The document summarizes a lab on the respiratory system and spirometry. It describes the parts of the respiratory system including the conduction zone for ventilation and respiratory zone for gas exchange. It examines histology samples of different parts of the respiratory system and how the structure relates to function. The document then discusses spirometry and how it is used to measure lung volumes and capacities like tidal volume, vital capacity, and residual volume. Procedures for calibrating and performing spirometry tests on the Biopac system are provided along with an example spirogram.

1. The Respiratory System & Spirometry Lab 6

2. Lab 6 Activities Identify parts of the respiratory system Respiratory tree, thoracic organs, and larynx models Fresh lung (if available) Examine histology of the: Trachea Lung Examine the operation of the lung model Biopac L012-Lung-1: Spirometry Measure lung volumes and capacities

3. Respiratory System Structure Conduction zone: pathway for pulmonary ventilation Respiratory zone: membrane for gas exchange  external respiration Clinically, two parts: Upper respiratory tract Lower respiratory tract

4. Respiratory System Structure Histology reflects the different functions of the different parts of the system Position in respiratory pathway determines cell type non-keratinized stratified squamous pseudostratified ciliated columnar cuboidal ciliated simple squamous / Type I Alveolar cells

6. Pseudostratified Columnar Epithelium Lamina Propria Hyaline Cartilage mucosa Submucosa

7. seudostratified epithelium = lamina propria ucous glands mooth muscle artilage

8. Gross Section Through Lung

9. Smoker’s Lungs Non-smoker 

10. Small Bronchiole

11. Lung Tissue slide Respiratory Bronchiole Alveolar Duct Alveoli Alveolar Sac

12. Capillaries in Alveolar Wall

13. Spirometry

14. SPIROMETRY – RESPIRATORY VOLUMES AND CAPACITIES Total Lung Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) + Residual Volume (RV) Vital Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) Inspiratory Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) Functional Residual Capacity = Expiratory Reserve Volume (ERV) + Residual Volume (RV)

15. Spirometry – Calibration Insert the calibration syringe/filter assembly into the side of the airflow transducer labeled " Inlet ." Inlet Wait for 8 seconds of no air flow Inlet

16. Experimental Treatments 4 normal breaths Inhale as deeply as possible Exhale to normal depth 3 normal breaths Exhale as much as possible 3 normal breaths Inhale as much as possible Exhale as fast and completely as possible 4 normal breaths

17. BioPac Spirogram Example Data 3 regular breaths 3 regular breaths 3 regular breaths 1 strongest inhale 1 regular exhale 1 strongest inhale 1 strongest exhale 1 strongest exhale This part is in addition to what Biopac Lesson 12-Lung 1 prompts you to do! Do Data Analysis on the lower red Volume curve.

18. Summary of Class Data Link to Table

19. The End Lab 6

20. Biopac Spirometry Residual volume

21. Spirometry