Jauhar ali. vol 5. screening for abiotic and biotic stress tolerances

1. Basic Molecular Breeding 2:Basic Molecular Breeding 2:Basic Molecular Breeding 2:Basic Molecular Breeding 2:
S3-Y2
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
Jauhar Ali
Plant Breeder, Senior Scientist
IRRI-GSR Project Leader & Regional Coordinator (Asia)
PBGB, IRRI (J.Ali@irri.org)
GSRIR1-8-S6-S3

2. Why there is a necessity for
breeding for resilience?
• Food Security 2008–looming threat-higher yields under
ever reducing resources
• Stable sustainable yields using lesser inputs(SSNM)-
farmer practice-rainfed complex abiotic stresses-stressed
irrigated condition- Lesser inputs-complex conditions
• Diseases & Insect pest threats-high input environments-• Diseases & Insect pest threats-high input environments-
Broad spectrum-durable R
• Caring for environment-pollution of water systems-
chemical residues-Reduction of C’s
• Changing climatic situations-vulnerability-heat-flooding-
drought- Multiple Stress Tolerance

3. RACE AGAINST TIME

4. Drought stress frequency map in
Fess et al (2011) Crop Breeding for Low Input Agriculture:
A Sustainable Response to Feed a Growing World
Population. Sustainability 2011, 3(10), 1742-1772
Drought stress frequency map in
rainfed rice areas based on rainfall
anomalies from 1960-2002.
Flood frequency map in rice growing areas based on
100 years of UNEP flood risk data.Andy et al (2012) IRRI GSR Report

5. Rice Ecosystems Breeding for Drought prone environments: Fischer et al 2003

6. TargetTraits
Yield & Grain
Abiotic
Drought
Salinity
Submergence
Anaerobic
germination
Low input
environments
Early maturity
can be useful trait to
escape both the
stresses.
Grain quality
matters a lot at the
end
Most donors will be
able to throw
transgressive
TargetTraits
Yield & Grain
Quality
Biotic
BLB
BLAST
TUNGRO/RSV
BLS
Sheath blight
transgressive
segregants
Identify extreme
transgressive
segregants & their
donors
Place the tolerant
checks, DP, RP for
convenient selection

7. ABIOTIC STRESSES
• Drought
• Submergence
• Salinity-alkalinity
• Anaerobic Germination• Anaerobic Germination
• Low soil fertility soils-N, P, K
• Problem soils-Al, Fe toxicity, acid sulphate,
peat soils

8. Salt Stresses and Associated Complexities
S
A
L
T
S
Acid
SO4
S
A
L
I
N
E
(P, Zn)
(P, Zn)
Fe, Al
tox
Fe, H2S
tox
Al, Organic
R
A
I
N
F
E
D
Sub-
merged
Deep-
water
Drought
G
r
a
I
n
S
T
R
E
S
S
E
S
Peat
E
ALKALINE
INLAND
SALINE
(P, Zn)
(P, Zn)
(Fe)
Al, Organic
Acids tox
(P & Zn)
D Drought
Irrigated
Q
u
a
l
i
t
y
(Source: Glenn B. Gregorio)

9. Breeding for Resilience-“GSR”
Rice c
ultivars that produce higher & more stable yields with lesser inputs
(water, fertilizers &pesticides)
High yielding GSR cultivars with “Green” traits:
Resistances/ tolerances to:Resistances/ tolerances to:
Abiotic stresses: Drought, salinity, alkalinity, iron toxicity, etc.
Diseases: Blast, BLB, sheath blight, BLS viruses & false smut
Insects: BPH, GLH, stem borer etc
Grain quality Mostly in elite RP background- later in RP-NARES
High resource-use efficiency: Water and nutrients (N P K)
Less inputs, more production & environment sustainabilityLess inputs, more production & environment sustainability

10. RP (3) x donors(205) F1s x RP BC1F1s x RP
~25 BC2F1s/donor x RP
x
Bulk BC2F2 populations
BC3F1s x RP
1, 2, 3, 4, 5, 6, ……
BC3F2 populations
Self and bulk
harvest
Selection for target traits
and backcrossing
BC4F1s
BC4F2s
x
x
Self and bulk
harvest
1, 2, 3, 4, 5, 6, ……
Development of GSR materials by designed QTL pyramiding (DQP)
strategy for select target component traits for a given ecosystem
Alietal(2006)FCR97:66-76
Confirmation of the selected traits by replicated phenotyping
and genotyping of ILs for gene/QTL identification
Crosses made between sister ILs
having unlinked desirable genes/
QTLs for target ecosystem
DQP &MAS for pyramiding desirable
genes/QTLs and against undesirable donor
segments for target ecosystem
Development of GSR materials with improved target traits for wide scale
testing in different ecosystems and its release.
NILs for individual genes/QTLs for functional genomic studies
Screening for target traits such as tolerances to
drought, salinity, submergence, anaerobic
germ., P & Zn def., BPH, etc.
Alietal(2006)FCR97:66

11. Development of ILS for different abiotic and
biotic stress tolerances at IRRI
Ali et al (2006) FCR 97:66-76

12. Hidden diversity for abiotic and biotic tolerance inHidden diversity for abiotic and biotic tolerance in
the primary gene pool of ricethe primary gene pool of rice
• Tremendous amounts of hidden diversity-BC progeny-transgressive -target traits-
regardless of donor performance-severe stress screening
• Common to identify in BC progeny-extreme phenotypes (tolerances)
• Selection efficiency –highly dependent upon background
• Selection efficiency-affected by level of stress applied
• Selection efficiency for different target traits vary in BC generations.
• More distantly related donors, particularly landraces, tend to give more transgressive
segregations for complex phenotypes in the BC progenies.
• Wide presence and random distribution of stress tolerance genes in primary gene
pool of rice –good news for rice breeders
Yu et al (2003) TAG 108:131-140; Ali et al (2006) FCR 97:66-76

13. RM380.0
RM2516.1
RM7234.8
RM4440.4
RM33943.8
8
RM2850.0
RM21911.8
RM10534.7
9
RM20A0.0
RM1919.1
RM24736.0
12
Chromosomal location of QTLs for BPH
in IR64/Binam BC2F4 population
(4.15) (11.64)
(13.5)
RM4259.1
RM25668.7
RM25786.8
RM24294.2
RM20196.7
RM205111.1
RM26073.7
RM17123.9
QBph
(4.15) (11.64)
(7.43)
IL 64 (IR64 x Binam)
IL 80 (IR64 x Binam)
BPHscreening
BPH
36.7%

