This document summarizes a study that evaluated the effects of Trichoderma harzianum fungus, poultry manure, and palm wine yeast, individually and combined, on the growth and yield of soybeans grown in nematode-infested soil. Field experiments were conducted over two growing seasons. Application of the biological agents significantly increased soybean growth and yield while reducing nematode populations compared to the untreated control. Combining Trichoderma harzianum with poultry manure or palm wine yeast had the best effects on soybean plant height, leaf number, and soil nematode suppression.
Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yi...IJEAB
Trichoderma species are commonly used as effective biological control agents against phytopathogens especially the soil-borne fungi while some isolates are able to ameliorate plant growth. In the present study, Trichoderma fortified compost with different substrates were evaluated to reduce the pre-emergence and post-emergence seedling mortality, diseases of stem and root of chickpea caused by several soil-borne fungal pathogens, including Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii at different growth stages in the field under natural epiphytotic conditions. Among the twenty isolates of T. harzianum, Co-7 showed the most effective antagonist against the test pathogens in dual culture method. In field experiment, subsequently it was used for inoculum preparation with colonized wheat grain and mixed with well-matured decomposed composting materials like, saw dust, cow dung, tea waste, water hyacinth and poultry manure. Trichoderma fortified compost with poultry manure was found significantly effective in reducing pre-emergence and post emergence seedling mortality, disease incidence and disease severity of chickpea in the field. Interestingly, all the treatments significantly increased but Trichoderma fortified compost with poultry manure was the best to boost seed yield and quality.
Effects of used engine oil polluted-soil on seeds’ germination and seedlings’...IJEAB
The ability of Phaselous vulgaris, Zea mays L., Solanum lycopersium and Sorghum saccharatum to germinate and grow in unpolluted soils, 1% w/w and 2% w/w used engine oil polluted soils were investigated. Twenty (20) seeds of each plant species were sown in the various polluted and unpolluted soils and germination were monitored for 7 days, and subsequent growth for 7 weeks. The numbers of germinated seeds were counted daily from the 2nd to the 7th day, and percentage germination recorded. Plants’ growth parameters (shoot heights and leaf area) of the seedlings were assayed and recorded on the 3rd, 5th and 7th week. Percentage germination varied for the various plant seeds. S. saccharatum had the best germination in polluted and unpolluted soil with 100%, 95% and 90% germination as against the least germination 100%, 65% and 25% observed in S. lycopersium in unpolluted, 1% w/w polluted and 2% w/w polluted soils, respectively. In terms of growth, P. vulgaris had the best performance in unpolluted and polluted soils with mean shoot heights of 47.8 cm, 41.3 cm and 28.4 cm as against S. lycopersium with mean shoot heights of 10.8 cm, 5.8 cm and 3.6 cm in unpolluted, 1% w/w and 2% w/w polluted soils, respectively at the end of the study. The results of this study showed that used engine oil inhibited the germination of these seeds in a dose depended manner, and that inhibition of seeds’ germination does not connote inhibition of subsequent growth. This highlights the need to prevent agricultural soil pollution with used engine oil.
Effect of Trichoderma Fortified Compost on Disease Suppression, Growth and Yi...IJEAB
Trichoderma species are commonly used as effective biological control agents against phytopathogens especially the soil-borne fungi while some isolates are able to ameliorate plant growth. In the present study, Trichoderma fortified compost with different substrates were evaluated to reduce the pre-emergence and post-emergence seedling mortality, diseases of stem and root of chickpea caused by several soil-borne fungal pathogens, including Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii at different growth stages in the field under natural epiphytotic conditions. Among the twenty isolates of T. harzianum, Co-7 showed the most effective antagonist against the test pathogens in dual culture method. In field experiment, subsequently it was used for inoculum preparation with colonized wheat grain and mixed with well-matured decomposed composting materials like, saw dust, cow dung, tea waste, water hyacinth and poultry manure. Trichoderma fortified compost with poultry manure was found significantly effective in reducing pre-emergence and post emergence seedling mortality, disease incidence and disease severity of chickpea in the field. Interestingly, all the treatments significantly increased but Trichoderma fortified compost with poultry manure was the best to boost seed yield and quality.
Effects of used engine oil polluted-soil on seeds’ germination and seedlings’...IJEAB
The ability of Phaselous vulgaris, Zea mays L., Solanum lycopersium and Sorghum saccharatum to germinate and grow in unpolluted soils, 1% w/w and 2% w/w used engine oil polluted soils were investigated. Twenty (20) seeds of each plant species were sown in the various polluted and unpolluted soils and germination were monitored for 7 days, and subsequent growth for 7 weeks. The numbers of germinated seeds were counted daily from the 2nd to the 7th day, and percentage germination recorded. Plants’ growth parameters (shoot heights and leaf area) of the seedlings were assayed and recorded on the 3rd, 5th and 7th week. Percentage germination varied for the various plant seeds. S. saccharatum had the best germination in polluted and unpolluted soil with 100%, 95% and 90% germination as against the least germination 100%, 65% and 25% observed in S. lycopersium in unpolluted, 1% w/w polluted and 2% w/w polluted soils, respectively. In terms of growth, P. vulgaris had the best performance in unpolluted and polluted soils with mean shoot heights of 47.8 cm, 41.3 cm and 28.4 cm as against S. lycopersium with mean shoot heights of 10.8 cm, 5.8 cm and 3.6 cm in unpolluted, 1% w/w and 2% w/w polluted soils, respectively at the end of the study. The results of this study showed that used engine oil inhibited the germination of these seeds in a dose depended manner, and that inhibition of seeds’ germination does not connote inhibition of subsequent growth. This highlights the need to prevent agricultural soil pollution with used engine oil.
Changes in Aflatoxins Contents of the Maize (Zea Mays L.) Stored in Clay Gran...Agriculture Journal IJOEAR
Abstract— Maize protection without any risks for human health and environment concerns might be valued on alternative uses of pest control methods that do not only rely on synthetic insecticides. A combination of leaves derived from Lippia multiflora Moldenke and Hyptis suaveolens Poit. Benth were tested for their protective effect on the aflatoxins levels of maize cobs and grains stored in traditional and improved granaries in Côte d’Ivoire. Thus, 4 aflatoxins (B1, B2, G1 and G2) were determined with high performance liquid chromatography according to the official method of AOAC. Results showed presence of afltatoxins in 58% of samples, and specifically aflatoxin B1 from half the samples, with rather higher levels than the reference values of the European Union. The levels of aflatoxins B1, B2, G1 and G2 resulted from both maize cobs and grains treated with biopesticides (from 0.06-0.53 µg/kg to 2.18-50.70 µg/kg) were significantly lower than those recorded with untreated maize of control granaries (ranging from 0.06-0.53 µg/kg to 12.48-346.15 µg/kg). In the treated maize, the aflatoxins levels increased slightly during 6 months of storage, while the untreated maize cobs were with significant increasing of the same toxins traits month after month. For each stage, aflatoxins levels of maize cobs and grains did not differ whether they are treated in traditional or improved granaries with both plant materials.
