Recombinant antibodies are antibody fragments generated by using recombinant antibody coding genes as a source and display technology, delivering high reproducibility, specificity and scalability. Unlike monoclonal antibodies (mAbs) which are produced using traditional hybridoma technologies, rAbs do not need hybridomas and animals in the production process if you only use synthetic genes.
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
Hello. The slide is produced by Creative Biolabs who has extensive experience in therapeutic antibody and recombinant antibody production. In this video, we will give you a whole understanding to the conventional antibody, recombinant antibody and their basic information and expression.
It includes general introduction to antibodies; Monoclonal antibodies; comparison between Polyclonal & Monoclonal antibodies; Hybridoma Technology & Hyridoma Selection; advantages & disadvantages of mABs; Applications of mABs; Recombinant Monoclonal antibodies production through Antibody Engineering.
Recombinant Antibody Production: Current Methods and a Novel Antibody Generat...InsideScientific
Dr. Yuning Chen discusses strategies involved in recombinant antibody production and introduces a novel antibody expression platform to facilitate antibody library generation.
Antibodies are bio-macromolecules that bind to their corresponding antigens with exceptional specificity and affinity. They are the foundation of modern biopharmaceuticals and are widely used as therapeutics, diagnostic agents, and tools for biomedical research. Antibodies are derived from methods such as hybridoma, phage-display, and B cell sorting, and produced mainly via recombinant expression.
In this talk, Dr. Yuning Chen from Sino Biological reviews strategies involved in recombinant antibody production, particularly aspects such as construct design, culture condition optimization, and purification methods to provide the audience with a gateway to the field of recombinant antibody expression, technology, and optimization. He also introduces a novel high-throughput antibody expression platform to facilitate antibody library generation and the identification of suitable candidates for further development.
Key Topics Include:
- What are antibodies and what is their role in modern biotechnology?
- Recombinant antibody expression: host systems and general workflow
- Strategies for recombinant antibody construct design
- Methods for optimal recombinant antibody expression and purification
- Applications of a recombinant antibody generation platform for research and therapeutic antibody production
Recombinant Antibody Overview I - Creative BiolabsCreative-Biolabs
Hello. The slide is produced by Creative Biolabs who has extensive experience in therapeutic antibody and recombinant antibody production. In this video, we will give you a whole understanding to the conventional antibody, recombinant antibody and their basic information and expression.
It includes general introduction to antibodies; Monoclonal antibodies; comparison between Polyclonal & Monoclonal antibodies; Hybridoma Technology & Hyridoma Selection; advantages & disadvantages of mABs; Applications of mABs; Recombinant Monoclonal antibodies production through Antibody Engineering.
Recombinant Antibody Production: Current Methods and a Novel Antibody Generat...InsideScientific
Dr. Yuning Chen discusses strategies involved in recombinant antibody production and introduces a novel antibody expression platform to facilitate antibody library generation.
Antibodies are bio-macromolecules that bind to their corresponding antigens with exceptional specificity and affinity. They are the foundation of modern biopharmaceuticals and are widely used as therapeutics, diagnostic agents, and tools for biomedical research. Antibodies are derived from methods such as hybridoma, phage-display, and B cell sorting, and produced mainly via recombinant expression.
In this talk, Dr. Yuning Chen from Sino Biological reviews strategies involved in recombinant antibody production, particularly aspects such as construct design, culture condition optimization, and purification methods to provide the audience with a gateway to the field of recombinant antibody expression, technology, and optimization. He also introduces a novel high-throughput antibody expression platform to facilitate antibody library generation and the identification of suitable candidates for further development.
Key Topics Include:
- What are antibodies and what is their role in modern biotechnology?
- Recombinant antibody expression: host systems and general workflow
- Strategies for recombinant antibody construct design
- Methods for optimal recombinant antibody expression and purification
- Applications of a recombinant antibody generation platform for research and therapeutic antibody production
History of immunology grew out of the observation that individuals who have recovered from certain infectious diseases were thereafter protected from the disease.
In this ppt one can know the diiferent softwares and several online servers for the epitope mapping.This mapping helpful for production of different vaccines and antibodies.
This presentation covers a general introduction to expression vector, its components, types, and its application. Then it covers some of the expression system with examples.
What are an expression vector? Detailed description of plant gene structure. Plant expression vector systems are generally consists of Ri and Ti plasmids.