14. Rice hybrids for rainfed lowlands: AnaerobicRice hybrids for rainfed lowlands: Anaerobic
germination a key traitgermination a key trait
• 19% area; 1-1.5t/ha
• Poor seedling establishment
• 11 ILs(BC4F2) with complete germination
4.90
28.7
33.7
37.0
RM60
RM231
RM7
RM232
RM251
0.0 QAna1(10.0)
Chr3
IR64/Binam BC2F4 popln.
• 11 ILs(BC4F2) with complete germination
in NPT background
• Two of them were promising 7235,
7237(TKM-9)
• Efforts are underway to pyramid it with
submergence tolerance.
RM130.0
RM2899.0
RM16337.7
RM16442.0
RM16148.1
RM17862.1
(11.0)
(27.8)
QAna2
QAna3
Chr5
54.3
72.8
85.10
RM130124.4
160.0
RM282
RM16
RM203
RM85

15. FAVOURABLE DONORS (VARY ACCORDING TO RP)FAVOURABLE DONORS (VARY ACCORDING TO RP)S.No.S.No.
OM1706,OM1723,OM1706,OM1723,FR13AFR13A,NAN29,NAN29--2,BABOAMI, KHAZAR2,BABOAMI, KHAZARSTST
TKM9,TKM9,HEIHEI--HEHE--AIAI--HUI(HHAH),JIANGXIHUI(HHAH),JIANGXI--SISI--MIAO(JSM), KHAZAR, MADHUKAR,MIAO(JSM), KHAZAR, MADHUKAR,
SHWESHWE--THWETHWE--YINYIN--HYE (STYH), BASMATI385, IKSAN438, YUHYE (STYH), BASMATI385, IKSAN438, YU--QIUQIU--GU, TETEP,GU, TETEP,
NIPPONBARE, CO43, RASI, YUNHUI, BG304,BR24,NIPPONBARE, CO43, RASI, YUNHUI, BG304,BR24, FR13AFR13A GAYABYEOGAYABYEO
ZDTZDT
Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29--2,2,
AGAG
Donors that gave better results with varying
recurrent parental backgrounds
Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29Y134,TKM9,KHAZAR,GAYABYEO,STYH,NAN29--2,2,
BABOAMI,JSM,BABOAMI,JSM,FR13AFR13A,OM1706,OM1706AGAG
CISEDANE,CISEDANE,FR13AFR13A,IR50,NAN29,IR50,NAN29--2,OM1706,STYH,TAROM MOLAEI,2,OM1706,STYH,TAROM MOLAEI,TKM9TKM9,Y134,Y134SUBTSUBT
NAN29NAN29--2,GAYABYEO2,GAYABYEOLTGLTG
JSM,BABOAMI,JSM,BABOAMI,TKM9TKM9,BG300,C418,LEMONT,MADHUKAR,MR167,OM1706,STYH,,BG300,C418,LEMONT,MADHUKAR,MR167,OM1706,STYH,
Y134Y134BPHBPH
BABOAMI, GAYABYEO, SHWEBABOAMI, GAYABYEO, SHWE--THWETHWE--YINYIN--HYE (STYH), NAN29HYE (STYH), NAN29--2, FR13A,2, FR13A,
OM1706, KHAZAR, JIANGXIOM1706, KHAZAR, JIANGXI--SISI--MIAOMIAO
MULTIMULTI--
TRAITSTRAITS
Ali et al (2006) FCR 97:66-76

16. ExperimentsetI
IR64 x BR24
F1 x IR64
BC2F2
IR64 x Binam IR64 x STYH
F1 x IR64
BC2F2
IR64 x OM1723
F1 x IR64
BC2F2
F1 x IR64
BC2F2
13 BC2F2 populations screened under two types of severe drought, resulting in 221 survived
DT BC2F3 introgression lines (ILs), which were genotyped with SSR markers
IR64 x Type3
F1 x IR64
BC2F2
IR64 x HAN
F1 x IR64
BC2F2
IR64 x Zihui100
F1 x IR64
BC2F2
ExperimentsetII
IL1 x IL2
F1
X
IL3 x IL4
F1
X
IL7 x IL15
F1
X
9 1st round pyramiding
F2 populations from
crosses between 15 ILs
Designed QTL pyramiding experimentsExperimentsetII
Screened under severe drought at the reproductive stage, resulting in 455 survived
DT F2 plants, which were progeny tested and genotyped with SSR markers
F2
X
F2
X
F2
X
crosses between 15 ILs
ExperimentsetIII
Screened under severe drought at the reproductive stage and 667 survived
DT F3 lines were progeny tested and genotyped with SSR markers
(PL1 , PL2, PL3) x (PL4, PL5, PL6, PL7, PL8)
F1s
F2s
X
14 2nd round pyramiding F2
populations from crosses
between 8 1st round PLs

17. IL1 F2 IL2
The 1st pyramiding F2 populations and their parents
screened under severe lowland drought at the
reproductive stage (2002-03 DS)

18. F2 population
Field screening of the 2nd round
pyramiding F2 populations under severe
lowland stress (2005-2006 dry season)
Parental 1st round PLs and IR64
Parental lines

