Vericillium wilt of olive tree (Olea europaea L.) is one of the most important diseases of olive plantations worldwide. Four distinguishable native AMF species (Paraglomus occultum (C. Walker), Glomus etunicatum W.N. Becker & Gerd. Glomus fasciculatum (Thaxt.) Gerd. & Trappe and Glomus clarum T.H. Nicolson & N.C. Schenck) were morphologically identified. The effectiveness of these native AMF fungi as inoculants for two target varieties of olive (Roghani and Zard), currently used in Iran was assessed. The current study in interaction of AMF fungi with Verticillium dahliae kleb. of olive inoculated with four different AMF fungi were used to suppress Verticillium wilt under greenhouse conditions. The fresh and dry weight of shoots and roots and the rate of increase in plant height and leaf number were significantly greater for olive seedlings inoculated with both AMF and Verticillium. No significant differences were observed between growth of olive seedlings inoculated with V. dahliae and control. This study supports the need to explore and exploit the natural diversity of AMF fungi as a starting point to formulate inoculants to be applied during the commercial nursery production of olive varieties.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Effect of incubation temperature on lesion diameter of Penicillium expansum o...Innspub Net
Current research work was conducted to determine impact of various genotypes and heat on shelf life of apple. Study was carried out in vitro. Effect of temperature on lesion diameter of fungal growth in most prominent apple fruit varieties growing in Pakistan was assessed. Four temperature (5, 15, 25 and 35°C) treatments were provided to fruits of each variety after inoculation with spore suspension adjusted at 106spores/ml and growth of P. expansum was measured for 12 days at 3 days interval. Pathogen showed maximum growth (23mm) at 25°C while minimum (11mm) at 5°C in all varieties and moderate growth was observed at 15°C and 35°C. Golden delicious apple which have thin cuticle and prone to injuries more frequently as compared to other varieties was found most susceptible at all temperatures while Red delicious proved to be the most resistant against growth of pathogen at all temperature sets comparatively. It was concluded that Red delicious is most resistant against pathogen studied consequently can be saved for longer time without providing extraordinary conditions while Golden delicious apple should be kept in special environment to reduce damage of product after harvesting due to its most susceptibility against blue most disease.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Effect of incubation temperature on lesion diameter of Penicillium expansum o...Innspub Net
Current research work was conducted to determine impact of various genotypes and heat on shelf life of apple. Study was carried out in vitro. Effect of temperature on lesion diameter of fungal growth in most prominent apple fruit varieties growing in Pakistan was assessed. Four temperature (5, 15, 25 and 35°C) treatments were provided to fruits of each variety after inoculation with spore suspension adjusted at 106spores/ml and growth of P. expansum was measured for 12 days at 3 days interval. Pathogen showed maximum growth (23mm) at 25°C while minimum (11mm) at 5°C in all varieties and moderate growth was observed at 15°C and 35°C. Golden delicious apple which have thin cuticle and prone to injuries more frequently as compared to other varieties was found most susceptible at all temperatures while Red delicious proved to be the most resistant against growth of pathogen at all temperature sets comparatively. It was concluded that Red delicious is most resistant against pathogen studied consequently can be saved for longer time without providing extraordinary conditions while Golden delicious apple should be kept in special environment to reduce damage of product after harvesting due to its most susceptibility against blue most disease.
Biofumigation: A Potential Aspect for Suppression of Plant-Parasitic NematodesIJEABJ
Plant-parasitic nematode cause economic loss to crops throughout the world. Biofumigation is the environmental friendly control option for the suppression of plant-parasitic as well as other pathogenic soil microbes. Glucosinolates are the main active compound present in some plants which are responsible for biofumigation process. To increase the efficiency of biofumigation selection of varieties containing more glucosinolates is highly desirable. Plant growth stage, soil temperature, soil texture, moisture, soil depth and soil microbes play important role in efficient biofumigation.
Distribution, Biochemical Properties and Genetic Relatedness of Endophytic Ba...AI Publications
Microbe-assisted phytoremediation is a recent application of bioremediation with much prospects. The genetic relatedness of culturable endophytic bacteria of wetland plants growing on a six month-old and twelve month-old petroleum-contaminated sites, and an uncontaminated site in Bayelsa State of the Niger Delta Region, Nigeria were compared. Most of the endophyte species isolated from the roots, stems and leaves were common to all the sites and belong to the phyla Proteobacteria, Bacteroidetes Firmicutes, Actinobacteria, Chloroflexi and Actinomicrobia, with the γ-Proteobacteria dominating. Pseudomonas was the most prevalent species in all three sites, but higher in the petroleum contaminated sites. Biochemical properties (API 20E) of the common isolates; Pseudomonas spp.Chryseobacterium indologenes,Bacillus and Proteusvaried with sites while only Providencia rettgeri peculiar to the petroleum-contaminated sites showed the same properties. 16S rRNA PCR-DNA fragments of forty-five species of the isolates (15 from each site) were characterized using RFLP and MspI restriction enzyme and a genetic distance tree of the restriction patterns drawn. The percentage of similarity in the genetic relatedness of isolates ranged from 11.1 – 100%. The genetic tree analysis of the 45 species of identified bacteria revealed 3 major clusters with 17 DNA fingerprinting patterns. Pseudomonas species of the root and leaves of the six month-old petroleum-contaminated site and uncontaminated site were seen to cluster together irrespective of date of isolation. The endophytes may play a role in the in situ degradation of the petroleum hydrocarbon of the sites.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium...researchagriculture
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production.
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163.
Full Text:
http://www.jagri.info/documents/AG0040.pdf
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Alli...researchagriculture
Cabbage aphid (
Brevicoryne brassicae
L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of
Tephrosia vogelii
,
Allium sativum
and
Solanum incanum
in controlling
Brevicoryne brassicae
in
Brassica napus
production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were
T
.
vogelii
,
A
.
sativum
,
S
.
incanum
,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that
T. vogelii
,
S
.
incanum
and
A
.
sativum
aqueous crude extracts have some pesticidal
effects on aphid in rape
production.
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
In vitro evaluation of Trichoderma viride and Trichoderma harzianum for its e...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Abstract— This study was conducted to identify, test the pathogenicity of strawberry root and stalk rot pathogens and evaluate the efficiency of some biocontrol agents and fungicides to control the disease. The isolation and identification of fungi associated with infected plant samples showed that Rhizoctonia solani was detected in all studied commercial strawberry lath houses at different location of Baghdad-Iraq. The frequency percentages ranged 25.5-63.5 % and 10.75 - 40 % for Rhizoctonia solani and Phymatotrichopsis omnivora respectively. Pathogenicity test revealed R. solani and P. omnivora isolates were highly pathogenic to strawberry plants. The disease severity percentages of R. solani and P. omnivora were 83.0-100% and 55.5-62.0 % respectively. The isolates HRs3 and KPh1 of R. solani and P. omnivora respectively, caused the highest disease were used during this study. The control agents Rizolex and Tachigarin fungicides, Azotobacter chroococcum and Pseudomonas fluorescens have shown high efficiency against R. solani and P. omnivora on culture media (PDA).
The treatment of biocontrol agent’s A. chroococcum and P. fluorescens and the fungicide Rizolex and Preserve Pro showed high efficiency in disease control and enhance plants growth under greenhouse conditions. Disease severities on foliar and root system in A. chroococcum , Rizolex , Preserve Pro and P. fluorescens were 6,66 and 0.00 %, 20.00 and 0.00 %,13.33 and 0.00 % and 13.33and 0.00 % respectively in plants infected with R. solani .Whereas they were 6.66 and 0.00%, 13.33 and 0.00 %,13.33 and 0.00 %,and 13.33 and 0.00 % respectively in plants infected with P. omnivora. This study is the first report of the occurrence of root and stalk rot disease caused by R. solani and P. omnivora on strawberry plants in Iraq.
Nodulation, Growth and Yield Response of Five Cowpea (Vigna unguiculata L. Wa...Premier Publishers
The experiment was carried out in the screen house of the Department of Crop, Soil & Pest Management, Federal University of Technology, Akure, Nigeria. The experimental layout was a 5 x 3 x 2 factorial combination with 3 replications given a total of 90 treatments. Seeds of five cowpea varieties namely: IT98K-205-8, Ife Brown, Oloyin Brown, IT98K-573-2-1 and IT96D-610 were sown in Plastic buckets of 7-liter capacity and were perforated at the bottom to allow for drainage and filled with top soil. Watering regimes of (500ml, 700ml and 900ml) were imposed and water was applied twice a week while cowpea plants were inoculated with 5g each of Rhizobia strain (Mesorhizobia loti) at seedling stage. Control set was maintained without inoculation. The effect of watering regimes on legume species was significant on nodulation, growth and yield characters of cowpea varieties evaluated. The results revealed marked varietal differences in plant growth, nodulation, yield and yield components. IT98K-573-2-1 and Oloyin Brown generally expressed superior performance in most measured parameters. Mesorhizobia inoculation significantly (p≤0.05) increased plant growth, nodulation, yield and yield components of cowpea. The interaction effect of variety, Mesorhizobia loti and watering regimes caused significant variations in the number of nodules, leaf area, number of seeds/pod and seed yield. The nitrogen and crude protein content in the leaf differed among the cowpea varieties evaluated. Application of mesorhizobium strain significantly increased seed yield of cowpea and caused substantial increase in nodulation and this subsequently affected the Nitrogen fixation potential of cowpea under varying soil moisture regimes.
