST #INTERNATIONALDIETICIANS
This content is made for all student of medical ,nutrition ,doctors ,zoology ,chemistry ,medical who are still preparing for examination .feel free to give suggestion.
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2) Enzymes can be inhibited through competitive, mixed, and allosteric inhibition mechanisms which impact the Michaelis-Menten curve.
3) Assays of enzyme activity can be used as diagnostic tools to detect tissue damage or disease based on abnormal levels of enzymes detected in blood serum.
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2) The Michaelis-Menten equation models reaction rates as substrate concentration increases, with an initial linear increase until reaching the maximum reaction rate (Vmax) at substrate saturation.
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JAMES WEBB STUDY THE MASSIVE BLACK HOLE SEEDS
The pathway(s) to seeding the massive black holes (MBHs) that exist at the heart of galaxies in the present and distant Universe remains an unsolved problem. Here we categorise, describe and quantitatively discuss the formation pathways of both light and heavy seeds. We emphasise that the most recent computational models suggest that rather than a bimodal-like mass spectrum between light and heavy seeds with light at one end and heavy at the other that instead a continuum exists. Light seeds being more ubiquitous and the heavier seeds becoming less and less abundant due the rarer environmental conditions required for their formation. We therefore examine the different mechanisms that give rise to different seed mass spectrums. We show how and why the mechanisms that produce the heaviest seeds are also among the rarest events in the Universe and are hence extremely unlikely to be the seeds for the vast majority of the MBH population. We quantify, within the limits of the current large uncertainties in the seeding processes, the expected number densities of the seed mass spectrum. We argue that light seeds must be at least 103 to 105 times more numerous than heavy seeds to explain the MBH population as a whole. Based on our current understanding of the seed population this makes heavy seeds (Mseed > 103 M⊙) a significantly more likely pathway given that heavy seeds have an abundance pattern than is close to and likely in excess of 10−4 compared to light seeds. Finally, we examine the current state-of-the-art in numerical calculations and recent observations and plot a path forward for near-future advances in both domains.
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Post translation modification by Suyash Garg
overview of PTM helps to the students who wants to clear their basics about it.
Evaluation and Identification of J'BaFofi the Giant Spider of Congo and Moke...
ABSTRACT
The J'BaFofi, or "Giant Spider," is a mainly legendary arachnid by reportedly inhabiting the dense rain forests of
the Congo. As despite numerous anecdotal accounts and cultural references, the scientific validation remains more elusive.
My study aims to proper evaluate the existence of the J'BaFofi through the analysis of historical reports,indigenous
testimonies and modern exploration efforts.
Rodents, Birds and locust_Pests of crops.pdf
Mole rat or Lesser bandicoot rat, Bandicotabengalensis
•Head -round and broad muzzle
•Tail -shorter than head, body
•Prefers damp areas
•Burrows with scooped soil before entrance
•Potential rat, one pair can produce more than 800 offspringsin one year
SDSS1335+0728: The awakening of a ∼ 106M⊙ black hole⋆
Context. The early-type galaxy SDSS J133519.91+072807.4 (hereafter SDSS1335+0728), which had exhibited no prior optical variations during the preceding two decades, began showing significant nuclear variability in the Zwicky Transient Facility (ZTF) alert stream from December 2019 (as ZTF19acnskyy). This variability behaviour, coupled with the host-galaxy properties, suggests that SDSS1335+0728 hosts a ∼ 106M⊙ black hole (BH) that is currently in the process of ‘turning on’. Aims. We present a multi-wavelength photometric analysis and spectroscopic follow-up performed with the aim of better understanding the origin of the nuclear variations detected in SDSS1335+0728. Methods. We used archival photometry (from WISE, 2MASS, SDSS, GALEX, eROSITA) and spectroscopic data (from SDSS and LAMOST) to study the state of SDSS1335+0728 prior to December 2019, and new observations from Swift, SOAR/Goodman, VLT/X-shooter, and Keck/LRIS taken after its turn-on to characterise its current state. We analysed the variability of SDSS1335+0728 in the X-ray/UV/optical/mid-infrared range, modelled its spectral energy distribution prior to and after December 