I-TASSER is an online server that uses a unified platform to automatically generate high-quality predictions of 3D protein structures and their biological functions from amino acid sequences. It follows a sequence-to-structure-to-function paradigm, using multiple algorithms like LOMETS template identification, fragment structure reassembly through simulations, atomic model construction and structure refinement to build models and predict functions through COFACTOR analysis. Users can submit protein sequences to the I-TASSER server through a simple three step process to obtain structure and function predictions and results.

1. Presentation By : Anjali Bajaj

2. A Protocol for Computer-Based Protein Structure and Function
Prediction
A unified platform for automated protein structure
I-TASSER Server is an internet service for protein
structure and function predictions. It allows academic
users to automatically generate high-quality
predictions of 3D structure and biological function
of protein molecules from their amino acid
sequences.

3. I-Tasser helps in:
• Protein Structure Prediction
• Struture-based Function Annotation
• Protein Design
• Modeling of G Protein-Coupled Receptor and Ligand-Receptor Interactions
• Amyloid Diseases and Fiber Aggregation
• Modeling of Protein-Protein Interactions
• RNA Alternative Splicing
• Ligand Screening and Structure-Based Drug Design

4. • It follows the sequence-to-structure-to-function paradigm.
• Procedure for structure and function modeling involves :
Template identification by LOMETS
Fragment Structure reassembly by
replica-exchange Monte-Carlo simulations
Atomic level model
construction of structure refinement
Structure based function
interpretations using COFACTOR

5. How to submit a query sequence?
Step 1: Open I-TASSER Server

6. Step 2: Enter Sequence and mandatory fields then Run I-TASSER

7. Step 3: Acknowledgement from I-TASSER Server

8. Results

15. Conclusion