19. Putative genetic networks identified in 455 DT PLs derived
from 9 crosses between DT IR64 ILs
Drought
AG2-1 (5)
0.994
RM575
(1.4)
0.745
RM342
(8.5)
0.673
AG2-2 (6)
0.891
RM347
(3.8)
0.691
RM469
(6.1)
0.818
RM215
(9.8)
0.527
RM561
(2.6)
0.618
RM544
(8.2)
0.727
RM309
(12.5)
0.927
RM202
(11.3)
0.745
RM463
(12.5)
0.745
RM179
(12.3)
0.727
B:Drought
AG1-1 (7)
1.00
AG1-3
(13)
0.748
AG1-2 (7)
0.979
AG1-5
(5)
0.726
RM418
(7.3)
0.717
AG1-4
(4)
0.688
RM109
(2.1)
0.617
RM179
(12.3)
0.607
A:
DroughtD:
AG
AG7-1 (18)
1.00
Drought
G:
I: Drought
AL9-1 (3)
1.000
RM152
(8.1)
0.930
AG9-5(3)
0.553
AG9-2(2)
0.915
RM211
(2.2)
0.800
RM446
(1.6)
0.830
RM350
(8.4)
0.800
AG9-4 (5)
0.500
AG9-3(24)
0.870
RM215
(9.7)
0.870
RM554
(3.7)
0.700
Drought
RM543
(1.1)
E:
Drought
AG3-1 (4)
1.00
AG3-2
(4)
0.855
RM302
(1.10)
0.782
AG3-3
(3)
0.736
RM172
(7.7)
0.727
C:
AG4-1
(6)
RM271
(10.4)
RM23
(1.5)
AG4-2
(4)
AG4-3
(4)
AG4-4
(3)
RM544
(8.3)
RM179
(12.3)
RM215
(9.8)
RM220
(1.2)
RM272
(1.3)
RM441
(11.2)
RM36
(3.3)
AG7-5
(2)
AG7-2
(2)
AG7-3
(16)
AG7-7
(2)
AG7-4
(7)
RM275
(6.6)
RM110
(2.1)
RM224
(11.7)
RM294B
(1.6)
RM435
(6.1)
RM13
(5.2)
RM5
(1.7)
RM245
(9.8)
RM30
(6.8)
RM18
(7.6)
RM465A
(2.5)
RM469
(6.1)
RM286
(11.1)
RM289
(5.3)
RM44
(8.3)
RM516
(5.3)
RM85
(3.12)
AG8-1 (26)
1.00
RM448
(3.10)
RM331
(8.4)
RM481
(7.1)
RM535
(2.12)
DroughtH:
RM32
(8.3)
AG8-2
(2)
RM30
(6.7)
AG8-3
(3)
RM562
(1.6)
RM547
(8.3)
RM275
(6.5)
RM143
(3.12)
RG8-6
(2)
RM197
(6.1)
RM5
(1.7)
RM307
(2.1)
RM449
(1.6)
RM14
(1.13)
RM169
(5.3)
AG8-4
(3)
RM246
(1.8)
AG8-5
(2)
RM589
(6.1)
RM317
(4.6)
RM258
(10.4)
RM154
(2.1)
RM245
(9.8)
RM335
(3.12)
RM446
(1.6)
RM211
(2.2)
Drought
AG6-1 (8)
1.000
AG6-3
(12)
0.894
AG6-2 (5)
0.967
RM44
(8.3)
0.633
RM235
(12.6)
0.667
AG6-4
(2)
0.772
RM51
(7.1)
0.833
RM20
12.1
0.567
F:
(1.1)
1.000
AG5-4 (2)
0.767
RM53
(2.3)
0.833
AG5-1 (12)
0.711
AG5-2 (9)
0.809
RM401
(4.1)
0.733
RM433
(8.7)
0.867
RM298
(7.1)
0.767
RM17
(12.7)
0.500
RM270
(12.6)
0.567
RM222
(10.1)
0.567
RM424
(2.5)
0.667
AG5-3(2)
0.525
RM244
(10.1)
0.583
RM248
(7.7)
0.500
RM101
(12.4)
0.766
Li et al 2011 unpubl.

20. Ch.2Ch.1 Ch.3 Ch.4 Ch.5 Ch.6
RM109
RM485
RM154
RM211
RM236
RM279
RM423
RM8
RM53
RM233A
RM174
RM145
RM71
RM327
RM521
RM300
RM324
RM424
RM262
RM341
RM475
RM106
RM263
RM526
RM221
RM525
RM318
RM450
RM497
RM6
RM240
RM530
RM112
RM250
RM166
RM197
RM213
RM48
RM207
RM266
RM138
RM307
RM401
RM537
RM335
RM518
RM261
RM471
RM142
RM273
RM252
RM241
RM470
RM303
RM317
RM348
RM349
RM131
RM280
RM567
RM559
RM122
RM153
RM413
RM13
RM267
RM437
RM289
RM509
RM598
RM163
RM164
RM291
RM161
RM188
RM421
RM178
RM26
RM274
RM87
RM480
RM538
RM334
RM399
RM169
RM204
RM587
RM588
RM589
RM510
RM204
RM585
RM111
RM225
RM314
RM253
RM50
RM549
RM539
RM136
RM527
RM3
RM343
RM528
RM30
RM340
RM400
RM439
RM103
RM141
RM176
RM494
RM557
RM584
RM60
RM81B
RM22
RM523
RM569
RM231
RM175
RM545
RM517
OSR13
RM7
RM232
RM251
RM282
RM338
RM156
RM411
RM487
RM16
RM504
RM203
RM186
RM55
RM168
RM416
RM520
RM293
RM114
RM130
RM565
RM14
OSR23
RM431
RM472
RM297
RM265
RM319
RM315
RM128
RM212
RM403
RM473A
RM246
RM237
RM306
RM5
RM9
RM594
RM323
RM84
RM428
RM220
RM86
RM522
RM283
RM1
RM272
RM575
RM490
RM576
RM259
RM583
RM243
RM600
RM572
RM580
RM581
RM23
RM129
RM446
RM329
RM562
Bin1.1
Bin1.2
Bin1.3
Bin1.4
Bin1.5
Bin1.6
Bin1.7
Bin1.8
Bin1.9
Bin1.10
Bin1.11
Bin1.12
Bin1.13
Bin2.1
Bin2.2
Bin2.3
Bin2.4
Bin2.5
Bin2.6
Bin2.7
Bin2.8
Bin2.9
Bin2.10
Bin2.11
Bin2.12
Bin3.1
Bin3.2
Bin3.3
Bin3.4
Bin3.5
Bin3.6
Bin3.7
Bin3.8
Bin3.9
Bin3.10
Bin3.11
Bin4.1
Bin4.2
Bin4.3
Bin4.4
Bin4.5
Bin4.6
Bin4.7
Bin4.8
Bin6.1
Bin6.2
Bin6.3
Bin6.4
Bin6.5
Bin6.6
Bin6.7
Bin6.8
Bin6.9
Bin5.1
Bin5.2
Bin5.3
Bin5.4
Bin5.5
Bin5.6
Bin5.7
RM245
Binam segments
BR24 segments
STYH segments
OM1723 segments
RM462
RM555
RM516
RM190
RM454
RM162
RM561
RM540
RM469
RM302
RM488
RM347
RM535
RM233B
RM19029
RM19778
RM499
RM10287
RM14963
RM11570
RM551
Cross III-2
Cross III-1
FGUs identified in cross II-1
FGUs identified in cross II-2
FGUs identified in cross II-3
RM565
RM514
RM570
RM227
RM85
Ch.8 Ch.9 Ch.10 Ch.11 Ch.12Ch.7
RM474
RM222
RM216
RM239
RM311
RM467
RM184
RM271
RM269
RM258
RM171
RM304
RM228
RM147
RM333
RM496
RM436
RM51
RM481
RM125
RM180
RM501
OSR22
RM214
RM418
RM432
RM11
RM346
RM182
RM336
RM10
RM351
RM455
RM505
RM234
RM18
RM172
RM248
RM408
RM506
RM407
OSR30
RM544
RM25
RM407
RM44
RM72
RM137
RM331
RM339
RM342A
RM515
RM284
RM210
RM556
RM256
RM149
RM230
RM264
RM281
RM296
RM285
RM316
RM444
RM219
RM524
RM105
RM321
RM409
RM460
RM566
RM434
RM257
RM108
RM242
RM278
RM201
RM107
OSR28
RM189
RM215
RM205
RM286
RM332
RM167
RM120
RM479
RM181
RM202
RM536
RM260
RM287
RM209
RM229
RM457
RM187
RM21
RM206
RM254
RM224
RM144
RM20A
RM4A
RM19
RM247
RM512
RM179
RM101
RM277
RM511
RM519
RM313
RM309
RM463
RM235
RM270
RM17
RM4B
Bin3.12
Bin10.7
Bin10.1
Bin10.2
Bin10.3
Bin10.4
Bin10.5
Bin10.6
Bin7.1
Bin7.3
Bin7.5
Bin7.7
Bin7.2
Bin7.4
Bin7.6
Bin8.8
Bin8.1
Bin8.2
Bin8.3
Bin8.4
Bin8.5
Bin8.6
Bin8.7 Bin9.8
Bin9.1
Bin9.2
Bin9.3
Bin9.4
Bin9.5
Bin9.6
Bin9.7
Bin11.7
Bin11.1
Bin11.3
Bin11.4
Bin11.5
Bin11.6
Bin12.5
Bin12.1
Bin12.2
Bin12.3
Bin12.4
Bin12.6
Bin12.7
RM547
RM447
Bin11.2
Binam segments
Genomic correspondences between FGUs identified in 150 ILs of 8 BC2 populations,
200 PLs of 3 1st round pyramiding crosses and 4 2nd round pyramiding crosses.
RM223
RM126
RM25022
RM23818
RM245
RM25181
RM473E
RM26063
Cross III-2
Cross III-1
Cross III-3
Cross III-4
Li et al 2011 (unpubl)