Glyphosate resistance trait into soybean Cuban varieties: agronomical assessm...Innspub Net
Glyphosate-resistant (GR) soybean was one of the first major applications of genetic engineering in field crops and offered farmers a vital tool in fighting weeds. Weeds are a problem for soybean production in Cuba, so our work aim was the GTS 40-3-2 event introgression into Cuban varieties. Two local cultivars were crossed with transgenic genotypes that carry the event. From F1 to F3 generations, individual plants that produced more than 60 g of seeds per plant were chosen to obtain next generation. Individual lines were selected from F4 generation. F5 and F6 generations of five selected transgenic lines and their relatives were chosen to evaluate seven
agronomic traits throughout the summers of 2012 and 2013. A Random Block experimental design was done. First flowering (R1) and maturity (R8) stages of all genotypes were affected by planting date. Plant height of I1B2- 3, I1B4, I36B4 and RP5 lines ranged from 80 to 111 cm. I1B2-2 and I1B2-3 lines would be suitable for mechanized harvesting because they had the insertion of the first pod at 14.63 cm and 13.93 cm respectively. I36B4 line produced the greatest number of pods per plant (127). Transgenic lines produced more than 180 seeds per plant and 100-seed weight ranged from 13.75 g to 17.46 g. Seed yield per plant of transgenic lines and their parents IncaSoy36, CEB2 and CEB4 weren’t statistically different. These results could be a start point for other studies
involving larger areas, different planting dates and localities. Get more articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
The objectives of this study were to detect and characterize the phytoplasma in tissues of diseased hibiscus plants using Dains’ stain light microscopy and molecular based techniques. Molecular characterization was performed using the DNA sequencing and phylogenetic analysis of the spacer region between 16S and 23S rRNA fragment of the isolated phytoplasma genome. This work concerning phytoplasma associated witches' broom (group 16SrII) diseases of hibiscus plants is achieved for the first time in Egypt.
Degradation of Industrial Fabric Dyes used in Sanganer Area by Endophytic Mic...ijtsrd
Dyes and dyestuffs find use in a wide range of industries but are of primary importance to textile manufacturing. Wastewater from the textile industry can contain a variety of polluting substances including dyes. Increasingly, environmental legislation is being imposed to control the release of dyes, in particular fabric based compounds, into the environment. The ability of microorganisms to decolourise and metabolise dyes has long been known, and the use of bioremediation based technologies for treating textile wastewater has attracted interest. Within this review, we investigate the mechanisms by which diverse categories of microorganisms, such as the white rot fungi and anaerobic bacterial consortia, bring about the degradation of dyestuffs. Rapid industrialization has given rise to various unwanted elements that accumulated in the biosphere up to toxic levels to degrade the natural environment. Scientific developments are considered as key factors for progress of both developing and under developed countries, but unfortunately, most of the industries in these countries do not have proper waste treatment facilities and releasing a large quantity of effluents. A majority of xenobiotics either untreated or partially treated released from industries are mixed up with the natural water bodies and to the soil of the biosphere. Untreated or partially treated textile effluents are highly toxic, as they contain a large number of toxic chemicals and heavy metals. The problem of water pollution due to the discharge of industrial wastewater into natural water bodies was witnessed by western countries in 19th century and also in India after independence Rekha Soni "Degradation of Industrial Fabric Dyes used in Sanganer Area by Endophytic Microbes" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38360.pdf Paper Url: https://www.ijtsrd.com/biological-science/botany/38360/degradation-of-industrial-fabric-dyes-used-in-sanganer-area-by-endophytic-microbes/rekha-soni
Biofumigation: A Potential Aspect for Suppression of Plant-Parasitic NematodesIJEABJ
Plant-parasitic nematode cause economic loss to crops throughout the world. Biofumigation is the environmental friendly control option for the suppression of plant-parasitic as well as other pathogenic soil microbes. Glucosinolates are the main active compound present in some plants which are responsible for biofumigation process. To increase the efficiency of biofumigation selection of varieties containing more glucosinolates is highly desirable. Plant growth stage, soil temperature, soil texture, moisture, soil depth and soil microbes play important role in efficient biofumigation.
Distribution, Biochemical Properties and Genetic Relatedness of Endophytic Ba...AI Publications
Microbe-assisted phytoremediation is a recent application of bioremediation with much prospects. The genetic relatedness of culturable endophytic bacteria of wetland plants growing on a six month-old and twelve month-old petroleum-contaminated sites, and an uncontaminated site in Bayelsa State of the Niger Delta Region, Nigeria were compared. Most of the endophyte species isolated from the roots, stems and leaves were common to all the sites and belong to the phyla Proteobacteria, Bacteroidetes Firmicutes, Actinobacteria, Chloroflexi and Actinomicrobia, with the γ-Proteobacteria dominating. Pseudomonas was the most prevalent species in all three sites, but higher in the petroleum contaminated sites. Biochemical properties (API 20E) of the common isolates; Pseudomonas spp.Chryseobacterium indologenes,Bacillus and Proteusvaried with sites while only Providencia rettgeri peculiar to the petroleum-contaminated sites showed the same properties. 16S rRNA PCR-DNA fragments of forty-five species of the isolates (15 from each site) were characterized using RFLP and MspI restriction enzyme and a genetic distance tree of the restriction patterns drawn. The percentage of similarity in the genetic relatedness of isolates ranged from 11.1 – 100%. The genetic tree analysis of the 45 species of identified bacteria revealed 3 major clusters with 17 DNA fingerprinting patterns. Pseudomonas species of the root and leaves of the six month-old petroleum-contaminated site and uncontaminated site were seen to cluster together irrespective of date of isolation. The endophytes may play a role in the in situ degradation of the petroleum hydrocarbon of the sites.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium...researchagriculture
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production.
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163.
Full Text:
http://www.jagri.info/documents/AG0040.pdf
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Alli...researchagriculture
Cabbage aphid (
Brevicoryne brassicae
L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of
Tephrosia vogelii
,
Allium sativum
and
Solanum incanum
in controlling
Brevicoryne brassicae
in
Brassica napus
production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were
T
.
vogelii
,
A
.
sativum
,
S
.
incanum
,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05)><0.05) on the yield of rape. It was concluded that
T. vogelii
,
S
.
incanum
and
A
.
sativum
aqueous crude extracts have some pesticidal
effects on aphid in rape
production.
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
In vitro evaluation of Trichoderma viride and Trichoderma harzianum for its e...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Abstract— This study was conducted to identify, test the pathogenicity of strawberry root and stalk rot pathogens and evaluate the efficiency of some biocontrol agents and fungicides to control the disease. The isolation and identification of fungi associated with infected plant samples showed that Rhizoctonia solani was detected in all studied commercial strawberry lath houses at different location of Baghdad-Iraq. The frequency percentages ranged 25.5-63.5 % and 10.75 - 40 % for Rhizoctonia solani and Phymatotrichopsis omnivora respectively. Pathogenicity test revealed R. solani and P. omnivora isolates were highly pathogenic to strawberry plants. The disease severity percentages of R. solani and P. omnivora were 83.0-100% and 55.5-62.0 % respectively. The isolates HRs3 and KPh1 of R. solani and P. omnivora respectively, caused the highest disease were used during this study. The control agents Rizolex and Tachigarin fungicides, Azotobacter chroococcum and Pseudomonas fluorescens have shown high efficiency against R. solani and P. omnivora on culture media (PDA).
The treatment of biocontrol agent’s A. chroococcum and P. fluorescens and the fungicide Rizolex and Preserve Pro showed high efficiency in disease control and enhance plants growth under greenhouse conditions. Disease severities on foliar and root system in A. chroococcum , Rizolex , Preserve Pro and P. fluorescens were 6,66 and 0.00 %, 20.00 and 0.00 %,13.33 and 0.00 % and 13.33and 0.00 % respectively in plants infected with R. solani .Whereas they were 6.66 and 0.00%, 13.33 and 0.00 %,13.33 and 0.00 %,and 13.33 and 0.00 % respectively in plants infected with P. omnivora. This study is the first report of the occurrence of root and stalk rot disease caused by R. solani and P. omnivora on strawberry plants in Iraq.