The estimated risk of exposure in aflatoxins, specifically in total aflatoxins and AFB1, deriving with intakes of maize stored for 6 months are respectively 114.37±2.2 ng/kg body weight/day and 36.21±0.11 ng/kg body weight/day for the untreated granaries and 7.15±0.04 ng/kg body weight/day and 2.12±0.17 ng/kg body weight/day for the treated granaries. These levels are strongly higher than the maximal Reference Value (0.15 pg/kg body weight/day) tolerated for Toxicity exposure. Therefore, it’s necessary to sensitize, on a larger scale, actors of maize path, namely farmers, retailers, processers and consumers about such mycotoxins in maize products for providing health safety to Ivorian populations.
A modern method of agriculture with minimum cost of production and maximum yield using new technological approach,accelerating agricultural output through effective utilization of natural resources practiced under any natural ecosystem.
Toxic Effects of some Pungent Smelling Seed Extracts and Powders on Sitophilu...Premier Publishers
Sitophilus zeamais is a notorious field-to-store pest of maize in the Tropic. The use of chemical insecticides, regardless of its adverse effects has been the major means of managing this pest. In this study, bio-insecticides of plant origin was used to control maize weevil in the store. The dry fruits of Capsicum frutescens, Piper guineense and Aframomum melegueta were extracted with ethanol, using Soxhlet apparatus. The extracts and powders were evaluated on S. zeamais Motschulsky for mortality, oviposition and adult emergence. The long term storage of the treated maize was also investigated. The results obtained shows that all extracts and powders from C. frutescens and P. guineense caused 100 % mortality by day 12 of exposure with all extract concentrations and doses of powder applied. Powders of A. melegueta was able to achieve 100 % mortality only by day 12 of exposure. The extracts also suppressed oviposition and subsequent adult emergence. Extracts and powders of A. melegueta and P. guineense completely prevented infestation and hence damage of the treated maize grains for a period of three months. The result obtained suggests that extracts and powders of the test plants most especially that of P. guineense and C. frutescens can be utilized in protecting stored maize grains from infestation by S. zeamais.
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1Dr. siddhant
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
Aspects of the ecology of fruit flies in ugandaBrian Isabirye
Fruit flies cause about 40% fruit loss in Africa, and about 73% in Uganda. Design of IPM strategies for fruit flies requires knowledge of their biology. Was limited to Nakasinga, 2002; Nemeye, 2005; Okullokwany, 2006. It is not clear how: Diversity has been shaped by hosts, distribution and envital variability.
Highly cryptic and inter-intra-specific morphological variation (Clarke et al., 2005; Drew et al., 2008) among Bactrocera spp. turns out.
Will change in climate alter the suitability and distribution of species?
Productivity of Soybean on Different AgroecosystemsIJEABJ
This study aims to see the growth and productivity of soybeans in different agroecosystems. The study was conducted on paddy field located in Bumi Setia village, Seputih Mataram sub-district, Central Lampung district, and on dryland located in Mandah village, Natar sub-district, South Lampung district, Lampung Province, Indonesia, from April to July 2015. Soybean varieties grown at each location were Grobogan varieties. The variables observed were crop emergence, plant height at harvest, number of plant harvested, number of pods per plant, empty pods, weight of 100 grains, pod pests and productivity. Data were analyzed by t test. The results showed that soybean productivity in dryland was 64.25% lower than productivity in paddy fields. The low yield of soybean varieties of Grobogan in dryland was caused due to drought factor when forming and filling pods. This can be seen from the decrease of weight of 100 grains of soybean seed in dryland up to 51.82% than in paddy field. The status of Grobogan varieties soybean vigor may change from large seed to medium seed if the water requirement is not optimum during the growing season.
( Talking about Herbicides )
History
Prior to the widespread use of chemical herbicides, cultural controls, such as altering soil pH, salinity, or fertility levels, were used to control weeds. Mechanical control (including tillage) was also (and still is) used to control weeds.
Effect of foliar application of water soluble fertilizer on growth, yield and...Innspub Net
The tomato is the one of the most famous crops in Pakistan. It is used and consumed as fresh as well as in processed form. Its botanical name is Solanum lycopersicon Mill. The proposed study had been conducted in the year 2017-2018 in order to find the best combination of water soluble NPK fertilizers as compared to control. Five different types of water soluble fertilizers were collected from different sources and were applied at 5% concentration during the entire growing period of the tomato crop. The experiment was designed using Randomized Complete Block Design (RCBD) with five treatments and three replications of each. Different vegetative, reproductive and bio-chemical parameters were recorded and analyzed statistically at 5% level of significance. The treatments were compared using LSD test. It was concluded that different NPK water soluble fertilizers showed variation in physical and bio chemical parameters in tomato plants as compared to control. The plants showed variation in plant height, No. of fruit per plant, fruit yield per hectare, Individual fruit weight, fruit weight per plant, No. of diseased fruit per plant, date of first harvest, Fruit color Fruit length (cm), fruit size (cm), fruit firmness, total NPK contents, vitamincmg Chlorophyll concentration, pH, (TSS), Electricity conductivity (EC) and Titratable acidity Among all the water soluble treatments, the T3 treatment (WSF 20:20:20) yielded the comparatively better results as compared to other WSF treatments. So it was concluded that T3 was the recommended water soluble fertilizer for tomato.
Groundnut is one of the most important cash crops in our country. It is a low- priced commodity but a valuable source of all the nutrients. Groundnut is the sixth most important oilseed crop in the world. It contains 48-50% of oil and 26-28% of protein and is a rich source of dietary fiber, minerals, and vitamins. The production of groundnut is concentrated in Asia and Africa with 56% and 40% of the global area and 68% and 25% of the global production, respectively
Evaluation of the Efficacy of Herbicides during Transition to Conservation Ag...Premier Publishers
The difficulty of manual hoe weeding presents a major challenge to the adoption of conservation agriculture (CA) by smallholder farmers in Zimbabwe. Herbicide use is known to reduce manual hoe weeding requirements during the season while increasing economic returns. Studies to determine the efficacy of herbicides in maize under CA were carried out in Zimbabwe. The treatments evaluated were:(i) manual hoe weeding (ii) paraquat (0.2 kg a.i. ha-1) (iii) glyphosate (1.025 kg a.i ha-1) (iv) atrazine (1.8 kg a.i. ha-1) (v) glyphosate (1.025 kg a.i ha-1) + atrazine (1.8 kg a.i. ha-1) (vi) glyphosate (1.025 kg a.i ha-1) + atrazine (1.8 kg a.i. ha-1) + metolachlor (1.152kg a.i. ha-1). Greater efficacy of weed control was higher in herbicide treated plots compared to hoe weeding alone. Atrazine combined with other herbicides or alone significantly (P<0.05) suppressed Garlinsoga parviflora, Bidens pilosa and other broadleaf weeds that dominated the weed spectrum at study sites. A tank mix of glyphosate + atrazine + metolachlor had significantly higher (P<0.05) maize grain yield than hoe weeding alone. Results showed that herbicides lowered weeding time requirement and were more effective in controlling weeds than manual hoe weeding alone. Farmers are thus likely to enjoy more net economic benefits if they adopt herbicide use as a weed control strategy in CA systems.