2019, and studied the evolution of its UV/optical spectra. Results. From our multi-wavelength photometric analysis, we find that: (a) since 2021, the UV flux (from Swift/UVOT observations) is four times brighter than the flux reported by GALEX in 2004; (b) since June 2022, the mid-infrared flux has risen more than two times, and the W1−W2 WISE colour has become redder; and (c) since February 2024, the source has begun showing X-ray emission. From our spectroscopic follow-up, we see that (i) the narrow emission line ratios are now consistent with a more energetic ionising continuum; (ii) broad emission lines are not detected; and (iii) the [OIII] line increased its flux ∼ 3.6 years after the first ZTF alert, which implies a relatively compact narrow-line-emitting region. Conclusions. We conclude that the variations observed in SDSS1335+0728 could be either explained by a ∼ 106M⊙ AGN that is just turning on or by an exotic tidal disruption event (TDE). If the former is true, SDSS1335+0728 is one of the strongest cases of an AGNobserved in the process of activating. If the latter were found to be the case, it would correspond to the longest and faintest TDE ever observed (or another class of still unknown nuclear transient). Future observations of SDSS1335+0728 are crucial to further understand its behaviour. Key words. galaxies: active– accretion, accretion discs– galaxies: individual: SDSS J133519.91+072807.4
Mites,Slug,Snail_Infesting agricultural crops.pdf
Order : Trombidiformes (Acarina) Class : Arachnida
Mites normally feed on the undersurface of the leaves but the symptoms are more easily seen on the uppersurface.
Tetranychids produce blotching (Spots) on the leaf-surface.
Tarsonemids and Eriophyids produce distortion (twist), puckering (Folds) or stunting (Short) of leaves.
Eriophyids produce distinct galls or blisters (fluid-filled sac in the outer layer)
BIRDS DIVERSITY OF SOOTEA BISWANATH ASSAM.ppt.pptx
Ahota Beel, nestled in Sootea Biswanath Assam , is celebrated for its extraordinary diversity of bird species. This wetland sanctuary supports a myriad of avian residents and migrants alike. Visitors can admire the elegant flights of migratory species such as the Northern Pintail and Eurasian Wigeon, alongside resident birds including the Asian Openbill and Pheasant-tailed Jacana. With its tranquil scenery and varied habitats, Ahota Beel offers a perfect haven for birdwatchers to appreciate and study the vibrant birdlife that thrives in this natural refuge.
一比一原版美国佩斯大学毕业证如何办理
原版一模一样【微信：741003700 】【美国佩斯大学毕业证成绩单】【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
办理美国佩斯大学毕业证【微信：741003700 】外观非常简单，由纸质材料制成，上面印有校徽、校名、毕业生姓名、专业等信息。
办理美国佩斯大学毕业证【微信：741003700 】格式相对统一，各专业都有相应的模板。通常包括以下部分：
校徽：象征着学校的荣誉和传承。
校名:学校英文全称
授予学位：本部分将注明获得的具体学位名称。
毕业生姓名：这是最重要的信息之一，标志着该证书是由特定人员获得的。
颁发日期：这是毕业正式生效的时间，也代表着毕业生学业的结束。
其他信息：根据不同的专业和学位，可能会有一些特定的信息或章节。
办理美国佩斯大学毕业证【微信：741003700 】价值很高，需要妥善保管。一般来说，应放置在安全、干燥、防潮的地方，避免长时间暴露在阳光下。如需使用，最好使用复印件而不是原件，以免丢失。
综上所述，办理美国佩斯大学毕业证【微信：741003700 】是证明身份和学历的高价值文件。外观简单庄重，格式统一，包括重要的个人信息和发布日期。对持有人来说，妥善保管是非常重要的。
Embracing Deep Variability For Reproducibility and Replicability
Embracing Deep Variability For Reproducibility and ReplicabilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
Embracing Deep Variability For Reproducibility and Replicability
Abstract: Reproducibility (aka determinism in some cases) constitutes a fundamental aspect in various fields of computer science, such as floating-point computations in numerical analysis and simulation, concurrency models in parallelism, reproducible builds for third parties integration and packaging, and containerization for execution environments. These concepts, while pervasive across diverse concerns, often exhibit intricate inter-dependencies, making it challenging to achieve a comprehensive understanding. In this short and vision paper we delve into the application of software engineering techniques, specifically variability management, to systematically identify and explicit points of variability that may give rise to reproducibility issues (eg language, libraries, compiler, virtual machine, OS, environment variables, etc). The primary objectives are: i) gaining insights into the variability layers and their possible interactions, ii) capturing and documenting configurations for the sake of reproducibility, and iii) exploring diverse configurations to replicate, and hence validate and ensure the robustness of results. By adopting these methodologies, we aim to address the complexities associated with reproducibility and replicability in modern software systems and environments, facilitating a more comprehensive and nuanced perspective on these critical aspects.