Nodulation, Growth and Yield Response of Five Cowpea (Vigna unguiculata L. Wa...Premier Publishers
The experiment was carried out in the screen house of the Department of Crop, Soil & Pest Management, Federal University of Technology, Akure, Nigeria. The experimental layout was a 5 x 3 x 2 factorial combination with 3 replications given a total of 90 treatments. Seeds of five cowpea varieties namely: IT98K-205-8, Ife Brown, Oloyin Brown, IT98K-573-2-1 and IT96D-610 were sown in Plastic buckets of 7-liter capacity and were perforated at the bottom to allow for drainage and filled with top soil. Watering regimes of (500ml, 700ml and 900ml) were imposed and water was applied twice a week while cowpea plants were inoculated with 5g each of Rhizobia strain (Mesorhizobia loti) at seedling stage. Control set was maintained without inoculation. The effect of watering regimes on legume species was significant on nodulation, growth and yield characters of cowpea varieties evaluated. The results revealed marked varietal differences in plant growth, nodulation, yield and yield components. IT98K-573-2-1 and Oloyin Brown generally expressed superior performance in most measured parameters. Mesorhizobia inoculation significantly (p≤0.05) increased plant growth, nodulation, yield and yield components of cowpea. The interaction effect of variety, Mesorhizobia loti and watering regimes caused significant variations in the number of nodules, leaf area, number of seeds/pod and seed yield. The nitrogen and crude protein content in the leaf differed among the cowpea varieties evaluated. Application of mesorhizobium strain significantly increased seed yield of cowpea and caused substantial increase in nodulation and this subsequently affected the Nitrogen fixation potential of cowpea under varying soil moisture regimes.
Glyphosate resistance trait into soybean Cuban varieties: agronomical assessm...Innspub Net
Glyphosate-resistant (GR) soybean was one of the first major applications of genetic engineering in field crops and offered farmers a vital tool in fighting weeds. Weeds are a problem for soybean production in Cuba, so our work aim was the GTS 40-3-2 event introgression into Cuban varieties. Two local cultivars were crossed with transgenic genotypes that carry the event. From F1 to F3 generations, individual plants that produced more than 60 g of seeds per plant were chosen to obtain next generation. Individual lines were selected from F4 generation. F5 and F6 generations of five selected transgenic lines and their relatives were chosen to evaluate seven
agronomic traits throughout the summers of 2012 and 2013. A Random Block experimental design was done. First flowering (R1) and maturity (R8) stages of all genotypes were affected by planting date. Plant height of I1B2- 3, I1B4, I36B4 and RP5 lines ranged from 80 to 111 cm. I1B2-2 and I1B2-3 lines would be suitable for mechanized harvesting because they had the insertion of the first pod at 14.63 cm and 13.93 cm respectively. I36B4 line produced the greatest number of pods per plant (127). Transgenic lines produced more than 180 seeds per plant and 100-seed weight ranged from 13.75 g to 17.46 g. Seed yield per plant of transgenic lines and their parents IncaSoy36, CEB2 and CEB4 weren’t statistically different. These results could be a start point for other studies
involving larger areas, different planting dates and localities. Get more articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
The objectives of this study were to detect and characterize the phytoplasma in tissues of diseased hibiscus plants using Dains’ stain light microscopy and molecular based techniques. Molecular characterization was performed using the DNA sequencing and phylogenetic analysis of the spacer region between 16S and 23S rRNA fragment of the isolated phytoplasma genome. This work concerning phytoplasma associated witches' broom (group 16SrII) diseases of hibiscus plants is achieved for the first time in Egypt.
Degradation of Industrial Fabric Dyes used in Sanganer Area by Endophytic Mic...ijtsrd
Dyes and dyestuffs find use in a wide range of industries but are of primary importance to textile manufacturing. Wastewater from the textile industry can contain a variety of polluting substances including dyes. Increasingly, environmental legislation is being imposed to control the release of dyes, in particular fabric based compounds, into the environment. The ability of microorganisms to decolourise and metabolise dyes has long been known, and the use of bioremediation based technologies for treating textile wastewater has attracted interest. Within this review, we investigate the mechanisms by which diverse categories of microorganisms, such as the white rot fungi and anaerobic bacterial consortia, bring about the degradation of dyestuffs. Rapid industrialization has given rise to various unwanted elements that accumulated in the biosphere up to toxic levels to degrade the natural environment. Scientific developments are considered as key factors for progress of both developing and under developed countries, but unfortunately, most of the industries in these countries do not have proper waste treatment facilities and releasing a large quantity of effluents. A majority of xenobiotics either untreated or partially treated released from industries are mixed up with the natural water bodies and to the soil of the biosphere. Untreated or partially treated textile effluents are highly toxic, as they contain a large number of toxic chemicals and heavy metals. The problem of water pollution due to the discharge of industrial wastewater into natural water bodies was witnessed by western countries in 19th century and also in India after independence Rekha Soni "Degradation of Industrial Fabric Dyes used in Sanganer Area by Endophytic Microbes" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38360.pdf Paper Url: https://www.ijtsrd.com/biological-science/botany/38360/degradation-of-industrial-fabric-dyes-used-in-sanganer-area-by-endophytic-microbes/rekha-soni
4 ijfaf nov-2017-2-allelopathic effect of eucalyptusAI Publications
Laboratory and greenhouse experiments were carried out at the Faculty of Agricultural Sciences, University of Gezira, Sudan in season 2014/15 to study the allelopathic effects of Eucalyptus (Eucalyptus camaldulensis Dehnh) leaf on seed germination and seedling growth of some poaceous crops. Laboratory experiments were conducted to study the allelopathic effects of leaf aqueous extract of Eucalyptus on seed germination of sorghum (Sorghum bicolor [L.] Moench), millet (Pennisetum glaucum [L.] R. Br.), maize (Zea mays L.) and wheat (Triticum vulgare L.). Six concentrations (0, 20, 40, 60, 80 and 100%) of the leaf aqueous extract of Eucalyptus were prepared from the stock solution (50 g / l). Treatments, for each crop, were arranged in completely randomized design with four replicates. The seeds were examined for germination at three days after initial germination. Greenhouse experiments were conducted to study the allelopathic effects of Eucalyptus leaf powder on seedling growth of the same crops. The leaf powder of Eucalyptus was incorporated into the soil at rate of 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% on w/w bases in pots. Treatments, for each crop, were arranged in completely randomized design with four replicates. The experiments were terminated at 30 days after sowing and the plant height, number of leaves and root length of crop seedlings were measured as well as plant fresh and dry weight. Data were subjected to analysis of variance procedure. Means were separated for significance using Duncan`s Multiple Range Test at p 0.5. The results showed that the leaf aqueous extract of Eucalyptus significantly reduced seed germination of the tested poaceous crops and there was direct negative relationship between concentration and germination. Also, the results showed that incorporating leaf powder of Eucalyptus into the soil significantly decreased plant height, number of leaves and root length of crop seedlings as well as seedling fresh and dry weight. In addition, the reduction in seedling growth was increased as the leaf powder increased in the soil. Based on results supported by different studies, it was concluded that Eucalyptus has allelopathic effects on seed germination and seedling growth of the poaceous crops.
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Two field experiment were run at the farms of Omar AL-Mokhtar university, in order to knew the effect of some methods used to control tomato pests on soil arthropod systemic groups in organic cultivated tomato field such as (BT, algifol, methyl salicylate, neem oil), Results indicated that application of B. thuringensis and Algifol achieved the highest performance showing 100% reduction in mites and other soil arthropods systemic groups, and gave reduction 55.5 and 54.96% in both insect and total population of soil arthropods collected with pitfall traps. As far as Neemazone and Methyl salicylate increased the insect population with percentages 132.5 and 310% and total soil arthropods with 133.2% and 308.6% respectively and reduced the mites and the other arthropods population with 100%. Also results showed that the pesticides (avermectin, indoxacarb, & neemazone) increased the average number of the total soil arthropod population groups, and these pesticides showed selective effect, so that, it will be recommended that in order to preserve the beneficial predators, carnivorous and parasitoids these biological and selective pesticides in conventional tomato field should be applied.
According to the results obtained from this work, the changes in diversity and equitability and decrease or increase of percent population of soil arthropods groups were differed according to the soil arthropod groups, sampling period, applied plant protection products and system of agricultural.
