I-TASSER is an online server that uses an iterative threading assembly approach to generate protein structure and function predictions from amino acid sequences. It follows a sequence-to-structure-to-function paradigm using template identification, fragment structure reassembly simulations, model construction, and structure-based function interpretation. I-TASSER has been ranked the best server for protein structure and function prediction in CASP experiments.

1. Presentation By : Anjali Bajaj

2. I-TASSER stands for Iterative Threading ASSEmbly Refinement.
A Protocol for Computer-Based Protein Structure and Function
Prediction
A unified platform for automated protein structure
I-TASSER Server is an internet service for protein
structure and function predictions. It allows academic
users to automatically generate high-quality
predictions of 3D structure and biological function
of protein molecules from their amino acid
sequences.
It has been ranked as the best server for protein structure and function
prediction in CASP Experiments.

3. • It follows the sequence-to-structure-to-function paradigm.
• Procedure for structure and function modeling involves :
Template identification by LOMETS
Fragment Structure reassembly by
replica-exchange Monte-Carlo simulations
Atomic level model
construction of structure refinement
Structure based function
interpretations using COFACTOR

4. How to submit a query sequence?
Step 1: Open I-TASSER Server

5. Step 2: Enter Sequence and mandatory fields then Run I-TASSER

6. Step 3: Acknowledgement from I-TASSER Server

7. Results

14. I-Tasser helps in:
• Protein Structure Prediction
• Struture-based Function Annotation
• Protein Design
• Modeling of G Protein-Coupled Receptor and Ligand-Receptor Interactions
• Amyloid Diseases and Fiber Aggregation
• Modeling of Protein-Protein Interactions
• RNA Alternative Splicing
• Ligand Screening and Structure-Based Drug Design