21. Meanyieldunderthe
irrigatedcontrol(t/ha)
1.5
2.0
2.5
3.0
Type III (N=19)
C: 5.06±±±±0.47
VS: 1.98±±±±0.47
RS: 1.94±±±±0.52
Type I (N=17)
C: 5.76±±±±0.53
VS: 2.07±±±±0.55
RS: 1.79±±±±0.47
Type II (N=5)
C: 5.71±0.42
VS: 1.36±0.38
RS: 2.20±0.45
Type IV (N=7)
C: 4.66±0.48
VS: 1.34±0.41
RS: 1.86±0.51
IR64 (CK)
C: 4.68±0.23
VS: 1.49±0.14
RS: 0.52±0.38
4.0
4.5
5.0
5.5
6.0
6.5
The mean yield performances (t/ha) of 48 2nd round PLs (4 types) as
compared to IR64 (CK), under the irrigated control (C), drought stresses
at the vegetative (VS) and reproductive stages (RS) in the 2007 and 2008
dry-season. Guan et al. 2010 JXB
3.0
0.5
1.0
1.5 2.0 2.5
0.5
1.0
1.5
3.5
3.0
0.0

22. 6 sqm plot
100 hills yield
from the
center of the
plot
3 replication
1 month
stressed
irrigation
No irrigation
till harvest in
DS2010 IRRI

23. GSR Drought tolerant pyramided lines in IR64 background
Under zero input conditions at IRRI DS2010
DT Check variety
IR74371-70-1-1
GSR-IR83142-B-19-B

24. ILOILO GSR SALINITY TRIALS
2008:Salinity pyramided lines at ILOILO
Replicated yield trials of stable 10 salinity tolerant pyramiding
lines under Iloilo hot spot (18.8dSm-1 ; pH 6.3) for salinity
conditions showing immense potential.
Evaluation of 62 GSR materials in saline field condition in Ajuy, Iloilo 2reps
Highly saline (EC16.2dS/m at transplanting, EC18 for 3 weeks and EC 20 after 4
weeks of transplanting)
Trial conducted by Dr Glenn, PBGB

26. GSR entryGSR entry No ofNo of
paniclespanicles
PlantPlant
heightheight
(cm)(cm)
MaturityMaturity
(days)(days)
YieldYield
(kg/ha)(kg/ha)
%%
increaseincrease
overover
FL478FL478
SESSES
scorescore
4WAT4WAT
SESSES
scorescore
MaturityMaturity
IR83140IR83140--BB--1111--BB 1616 8484 116116 11401140 103.6103.6 44 55
Promising GSR Drought + Salinity tolerant materials tested under Iloilo during WS2010
1616 8484 116116 11401140 103.6103.6 44 55
IR83140IR83140--BB--2828--BB 1313 8686 114114 876876 56.456.4 44 55
IR83140IR83140--BB--3232--BB 1515 8585 114114 657657 17.317.3 44 55
FL478FL478 1111 7070 111111 560560 0.00.0 55 --
NSIC 222NSIC 222 1919 8383 112112 147147 --73.873.8 44 --
First two nominated for NCT Philippines WS2011

27. DT PDLs AMMI-Biplot: 6 Locations -2011DS
BRAC-Gaz, VAAS-Gia, VAAS-Duo, ICRR-Jak, ICRR-Teg, & IRRI-Los Banos
Entry
No.
GSR Lines
Mean
(t/ha)
LSD
Group
15 IR 83142-B-57-B 5.46 a
9 IR 83141-B-17-B 5.17 b
19 IR 83142-B-7-B-B 5.13 bc
18 IR 83142-B-79-B 5.12 bc
11 IR 83142-B-19-B 5.06 bcd
5 IR 83140-B-11-B 5.05 bcde
10 IR 83141-B-18-B 5.02 bcdef
0.00.5
PC2
1
2
4
68
10
11
12
13
16
17
1819
10amBrGa
10dsIcJa
10dsIRig
10suVaDu10suVaGi
1
2
PC %
60.9
24.7
IR 83142-B-19-B
2nd Best Check
methylationJXBDOI:10.1093/jxb/erq391
17% adv.
6 IR 83140-B-28-B 4.94 bcdefg
13 IR 83142-B-21-B 4.86 cdefg
12 IR 83142-B-20-B 4.79 defg
14 IR 83142-B-49-B 4.78 efg
16 IR 83142-B-60-B 4.75 fg
20 IR 83142-B-8-B-B 4.74 g
7 IR 83140-B-32-B 4.74 g
3 Best Check 4.67 g
8 IR 83140-B-36-B 4.32 h
1 2nd Best Check 4.29 h
17 IR 83142-B-61-B 4.27 h
4 IR 74371-70-1-1 3.57 i
2 Apo 3.53 i
-0.5 0.0 0.5 1.0
-1.0-0.5
PC 1
PC2
3
5
7
9
11
14 15
20
10dsIcTe
10suVaDuIR 83142-B-19-B
Best
Check
IR 83140-B-11-B
Environments
Mean
(t/ha)
LSD
Group
IRRI-Los
Banos
6.55 a
VAAS-Gia 6.53 a
VAAS-Duo 6.06 b
BRAC-Gaz 4.29 c
ICRR-Jak 3.18 d
ICRR-Teg 2.08 e
IR 83142-B-57-B
Why such yield advantages?
IL breeding + Designed QTL Pyramiding
Selection for grain yield, higher spikelet
fertility, deeper and thicker roots esp.
under reproductive stage DT stress
Wangetal2010DroughtinducedsitespecificDNAmethylation

28. IR83140-B-11-B
PVS Purvakarta
2.5ha trial area
Indonesia 8.2011
High protein 11%, AC 21%, GT-I, suitable for direct wet and dry,
transplanting, drought, salinity, cold germination BLB, Blast
tolerant
Grainyieldt/ha
Site specific nutrient management (SSNM)
Untungetal(2012)unpubl.