Resistance/tolerance to root-knot nematode Meloidogyne incognita (Kofoid and ...Premier Publishers
This experiment was carried out in a Randomized Complete Block Design (40 X 10m plot) to evaluate cacao clones for resistance and tolerance against Meloidogyne incognita in infested field. The ten cocoa clones evaluated are MXC67, T86/2, PA150, LCTEEN, T12/11, T53/5, T101/15, T65/7, ICS1 and AMAZ 15-15. Pre-planting survey revealed ten genera of phytonematodes, these were; Meloidogyne spp., Pratylenchus spp., Helicotylenchus spp. Paralongidorus spp., Eutylenchus spp., Scutellonema spp., Hemicyclophora spp., Xiphinema spp., Longidorus spp., Anguillulina spp., Psilenchus spp., and Tetylenchus spp. Meloidogyne spp. had the highest frequency of occurrence and highest population in 250g soil (40 and 28,234 respectively), Paralongidorus spp. was next in population. Based on gall index, nematode reproduction factor and growth parameters, MXC67, T86/2, PA150, T101/15 and T53/5 were susceptible to the nematode. Two other clones, T65/7 and ICS1 were tolerant. A high degree of resistance was exhibited by LCTEEN, T12/11 and AMAZ 15-15. Compared with F3 Amazon and Amelonado varieties, the two most famous cocoa varieties in Nigeria, four clones (LCTEEN, T65/7, ICSI and AMAZ 15-15) were superior to F3 Amazon and Amelonado. This study showed that planting of resistance/tolerant cacao clones in nematode infested soil will drastically reduce seedling failure experienced by farmers. This tolerant and resistant clones identified should be included in breeding programme for resistance.
Micro-propagation of Alstroemeria Hybrida Cv. PlutoIJEAB
The experiment entitled micropropagation of Alstroemeria hybrida cv. Pluto was conducted to standardize protocol for aseptic establishment, callus induction, proliferation, and rooting from rhizome tips, rhizome sections, shoot tips, shoot nodal segments and inflorescence buds. Highest culture asepsis of 79.20 per cent at 2 weeks of culture and 68.08 per cent at 4 weeks of culture was recorded in rhizome tips following sterilization treatment with Carbendazim 200 ppm for 30 minutes + HgCl2 (0.1 %) dip for 10 minutes and final treatment with ethyl alcohol (70 %) for 1 minute. Rhizome tips and rhizome section explants survived sterilant treatment better than other explants. MS-liquid medium supplemented with BAP + IBA: 1.5 + 0.2 mg l-l proved best for culture establishment (89.42 %) in case of rhizome tips and (56.13 %) in case of rhizome sections. MS-solid medium with plant growth regulator combinations BAP + IBA: 1.0 + 0.2 mg l-1 fortified with activated charcoal resulted in an establishment of (78.25 %) in rhizome tips and (40.24 %) in case of rhizome sections. Callus induction was highest in MS-solid medium fortified with BAP + NAA: 0.5 + 4.5 mg l-l. Rhizome tips cultured on MS-medium BAP + IBA + GA3 + Activated charcoal: 2.0 + 0.4 + 0.5 + 1000 mg l-l gave highest proliferation (88.85 %) along with highest number of erect shoots (5.75) , number of new rhizome buds ( 3.75), rhizome fresh weight/shoot complex (6.05), and multiplication index (2.76). Highest Rooting (54.81 %) along with lowest days to appearance of root (10.87), highest number of roots (3.12) and highest root length (16.42 mm) was recorded in MS-liquid medium fortified with NAA 1.5 mg l-1. Abbreviations used— AC; Activated charcoal, BAP; 6-Benzyl amino purine, BA; 6-Benzyladenine, 2, 4-D; 2, 4dichloro-phenoxyacetic acid,GA3; Gibberelic acid, IAA; Indole-3-acetic acid, IBA; Indole-3-butyric acid, MS; Murashige and Skoog’s (1962) medium, NAA; Naphthalene acetic acid and µm; Micro molar.
Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yi...IJEAB
Trichoderma species are commonly used as effective biological control agents against phytopathogens especially the soil-borne fungi while some isolates are able to ameliorate plant growth. In the present study, Trichoderma fortified compost with different substrates were evaluated to reduce the pre-emergence and post-emergence seedling mortality, diseases of stem and root of chickpea caused by several soil-borne fungal pathogens, including Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii at different growth stages in the field under natural epiphytotic conditions. Among the twenty isolates of T. harzianum, Co-7 showed the most effective antagonist against the test pathogens in dual culture method. In field experiment, subsequently it was used for inoculum preparation with colonized wheat grain and mixed with well-matured decomposed composting materials like, saw dust, cow dung, tea waste, water hyacinth and poultry manure. Trichoderma fortified compost with poultry manure was found significantly effective in reducing pre-emergence and post emergence seedling mortality, disease incidence and disease severity of chickpea in the field. Interestingly, all the treatments significantly increased but Trichoderma fortified compost with poultry manure was the best to boost seed yield and quality.
This survey was conducted on an experimental parcel of the Agronomic High School of the National Polytechnic Institute Félix HOUPHOUET- BOIGNY (ESA-INPHB) of Yamoussoukro (Côte d’Ivoire). It aims to evaluate the insecticidal effect of 2 local plants aqueous extracts on termites (Azadirachta indica and Cassia siamea) dosed at 131.25 g powder/ L compared to a chemical pesticide (Chlorpyrifos ethyl) dosed at 480 g / L. A total of 11 termite species were collected from the plots. The fungus-growers with 6 species are the most dominant group. Four soil-feeder species and one wood-boring species were also collected. Only fungus-growers are present in the areas treated with plant extracts and chlorpyrifos ethyl. It was observed a high abundance of termites in heading stage of the rice growth. In maturity stage, ie 4 months after sowing the efficiency of chlorpyrifos ethyl decreased as plant aqueous extracts remain effective. Among the two tested plants, Azadirachta indica seems to be the most effective compared to Cassia siamea against termites. Chlorpyrifos ethyl could be used as a cure for its swift action on termites while Azadirachta indica could be used preventively and for crops whose destructive action is long term.
effect of an endomycorrhizal inoculum on the growth of argan tree plantsIJEAB
The aim of this work is to study the effect of a composite endomycorrhizal inoculum on the growth of argan tree plants under nursery conditions. Analysis of the obtained results after ten months of inoculation showed a significant effect on the growth of the inoculated plants as compared to the controls. Indeed, the mean values of arial fresh weight (27.54 g) and root (23.64 g). The length (59.87 cm), the collar diameter (3.93 cm) and the number of branches (7.37) of the inoculated plants are superior to those observed in the control plants, 13.36 g, 13.43 g, 35.83 cm, 2.83 cm and 4.66 cm, respectively. In addition, frequency (100%), intensity (63.66%) and arbuscule contents (51.79%) and vesicles (25.52%) are very important. The roots of the control plants are not mycorrhizal. The mean number of spores formed in the rhizosphere of the inoculated plants is 246 spores per 100 g of soil. These spores are those of 29 endomycorrhizal species belonging to six different genera: Acaulospora, Scutllospora, Pacispora, Glomus, Entrophospora and Gigaspora. Representatives of the Glomus genus are the most dominant.
Similar to Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahliae Kleb. on Olive Tree under Greenhouse Conditions (20)
Evaluation of Agro-morphological Performances of Hybrid Varieties of Chili Pe...Premier Publishers
In Benin, chilli pepper is a widely consumed as vegetable whose production requires the use of performant varieties. This work assessed, at Parakou and Malanville, the performance of six F1 hybrids of chilli including five imported (Laali, Laser, Nandi, Kranti, Nandita) and one local (De cayenne), in completely randomized block design at four replications and 15 plants per elementary plot. Agro-morphological data were collected and submitted to analysis of variance and factor analysis of mixed data. The results showed the effects of variety, location and their interactions were highly significant for most of the growth, earliness and yield traits. Imported hybrid varieties showed the best performances compared to the local one. Multivariate analysis revealed that 'De cayenne' was earlier, short in size, thin-stemmed, red fruits and less yielding (≈ 1 t.ha-1). The imported hybrids LaaliF1 and KrantiF1 were of strong vegetative vigor, more yielding (> 6 t.ha-1) by developing larger, long and hard fruits. Other hybrids showed intermediate performances. This study highlighted the importance of imported hybrids in improving yield and preservation of chili fruits. However, stability and adaptation analyses to local conditions are necessary for their adoption.
An Empirical Approach for the Variation in Capital Market Price Changes Premier Publishers
The chances of an investor in the stock market depends mainly on some certain decisions in respect to equilibrium prices, which is the condition of a system competing favorably and effectively. This paper considered a stochastic model which was latter transformed to non-linear ordinary differential equation where stock volatility was used as a key parameter. The analytical solution was obtained which determined the equilibrium prices. A theorem was developed and proved to show that the proposed mathematical model follows a normal distribution since it has a symmetric property. Finally, graphical results were presented and the effects of the relevant parameters were discussed.