This article is written in the context of the control of weeds in the wheat crop. It includes integrated management of all the weeds of wheat. Written by Muhammad Hassan Student at University Sargodha in the instructions of Dr.Rafi Qamar(Assistant Professor Dept of Agronomy) .
8 ijhaf oct-2017-10-influence of nitrogen fixingAI Publications
A field experiment was conducted for two successive seasons 2012/2013 and 2013/2014 with four replicates at the experimental farm, Faculty of Agriculture, Omdurman Islamic University to determine the effect of inoculation and co-inoculation of symbiotic nitrogen fixing and phosphate solubilizing bacteria on fenugreek (Trigonella foenum-graecum L.) growth and yield. Fenugreek plant variety Kodab was used. Two bacterial strains Sinorhizobium meliloti strain TAL380 and Bacillus megatherium var. phosphaticum strain (BMP), each alone or in combination. Control plants were kept for comparison. The following measurements were taken per plant: number of nodules, dry weight of nodules, shoots and roots, plant height, pod length, number of pods/plant, number of seeds/pod, 1000 seed weight and yield. Results showed that inoculation with TAL380 and BMP each alone significantly increased nodulation and nodule dry weight compared to the control. Inoculation with BMP significantly increased root dry weight, when inoculation with TAL380 significantly increased shoot dry weight and plant height, co-inoculation significantly increased root and shoot dry weight compared to the control. The two bacterial inoculations each alone or in combination significantly increased number of pods and insignificantly increased number of seeds per pod and 1000 seed weight at both seasons. There was no effect of inoculation with bacteria on pod length. Co-inoculation significantly increased fenugreek seed yield at both seasons compared to the control.
Changes in Aflatoxins Contents of the Maize (Zea Mays L.) Stored in Clay Gran...Agriculture Journal IJOEAR
Abstract— Maize protection without any risks for human health and environment concerns might be valued on alternative uses of pest control methods that do not only rely on synthetic insecticides. A combination of leaves derived from Lippia multiflora Moldenke and Hyptis suaveolens Poit. Benth were tested for their protective effect on the aflatoxins levels of maize cobs and grains stored in traditional and improved granaries in Côte d’Ivoire. Thus, 4 aflatoxins (B1, B2, G1 and G2) were determined with high performance liquid chromatography according to the official method of AOAC. Results showed presence of afltatoxins in 58% of samples, and specifically aflatoxin B1 from half the samples, with rather higher levels than the reference values of the European Union. The levels of aflatoxins B1, B2, G1 and G2 resulted from both maize cobs and grains treated with biopesticides (from 0.06-0.53 µg/kg to 2.18-50.70 µg/kg) were significantly lower than those recorded with untreated maize of control granaries (ranging from 0.06-0.53 µg/kg to 12.48-346.15 µg/kg). In the treated maize, the aflatoxins levels increased slightly during 6 months of storage, while the untreated maize cobs were with significant increasing of the same toxins traits month after month. For each stage, aflatoxins levels of maize cobs and grains did not differ whether they are treated in traditional or improved granaries with both plant materials.
The estimated risk of exposure in aflatoxins, specifically in total aflatoxins and AFB1, deriving with intakes of maize stored for 6 months are respectively 114.37±2.2 ng/kg body weight/day and 36.21±0.11 ng/kg body weight/day for the untreated granaries and 7.15±0.04 ng/kg body weight/day and 2.12±0.17 ng/kg body weight/day for the treated granaries. These levels are strongly higher than the maximal Reference Value (0.15 pg/kg body weight/day) tolerated for Toxicity exposure. Therefore, it’s necessary to sensitize, on a larger scale, actors of maize path, namely farmers, retailers, processers and consumers about such mycotoxins in maize products for providing health safety to Ivorian populations.
A modern method of agriculture with minimum cost of production and maximum yield using new technological approach,accelerating agricultural output through effective utilization of natural resources practiced under any natural ecosystem.
Toxic Effects of some Pungent Smelling Seed Extracts and Powders on Sitophilu...Premier Publishers
Sitophilus zeamais is a notorious field-to-store pest of maize in the Tropic. The use of chemical insecticides, regardless of its adverse effects has been the major means of managing this pest. In this study, bio-insecticides of plant origin was used to control maize weevil in the store. The dry fruits of Capsicum frutescens, Piper guineense and Aframomum melegueta were extracted with ethanol, using Soxhlet apparatus. The extracts and powders were evaluated on S. zeamais Motschulsky for mortality, oviposition and adult emergence. The long term storage of the treated maize was also investigated. The results obtained shows that all extracts and powders from C. frutescens and P. guineense caused 100 % mortality by day 12 of exposure with all extract concentrations and doses of powder applied. Powders of A. melegueta was able to achieve 100 % mortality only by day 12 of exposure. The extracts also suppressed oviposition and subsequent adult emergence. Extracts and powders of A. melegueta and P. guineense completely prevented infestation and hence damage of the treated maize grains for a period of three months. The result obtained suggests that extracts and powders of the test plants most especially that of P. guineense and C. frutescens can be utilized in protecting stored maize grains from infestation by S. zeamais.
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1Dr. siddhant
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
Aspects of the ecology of fruit flies in ugandaBrian Isabirye
Fruit flies cause about 40% fruit loss in Africa, and about 73% in Uganda. Design of IPM strategies for fruit flies requires knowledge of their biology. Was limited to Nakasinga, 2002; Nemeye, 2005; Okullokwany, 2006. It is not clear how: Diversity has been shaped by hosts, distribution and envital variability.
Highly cryptic and inter-intra-specific morphological variation (Clarke et al., 2005; Drew et al., 2008) among Bactrocera spp. turns out.
Will change in climate alter the suitability and distribution of species?
Productivity of Soybean on Different AgroecosystemsIJEABJ
This study aims to see the growth and productivity of soybeans in different agroecosystems. The study was conducted on paddy field located in Bumi Setia village, Seputih Mataram sub-district, Central Lampung district, and on dryland located in Mandah village, Natar sub-district, South Lampung district, Lampung Province, Indonesia, from April to July 2015. Soybean varieties grown at each location were Grobogan varieties. The variables observed were crop emergence, plant height at harvest, number of plant harvested, number of pods per plant, empty pods, weight of 100 grains, pod pests and productivity. Data were analyzed by t test. The results showed that soybean productivity in dryland was 64.25% lower than productivity in paddy fields. The low yield of soybean varieties of Grobogan in dryland was caused due to drought factor when forming and filling pods. This can be seen from the decrease of weight of 100 grains of soybean seed in dryland up to 51.82% than in paddy field. The status of Grobogan varieties soybean vigor may change from large seed to medium seed if the water requirement is not optimum during the growing season.
( Talking about Herbicides )
History
Prior to the widespread use of chemical herbicides, cultural controls, such as altering soil pH, salinity, or fertility levels, were used to control weeds. Mechanical control (including tillage) was also (and still is) used to control weeds.