29. Name CoteD’ivoir
Mali
Rwanda
Nigeria
Mozambique
Tanzania
Uganda
Bangladesh
Indonesia
LaoPDR
Pakistan
SriLanka
Vietnam
Philippines
All
HuangHuaZhan 2 1 3 1 3 1 2 1 1 15
Zhongzu14 2 1 1 1 1 1 7
ZH1 2 1 2 1 1 1 1 9
2 1 1 1 1
List of the promising widely adaptable GSR inbreds identified from adaptation
yield trials in SSA, SEA and SA
KCD1 2 1 1 1 1 1 7
RC8 1 2 1 1 1 6
Weed Tolerant 1 1 2 1 2 1 7
HUA-565 2 2 1 5
FFZ 1 1 1 1 1 5
SAGC-4 2 2 1 1 1 7
WX763 2 1 1 1 5
HHZ developed in GAAS is a mega-variety of high yield & superior quality grown in 8 provinces of South & Central
China (Guangdong, Jiangxi, Fujian, Hunan, Hubei, Anhui, Yunan and Guangxi).

30. The complex pedigree of Huang-Hua-Zhan
(HHZ) involving 14 parents

31. S.C.Zhouetal.,unpublishedZ.K.Lietal2012(unpubl.)
Plant type, Panicle and Grain shape & size of HHZ & its immediate parents
P1(FHZ) P2(HXZ) HHZ P1(FHZ) P2(HXZ) HHZ
S.C.Zhouetal.,unpublishedZ.K.Lietal2012(

32. Ch. 1
Ch. 2
Ch. 3
Ch. 4
Ch. 5
Ch. 6
Ch. 7
Ch. 8
Ch. 9
Genomic composition of the HHZ genome based on the re-sequencing data
(From S. C. Zhou et al., unpublished)
Ch. 9
Ch. 10
Ch.11
Chr. 12
Each colored vertical line corresponds to a window of 10 kb. Vertical lines distribute upper side on each chromosome
represent AZ haplotype blocks (red for ≥200kb AZ blocks, light red for <200kb AZ blocks) and QZ haplotype blocks
(blue for ≥200kb QZ blocks and light blue for <200kb QZ blocks). Vertical lines distribute lower side on each
chromosome represent “Stress” related QTL region (light yellow), “Quality” related QTL region (light green) and
“Yield” related QTL region (light purple). Blue and red arrows indicate QZ blocks overlapped with “Yield” related QTL
regions and AZ blocks overlapped with “Quality” related QTL regions, respectively.

33. IRRI-GSR breeding program & strategy
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

34. GSR Introgression Breeding Procedure
8 HHZ BC1F2 populations (08WS)
DT screen SUB screen
15SUBT plants
326 Genotyping/progeny testing for all target traits
Random plants
109DT plants
Yield traits
QTL/Allelic
diversity
discovery
for target
82HY plants
ST screen
120ST plants
326DT screen 311SUB screen326Yield 326ST screen
06WS
08WS
09DS
Ist round
selection
108Preliminary yield trials under DT, low input, NC
Confirming genetic
networks for target
traits and their
genetic relationships
for target
traits
68Promising ILs
47DT ILs 171SUB ILs73HY ILs 78ST ILs
09WS 369Genotyping/progeny testing for all target traits
10DS
10WS/11DS 68 Replicated
yield trials
~80 promising ILs as
parents for designed
QTL pyramiding
2NCT &
29 MET for 11WS
3Demo
2nd round
selection
3rd round
selection
Selections can be
continued if
certain lines
segregating
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

35. GSR Introgression Breeding Procedure
8 HHZ BC1F2 populations (09WS)
DT screen SUB screen
21SUBT plants
637Genotyping/progeny testing for all target traits
Random plants
210DT plants
Yield traits
QTL/Allelic
diversity
discovery
for target
traits
119HY plants
ST screen
287ST plants
DT screen SUB screenYield under
NC & LI
ST screen
06WS
09WS
10DS
221SUB ILs
Ist round
selection
2nd round
selection
Confirming genetic
networks for target
traits and their
genetic relationships
180DT ILs 221SUB ILs420HY&FUE ILs 44ST ILs
10WS 865Genotyping/progeny testing for all target traits
11DS
~80 promising ILs as parents
for designed QTL pyramiding
DT screen SUB screenYield under
NC & LI
ST screen
DT ILs SUB ILsHY&FUE ILs ST ILs
136 PYT11WS
80 RYT12 DS
2 NCT & 11 MET
12DS
2 Demo
selection
3rd round
selection
Selections can
be continued if
certain lines
segregating