Influence of Nitrogen and Spacing on Growth and Yield of Chia (Salvia hispani...Premier Publishers
Chia is an emerging cash crop in Kenya and its production is inhibited by lack of agronomic management information. A field experiment was conducted in February-June and May-August 2021, to determine the influence of nitrogen and spacing on growth and yield of Chia. A randomized complete block design with a split plot arrangement was used with four nitrogen rates as the main plots (0, 40, 80, 120 kg N ha-1) and three spacing (30 cm x 15 cm (s1), 30 cm x 30 cm (s2), 50 cm x 50 cm (s3)). Application of 120 kg N ha-1 significantly increased (p≤0.05) vegetative growth and seed yield of Chia. Stem height, branches, stem diameter and leaves increased by 23-28%, 11-13%, 43-55% and 59-88% respectively. Spacing s3 significantly increased (p≤0.05) vegetative growth. An increase of 27-74%, 36-45% and 73-107% was recorded in number of leaves, stem diameter and dry weight, respectively. Chia yield per plant was significantly higher (p≤0.05) in s3. However, when expressed per unit area, s1 significantly produced higher yields. The study recommends 120 kg N ha-1 or higher nitrogen rates and a closer spacing of 15 cm x 30 cm as the best option for Chia production in Kenya.
Enhancing Social Capital During the Pandemic: A Case of the Rural Women in Bu...Premier Publishers
Social capital plays an essential role in empowering people for social and economic change even during the pandemic. A livelihood project of the government was implemented among the members of a women’s association of a disadvantaged upland community in Bukidnon province, Southern Philippines for inclusive development. This study was conducted to determine the influence of some socio-economic attributes and the change in the knowledge level on the social capital of the rural women amidst the pandemic. The activities of the project were implemented considering the health protocols imposed by the government during the health crisis. The findings revealed that the trainings conducted resulted to a positive change in the knowledge level among the rural women. This facilitated the production of vegetables for their households and generated additional income very necessary during the pandemic especially that other economic activities were hindered. Similarly, there was a significant increase in the social capital of the rural women during the last two years. The main occupation, sources of income and their ethnicity significantly influenced the social capital of the rural women. The rural development workers and policymakers must consider the social capital of the group in the implementation of poverty alleviation programs.
Impact of Provision of Litigation Supports through Forensic Investigations on...Premier Publishers
This paper presents an argument through the fraud triangle theory that the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases is adequate for effective prosecution of perpetrators as well as corporate fraud prevention. To support this argument, this study operationalized provision of litigation supports through forensic audit and investigations, data mining for trends and patterns, and fraud data collection and preparation. A sample of 500 respondents was drawn from the population of professional accountants and legal practitioners in Nigeria. Questionnaire was used as the instrument for data collection and this was mailed to the respective respondents. Resulting responses were analyzed using the OLS multiple regression techniques via the SPSS statistical software. The results reveal that the provision of litigation supports through forensic audits and investigations, fraud data mining for trends and patterns and fraud data collection and preparation for court proceedings have a positive and significant impact on corporate fraud prevention in Nigeria. This study therefore recommends that regulators should promote the provision of litigation supports through forensic audits and investigations in relation to corporate fraud cases in publicly listed firms in Nigeria, as this will help provide reports that are acceptable in court proceedings.
Improving the Efficiency of Ratio Estimators by Calibration WeightingsPremier Publishers
It is observed that the performances of most improved ratio estimators depend on some optimality conditions that need to be satisfied to guarantee better estimator. This paper develops a new approach to ratio estimation that produces a more efficient class of ratio estimators that do not depend on any optimality conditions for optimum performance using calibration weightings. The relative performances of the proposed calibration ratio estimators are compared with a corresponding global [Generalized Regression (GREG)] estimator. Results of analysis showed that the proposed calibration ratio estimators are substantially superior to the traditional GREG-estimator with relatively small bias, mean square error, average length of confidence interval and coverage probability. In general, the proposed calibration ratio estimators are more efficient than all existing estimators considered in the study.
Urban Liveability in the Context of Sustainable Development: A Perspective fr...Premier Publishers
Urbanization and quality of urban life are mutually related and however it varies geographically and regionally. With unprecedented growth of urban centres, challenge against urban development is more in terms of how to enhance quality of urban life and liveability. Making sense of and measuring urban liveability of urban places has become a crucial step in the context of sustainable development paradigm. Geographical regions depict variations in nature of urban development and consequently level of urban liveability. The coastal regain of West Bengal faces unusual challenges caused by increasing urbanization, uncontrolled growth, and expansion of economic activities like tourism and changing environmental quality. The present study offers a perspective on urban liveability of urban places located in coastal region comprising of Purba Medinipur and South 24 Parganas districts. The study uses the liveability standards covering four major pillars- institutional, social, economic and physical and their indicators. This leads to develop a City Liveability Index to rank urban places of the region, higher the index values better the urban liveability. The data for the purpose is collected from various secondary sources. Study finds that the eastern coastal region of the country covering state of West Bengal depicts variations in index of liveability determined by physical, economic, social and institutional indicators.
Transcript Level of Genes Involved in “Rebaudioside A” Biosynthesis Pathway u...Premier Publishers
Stevia rebaudiana Bertoni is a plant which has recently been used widely as a sweetener. This medicinal plant has some components such as diterpenoid glycosides called steviol glycosides [SGs]. Rebaudioside A is a diterpenoid steviol glycoside which is 300 times sweeter than table sugar. This study was done to investigate the effect of GA3 (50 mg/L) on the expression of 14 genes involved in Rebaudioside A biosynthesis pathway in Stevia rebaudiana under in vitro conditions. The expression of DXS remarkably decreased by day 3. Also, probably because of the negative feedback of GA3 on MEP-drived isoprenes, GGDS transcript level reached its lowest amount after GA3 treatment. The abundance of DXR, CMS, CMK, MCS, and CDPS transcripts showed a significant increase at various days after this treatment. A significant drop in the expression levels of KS and UGT85C2 is detected during the first day. However, expression changes of HDR and KD were not remarkable. Results revealed that the level of transcript of UGT74G1 and UGT76G1 up regulated significantly 4 and 2 times higher than control, respectively. However, more research needs to shed more light on the mechanism of GA3 on gene expression of MEP pathway.
Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Mor...Premier Publishers
Information on genetic variability for biochemical characters is a prerequisite for improvement of tea quality. Thirteen introduced tea clones characterized with objective; assessing tea clones based on morphological characters at Melko and Gera research stations. The study was conducted during 2017/18 cropping season on experimental plots in RCBD with three replications. Data recorded on morphological traits like days from pruning to harvest, height to first branch, stem diameter, leaf serration density, leaf length, leaf width, leaf size, petiole length, leaf ratio, internode length, shoot length, number of shoot, canopy diameter, hundred shoot weight, fresh leaf yield per tree. Cluster analysis of morphological trait grouped into four clusters indicated, the existence of divergence among the tested clones. The maximum inter-cluster distance was between clusters I and IV (35.27) while the minimum inter cluster distance was observed between clusters I and II (7.8).Principal components analysis showed that the first five principal components with eigenvalues greater than one accounted 86.45% for 15 morphological traits. Generally, the study indicated presence of variability for several morphological traits. However, high morphological variation between clones is not a guarantee for a high genetic variation; therefore, molecular studies need to be considered as complementary to biochemical studies.
Causes, Consequences and Remedies of Juvenile Delinquency in the Context of S...Premier Publishers
This research work was designed to examine nature of juvenile offences committed by juveniles, causes of juvenile delinquency, consequences of juvenile delinquency and remedies for juvenile delinquency in the context of Sub-Saharan Africa with specific reference to Eritrea. Left unchecked, juvenile delinquents on the streets engage in petty theft, take alcohol or drugs, rape women, rob people at night involve themselves in criminal gangs and threaten the public at night. To shed light on the problem of juvenile delinquency in the Sub-Saharan region data was collected through primary and secondary sources. A sample size of 70 juvenile delinquents was selected from among 112 juvenile delinquents in remand at the Asmara Juvenile Rehabilitation Center in the Eritrean capital. The study was carried out through coded self-administered questionnaires administered to a sample of 70 juvenile delinquents. The survey evidence indicates that the majority of the juvenile respondents come either from families constructed by unmarried couples or separated or divorced parents where largely the father is missing in the home or dead. The findings also indicate that children born out of wedlock, families led by single mothers, lack of fatherly role models, poor parental-child relationships and negative peer group influence as dominant causes of juvenile infractions. The implication is that broken and stressed families are highly likely to be the breeding grounds for juvenile delinquency. The survey evidence indicates that stealing, truancy or absenteeism from school, rowdy or unruly behavior at school, free-riding in public transportation, damaging the book of fellow students and beating other young persons are the most common forms of juvenile offenses. It is therefore, recommended that parents and guardians should exercise proper parental supervision and give adequate care to transmit positive societal values to children. In addition, the government, the police, prosecution and courts, non-government organizations, parents, teachers, religious leaders, education administrators and other stakeholders should develop a child justice system that strives to prevent children from entering deeper into the criminal justice process.