Effect of foliar application of water soluble fertilizer on growth, yield and...Innspub Net
The tomato is the one of the most famous crops in Pakistan. It is used and consumed as fresh as well as in processed form. Its botanical name is Solanum lycopersicon Mill. The proposed study had been conducted in the year 2017-2018 in order to find the best combination of water soluble NPK fertilizers as compared to control. Five different types of water soluble fertilizers were collected from different sources and were applied at 5% concentration during the entire growing period of the tomato crop. The experiment was designed using Randomized Complete Block Design (RCBD) with five treatments and three replications of each. Different vegetative, reproductive and bio-chemical parameters were recorded and analyzed statistically at 5% level of significance. The treatments were compared using LSD test. It was concluded that different NPK water soluble fertilizers showed variation in physical and bio chemical parameters in tomato plants as compared to control. The plants showed variation in plant height, No. of fruit per plant, fruit yield per hectare, Individual fruit weight, fruit weight per plant, No. of diseased fruit per plant, date of first harvest, Fruit color Fruit length (cm), fruit size (cm), fruit firmness, total NPK contents, vitamincmg Chlorophyll concentration, pH, (TSS), Electricity conductivity (EC) and Titratable acidity Among all the water soluble treatments, the T3 treatment (WSF 20:20:20) yielded the comparatively better results as compared to other WSF treatments. So it was concluded that T3 was the recommended water soluble fertilizer for tomato.
Groundnut is one of the most important cash crops in our country. It is a low- priced commodity but a valuable source of all the nutrients. Groundnut is the sixth most important oilseed crop in the world. It contains 48-50% of oil and 26-28% of protein and is a rich source of dietary fiber, minerals, and vitamins. The production of groundnut is concentrated in Asia and Africa with 56% and 40% of the global area and 68% and 25% of the global production, respectively
Evaluation of the Efficacy of Herbicides during Transition to Conservation Ag...Premier Publishers
The difficulty of manual hoe weeding presents a major challenge to the adoption of conservation agriculture (CA) by smallholder farmers in Zimbabwe. Herbicide use is known to reduce manual hoe weeding requirements during the season while increasing economic returns. Studies to determine the efficacy of herbicides in maize under CA were carried out in Zimbabwe. The treatments evaluated were:(i) manual hoe weeding (ii) paraquat (0.2 kg a.i. ha-1) (iii) glyphosate (1.025 kg a.i ha-1) (iv) atrazine (1.8 kg a.i. ha-1) (v) glyphosate (1.025 kg a.i ha-1) + atrazine (1.8 kg a.i. ha-1) (vi) glyphosate (1.025 kg a.i ha-1) + atrazine (1.8 kg a.i. ha-1) + metolachlor (1.152kg a.i. ha-1). Greater efficacy of weed control was higher in herbicide treated plots compared to hoe weeding alone. Atrazine combined with other herbicides or alone significantly (P<0.05) suppressed Garlinsoga parviflora, Bidens pilosa and other broadleaf weeds that dominated the weed spectrum at study sites. A tank mix of glyphosate + atrazine + metolachlor had significantly higher (P<0.05) maize grain yield than hoe weeding alone. Results showed that herbicides lowered weeding time requirement and were more effective in controlling weeds than manual hoe weeding alone. Farmers are thus likely to enjoy more net economic benefits if they adopt herbicide use as a weed control strategy in CA systems.
This article is written in the context of the control of weeds in the wheat crop. It includes integrated management of all the weeds of wheat. Written by Muhammad Hassan Student at University Sargodha in the instructions of Dr.Rafi Qamar(Assistant Professor Dept of Agronomy) .
8 ijhaf oct-2017-10-influence of nitrogen fixingAI Publications
A field experiment was conducted for two successive seasons 2012/2013 and 2013/2014 with four replicates at the experimental farm, Faculty of Agriculture, Omdurman Islamic University to determine the effect of inoculation and co-inoculation of symbiotic nitrogen fixing and phosphate solubilizing bacteria on fenugreek (Trigonella foenum-graecum L.) growth and yield. Fenugreek plant variety Kodab was used. Two bacterial strains Sinorhizobium meliloti strain TAL380 and Bacillus megatherium var. phosphaticum strain (BMP), each alone or in combination. Control plants were kept for comparison. The following measurements were taken per plant: number of nodules, dry weight of nodules, shoots and roots, plant height, pod length, number of pods/plant, number of seeds/pod, 1000 seed weight and yield. Results showed that inoculation with TAL380 and BMP each alone significantly increased nodulation and nodule dry weight compared to the control. Inoculation with BMP significantly increased root dry weight, when inoculation with TAL380 significantly increased shoot dry weight and plant height, co-inoculation significantly increased root and shoot dry weight compared to the control. The two bacterial inoculations each alone or in combination significantly increased number of pods and insignificantly increased number of seeds per pod and 1000 seed weight at both seasons. There was no effect of inoculation with bacteria on pod length. Co-inoculation significantly increased fenugreek seed yield at both seasons compared to the control.
El uso de agentes de control biológico antagonistas del suelo, tales como Trichoderma sp., es reconocido ampliamente como un método de lucha contra enfermedades causadas por hongos del suelo. Diversas cepas han sido evaluadas, determinando su efecto inductor de mecanismos de defensa local y sistémico, por medio de interacciones entre el antagonista con las raíces, conduciendo a la acumulación de moléculas de defensa, como las peroxidasas. Al efecto, para evaluar la presencia de diferentes compuestos inductores de la resistencia a diversas enfermedades se realizó la presente actividad de laboratorio, evaluando las cepas de Trichoderma sp. LIG064 y LIG006. El material para la extracción de proteínas e isoenzimas, y la separación mediante electroforesis en geles de poliacrilamida SDS-PAGE, se realizó siguiendo el protocolo modificado citado por Salazar et al. (2011). En general, la lectura de las bandas permitió diferenciar la presencia de elementos inductores de control biológico en las cepas de Trichoderma sp. evaluadas.
Effect of Isolate of Trichoderma Sp. And Incubation Period to Glucose Productioninventy
This study was carried out to determine the effect of isolates of Trichoderma sp. and the incubation period to glucose production. A glucose produced by the three isolates of Trichoderma sp. (TV.0209, TV.0305 and TV.0710) which were obtained from the collection of the Laboratory of Microbiology, Department of Chemical Engineering of The State Polytechnic of Malang. It was determined that the isolates had a significant correlation in producing glucose with incubation period for 10 days. Data were then analyzed using two-way anova with the alpha of 5% and using Microsoft Excel. Isolate TV.0209 was capable to produce the highest average of a total glucose production (3,616 ppm) when compared with TV.0305 (3,253 ppm) and TV.0710 (2,448 ppm). The optimum incubation period of treatment isolate variables was achieved during 6 days.
In vitro evaluation of Trichoderma viride and Trichoderma harzianum for its e...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Utilisation of non-edible, renewable lignocellulosic biomass for the production of second generation biofuels and chemicals is hindered especially by the high price of enzymes needed for biomass degradation. Filamentous fungi are natural producers of enzymes active against plant cell wall polymers. Especially the ascomycota fungus Trichoderma reesei is widely utilised in the industry for the production of cellulases and hemicellulases. However, the efficiency of enzyme production needs to be further improved in order to ensure economical production of biobased products. Several environmental factors affect protein production by filamentous fungi. Cellulase and hemicellulase genes of T. reesei are activated by inducer molecules derived from different substrates. The need for cooperation of different hydrolytic enzymes for the total degradation of plant cell wall material has led to coordinated expression of these genes. However, the extent and timing of induction can vary between different genes and especially the hemicellulase genes are differentially induced by various substrates. The direct regulation of cellulase and hemicellulase genes by transcriptional regulators has been widely studied and several activators and repressors of these genes have been characterized in detail. However, little is still known concerning the exact regulatory pathways and mechanisms utilised by the fungus for the accurate timing and composition of the hydrolytic enzymes produced.
