This study evaluated a novel "inside-out" radiofrequency ablation technique for rapidly localizing the long head of the biceps tendon (LHBT) during arthroscopic biceps tenodesis. The technique was performed on a cadaver specimen and involved creating an anterosuperior portal near the biceps pulley under arthroscopic visualization, inserting a radiofrequency device into the bicipital groove, and dividing the transverse humeral ligament from distal to proximal under direct vision. Open dissection confirmed the technique caused no injury to the rotator cuff tendons. This technique offers a rapid method for LHBT localization without extensive bursectomy to visualize the tendon and may reduce risks of i