The Knowledge of and Attitude to and Beliefs about Causes and Treatments of M...Premier Publishers
Stigma and discrimination associated with mental illness are a common occurrence in the Sub-Saharan region including Eritrea. Numerous studies from Sub-Saharan Africa suggest that stigma and discrimination are major problems in the community, with negative attitudes and behavior towards people with mental illness being widespread. In order to assess the whether such negative attitudes persist in the context of Eritrea this study explored the knowledge and perceptions of 90 Eritrean university students at the College of Business and Economics, the University of Asmara regarding the causes and remedies of mental illness A qualitative method involving coded self-administered questionnaires administered to a sample of 90 university students to collecting data at the end of 2019. The survey evidence points that almost 50% of the respondents had contact with a mentally ill person suggesting that the significant number of the respondents experienced a first-hand encounter and knowledge of mental illness in their family and community. The findings show an overall greater science-based understanding of the causes of mental illness to be followed by recommended psychiatric treatments. The survey evidence indicates that the top three leading causes of mental illness in the context of Eritrea according to the respondents are brain disease (76%), bad events in the life of the mentally ill person (66%) and substance abuse or alcohol taking, smoking, taking drugs like hashish. (54%). The majority of the respondents have a very sympathetic and positive outlook towards mentally ill persons suggesting that mentally illness does not simply affect a chosen individual rather it can happen to anybody regardless of economic class, social status, ethnicity race and religion. Medical interventions cited by the majority of the respondents as being effective treatments for mental illness centered on the idea that hospitals and clinics for treatment and even cures for psychiatric disease. Changing perceptions of mental illnesses in Eritrea that paralleled the very caring and sympathetic attitudes of the sample university students would require raising public awareness regarding mental illness through education, using the mass media to raise public awareness, integrating mental health into the primary health care system, decentralizing mental health care services to increase access to treatment and providing affordable service to maintain positive treatment outcomes.
Effect of Phosphorus and Zinc on the Growth, Nodulation and Yield of Soybean ...Premier Publishers
An investigation was carried out at Kogi State University Student Research and Demonstration farm Anyigba during the 2019 wet season to observe the effect of phosphorus and zinc on the growth, nodulation and yield of soybean. The treatments comprised three levels: phosphorus and zinc (0, 30 and 60 kg P2O5/ha; 0, 5 and 10kg Zn/ha) and two varieties TGX 536 – 02D and Samsoy 2. The investigation revealed that application of phosphorus affected growth, nodulation, yield and some yield components of soybean while zinc application, apart from the plant height, which is reduced significantly, had no significant effect on other growth characters, nodulation, yield and yield components. However, it was generally found to decrease most of the characters. Application of 60 kg P2O5/ha gave the highest growth and yield, while 30 kg P2O5/ha gave the highest nodulation. Application of 60 kg P2O5/ha significantly increased yield to 1.9t/ha, which was significantly higher over the control plots, which gave 1.7t/ha. Crude protein and oil contents of the seeds were not significantly affected by phosphorus application but were significantly affected by zinc application, which significantly decreased protein content as its amount an increase from 0 to 10 kg/ha, and significantly increased oil content from 0 to 5kg/ha and decreased it below 5kg/ha. It was also revealed that the two varieties responded similarly to phosphorus and zinc in terms of growth, grain yield and crude protein content of the seeds.
Influence of Harvest Stage on Yield and Yield Components of Orange Fleshed Sw...Premier Publishers
A field experiment was conducted at Adami Tullu Agricultural Research Center in 2018 under rainfed condition with supplementary irrigation to determine the influence of harvest stage on vine yield and tuberous root yield of orange fleshed sweet potato varieties. The experiment consisted of four harvest stages (105, 120, 135 and 150 days after planting) and Kulfo, Tulla and Guntute varieties. A 4 X 3 factorial experiment arranged in randomized complete block design with three replications was used. Interaction of harvest stage and variety significantly influenced above ground fresh biomass, vine length, marketable tuberous root weight per hectare, commercial harvest index and harvest index. The highest mean values of above ground fresh biomass (66.12 t/ha) and marketable tuberous root weight (56.39 t/ha) were produced by Guntute variety harvested at 135 days after planting. Based on the results, it can be recommended that, farmers of the study area can grow Guntute variety by harvesting at 135 days after planting to obtain optimum vine and tuberous root yields.
Performance evaluation of upland rice (Oryza sativa L.) and variability study...Premier Publishers
This study aimed at assessing genetic variability and to evaluate the performance of 13 improved upland rice varieties for yield and its components based on morphological traits. The field experiment was conducted using a randomized block design at Guraferda and Gimbo districts in the 2019 main cropping season. The analysis of variance (ANOVA) over the two locations revealed significant differences (p≤ 0.05) among varieties for days to 50% heading, days to 85% maturity, panicle length, thousand-grain weight, and grain yield. Similarly, the ANOVA for variety by location interactions depicted significant differences among the tested varieties for days to 50% heading, days to 85% maturity, and thousand-grain weight. High heritability was obtained from days to heading (88.5%), panicle length (85.0%), and grain yield (85.2%), which indicates these traits can be easily improved through selection. High to medium broad sense heritability and genetic advance as percentage of the mean for days to heading, thousand-grain weight, and grain yield indicates a good opportunity for improvement through selection using their phenotypic performance. This is mainly due to the high role of additive gene action in the expression of such traits. This study confirmed the presence of variability among varieties for most of the studied traits, which will create an opportunity for breeders to improve rice yield and other attributes.
Response of Hot Pepper (Capsicum Annuum L.) to Deficit Irrigation in Bennatse...Premier Publishers
This study was conducted at Enchete kebele in Benna-Tsemay Woreda, South Omo Zone to evaluate the response of hot pepper to deficit irrigation on yield and water productivity under furrow irrigation system. The experiment comprised four treatments (100 % of ETc, 85% of ETc, 70 % of ETc and 50% of ETc), respectively. The experiment was laid out in RCBD and replicated four times. The two years combined yield results indicated that, the maximum total yield (20.38 t/ha) was obtained from 100% ETc while minimum yield (12.92 t/ha) was obtained from 50% of ETc deficit irrigation level. The highest WUE 5.22 kg/ha mm-1 was obtained from 50% of ETc. Treatment of 100% ETc irrigation application had highest benefit cost ratio (4.5) than all others treatments. Applying 50% of ETc reduce the yield by 37% when compared to 100 % ETc. Accordingly, to achieve maximum hot pepper yield in areas where water is not scarce, applying 100% ETc irrigation water application level throughout whole growing season under furrow irrigation system is recommended. But, in the study area water scarcity is the major limiting factor for crop production. So, it is possible to get better yield and water productivity of hot pepper when we apply 85% ETc irrigation water throughout growing season under furrow irrigation system.
Harnessing the Power of Agricultural Waste: A Study of Sabo Market, Ikorodu, ...Premier Publishers
Nigeria is still burdened with huge responsibilities of waste disposal because the potential for benefits of proper waste management is yet to be harnessed. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. It is estimated that about 998 million tons of agricultural waste is produced yearly in the country with organic wastes amounting to 80 percent of the total solid wastes. This can be categorized into biodegradable and non-biodegradable wastes. The paper evaluates the capacity of the Sabo Cattle market in producing the required quantities of waste from animal dung alongside decomposed fruits with a view to generating renewable energy possibilities for lighting, security and other business activities of the market. The Sabo market was treated as a study case with the adoption of in-depth examinations of the facility, animals and products for sale and waste generated. A combination of experimental, interviews (qualitative) and design simulation (for final phase) was adopted to extract, verify and analyse the data generated from the study. Animal waste samples were subjected to compositional and fibre analysis with results showing that the sample has high potency for biogas production. Biodegradable Wastes are human and animal excreta, agricultural and all degradable wastes. Availability of high quantity of waste generated being organic in Sabo market allows the use of anaerobic digestion to be proposed as a waste to energy technology due to its feasibility for conversion of moist biodegradable wastes into biogas. The study found that at peak supply period during the Islamic festivities, a conservative 300tonnes of animal waste is generated during the week which translates to over 800kilowatts of electricity.
Influence of Conferences and Job Rotation on Job Productivity of Library Staf...Premier Publishers
The general purpose of this study is to investigate the influence of conferences and job rotation on job productivity of library staff in tertiary institutions in Imo State, Nigeria. The survey research design was used for this study using questionnaire as an instrument for data collection. This study covered the entire population of 661. Out of these, 501 copies of the questionnaire representing 75.8% were duly completed and returned for analysis. Student’s t-test was used to analyze the research questions. The finding showed that conferences had no significant influence on the job productivity of library staff in tertiary institutions in Imo State, Nigeria (F cal= 7.86; t-vale =6.177; p >0.005). Finding also showed that job rotation significantly influences job productivity of library staff in tertiary institutions in Imo State, Nigeria (F-cal value= 18.65; t-value = 16.225; P<0.05). This study recommended that, government should ensure that library staff participate in conferences with themes and topics that are relevant to the job they perform and also ensure that there should be proper evaluation and feedback mechanism which aimed to ensuring control and minimize abuse of their development opportunities. Again, there should be written statement of objectives in order to sustain job rotation programmes. Also, that training and development needs of library staff must be identified and analyzed before embarking on job rotation processes as this would help to build skills, competences, specialization and high job productivity.