36. Target traitsTarget traitsTarget traitsTarget traits
Number of ILsNumber of ILsNumber of ILsNumber of ILs
Produced fromProduced fromProduced fromProduced from
BNBNBNBN
Selected at PYT &Selected at PYT &Selected at PYT &Selected at PYT &
RYTRYTRYTRYT
Nominated toNominated toNominated toNominated to
MET & NCTMET & NCTMET & NCTMET & NCT
Drought tolerance (DT)Drought tolerance (DT)Drought tolerance (DT)Drought tolerance (DT) 613613613613 79797979 21212121
High yield under lowHigh yield under lowHigh yield under lowHigh yield under low----input (LI)input (LI)input (LI)input (LI) 370370370370 27272727 3333
Salinity tolerance (SAL)Salinity tolerance (SAL)Salinity tolerance (SAL)Salinity tolerance (SAL) 502502502502 73737373 18181818
Submergence tolerance (SUB)Submergence tolerance (SUB)Submergence tolerance (SUB)Submergence tolerance (SUB) 128128128128 13131313 2222
High yield under irrigated (Y)High yield under irrigated (Y)High yield under irrigated (Y)High yield under irrigated (Y) 576576576576 100100100100 27272727
DT+LIDT+LIDT+LIDT+LI 246246246246 15151515 2222
DT+SALDT+SALDT+SALDT+SAL 326326326326 19191919 5555
DT+SUBDT+SUBDT+SUBDT+SUB 82828282 6666
DT+YDT+YDT+YDT+Y 382382382382 40404040 11111111
LI+SALLI+SALLI+SALLI+SAL 274274274274 10101010 1111
Multiple abiotic stress tolerant ILs developed from 16 donors into Huanghuazhan
background and nominated to NCT using GSR breeding scheme.GenerationIRRIbredGSRlines
StoodLI+SALLI+SALLI+SALLI+SAL 274274274274 10101010 1111
LI+SUBLI+SUBLI+SUBLI+SUB 38383838 0000
LI+YLI+YLI+YLI+Y 178178178178 1111
SAL+SUBSAL+SUBSAL+SUBSAL+SUB 60606060 9999
SAL+YSAL+YSAL+YSAL+Y 292292292292 42424242 8888
SUB+YSUB+YSUB+YSUB+Y 101101101101 5555 1111
DT+SAL+SUBDT+SAL+SUBDT+SAL+SUBDT+SAL+SUB 35353535 3333 2222
DT+SAL+YDT+SAL+YDT+SAL+YDT+SAL+Y 154154154154 9999
DT+SUB+YDT+SUB+YDT+SUB+YDT+SUB+Y 58585858 3333
LI+SAL+SUBLI+SAL+SUBLI+SAL+SUBLI+SAL+SUB 20202020 0000
LI+SAL+YLI+SAL+YLI+SAL+YLI+SAL+Y 117117117117 0000
LI+SUB+YLI+SUB+YLI+SUB+YLI+SUB+Y 36363636 0000
SAL+SUB+YSAL+SUB+YSAL+SUB+YSAL+SUB+Y 39393939 2222
total:total:total:total: 845845845845 146146146146 40404040
IL=Introgression lines; BN=Backcross Nursery;PYT=Preliminary Yield Trial;RYT=Replicated Yield Trial; NCT=National Cooperative Testing
(Philippines); Multi-environment testing (IRRI)
2ndGenerationIRRIbredGSRlines
Stood
first in
MET
stage 2
under
GRiSP
GSR IR1-8-S6-S3-Y2

37. Yield in any given condition is a
manifestation of several factors
• Genotype
• TPE
• Superior plant ideotype to capture high
photosynthetic efficiency into the relativephotosynthetic efficiency into the relative
larger sink
• Efficient photosynthates mobilization within
plant system
• Resistance to pests & diseases
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

38. Yield Potential :
Highest possible grain yield obtainable under ideal
set of conditions of inputs (water and nutrients)
for a given ecology without insect pest, weed and
diseases pressure
GSR concept on grain yield potential:

39. GSR Yield Concept
Maximize grain yield Increase yield potential
Increase
yield
potential
under
Super Hybrids
Super Rice
Green Super Rice
Maximize grain yield
under limited
supply of nutrients and
water
Increase yield potential
under ample
supply of nutrients and
water
under
sufficient
supply of
nutrients
and water
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

40. Yield barrier -TPE
• High input environment
• Moderate input environment
• Low input environment
Breeding strategy must capture genotypes for
each conditions separately rather than
identifying one genotype for all conditions
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

41. Yield
Stability-
Specific
Selection
strategy-
Resilience
Plant
architecture
designed for
specific rice
ecology
Specific
adaptation
traits
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

43. GSR IR1-5-S14-S2-Y2

45. IRRI bred first generation of high yielding GSR materials
5.2
5.6
5.0
5.3
6.9
6.4
5.6
6.3
5.3
7.0
5.9
6.1
4.8
7.2
5.8 5.9
5.3
6.9
5.3
5.8
5.4
6.8
4.7
5.6
4.7
5.8
4.0
4.8
4.00
5.00
6.00
7.00
8.00
3-Season Data of HHZ Lines nominated to NCT
0.00
1.00
2.00
3.00
4.00
2010 WS 2011 DS 2011 WS Mean
Seasonal Mean HHZ 12-SAL2-Y3-Y2 HHZ 8-SAL6-SAL3-Y2
HHZ 5-SAL6-SAL3-DT1 HHZ 5-SAL14-SAL2-Y2 NSIC Rc222
APO IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

46. IRRI bred GSR lines nominated to NCT Philippines

47. GSR IR1-8-S6-S3-Y2
High yielding under irrigated with moderate multiple abiotic stress tolerance GSR line with good cooking
quality suitable for rainfed and irrigated lowlands.

48. Designation
Grain Yield (t/ha)
Mean
over
seasons
%
over
IR72
%
over
NSIC
Rc158
2010WS 2011DS
HHZ8-SAL6-SAL3-Y2 6.55ab 8.0ab 7.28 10.56 12.27
Mestizo7 (Hybrid) 5.68 bcde 8.7a 7.19 9.27 10.96
HHZ12-DT10-SAL1-DT1 6.75a 7.2 bcde 6.98 6.00 7.64
IR83142-B-7-B-B 6.00 abcde 7.6 bc 6.80 3.34 4.94
HHZ5-SAL10-DT1-DT1 6.14abcd 7.4 bcd 6.77 2.89 4.48
IR72 5.96abcde 7.2 cde 6.58 0.00 1.54
Performance of IRRI bred GSR High Yield Potential
Varieties under Irrigated Conditions
HHZ5-DT8-DT1-Y1 5.55 cde 7.6 bc 6.58 -0.08 1.47
HHZ8-SAL12-Y2-DT1 6.43abc 6.7 def 6.57 -0.23 1.31
NSICRc158 5.86 bcde 7.1 cdef 6.48 -1.52 0.00
HHZ12-Y4-DT1-Y1 5.57cde 7.1 cdef 6.34 -3.72 -2.24
IR83142-B-19-B 5.12 e 7.5 bcd 6.31 -4.10 -2.62
IR83142-B-57-B 5.48 de 7.1 cdef 6.29 -4.41 -2.93
IR83143-B-21-B 5.16 e 7.2 cde 6.18 -6.08 -4.63
HHZ8-SAL9-DT2-Y1 5.78 bcde 6.4 defg 6.09 -7.45 -6.02
HHZ5-SAL10-DT3-Y2 5.69 bcde 6.3 fg 6.00 -8.89 -7.48
HHZ5-SAL10-DT2-DT1 5.47 de 6.0 g 5.74 -12.84 -11.50
Higher HI, spikelets per panicle;panicles per sqm;total spikelets per sqm, CGR
Plot size: 30sqm SSNM High yielding, IG+DT+SAL+SUB+Low input SSNM
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