In this study, a genome-wide transcriptional analysis of T. reesei gene expression at different ambient pH conditions was conducted in order to identify genes affected by extracellular pH. The role of a T. reesei orthologue for the characterized pH regulator, PacC, in the expression of cellulase and hemicellulase genes was also studied. An extensive induction experiment together with transcriptional profiling was then utilised to study the effects of several different substrates on the expression of genes encoding carbohydrate active enzymes (CAZy). In addition, transcriptomics data was utilised for the identification of novel candidate regulators affecting cellulase and xylanase production by T. reesei.
Transcriptional profiling identified pH as an important determinant of T. reesei gene expression. Ambient pH was also found to affect the expression of several cellulase and hemicellulase genes and more information on the role of a PacC orthologue in the expression of cellulase and hemicellulase genes was gained. A profiling study utilising different substrates as inducers together with a thorough annotation of the T. reesei CAZy genes revealed the expression patterns of novel candidate genes possibly involved in the degradation of different types of cellulosic and hemicellulosic substrates.
Poster at the 4th International Rice Congress
Authors: Febri Doni, Anizan Isahak, Norela Sulaiman, Che Radziah Che Mohd Zain, Abidah Ashari, Wan Mohtar Wan Yusoff
Title: Use of Tricoderma spp. in Enhancing Rice Productivity
Venue: Bangkok International Trade and Exhibition Centre (BITEC), Bangkok, Thailand
Date: October 28-31, 2014
Interaction between Arbuscular Mycorrhiza Fungi (AMF) with Verticillium dahli...Premier Publishers
Vericillium wilt of olive tree (Olea europaea L.) is one of the most important diseases of olive plantations worldwide. Four distinguishable native AMF species (Paraglomus occultum (C. Walker), Glomus etunicatum W.N. Becker & Gerd. Glomus fasciculatum (Thaxt.) Gerd. & Trappe and Glomus clarum T.H. Nicolson & N.C. Schenck) were morphologically identified. The effectiveness of these native AMF fungi as inoculants for two target varieties of olive (Roghani and Zard), currently used in Iran was assessed. The current study in interaction of AMF fungi with Verticillium dahliae kleb. of olive inoculated with four different AMF fungi were used to suppress Verticillium wilt under greenhouse conditions. The fresh and dry weight of shoots and roots and the rate of increase in plant height and leaf number were significantly greater for olive seedlings inoculated with both AMF and Verticillium. No significant differences were observed between growth of olive seedlings inoculated with V. dahliae and control. This study supports the need to explore and exploit the natural diversity of AMF fungi as a starting point to formulate inoculants to be applied during the commercial nursery production of olive varieties.
Evaluation of four plants compost teas against fungi responsible for Corn dam...IJEABJ
Corn (Zea mays L.) is one of the most important cereals in Côte d'Ivoire. However, Corn seeds and seedlings are susceptible to infection by a number of soilborne fungi which caused seeds decay before or after germination. The objective of this study is to evaluate the suppressive effect of Four plants compost teas (Chromolaena odorata, Ricinus communis, Nicotiana tabacum, Azadiracta indica) on Corn damping off. In vitro assays showed a most suppressive effect of C. odorata and R. communis compost teas on mycelial growth of pathogenic fungi. Results of in vivo trials showed significant reduction of Corn seedlings diseases incidence and high seed germination percent after treatment with C. odorata, R. communis and A. indica compost teas. No efficiency effect was noted with N. tabacum compost tea. This study demonstrated the usefulness of compost tea as an efficient biological tool for the control of fungi responsible of corn damping-off.
Synergetic effects of various plant extracts as bio-pesticide against wheat a...Muhammad Qasim
Human health and environment are greatly affected by extensive used of synthetic insecticide, which is why the alternative way of pesticides, such as botanical pesticide consumption increase with the passage of time to control of insect pests. The insecticidal impact of Moringa (Moringa oleifera), Neem (Azadirachta indica) and Euclyptus (Eucalyptus globules) leaves extracts were investigated on Diurophous noxia (Hemiptera: Aphididae). This study was aimed to evaluate the efficiency of bio-pesticide instead of synthetic chemicals against D. noxia. Experimental results showed that the percentage of mortality fluctuate with the fluctuation of concentration with time intervals and have great impact on the population of D. noxia. Single, doubled and tripled combinations of leaves extract were tested at two different concentrations (25 and 50%). The results suggested and concluded that from all the application of single botanicals A. indica give better result as compared to other single application while application of double combination M. olerifera with A. indica give significance result instead of other double mixture, meanwhile the triple combination (M. olerifera, A. indica and E. globules) performed excellent result as the others triple mixtures. The study revealed that combine used of M. olerifera, A. indica and E. globules leaves extract were very effective against D. noxia and surge as an alternative way of pesticides instead of synthetic chemicals.
Growth and Yield Response of Bread Wheat Variety Grown Under Varying Seed Rat...Premier Publishers
Wheat is among the most important staple crop globally. However, constrained by appropriate agronomic practices. Therefore, the information on the interaction effect of seed rate and weeding period is useful to identify the effective time of weeding for high yield of wheat. Thus, the present study conducted at Amuru district of Horro Guduru Zone, Ethiopia in 2019 cropping season with the aim of identifying optimum seed rate and appropriate time of weeding to improve production and productivity of bread in the area. The experiment was laid down in randomized complete block (RCB) design with three replications. The treatment was arranged in factorial combinations of four weeding intervals (farmer practice, weeding at two weeks after emergence, three weeks after emergence and four weeks after emergence) and three levels of seed rate (125 kg, 150 kg and 175 kg-1).The result showed that days to 50% heading, days to maturity and effective tillers per plant were highly significantly (p<0.01) affected by the interaction effect of weeding time and seed rate. Moreover, interaction effect of weeding time and seed rate was significantly (p<0.01) affected the weed above ground dry biomass. Guizotia scabra (22.47%) with population density (370), Phalaris paradoxa (22.10%) with population (364), Plantago lanceolata (18.58%) with population density (306), and Bidens piloso L. (8.74%) were the dominant weed species competing with wheat in the study area. Minimum relative weed density (26.6%) weed dry biomass (1.7gm) and maximum weed control efficiency (98.08%) was recorded at weeding four weeks after emergence and 175kgha-1seed rate. Thus, the finding suggest grain yield was increased (52.3%) when weeding four weeks after emergence over farmers practice and 13.75% at 175kg seed rate.