Scanning Electron Microscopic Structure and Composition of Urinary Calculi of...Premier Publishers
Microscopic examination of urine samples collected from geriatric dogs revealed increased numbers of erythrocytes, leucocytes, epithelial cells and pus cells along with casts, bacteria, spermatozoa and crystals of various shapes. Among the different crystals, triple phosphate or struvite were predominant, followed by calcium oxalate dihydrate, calcium oxalate monohydrate and ammonium urate or biurate. The struvite crystals were, coffin-lid shape and while calcium oxalate dihydrate were octahedron or envelope and monohydrate crystals demonstrated “picket fence” and “dumbbell” and “hemp seed” appearance. Brown or yellow-brown spherical bodies with irregular borders with thorn-apple appearance were shown by ammonium urate or biurate crystals. SEM aspects of magnesium ammonium phosphate crystals revealed perpendicular columnar strata, few with scattered hexa or octa-hedral coffin-lid shaped crystals and calcium phosphate crystals were like cracked eggshells. Presence of wavy phases with sundry areas (uric acid), picket fence (calcium oxalate monohydrate) and typical envelope (calcium oxalate dehydrate) were electron microscopic appearance of various crystals.
Gentrification and its Effects on Minority Communities – A Comparative Case S...Premier Publishers
This paper does a comparative analysis of four global cities and their minority districts which have been experiencing the same structural pressure of gentrification. The main contribution of this paper is providing a detailed comparison of four micro geographies worldwide and the impacts of gentrification on them: Barrio Logan in San Diego, Bo-Kaap in Cape Town, the Mission District in San Francisco, and the Rudolfsheim-Fünfhaus District in Vienna. All four cities have been experiencing the displacement of minority communities due to increases in property values. These cities were chosen because their governments enacted different policies to temper the gentrification process. It was found that cities which implemented social housing and cultural inclusionary policies were more successful in maintaining the cultural and demographic make-up of the districts.
Oil and Fatty Acid Composition Analysis of Ethiopian Mustard (Brasicacarinata...Premier Publishers
The experiments was conducted at Holetta Agricultural Research Center, to analyze forty nine Ethiopian Mustard land races for oil and fatty acid composition traits The experiment was carried out in a simple lattice design. The analysis of variance showed that there were highly significant differences among genotypes for all oil and fatty acid traits compared. The significant difference indicates the existence of genetic variability among the land races which is important for improvement
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
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Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
2. Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahliae Kleb. on Olive Tree under Greenhouse Conditions
Khrieba et al. 186
2002; Jiménez‐Fernández et al., 2016). Diseases caused
by soil borne fungi can be influenced by the formation of
AMF in the root system (Artursson et al., 2006). In general,
mycorrhizal plants suffer less damage and the incidence
of deceased or the pathogen development is inhibited
(Dehne, 1982). Garmendia et al. (2006) suggested that the
arbuscular mycorrhizal fungus (AMF) Glomus deserticola
could reduce the deleterious effect of V. dahliae on pepper
growth and yield. In olive trees, Glomus intraradices and
G. claroideum have been shown to be the most influential
AM fungi (Caravaca et al., 2003). G. mosseae has been
successfully used in the mycorrhization of other woody
species such as Pinus halepensis and P. nigra (Pirazzi et
al., 1999; Monticelli et al., 2000). Olive trees are known to
form arbuscular mycorrhiza and get benefit of this
interaction (Roldan-Fajardo and Baerea, 1985). The most
common mycorrhizal type involved in normal cropping
systems, being considered as key component in
environmentally agro-biotechnologies (Jeffries and Bera,
2001). AMF fungi are known to play a critical role in plant
nutrition and enhancing mineral acquisition by the plant
(Smith and Read, 1997). One critical step for applying AMF
fungi is the appropriate selection. For this purpose, it is
recommended to test native ecotype that is considered
highly effective from established culture collections (Dodd
and Thomson, 1994). Considering the novel aspects in
studying rhizosphere ecology, a series of experiment was
carried out aimed at analyzing the natural diversity of AMF
in the rhizosphere of mature olive tree growing under
normal cultivation, at determining the efficiency of AMF
fungi isolated from target olive plantation as inoculants for
the target olive cultivars. The aim of the present study was
to examine the influence of AMF fungi on the growth and
resistance to V. dahliae.
MATERIALS AND METHODS
Sampling of olive soils
Soil samples from mature field-grown olive trees were
collected in a major olive plantation area in the Guilan
province (Rudbar, loshan and Manjil), in northern Iran.
Gilan has a humid subtropical climate with, by a large
margin, the heaviest rainfall in Iran: reaching as high as
1,900 millimetres (75 in) in the southwestern coast and
generally around 1,400 millimetres (55 in). Rasht, the
capital of the province, is known internationally as the "City
of Silver Rains" and in Iran as the "City of Rain". For the
mycorrhizal survey, 20 individual trees were randomly
chosen at four plantation sites in the studied area. Five
root-associated soil samples from each individual plant
were collected, each sample consisting of five bulked sub-
samples (250 cm3 soil cores) randomly collected at 10 to
25 cm in depth. The samples were taken in the summer of
2009.
Isolation and multiplication of AMF fungal spores
The AMF fungal spores were extracted from the
rhizosphere soil by wet sieving and decanting followed by
sucrose centrifugation (Sieverding 1991). After
centrifugation, the supernatant solution was poured
through a 50µm mesh sieve and quickly rinsed with tap
water. Intact and crushed spores were mounted in
polyvinyl alcohol-lactic acid-glycerol (PVLG) and a mixture
of PVLG and Meltzer's reagent (1:1 v/v), to make
diagnostic slides. Spores were grouped, according to their
morphological characteristics and used to initiate cultures
of the different native AMF fungal isolates. For this spore
multiplication process, a sterile mixture of soil/sand (v/v)
was used as substrate for the host plant sorghum
(Sorghum bicolor (L.) Moench). Sorghum seeds were
surface-sterilized (10 min in a 10% diluted commercial
Sodium hypochlorite solution followed by several washes
with sterile water), pre-germinated and transplanted after
emergence into 28 pots and inoculated with about 70
spores of mixture of AMF fungi. Sorghum plants were
grown under greenhouse conditions and maintained for
three months. All plants were provided monthly with 100
ml of Hewitt’s (1952) modified nutrient solution (20 ml
MgSo4 .7H2O/l [18.4 g/l], 10 ml EDTA-Fe/l [2.45 g/l], 1 ml
MnSo4 .7H2O/l [2.23 g/l], 0.1 ml CuSo4 .5H2O/l [2.4 g/l], 0.1
ml ZnSo4 .2H2O/l [2.9 g/l], 0.1 ml H3Bo3/l [18.6 g/l], 0.1 ml
Na2MnO4 .2H2O/l [0.35 g/l], 10 ml KNo3/l [30.3 g/l], 20 ml
Ca [NO3]2/l [70.8 g/l] and 1 ml Na H2Po4 .2H2O/l [20.8 g/l]).
Eight replicates per treatment were prepared. Soil samples
were taken periodically to confirm the AMF colonization.
Morphological identification of AMF fungi
The mixture of AMF fungi spores were identified to species
level. Criteria used were mainly based on spore size and
color, wall structure and hyphal attachment (Walker, 1983;
Morton and Benny, 1990; Schenk and Perez, 1990; Dodd
and Rosendahl, 1996; INAMF, 1997). Permanent slides
were prepared for each isolate using both polyvinyl-alcohol
and polyvinyl-alcohol plus Melzer’s solution (1:1), as
described by Walker (1983).
Molecular identification of Verticillium isolates
Morphological and Molecular identification of Verticillium
isolates The Verticillium isolates were identified according
to conidia and phialides size, microsclerotia characters
and colony characteristics (zare et al., 2002). Verticillium
isolates They were also analyzed by using molecular
approach. Total DNA was extracted according to Raeder
and Broada, 1985 procedure, and used as template for
PCR. ITS sequences were amplified using:
D-1: '5-CATgTTgCTCTgTTgACTgg-3'
D-2:'5-gACACggTATCTTTgCTgAA-3'
3. Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahliae Kleb. on Olive Tree under Greenhouse Conditions
World Res. J. Agric. Sci. 187
The V. dahliae specific primers. The expected specific
band of 548bp were purified using a gel extraction Kit
(Qiagen, Hilden, Germany) and sequenced in both strands
at Macrogen company, Korea. Data sequences were
compared to Gen BANK with BLAST programs.
Evaluation of AMF fungi association with Verticillium
The AMF isolates were assayed for their interaction with
Verticillium isolate. The mixture of four species of AMF
fungi cultures was established and two olives commercial
varieties currently used to produce olive oil in Iran (Zard
and Roghani) were chosen. Rooted micro- propagated
olive plantlets of these varieties were transplanted in
(35×25cm) pots containing a mixture (1:1) was made from
the mycorrhizal inoculums and autoclaved sandy soil.