https://hal.science/hal-04582287
Noida Call Girls Number 9999965857 Vip Call Girls Lady Of Your Dream Ready To...
Noida Call Girls Number 9999965857 Vip Call Girls Lady Of Your Dream Ready To Meet Nearby At Home/Hotel
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
一比一原版(macewan学位证书)加拿大麦科文大学毕业证如何办理
原版一模一样【微信：741003700 】【(macewan学位证书)加拿大麦科文大学毕业证成绩单】【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
办理(macewan学位证书)加拿大麦科文大学毕业证【微信：741003700 】外观非常简单，由纸质材料制成，上面印有校徽、校名、毕业生姓名、专业等信息。
办理(macewan学位证书)加拿大麦科文大学毕业证【微信：741003700 】格式相对统一，各专业都有相应的模板。通常包括以下部分：
校徽：象征着学校的荣誉和传承。
校名:学校英文全称
授予学位：本部分将注明获得的具体学位名称。
毕业生姓名：这是最重要的信息之一，标志着该证书是由特定人员获得的。
颁发日期：这是毕业正式生效的时间，也代表着毕业生学业的结束。
其他信息：根据不同的专业和学位，可能会有一些特定的信息或章节。
办理(macewan学位证书)加拿大麦科文大学毕业证【微信：741003700 】价值很高，需要妥善保管。一般来说，应放置在安全、干燥、防潮的地方，避免长时间暴露在阳光下。如需使用，最好使用复印件而不是原件，以免丢失。
综上所述，办理(macewan学位证书)加拿大麦科文大学毕业证【微信：741003700 】是证明身份和学历的高价值文件。外观简单庄重，格式统一，包括重要的个人信息和发布日期。对持有人来说，妥善保管是非常重要的。
Anti-Universe And Emergent Gravity and the Dark Universe
Recent theoretical progress indicates that spacetime and gravity emerge together from the entanglement structure of an underlying microscopic theory. These ideas are best understood in Anti-de Sitter space, where they rely on the area law for entanglement entropy. The extension to de Sitter space requires taking into account the entropy and temperature associated with the cosmological horizon. Using insights from string theory, black hole physics and quantum information theory we argue that the positive dark energy leads to a thermal volume law contribution to the entropy that overtakes the area law precisely at the cosmological horizon. Due to the competition between area and volume law entanglement the microscopic de Sitter states do not thermalise at sub-Hubble scales: they exhibit memory effects in the form of an entropy displacement caused by matter. The emergent laws of gravity contain an additional ‘dark’ gravitational force describing the ‘elastic’ response due to the entropy displacement. We derive an estimate of the strength of this extra force in terms of the baryonic mass, Newton’s constant and the Hubble acceleration scale a0 = cH0, and provide evidence for the fact that this additional ‘dark gravity force’ explains the observed phenomena in galaxies and clusters currently attributed to dark matter.
Mapping the Growth of Supermassive Black Holes as a Function of Galaxy Stella...