49. RYT IRRI 2011 WS Irrigated Low-input Rainfed
BC1F8 HHZ 8-SAL6-SAL3-Y2 5.88 3.90 5.24
BC1F8 HHZ 8-SAL6-SAL3-Y1 4.41 2.99 3.46
BC1F7
HHZ 8-SAL6-SAL3-
SAL1
3.95 3.00 4.38
Huang-Hua-Zhan 3.09 2.92 3.56
NSIC Rc222 4.71 2.95 5.17
Round 3 IRRI 2009 WS IG RF SAL SUB
BC1F4 HHZ 8-SAL6-SAL3 4.40 4.25 10.00 55.56
IRRI bred GSR IR1-8-S6-S3-Y2 Breeding History
Round 1 IRRI 2008 WS IG RF SAL SUB
BC1F2 HHZ 8 9 SPS 13 SPS 36 SPS 0 SPS
Round 2 IRRI 2009 DS IG DT SAL SUB
BC1F3 HHZ 8-SAL6 5.58 2.25 41.67 36.36
>Promoted to MET2 (2012 DS)
Combines earliness (105d), high yield inCombines earliness (105d), high yield in irrigated, low input, drought, salinity, submergence,irrigated, low input, drought, salinity, submergence, directdirect
seeded, transplanted, resistant to blast, BLBseeded, transplanted, resistant to blast, BLB
NSIC Rc222 4.71 2.95 5.17
Apo 3.98 3.47 3.60
MET1 2011 DS
IRRI Philrice Philrice Philrice
Los
Banos
CES Isabela Agusan
BC1F7 HHZ 8-SAL6-SAL3-Y2 3.48 8.72 7.74 0.83
RYT IRRI 2011 DS Irrigated Low-input Drought
BC1F7 HHZ 8-SAL6-SAL3-Y2 7.00 3.11 0.81
BC1F7 HHZ 8-SAL6-SAL3-Y1 6.05 2.75 0.86
BC1F6
HHZ 8-SAL6-SAL3-
SAL1
5.90 2.52 0.95
Huang-Hua-Zhan 6.39 2.02 0.20
NSIC Rc222 6.77 2.93 0.46
Apo 5.78 2.88 1.35
PYT IRRI 2010 WS Irrigated Low-input Rainfed 75% N
BC1F6 HHZ 8-SAL6-SAL3-Y2 5.32 4.17 5.32 5.09
BC1F6 HHZ 8-SAL6-SAL3-Y1 5.59 3.31 4.75 4.50
BC1F5
HHZ 8-SAL6-SAL3-
SAL1
4.67 3.94 5.57 4.34
Huang-Hua-Zhan 4.89 4.20 4.42 5.37
NSIC Rc222 5.35 4.78 4.94 4.16
Apo 4.71 4.77 5.52 4.48
PYT IRRI 2010 DS Irrigated Low-input Drought
BC1F5 HHZ 5 SAL14-SAL2-Y2 7.75 2.77 2.11
BC1F5 HHZ 8-SAL6-SAL3-Y1 6.66 1.94 2.45
Huang-Hua-Zhan 7.58 3.31 2.49
IR 72 6.99 3.08 2.53
Apo 6.94 3.23 3.07 > Submitted to NCT (Transplanted Rice Category)
> Submitted to MET1 (2011 DS)

50. Mean yield (t/ha) of IRRI bred GSR entries
in MET Stage 2, 2012DS
MET Entry Fixed Name
Location Ave.
yieldNE Isabela Agusan Bohol IRRI
MT4004 HHZ 8-SAL 6-SAL 3-Y2 8.86 7.20 4.32 5.20 5.97 6.31
MT4008 HHZ 1-Y4-Y1 8.22 7.21 3.74 4.81 4.97 5.79
MT4017 HHZ 8-SAL9-DT 2-Y1 7.52 6.39 3.94 4.75 5.03 5.53
MT4018 HHZ 12-Y 4-DT 1-Y 1 7.99 5.44 3.95 2.76 5.81 5.19
MT4019 HHZ 12-DT 10-SAL 1-DT 1 8.04 5.45 4.58 4.94 5.12 5.62
MT4901 IRRI 104 7.40 5.98 4.06 4.91 5.74 5.61
GSR IR1-8-S6-S3-Y2 = HHZ8-SAL6-SAL3-Y2 is irrigated highest yielder under DS;
tolerant to drought(~ -75 kPascal at reproductive stage),salinity(6-10dSm1), submergence (14d
submergence 4.3 PACP score). It is nutrient and radiation use efficient line
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI
Redona- MET Coordinator
MT4901 IRRI 104 7.40 5.98 4.06 4.91 5.74 5.61
MT4904 IRRI 105 8.18 6.36 3.75 4.66 4.86 5.56
MT4902 IRRI 123 7.26 5.48 4.57 4.79 4.90 5.90
MT5211 IRRI 153 6.76 5.86 4.37 5.85 5.48 5.66
MT4903 IRRI 154 9.06 5.35 4.51 4.82 4.98 5.74

51. Rank Treatments means
DMRT
Groups
Advantage
over
IR 63307-4B-
4-3 (ST Ck)
Advantage
over
NSIC Rc184
(NCT-ST Ck)
1 HHZ 1-Y4-Y1 5.60 a 7.5% 18.0%
2 HHZ 5-SAL14-SAL2-Y2 5.38 ab 3.2% 13.3%
3 IR 63307-4B-4-3 (ST Ck) 5.21 abc 0.0% 9.8%
4 IR 83140-B-11-B 5.13 abc -1.5% 8.1%
Salinity Trial at Infanta, Quezon(2012DS, Site EC ~6)
4 IR 83140-B-11-B 5.13 abc -1.5% 8.1%
5 HHZ 5-SAL8-DT3-SUB1 5.11 abc -2.0% 7.5%
6 HHZ 8-SAL6-SAL3-Y2 5.09 abc -2.4% 7.1%
7 HHZ 12-DT10-SAL1-DT1 5.06 abc -2.9% 6.6%
8 NSIC Rc184 (NCT-ST Ck) 4.75 bcd -8.9% 0.0%
9 IR 83140-B-28-B 4.65 cd -10.8% -2.1%
10 IR 84675-58-4-1-B-B 4.29 d -17.8% -9.7%
11 HHZ 5-Y3-SAL2-SUB1 4.19 d -19.6% -11.7%
Seeding Date:20-Dec-11 3 replications
Transplanting date:19-Jan-12 12.75sqm
Fertilizer Rate:120-40-40 (N-P-K)
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

52. MULTIPLE ABIOTIC TOLERANT IRRI BRED GSR LINES SCREENED FOR SUBMERGENCE
TOLERANCE
Independently screened By
BERT COLLARD during
DS2012 in submergence
tanks at IRRI
Checks average over 8-10
reps while all other lines
averaged over three
replications
Triple traits like
DT-SUB-SALDT-SUB-SAL
combination-highlighted
in orange;
RED highlighted arrow has
the mean highest yielder
over DS&WS
W142 highlighted in blue is
in NPT background and has
AGT+SUBT+SAL and
moderate DT