The other vectors which are generally used are DNA and RNA viruses.
Antibodies are immune system-related proteins called immunoglobulins. Each antibody consists of four polypeptides– two heavy chains and two light chains joined to form a "Y" shaped molecule. ... This variable region, composed of 110-130 amino acids, give the antibody its specificity for binding antigen.
LECTURE FOR MBBS, IMMUNOLOGY
ANTIBODY
React specifically & in some observable manner with Ag which stimulated their production
globulins produced in response to antigenic stimulation
History of immunology grew out of the observation that individuals who have recovered from certain infectious diseases were thereafter protected from the disease.
In this ppt one can know the diiferent softwares and several online servers for the epitope mapping.This mapping helpful for production of different vaccines and antibodies.
This presentation covers a general introduction to expression vector, its components, types, and its application. Then it covers some of the expression system with examples.
What are an expression vector? Detailed description of plant gene structure. Plant expression vector systems are generally consists of Ri and Ti plasmids.
The other vectors which are generally used are DNA and RNA viruses.
Antibodies are immune system-related proteins called immunoglobulins. Each antibody consists of four polypeptides– two heavy chains and two light chains joined to form a "Y" shaped molecule. ... This variable region, composed of 110-130 amino acids, give the antibody its specificity for binding antigen.
LECTURE FOR MBBS, IMMUNOLOGY
ANTIBODY
React specifically & in some observable manner with Ag which stimulated their production
globulins produced in response to antigenic stimulation
An antibody (Ab), also known as an immunoglobulin (Ig), is a Y-shaped protein produced mainly by B- plasma cells that is used to neutralize pathogens such as bacteria, viruses etc.These slides will reflect the introduction, structure and types of antibodies.
Immunology is the study of the immune system and is a very important branch of the medical and biological sciences. The immune system protects us from infection through
Design of Organ-On-A-Chip - Creative Biolabs.pptxCreative-Biolabs
Creative Biolabs has developed an extensive microfluidic technology platform, offering customers one-stop services covering all aspects of microfluidic research and evaluation. This includes the design and manufacturing of organ-on-a-chip (OOC) systems, as well as personalized, customized solutions tailored to individual needs.
This slide briefly introduces our OOC design concepts. If you require further details, products, and services related to OOC, please follow us to stay updated and informed.
Introduction of Organ-On-A-Chip - Creative BiolabsCreative-Biolabs
Creative Biolabs has established a complete microfluidic technology platform. We provide customers with one-stop services in all aspects of microfluidic research and evaluation, including Organ-on-a-Chip design and manufacturing and personalized, customized solutions.
This slide provides a brief introduction to OOC. If you need more information, products, and services about OOC, please follow us to stay informed.
Advances in Oncolytic Virotherapy - Creative BiolabsCreative-Biolabs
Oncolytic virotherapy, an innovative approach leverages the natural ability of viruses to infect and kill tumor cells, offering a beacon of hope for patients battling cancer. Our journey through this domain uncovers the mechanisms of action of oncolytic viruses, explores representative examples, discusses strategic modifications and combinations with other therapies, addresses challenges, and reviews the clinical status of this cutting-edge treatment.
This slide provides a comprehensive overview of lipid nanoparticle-based mRNA Vaccine development, detailing the technological timeline, the 2023 Nobel Prize-winning science behind the vaccines, and the specifics of COVID-19 vaccine candidates BNT162b2 and mRNA-1273. It also explores the advantages of liposomes in mRNA delivery, the intricate mechanisms of LNP-based vaccines, their therapeutic potential beyond COVID-19, and the rigorous development process. Creative Biolabs supports these innovations with specialized services and products, pushing the boundaries of medical science.
After decades of development and innovation, Creative Biolabs is committed to demonstrating our strong expertise and capabilities in the field of ribosome research, as well as expanding our research and service capabilities to multiple fields. This five-part slide we will briefly introduce ribosome analysis technology, polysome profiling, ribosome profiling, ribosome affinity purification, and highlight the ribosome analysis solutions provided by Creative Biolabs.