Weed control is an important agronomic practice that improves growth and maximizes yield in maize. An experiment was carried out to investigate the ‘effect of different weed control methods on the growth and yield of maize (Zea mays L.) in the western highlands of Cameroon. The work was carried out during the 2017/2018 main cropping season from the 14th of March to the 14th of July at the Institute of Agricultural Research for Development (IRAD) Bambui experimental field. The experiment was laid out in a Randomized Complete Block Design (RCBD) and replicated three times. The experiment comprised of seven treatments: weedy check or control (T1), constant hand hoeing (T2), delay hand hoeing (T3), pre-emergence herbicide application of Atrazine 90 DF ( 0.9 kg ha-1) (T4), post-emergence herbicide application of Ultramais 100 SC (1.9 litre ha-1) (T5), pre- herbicide application of Atrazine 90 DF ( 0.9 kg ha-1) + Ultramais 100 SC (1.9 litre ha-1) (T6) and delay post-emergence herbicide application of Ultramais 100 SC (1.9 litre ha-1) (T7). The white maize variety CHC 201 (“KASAI”) was used during the experiment and sown in plot sizes of 4 m x 5 m with a planting density of 80cm by 50cm with 2 plants per station with the aim of achieving a plant population of 50,000 plants ha-1. All agronomic practices were followed from planting to harvesting. The data recorded were plant height, number of green leaves per plant, leaf area, stem girth, days to 50% tasseling, days to 50% silking, ear height, ear length, ear girth, number of gain rows per ear, number of gains per ear, 1000 grain weight and grain yield.. The data collected were analyzed with the aid of STATGRAPHICS Plus 5.0 Software and ANOVA was run to find the differences between the various treatments. The highest 1000 grain weight (314.13g) came from the pre-emergence plus post-emergence herbicide application treatment and did not differ significantly (P˃0.05) from the constant hand hoeing treatment (307.83g). The lowest 1000 grain weight (234.67g) was seen in the weedy check treatment. The constant hand hoeing had the highest grain yield (6.27 ton ha-1) and this did not differ significantly (P˃0.05) from the pre-emergence plus post-emergence herbicide application treatment (6.07 ton ha-1). The lowest grain yield (3.18 ton ha-1) was seen in the weedy check treatment. From the study, the pre-emergence plus post-emergence herbicide application may be recommended for increasing maize yield particularly in the case of high scale production.
Application of certain bacterial and fungal species for controlling Meloidogy...Premier Publishers
Bacillus subtilis (Bs) and B. megaterium (Bm), Pseudomonas fluorescens (Pf) as well as Trichoderma harziamum (Th), Trichoderma vierns (Tvr)and T. viride (Tv) were applied alone as well as in combination in pot experiment for controlling M. incognita in Cowpea. Results showed that Bs, Bp and Pf had nematicidal effects against M. incognita parameters viz., J2 in soil and roots, galls and egg-masses compared to untreated check. Bm alone had highly nematicidal activity against J2 in soil, while Bs was the best against J2 in roots, galls and egg-masses. Bs + Bm in combination was effective against M. incognita parameters. The tested species of Trichoderma also reduced the parameters of M. incognita.Th alone was highly effective in reducing the tested nematode parameters, followed by Tvr and Tv treatments. Tv + Tvr highly reduced the numbers of J2 in soil and roots, while Th+Tv+Tvr significantly reduced the galls and egg-masses numbers. All treatments also increased the growth parameters of cowpea viz., shoot length, fresh and dry shoot weights, and leaf numbers.
Influence of fertilizers on incidence and severity of early blight and late b...Innspub Net
The potato (Solanum tuberosum) production in the Far North Region, Cameroon is confronted with, diseases and pests. To improve the production of this plant, a study was carried out in Mouvou and Gouria to evaluate the impact of fertilizers on the development of late blight and early blight diseases of this plant. The experimental design used was a completely randomized block with 4 treatments: Mycorrhizae (MYC), NPK (20-10-10) chemical fertilizers, chicken droppings (CD) and a control (T). The plant material used was a local variety of potato (Dosa). Disease incidence and severity and rainfall were evaluated. Area Under Disease Progress Curve was calculated. At 60 DAS, mean incidences recorded for fertilizers were 5.7, 3.6, 1.8 and 0.8 % respectively for control, MYC, NPK and CD. In general, early blight severity decreased from 22.1% at 45 DAS to 0.3 % at 60 DAS. The highest AUDPC value of late blight at Mouvou site was observed in NPK treatment while potato in CD treatment had the lowest. The lowest AUDPC value of early blight was observed in CD treatment at both sites. AUDSIPC value for late blight was significantly higher in NPK treatment in both sites. The highest value of AUDPSIC of early blight was recorded in MYC treatment, 45 DAS in both sites. The average rainfall was higher in the Gouria site (716.5mm) than in Mouvou site (679 mm). The CD treatment can be recommended to the farmers for the phytosanitary protection of potatoes.
Evaluation of rice genotypes for resistance to the stalk-eyed fly (Diopsis lo...Innspub Net
Globally, rice production is limited by abiotic and biotic factors. Of the insect pests attacking rice, the stalk-eyed fly is the most abundant. Major rice growing districts in Uganda are affected, and varieties grown by farmers are susceptible. The objective of this study was to identify sources of resistance to stalk-eyed flies among improved rice genotypes in Uganda. Fifty genotypes from the Africa Rice Centre, IRRI, South Korea and the National Crops Resources Research Institute (NaCRRI) in Uganda were screened under cage and field conditions at NaCRRI. Trials were laid out in an alpha lattice design, with 3 replications, for both experiments. Natural infestation (D. longicornis or D. apicalis) was used in the field while cage trials utilized artificial infestation with D. longicornis. Data on deadhearts were collected from seedling to tillering stages, at 7, 14, 21 and 28 days. Analyses of variance were performed using restricted maximum likelihood. Infestation levels for 31 (62%) rice genotypes were the same under both field and cage conditions, 4 (8%) genotypes showed higher susceptibility in the cage than in the field and 15 (30%) were more resistant in the cage than in the field. Genotypes NERICA 4, TXD306, NM7-22-11-B-P-1-1 and K85 were identified as the most resistant varieties. F3 genotypes (GSR IR1- 5-S14-S2-Y1 x K85, Gigante x NERICA4, NERICA4 x Gigante, NERICA1x NERICA4, NERICA4 x NERICA6, and NERICA4 x SUPA) were also found resistant. These genotypes were recommended for release and further advancement, respectively.
MICROORGANISMS ASSOCIATED WITH THE SPOILAGE OF CUCUMBER, GARDEN EGG AND PAWPA...paperpublications3
Abstract: A total of nine cucumbers, each of Garden egg and pawpaw samples were collected from Wurukum, High level and Wadata markets and cultured on appriopate agar, for colony count and isolation of bacteria according to their cultural and biochemical characteristics. The results revealed that garden egg from High Level Market had the highest bacterial count (1.9x105cfu/g) and the least was pawpaw from High Level Market (1.1 x 105 cfu/g). However, it was not statistically significant. The bacteria isolated were; Propianol bacteria (23.3%), Escherichia coli (16.6%), Staphylococcus aureus (36.7%), Bacillus spp (10.0%) and Corynebacteria (13.3%). The fungal isolates were Aspergillus flavus (10%), Aspergillus fumigatus (20%), Aspergillus nidulus (10%), Aspergillus terreus (20%) and mucor (40%). The result of this study shows fruits sold in Wurukum, High Level Market and Wadata Market are contaminated and may cause harm to consumers, so measures such as proper handling should be taken to control the contamination of these fruits.