53. 2.00
4.00
6.00
8.00
Victoria
Yieldt/ha PVS-GSR trials in Laguna province, Philippines
harvested yield (t/ha) DS2012
(harvested plot size ~700sqm and twice replicated)
GSR IR1-8-S6-S3-Y2 gave mean of 26.4% advantage over NSIC222 at two PVS sites tested in Laguna
0.00
2.00 Victoria
Bae
Cultivar Mean
Yieldt/ha
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

54. Rapid Visco Analyzer (RVA) Pasting properties of GSR lines in IR64 and HHZ RP backgrounds-
suitable for varied consumers with different taste preferences
3000
4000
5000
6000
Viscosity,cP
60
80
100
120
Temperature
1 2
3 4
5 6
7 8
9 10
11 12
13 14
15 16
17 18
-1000
0
1000
2000
0 100 200 300 400 500 600 700 800
Time, sec
Viscosity,cP
0
20
40
Temperature
19 20
21 22
23 24
25 26
27 28
29 30
31 32
33 34
35 36
AC=14.5-31.6%; GT=H-I-L; Protein=7.8-11.2

55. D
GSR IR1-12-D10-S1-D1
AROMATIC-Suitable -Direct seeding & Irrigated conditions, good cooking
quality (AC24%,GT-L, Protein 9.4%) Drought and Salinity tolerant

56. GSR IR1-12-D10-S1-D1 PVS trials (40 farmers) at Puypuy, Laguna –ranked best over
farmer’s check NSiC214 during WS2011 with preference score=0.118 against -
0.0063(NSiC214)
High Yielding, Suitable for Rainfed & Irrigated lowland
conditions, Aromatic, good cooking quality (AC 24%,
GT-L, Protein 9.4%) with moderate tolerance to Salinity

57. Selection Strategy Favors Resilience
Conventional
breeding strategy
IRRI-GSR breeding
strategy
IRRIIRRIIRRIIRRIIRRIIRRIIRRIIRRI

58. ResilientResilient
GSR products

65. No.
Populat
ion
No.
of ILs
HY LI DT RF ST SubT
1 HHZ 5 13 4 6 3 2 7 5
2 HHZ 8 6 2 2 0 3 5 2
3 HHZ 9 1 0 0 0 0 0 1
Number of HHZ ILs according to traits.
3 HHZ 9 1 0 0 0 0 0 1
4 HHZ 11 4 0 1 1 0 2 3
5 HHZ 12 6 3 3 4 0 1 0
6 HHZ 15 1 0 1 0 0 1 0
6 HHZ 17 3 0 1 0 0 2 0
Total: 34 9 14 8 5 18 11

66. Criteria for DQP
Best HHZ ILs from Batch 1 for Design QTL
Pyramiding (DQP)
•Lines that performed well in at least 2 season
from 4 seasons (2010DS, 2010WS, 2011DS, &
2011WS) of testing for irrigated (HY), low-input
(LI), drought (DT), and Rainfed (RF) conditions;(LI), drought (DT), and Rainfed (RF) conditions;
•Salinity tolerant and submergence tolerant
lines in second round of screening (BC1F3,
2009DS) that has been confirmed in third round
of screening (BC1F4, 2009WS).

67. No.
Populat
ion
No. of
ILs
1 trait
2
traits
3
traits
4
traits
5
traits
1 HHZ 5 13 5 5 1 2 0
2 HHZ 8 6 4 0 0 1 1
3 HHZ 9 1 1 0 0 0 0
Number of HHZ ILs with multiple traits
3 HHZ 9 1 1 0 0 0 0
4 HHZ 11 4 2 1 1 0 0
5 HHZ 12 6 1 5 0 0 0
6 HHZ 15 1 0 1 0 0 0
7 HHZ 17 3 3 0 0 0 0
Total: 34 16 12 2 3 1

68. IL2- HY, DT, STIL1- HY, DT, SUB X
F1
F2
DQP strategy for combining high yield(HY), drought tolerance(DT), salinity tolerance(ST)
and submergence tolerance(SUBT) using the promising ILs as parental lines
Designed QTL pyramiding (DQP)
DT screen SUB screen
SUBT plants
SNP Genotyping/progeny testing for all target traits
Random plants
DT plants
Yield traits
Group genotypes
for different traits
and their NILs
HY plants
ST screen
ST plants
New HYVs cultivars
with DT,ST, SUBT
for NCYT and PVS
Promising
PLs for yield
Confirm genetic networks for
different target traits and
their genetic relationship
Farmers

69. GSR Technology
GSR
Technology
ILBreeding,
DQP & PDLs
Ideal RP BG
Ecosystem based approach
GSR
500 donors
56 RPs
Screening of
released GSR
materials under
target ecosystems
Screening of already
developed PDLs for
abiotic stresses
DT, ST, SUB, LI in the
target ecosystems
DQP for a trait &
ecosystem related
traits
ILs, PDLs, DQP with
adaptable RP BG for
different target
ecosystem
Increase in success rate to develop highly
adaptable genotypes for a given ecosystem
First Phase
2009-2012
Second Phase
2012-2018
Ecosystem based approach

70. An additional ton of rice in the rain-fed and irrigated
lowlands will change the livelihoods of millions of resource
poor farmers from the clutches of poverty and sustained
income source to prosper….
THANKS
GSR IR1-12-D10-S1-D1 –Aromatic & High Yielding
Suitable for Direct seeding & Irrigated conditions, good cooking
quality (AC24%,GT-L, Protein 9.4%) + Drought and Salinity tolerant

71. Acknowledgements & Thanks:
IRRI-CAAS-BMGF
Dr Zhikang Li Director GSR project
GSR National Coordinators (Asia)
Drs Tuat, Untung, Helaluddin, Somphet, Nimal, Riaz, Arif, Makara,
Two public/NGO sectors:
Dr W.Xu (Boshima-SS,IDO) & Dr Sirajul Islam(BRAC)
Dr C.X Mao GSR Training Consultant (CAAS-GAAS)Dr C.X Mao GSR Training Consultant (CAAS-GAAS)
GSR-CAAS team: Drs Z. Li, Gao, Xu, Judy, Fu, Yu & others
IRRI GSR team: Drs Nollie, Glenn, Choi, Redonna,Val Pede,Andy,Krishna,Wang,Tao
GSR-MBL team: Gelo; Corine, Lolit, Denver, Macdo, Cenon, Keno, Deo, Eugene
Visiting Research Fellows: Drs Ma,
PhD: Zilhas, Meng; MS:Nina OJT: Shahana, Dilruba
GSR Project Adm.: Pauline; Secretarial Assistance: Badett
“Cooperation & Collaboration makes the world a smaller place”