Advances in CAR-T Cell Therapy - Creative BiolabsCreative-Biolabs
Various barriers restrict the efficacy and/or prevent the widespread use of CAR-T cell therapies in these patients as well as in those with other diseases, particularly solid tumors. The evolution of CAR designs beyond conventional structures will be necessary to address these limitations and expand the use of CAR-T cells to a wider range of diseases. In this presentation, we discuss the progress in the development of innovative designs for new CAR-T cell products to increase and expand the clinical benefits of these treatments for patients with various cancers, and we explore the potential of CAR-T cell therapies in addressing diseases beyond cancer.
Vaccine for Cancer Immunotherapy - Creative BiolabsCreative-Biolabs
Cancer vaccines are designed to stimulate anti-tumor immunity through active immunization with tumor antigens and has long been envisioned as a key tool of effective cancer immunotherapy. Today we will go through the history, formulations, mechanisms of action, and clinical status of cancer vaccines. Creative Biolabs has been effectively supporting the cancer vaccine industry with our one-stop solutions for cancer vaccine discovery and development. We will accommodate the specific properties and clinical purpose of your vaccine candidate, and take careful scientific considerations to ensure the most appropriate solutions to develop your projects.
PROTAC Technology in Tumor Targeted Therapy - Creative BiolabsCreative-Biolabs
Today, we will explore the protac technology applied in tumor-targeted therapy. The following will be presented to you, such as a brief introduction to Protac, the mechanism of action, the advantages and disadvantages of protac as a drug, and the core content: the application of protac technology in tumor therapy. And the last part, the protac solutions provided by Creative Biolabs. If you have any questions about the PROCT development, please email us.
Email: info@creative-biolabs.com
Macrophages as Targets in Cancer Immunotherapy - Creative BiolabsCreative-Biolabs
Due to the limitations and shortages of traditional cancer treatments, immunotherapy has become the most promising cancer treatment. Various cancer immunotherapy strategies have emerged. These include adoptive cellular immunotherapy, tumor vaccines, antibodies, immune checkpoint inhibitors, and small molecule inhibitors. Although most of these strategies are not meant to target macrophages directly or originally, macrophages contribute significantly to the final outcomes.
As a CRO company, Creative Biolabs offers first-in-class macrophage therapeutic development services. Please don’t hesitate to contact us if you are interested in our services or if you have any questions.
Monkeypox Drug and Vaccine Discovery - Creative BiolabsCreative-Biolabs
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
Over the past decades, Creative Biolabs has become a leader in antibody drug discovery and manufacturing, providing high quality services to clients in academia and industry around the world. Now, we are able to provide solutions to accelerate drug discovery and development by deploying artificial intelligence technologies. Here, we will briefly introduce the basics of AI-augmented drug discovery, algorithm classification, common AI models, and related services.
Creative Biolabs has extensive experience in coronavirus research and can provide a comprehensive range of high-quality services and products related to SARS-CoV-2 and its variants.
This slide provides a brief introduction to the SARS-CoV-2 variant, Omicron. If you need more information, products, and services about Omicron, please follow us to stay up-to-date.
Hello, everyone. This is Creative Biolabs. Today we will learn about myeloid leukemia vaccines. Our contents here include the Introduction to Myeloid Leukemia, Current Treatment of Myeloid Leukemia, Obstacles to Cancer Vaccine, Targets of Myeloid Leukemia Vaccine, Myeloid Leukemia vaccine types, Examples of Myeloid Leukemia Vaccines, and related services you can find at Creative Biolabs.
Immune Cell Migration in Cancer and Immunotherapy - Creative BiolabsCreative-Biolabs
There is a growing appreciation that “immune contexture” has a significant impact on the clinical outcome for cancer patients. Thus, immunotherapies targeting immune cell migration can be developed to modulate the immune contexture in tumor microenvironment. In this slide you will learn about immune cell migration and its application in cancer immunotherapy development.
As a CRO company, Creative Biolabs offers immunotherapy preclinical development services, and a full range of T cell services. Please don’t hesitate to contact us if you have any questions.
Liposomal Delivery Systems in Cancer Therapy - Creative BiolabsCreative-Biolabs
The lipid-based drug delivery system is a newly developed drug carrier that can be applied for various cancer-targeted treatments with many superiorities. This video briefly introduces various types of liposomes, the principles of liposomal drug delivery systems for cancer therapy, and liposome development services and products provided by Creative Biolabs.