Effect of arbuscular mycorrhiza fungi on the growth, nutrient uptake, root in...Innspub Net
The effect of Arbuscular mycorrhiza (AM) fungi on growth, nutrient uptake and root infectivity was determined in Rough Lemon (Citrus jambhiri) seedlings raised under four phosphorus regimes in sand culture and also in sand/nitrosol sterile and unsterile conditions. Inoculation with AM fungi increased the plant height, leaf number and stem girth in relation to un-inoculated seedlings grown under equivalent P concentrations. An increase in plant height, leaf number and stem girth also occurred in both inoculated sterile and un-sterile sand/nitrosol media in relation to un-inoculated sterile and unsterile media. Arbuscular mycorrhiza also increased the leaf area and the root, leaf and stem fresh and dry weights and also caused an increase in the uptake of phosphorus and potassium in the leaf tissues. It also favoured mycorrhizal infectivity of roots and increased the root absorptive surface area. This study indicates that AM fungi improves the capacity of tropical fruit to absorb and utilize plant nutrients possibly by increasing the effective root surface area from which available form of nutrients are absorbed and also by increasing access of roots by bridging the depletion zones. Inoculating seedlings with arbuscular mycorrhizal fungi helps to alleviate the adverse effects of global warming and climate change. As a low-cost technology, arbuscular mycorrhizal inoculation is recommended as part of the regular practice for incorporating into nursery media used for tropical fruit seedling propagation.
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Efficacy of trichoderma harzianum, poultry manure and yeast on the growth and yield of soybean grown on nematode infested soil
1. Journal of Natural Sciences Research
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
Vol.3, No.10, 2013
www.iiste.org
Efficacy of Trichoderma harzianum, Poultry manure and Yeast on
the Growth and Yield of Soybean Grown on Nematode Infested
Soil
1.
2.
Timothy Ipoola Olabiyi1*, Oladiran Jeremiah Ojo1, James Olugbenga Adisa1, Michelina Ruocco2
Department of Crop and Environmental Protection, Ladoke Akintola Univeristy of Technology, P.M.B
4000, Ogbomoso, Nigeria.
Istituto per la Protezione delle Piante, CNR, Via Università 130, Portici (NA), 80055, Italy.
*Email of the corresponding author: t.olabiyi@yahoo.co.uk; tiolabiyi@lautech.edu.ng
Abstract
Field experiments were carried out during 2010 and 2011 planting seasons in order to assess the effect of
Trichoderma harzianum, cured poultry manure and palm wine yeast, singly and in combination, on the growth
and yield of soybean, variety TGx 536-02D, grown on nematodes infested soil. The treatments were control, T.
harzianum T22 isolate, cured poultry manure, palm wine yeast, T. hazianumT22 isolate + cured poultry manure,
T. hazianumT22 isolate + palm wine yeast, cured poultry manure + palm wine yeast and T. hazianumT22 isolate
+ cured poultry manure + palm wine yeast. Control experiment did not receive cured poultry manure, palm wine
yeast or T. hazianumT22 isolate. In each trial, there were 8 treatments replicated 5 times fitted into randomized
complete block design. The results indicated that application of T.hazianumT22 isolate, cured poultry manure,
palm wine yeast, T. hazianum + cured poultry manure, T. hazianum + palm wine yeast, cured poultry manure +
palm wine yeast and T. hazianumT22 isolate + cured poultry manure + palm wine yeast significantly (p<0.05)
increased the growth and yield of soybean, and also significantly (p<0.05) reduced the soil population dynamics
of nematode pests of soybean. Prominent nematode genera were Meloidogyne, Xiphinema and Helicotylenchus.
Control plants that were not treated with palm wine yeast, T. harzianum T22 isolate and cured poultry manure
had significantly (p<0.05) reduced the growth and yield of soybean,and had increased soil nematode population.
Data collected on both trials were analyzed using analysis of variance and significant differences among
treatments were separated using Duncan’s multiple range test at probability level of 5%.
Keywords: Trichoderma harzianum; poultry manure; yeast; soybean; control; nematode.
1.
Introduction
Soybean (Glycine max (L.) Merrill) thrives very well in the tropical, subtropical and temperate climates.
Soybean is an important high quality and expensive protein and oil sources. It has an average protein content of
40% and oil content of 20%. It also has a superior amino acid profile.
Soybean protein has a great potential as a major source of dietary protein. The oil produced from soybean is with
a highly digestible and contains no cholesterol. A by-product from the oil production (soybean cake) is used as
high-protein animal feed in many countries. Soybean also improves soil fertility by adding nitrogen to the soil
from the atmosphere.
Compost utilization as manure is becoming wide spread during recent years as consequences of the rise in price
of conventional fertilizers. Beneficial effects of Trichoderma harzianum and organic fertilizer application on the
growth and yield of some field crops have been established (Radwan & Hussein, 1996; Mekki et al., 1999).
Nowadays, more emphasis has already been placed on research and development activities that led to the
concept of multi-strain T. hazianum, that have definite beneficial well-known role in supporting plant growth in
the developing sustainable soil fertility and in bio-controlling soil borne diseases. Palm wine yeast as a natural
bio-stimulant has appeared to reduce astonished influences on growth and yield of some crops (El-kholy &
Gomaa, 2000)
Plant parasitic nematodes are major agricultural pests worldwide responsible for global agricultural losses
(Karssen & Moens, 2006).Nematodes live in the soil and they are widely distributed or spread and persist as soil
plant pest for indefinite period (Caveness, 1976; Whiting et al., 2007).All crops grown in Nigeria are prone to
nematode attack, causing farmers’ significant crop loss annually (Oyedunmade et al., 2009). Farmers are not
always aware of losses being caused as a result of nematodes because they are hidden from sight and at times
misconstrue to be loss from other pathogenic organism, nutrient loss or certain environmental factors. Plant
parasitic nematodes are microscopic organisms that feed on plant roots. Nematodes cause damage to roots,
resulting in root systems which are less able to take up nutrients and water. They live in the soil and plant tissues
(Wang & McSorley, 2005; Westerdah & Kodira 2007).
The objective of this research work was to assess the effects of Trichoderma harzianum, cured poultry manure
and palm wine yeast, singly and in combination, on the growth and yield of soybean grown in nematode infested
42
2. Journal of Natural Sciences Research
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
Vol.3, No.10, 2013
www.iiste.org
soil.
2.