The growth of supermassive black holes is strongly linked to their galaxies. It has been shown that the population
mean black hole accretion rate (BHAR) primarily correlates with the galaxy stellar mass (Må) and redshift for the
general galaxy population. This work aims to provide the best measurements of BHAR as a function of Må and
redshift over ranges of 109.5 < Må < 1012 Me and z < 4. We compile an unprecedentedly large sample with 8000
active galactic nuclei (AGNs) and 1.3 million normal galaxies from nine high-quality survey fields following a
wedding cake design. We further develop a semiparametric Bayesian method that can reasonably estimate BHAR
and the corresponding uncertainties, even for sparsely populated regions in the parameter space. BHAR is
constrained by X-ray surveys sampling the AGN accretion power and UV-to-infrared multiwavelength surveys
sampling the galaxy population. Our results can independently predict the X-ray luminosity function (XLF) from
the galaxy stellar mass function (SMF), and the prediction is consistent with the observed XLF. We also try adding
external constraints from the observed SMF and XLF. We further measure BHAR for star-forming and quiescent
galaxies and show that star-forming BHAR is generally larger than or at least comparable to the quiescent BHAR.
Unified Astronomy Thesaurus concepts: Supermassive black holes (1663); X-ray active galactic nuclei (2035);
Galaxies (573)
Physics Investigatory Project on transformers. Class 12th
Physics investigatory project on transformers with required details for 12thes. with index, theory, types of transformers (with relevant images), procedure, sources of error, aim n apparatus along with bibliography🗃️📜. Please try to add your own imagination rather than just copy paste... Hope you all guys friends n juniors' like it. peace out✌🏻✌🏻
Recently uploaded (20)
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Evaluation and Identification of J'BaFofi the Giant Spider of Congo and Moke...
Evaluation and Identification of J'BaFofi the Giant Spider of Congo and Moke...
SDSS1335+0728: The awakening of a ∼ 106M⊙ black hole⋆
SDSS1335+0728: The awakening of a ∼ 106M⊙ black hole⋆
BIRDS DIVERSITY OF SOOTEA BISWANATH ASSAM.ppt.pptx
BIRDS DIVERSITY OF SOOTEA BISWANATH ASSAM.ppt.pptx
acanthocytes_causes_etiology_clinical sognificance-future.pptx
acanthocytes_causes_etiology_clinical sognificance-future.pptx
Embracing Deep Variability For Reproducibility and Replicability
Embracing Deep Variability For Reproducibility and Replicability
Noida Call Girls Number 9999965857 Vip Call Girls Lady Of Your Dream Ready To...
Noida Call Girls Number 9999965857 Vip Call Girls Lady Of Your Dream Ready To...
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
Anti-Universe And Emergent Gravity and the Dark Universe
Anti-Universe And Emergent Gravity and the Dark Universe
Mapping the Growth of Supermassive Black Holes as a Function of Galaxy Stella...
Mapping the Growth of Supermassive Black Holes as a Function of Galaxy Stella...
Physics Investigatory Project on transformers. Class 12th
Physics Investigatory Project on transformers. Class 12th
enzyme kinetics.pptx
- 1. FACTORS AFFECTING ENZYME ACTIVITY Presented By Dr. Anuradha
- 2. FACTORS AFFECTING ENZYME ACTIVITY The contact between the enzyme and substrate is the most essential pre-requisite for enzyme activity. 1. Concentration of enzyme: As the concentration of the enzyme is increased, the velocity of the reaction proportionately increases .
- 3. 2. Concentration of substrate: Increase in the substrate concentration gradually increases the velocity of enzyme reaction within the limited range. A rectangular hyperbola is obtained when velocity is plotted against the substrate concentration.
- 4. Order of reaction : When velocity of reaction ~ substrate concentration, rate of the reaction is first order. Enzyme kinetics and Km value : The enzyme (E) and substrate (S) combine with each other to form an unstable enzyme-substrate complex (ES) for the formation of product (P).