A brief introduction to non-IgG antibody - Creative BiolabsCreative-Biolabs
Creative Biolabs has been a leading bio-company in immunotherapy and pharmaceuticals, especially in non-IgG antibody development, through more than a decade of exploration and expansion. Here, we will give a brief introduction to non-IgG antibodies, understand the characteristics and functions of various antibodies, and show some related products and services of Creative Biolabs.
This slide is about the basics of mRNA-based therapy. The content includes: definition of mRNA, timeline of mRNA therapeutics, action mechanism and development strategies of mRNA drugs, therapeutic mRNA applications, and the related services provided by Creative Biolabs.
Conventional monoclonal antibodies cannot reach adequate therapeutic effects in some cases. Enhancing the effector functions is one of the major strategies for the development of engineered monoclonal antibodies to make up for the deficiencies. Here, we will discuss the development of effector function-enhanced antibodies, and the solutions provided by Creative Biolabs.
Stem Cells in A New Era of Cell based Therapies - Creative BiolabsCreative-Biolabs
A stem cell can replicate itself or differentiate into cells that carry out the specific functions of the body. The application of stem cells in regenerative medicine and disease therapeutics is one of the most exciting advances in medical science today. In cell-based therapies, stem cells may play two roles. The first role is as drug-delivery vehicles. The second role is as therapeutic agents themselves. Stem cells also offer opportunities for scientific advances that go far beyond cell-based therapies. Creative Biolabs is dedicated to facilitate the research of stem cells in both basic science and therapeutics development. Please contact us if you are interested in our services or products.
Anti Virus Biomolecular Discovery - Creative BiolabsCreative-Biolabs
Creative Biolabs is the leading custom service provider in antibody development and engineering. Our scientists bring state-of-the-art technology to support functional antibody and peptide discovery services against viruses. Here, we will introduce the virus and its pathogenic mechanism in detail. At the same time, you will learn how to obtain functional anti-virus antibodies and peptides through our technology platform.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
3. Antibody Structure
Be composed of:
• Two heavy chains
• Two light chains
Two structural region:
• Constant region
• Variable region
4. Five Isotypes of Antibody
Antibody Isotype Type of Heavy Chain Sub-isotypes Structure Function
IgA α IgA1, IgA2 Monomer Tetramer
Preventing pathogens invading into tissues
from epiderm
IgD δ N/A Monomer
Activating basophils and mast cells to produce
antimicrobial factors
IgE ε N/A Monomer
Interacting with allergens, facilitating histamine
releasing from mast cells and basophils,
preventing worms infection
IgG γ
IgG1, IgG2, IgG3,
IgG4
Monomer
The major antibody fighting against
pathogens
IgM μ N/A
Monomer Provide early elimination of pathogens before
5. Antibody Isotypes
• Predominant in secretions
• Monomeric form in blood
IgA
IgD
• Function is unclear
• Receptor on B cells
IgG
• 4 subclasses due to variation in γ
• serum concentration:
• IgG1>IgG2>IgG3>IgG4
IgE
• Bound to surface of
mast cells/basophils
• Most efficient at antigen-binding
• Monomeric IgM as mIgM on B cells
• Joined by J chain
IgM
6. Recombinant Antibody Production
B cell repertoire
Antibody gene
Gene Clone
Recombinant DNA
Phage display
Yeast display
Ribosome display
Recombinant
antibody
Fab fragment
ScFv
Single domain
antibody (SdAb)
7. Three Common Recombinant Antibody Fragment
VH, VL and linker
without ch1
Camelid antibody
Shark antibodySingle domain
antibody(sdAb)
scFv
Fab
Papain
8. Advantage & Disadvantage of Recombinant Antibody
Recombinant Antibody
Smaller size monovalency;
Ease of engineering & manufacture;
Improved tissue penetration;
Lack of potentially deleterious Fc effector
function.
Conventional Antibody
Lower antibody yield;
Highly training & experienced technician.
9. Recombinant Antibody
Provided
by Creative Biolabs
Products:
• scFv Fragment Antibodies
• Fab Fragment Antibodies
• Single Domain Antibodies
• Rabbit Monoclonal Antibodies
Services:
• Recombinant Antibody Construction
• Recombinant Antibody Purification & Expression
10. Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806 Fax: 1-631-614-7828
Email: info@creative-biolabs.com
Web: www.creative-biolabs.com
Contact Us for Recombinant Antibody