Materials and Methods
In 2010 and 2011 planting seasons, naturally infested field of the Teaching and Research Farm of the Ladoke
Akintola University of Technology, Ogbomoso, Nigeria was sown with nematode susceptible soybean variety
TGx 536-02D. The attempt was to assess the effect of T. harzianum, cured poultry manure and palm wine yeast,
singly and in combination, on the growth and yieldof soybean sown on nematode infested soil. The soil used for
this experiment had been previously confirmed as being naturally nematode infested (Oyedunmade et al., 2009)
and TGx 536-02D soybean variety that was used as test crop had been confirmed to be nematode susceptible
(Oyedunmade & Olabiyi, 2004).The soybean seeds were obtained from International Institute of Tropical
Agriculture (IITA), Ibadan, Nigeria. There were 8 treatments, each replicated 5 timesand fitted into randomized
complete block design. The treatments and application rates were T. harzianum (1x107 spores); cured poultry
manure (10g); palm wine yeast (1x107 spores); T. harzianum (1x107 spores) + cured poultry manure (10g); T.
harzianum (1x107 spores) + palm wine yeast (1x107 spores); palm wine yeast (1x107 spores) + cured poultry
manure (10g); T. harzianum (1x107 spores) + palm wine yeast (1x107spores) + cured poultry manure (10g) and
control.Composite soil samples were collected from the experimentalplots; the soil was air-dried, crushed and
sieved to remove stones, and thereafter analyzed in the laboratory.
Two seeds were planted per stand which was later thinned down to one healthy seedling per stand 2weeks after
planting. Also at two weeks after planting and during both trials, the experimental plots were inoculated with
pure cultured nematodes obtained from International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.
Weeding and other agronomic practices were manually done as at when due. Palm wine yeast was obtained
through palm wine yeast cultured on nutrient agar while T. harzianum T 22 isolates was supplied from Istituto
per la Protezione delle Piante, CNR, Via Università 130, Portici (NA), Italy. The poultry manure used was well
cured for a period of 5 months before use. Treatments were applied at 3 weeks after planting.
The growth and yield parameter measured include plant height, number of leaf per plant, root length, number of
branches/per plant, number of flower/plant, pods weight/ plant, number of pods / plant, number of seeds/pod,
seeds weight/plant and seed weight.Final soil nematode populations and identification were obtained (Whitehead
& Hemming, 1965; Eisenback et al., 1981; Byrd et al., 1983).Data collected on both trials (2010 and 2011) were
analyzed using analysis of variance and significant differences among treatments were separated using Duncan’s
multiplerange test (DMRT) at 5% probability level.
3. Results
The result presented on Table 1 shows the physical and chemical composition of the soil used for the experiment
in both trials. The soil texture was sandy loam with pH 6.8. The soil has low nutrient status.The soil has 9.5%
organic carbon, 16.4% organic matter, 1.7% nitrogen, 0.66mgKg-1and 0.38molKg-1 calcium. The soil also has
very low micro-nutrients. Iron, magnesium and zinc were as low as 0.3, 1.05 and 1.8 mgKg-1.
Table 2 elicits the effects of T. harzianum, cured poultry manure and palm wine yeast, singly and in combination,
on the growth of soybean sown on nematode infested soil. The application of palm wine yeast, T. harzianum +
cured poultry manure and palm wine yeast + cured poultry manure significantly (p<0.05) increased the plant
height more than other treatments; this was closely followed by soybean that received T. harzianum, cured
poultry manure, T. harzianum + palm wine yeast, T. harzianum + palm wine yeast + cured poultry manure
treatments. However, T. harzianum + cured poultry manure significantly (p<0.05) enhanced the number of
leaves per plant, this was closely followed by palm wine yeast + cured poultry manure treatment. Control plant
had the least growth rate. It was evident that the effects of different treatments on the growth of soybean, sown
on nematode infested soil, followed the same trend at both trials.
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4. Journal of Natural Sciences Research
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Table 2: Effects of Trichoderma harzianum, cured poultry manure and palm wine yeast on the growth of
soybean sown on nematode infested soil.
Table 3: Effects of Trichoderma harzianum, cured poultry manure and palm wine yeast on the yield of soybean
sown on nematode infested soil.
Table 4: Effects of Trichoderma harzianum, cured poultry manure and palm wine yeast on the final nematode
population of soybean sown on nematode infested soil
The result on Table 3 shows that T. harzianum at the rate of 1x107 spores per plant; cured poultry manure at the
rate of 10 g per plant; palm wine yeast at the rate of 1x107spores per plant; T. harzianum at the rate of 1x107
spores + cured poultry manure at rate of 10g per plant; T. harzianum at the rate of 1x107 spores + palm wine
yeast at the rate of1x107spores; palm wine yeast at the rate of 1x107 spores + cured poultry manure at the rate of
10g per plant; T. harzianum at the rate of 1x107 spores + palm wine yeast at the rate of 1x107 spores + cured
poultry manure at the rate of 10g per plant significantly increased number of flower, pod number, number of
seed and the weight of soybean seeds at both experimental trials. The control plants had significantly lower
number of flower per plant, pod number per plant, number of seed per pod, and the weight of soybean seeds than
the soybean that received treatments.
Table 4 shows the population dynamic of the soil inhabiting nematodes dynamics grown with soybean. It was
evident that the species of nematodes present in the experimental plots were Meloidogyne, Xiphinema and
Helicotylenchus. The T. harzianum, cured poultry manure and palm wine yeast, singly and in combination,
reduced the population of the soil inhabiting parasitic nematodes present in the experimental plots at both trials.
The experimental plots that did not receive any treatment (control plots) have higher number of soil inhabiting
plant parasitic nematodes.
4. Discussion
The findings corroborate the earlier works of some scientists (Gomaa, 1995; El-kholy & Gomaa, 2000), who
reported that Trichoderma harzianum could replace 50% of the chemical fertilizer recommended for millet
plants without decreasing the green and dry fodder, this could be attributed to the plant growth promoting
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5. Journal of Natural Sciences Research
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
Vol.3, No.10, 2013
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substances produced by the T. harzianum, in addition to the reasonable quantity of atmospheric nitrogen fixed by
the T. harzianum.Application ofcured farm yard manure had resulted into increased number of branches/plant
and significant increased (21%) in final grain yield of wheat (Badaruddin et al., 1999). Application of
Trichoderma harzianum associated with organic manure on groundnut had resulted in an increase in yield and its
components (Ahmed et al., 1997).
Trichoderma spp. are widely known in plant agriculture, both for disease control and yield increases, even under
axenic conditions (Lo et al., 2000; Yedidia et al., 2001; Harman, 2006). Trichoderma species have evolved
multiple mechanisms that result in improvements in plant resistance to disease and plant growth and productivity
(Vinale et al., 2008). Possible explanations of this phenomenon include: control of minor population of
pathogens leading to stronger root growth and nutrient uptake, secretion of plant growth regulatory factors such
as phytohormones and release of soil nutrients and minerals by increased saprophytic activity of Trichoderma in
the soil (Ousley et al., 1994; Yedidia et al., 2001; Celar & Valic, 2005; Muthukumar et al., 2005; Harman, 2006).
Moreover, recent studies have indicated that these fungi also induce localized or systemic resistance systems in
plants (Yedidia et al., 1999; Hanson, 2000; Howell, 2003).
5.
Conclusion
From the results obtained in this study, it could be concluded that Trichoderma harzianum, cured poultry manure
and palm wine yeast, applied either singly or in combination enhanced the growth and yield of soybean sown in
nematode infested field. The application of Trichoderma harzianum, cured poultry manure and palm wine yeast,
being eco-friendly, would be good measures that could enhance the growth and yield of soybean in a nematode
infested field.
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