- 5. k1, k2 and k3=velocity constants. Km the Michaelis-Menten constant The following equation is obtained after suitable algebraic manipulation. where V= Measured velocity Vmax = Maximum velocity S = Substrate concentration Km = Michaelis-Menton constant.
- 6. Let us assume that the measured velocity (v) = ½ Vmax. Then the equation may be substituted as follows: K stands for a constant and m stands for Michaelis (in Km). Km is defined as the substrate concentration (expressed in moles/l) to produce half-maximum velocity in an enzyme catalysed reaction.
- 7. • Half of the enzyme molecules (i.e. 50%) are bound with the substrate molecules when substrate concentration = Km value. • Representative for measuring the strength of ES complex. • A low Km value indicates a strong affinity between enzyme and substrate. • High Km value reflects a weak affinity between them. • For majority of enzymes, the Km values are in the range of 10-5 to 10-2 moles. • Km is not dependent on the concentration of enzyme.
- 8. Lineweaver-Burk double reciprocal plot • For the determination of Km value the substrate saturation curve is not very accurate since V max is approached. • By taking the reciprocals of the equation (1), a straight line graphic representation is obtained.
- 9. • The above equation is similar to y = ax + b. • A plot of velocity [1/V] vs the reciprocal of the substrate concentration {1/[S]} gives a straight line. • The slope is km/Vmax and whose y intercept is 1/Vmax. • Lineweaver-Burk plot is much easier to calculate the Km. • From the intercept on x-axis which is -(l/Km). • Double reciprocal plot is useful in understanding the effect of various inhibitions.
- 11. 3. Effect of temperature • Velocity of an enzyme reaction increases with increase in temperature up to a maximum and then declines. • A bell-shaped curve is usually observed.
- 12. • Increase in temperature---higher activation energy • More molecular (enzyme and substrate) collision and interaction for the reaction to proceed faster. • The optimum temperature for most of the enzymes is between 40°C-45°C. • Few enzymes (e.g. venom phosphokinases, muscle adenylate kinase) are active even at 100°C.
- 13. • Some plant enzymes like urease have optimum activity around 60°C. • Exposed to a temperature above 50°C, denaturation leading to derangement in the native structure. • Majority of the enzymes become inactive at higher temperature (above 70°C).
- 14. 4. Effect of pH • Increase in the hydrogen ion concentration (pH) a bell-shaped curve is normally obtained. • Each enzyme has an optimum pH at which the velocity is maximum. • Below optimum pH, the enzyme activity is much lower.
- 15. • Extreme pH, the enzyme becomes totally inactive. • Enzymes of higher organisms show optimum activity around neutral pH (6-8). • Exceptions like pepsin (1-2), acid phosphatase (4- 5) and alkaline phosphatase (10-11). • Enzymes from fungi and plants are most active in acidic pH (4-6).
- 16. 5.Effect of product concentration • The accumulation of reaction products generally decreases the enzyme velocity. • Certain enzymes form a loose complex and inhibit the enzyme activity. • This type of inhibition is generally prevented by a quick removal of products formed.
- 17. 6. Effect of activators • Some of the enzymes require certain inorganic metallic cations like Mg2+, Mn2+, Zn2+, Ca2+, Cu2+, Na+, K+ etc for their optimum activity • Rarely, anions are also needed e.g. chloride ion. • Metals as activators of enzyme velocity by combining with the substrate • Formation of ES-metal complex direct participation in the reaction conformational change.
- 18. Metal-activated enzymes: Metal is not tightly held by the enzyme and can be exchanged easily with other ions e.g. ATPase (Mg2+ and Ca2+) Enolase (Mg2+) Metalloenzymes: Enzymes hold the metals tightly which are not readily exchanged. e.g. Phenol oxidase (copper) Pyruvate oxidase (manganese)
- 19. 7. Effect of time • Under ideal and optimal conditions (pH, temperature etc.), the time required for an enzyme reaction is less. • Variations in the time of the reaction are generally related to the alterations in pH and temperature