Thirty-two olive seedling, sixteen were transplanted into
pots containing the previous mixture and other sixteen into
similar pots, containing a 1:1 mixture of autoclaved clay
and sandy soil. From each group, eight seedlings
remained as control, eight were inoculated with V. dahliae,
eight were inoculated with V. dahliae + mycorrhiza and
other eight were inoculated with mycorrhiza alone.
Inoculated and non-inoculated control plantlets were
grown in greenhouse. The effect of mycorrhizal fungi on
olive Verticillium wilt disease traits (leaf number, branch
number, plant height, fresh and dry weight of olive shoots
and roots) were studied in the factorial design with eight
replications.
Data analysis
Data were processed by the analysis of variance (ANOVA)
using the SAS/STAT Version 6 (1990). Appropriate
statistical differences were determined by using the least
significant difference (LSD) test at p<0.05.
RESULTS AND DISCUSSION
Veriticillium wilt of olive trees constitutes a relevant
economic problem because of its world distribution and
host range (Trapero et al., 2011). Arbuscular mycorrhizal
(AM) fungi are the most common types of all mycorrhiza
and their occurrence as root symbionts has been reported
from exceptionally wide range of plants (Porras-Soriano et
al., 2006). In the current research of interaction of AMF
fungi with Verticillium wilt of olive trees in Iran, based on
the morphological characters of spores and mycological
keys, four AMF species were indentified e.g. Paraglomus
occultum, Glomus etunicatum, G. fasciculatum and G.
clarum, among them, P. occultum and G. fasciculatum are
new to olive rhizosphere in Iran (Figure 1). In the present
study, P. occultum (80%) showed the highest frequency
of occurrence followed by G. etunicatum (26%), G. clarum
(20%), and G. fasciculatum (13%). Symptoms of olive wilt
were observed in Isfahan province during summer of 2009.
The incidence of wilt was occurred on several branches
that gradually dried up and the other branches were
destroyed. On the infected branches, petioles were bent
and finally leaves dried and fall down on earth. Browning
and discoloring of the vessels were observed on the
infected branches. From branches of infected trees
Verticillium was isolated. Fungus was grown on PDA, and
the colony was dark and, in the center, microsclerotia were
formed. Colorless conidiophores were simple or branched
verticillate in branched two to four phialides were observed
(Figure 3). Phialides were thicker at the junction and slim
at the end. The average dimensions of phialides were
29.8×2.1 µm, of spores 2.4×1.9 µm and microsclerotia
79 ×34 µm. Koch's postulate was performed on olive
seedlings and symptoms of wilt and browning vessels was
observed after two months. Based on morphological and
molecular characterization, the fungus was identified, as
V. dahliae and this is the first report of V. dahliae from olive
trees in Isfahan province, Iran (Figure 2). The effect of
mycorrhizal fungi on olive Verticillium wilt disease traits
(leaf number, branch number, plant height, fresh and dry
weight of olive shoots and roots) revealed that fresh and
dry weights of roots inoculated with AMF alone and/or both
the symbiotant and pathogenic fungus were significantly
greater than those inoculated with Verticillium alone and/or
none inoculated ones (Table 1 and Figure 4). These
results are in concordance with the studies performed on
the other fruit trees (Vidal et al., 1992). The olive plantlets
were highly susceptible to infection by the defoliating V.
dahliae pathotypes used, as reported by Trapero and
Blanco (1999). The V. dahliae significantly reduced the
number of leaf and shoot, plant height, dry and fresh
weight of the plants compared to the control seedlings
(Tables 1, 2). In comparison to control with inoculated
olive seedling, results revealed that eight months after
inoculation, number of shoots and leaves and plant height
were significantly increased. The results also showed that
plant growth, root fresh and dry weight, shoot dry and fresh
weight were significantly greater with AMF in presence of
Verticillium inoculation over Verticillium alone, or control
when compared (Table 2). The growth rate of mycorrhizal
olive seedlings was greater than that of non-mycorrhizal
seedlings. Higher fresh and dry weights of shoots and
roots in mycorrhizal and non-mycorrhizal olive seedling
might improve the resistance of these seedlings to
Verticillium wilt. Several pathogenic fungi like species of
Fusarium, Rhizoctonia, Pythium, and Verticillium were
found to have higher frequency of occurrence on the
rhizosphere and rhizoplane of non-mycorrhizal plants as
compared to root-zone of mycorrhizal plants (Chliyeh et
al., 2014; Jiménez‐Fernández et al., 2016). Several
phosphorus solubilizing fungi like Aspergillus spp. and
other saprophytes were found to be higher in number on
mycorrhizal roots as compared to non-mycorrhizal roots
(Mamta and Mukerji, 1990). Olive seedlings inoculated
with mycorrhizal fungi G. mosseae and planting them in
soil contaminated with V. dahliae, reduced disease
severity and increased the growth of seedlings as reported
by Karajeh and Al-Raddad, 1999. Tolerance in citrus to
Phytophthora root rot inoculated by G. fasciculatus
appeared to be due to the ability of mycorrhizal roots to
4. Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahliae Kleb. on Olive Tree under Greenhouse Conditions
Khrieba et al. 188
absorb more phosphorus and other minerals (Davis and
Menge, 1980) than non-mycorrhizal roots in P-deficient
soils. Mycorrhizal fungi alone on plant showed a positive
effect on traits as compared with control. It seems that
mycorrhizal fungi can be used as an appropriate solution
to reduce the degree of olive tree Verticillium wilt disease
(Porras-Soriano et al., 2006) if the mycorrhiza could be
produced commercially in each native land. Liu (1995)
investigated the effect of mycorrhizal fungi on verticillium
wilt of cotton and the role of disease resistance. In another
study, G. fasciculatum decreased the number of sclerotia
and infection rate of root rot of peanut caused by
Sclerotium rolfsii, effectively, under the pot conditions
(Krishna and Bagyaraj, 1983). Although many researchers
have noticed that plant diseases are reduced by AMF, few
have conducted further research on the relationship
between the development of the arbuscules or vesicles
and plant disease tolerance/resistance (O'Bannon et al.,
1979) and according to our findings, it seems that AMF
fungi could help the tolerance of olive seedlings to
Verticillium wilt disease.
Figure 1. Spores mycorrhizal fungi of olive tree A. Glomus clarum. Broken mature spores with wall layers sw1, sw2 and
sw3. B. Glomus etunicatum. Spore base with short subtending hypha (sh) spores with wall layers sw1 and sw2. C. Glomus
fasciculatum. Spore base with short subtending hypha (sh) spores with wall layers sw1 and sw2 D. Paraglomus occultum
Spore base with short subtending hypha (sh) with wall layers sw1 and sw2
Figure 2. Agarose gel electrophoresis of PCR Figure 3. A: Phialides and conidiophores of Verticillium dahliae
Products With D1/D2 primers for the detection B: microsclerotia of Verticillium dahliae.
of Verticillium dahliae M: 100bp ladder marker
Sw3
Sw2A B C
D
Sw2
Sw1
Sw1
Sw2
Sw2
Sw1
sh
Sw1
sh
sh
5. Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahliae Kleb. on Olive Tree under Greenhouse Conditions
World Res. J. Agric. Sci. 189
Figure 4. The effect of mycorrhizal fungi on olive root volume and the growth seedling. A (Left to right): Four treatments
in a greenhouse (sterilized soil only (control), mycorrhiza, mycorrhiza + Verticillium and Verticillium + sterilized soil) on
olive B (Left to right): sterilized soil only (control), mycorrhiza, mycorrhizal + Verticillium and Verticillium + sterilized soil)
on olive tree.
Table 1. Interaction of AMF fungi and Verticillium dahliae
on shoot, root fresh and dry weight of olive seedlings.
Treatment
Shoot
fresh
weight
(g/plant)
Shoot
dry
weight
(g/plant)
Root
fresh
weight
(g/plant)
Root
dry
weight
(g/plant)
Control 15.03b
8.39b
2.18c
1.16c
AMF 30.22a
17.6a
11.46a
5.91a
Verticillium +
AMF
19.8 b
10 b
5.04 b
2.44 b
Verticillium
alone
6.42c
3.15c
1.05c
0.33d
LSD (5%) 4.90 2.47 1.50 0.717
Means within columns by the same letter are not
significantly differently at p ≥ 0.05 using LSD test
Table 2. Interaction of AMF fungi and Verticillium dahliae
on number of shoots leaves and plant height of olive
seedlings.
Treatment Number of
shoots
Number of
leaves
Plant
height(cm)
Control 3.37ab
50.1ab
34.6b
AMF 3.50a
74.5a
48.1a
Verticillium + AMF 3.75a
74.1a
44.5ab
Verticillium alone 2b
24.7b
21.2c
LSD (5%) 1.44 31.1 12.2
Means within columns by the same letter are not
significantly differently at p ≥ 0.05 using LSD test.
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