Aim :- Aim is to find out the rate of glucose absorbed across an everted sac prepared from the rat intestine using the drugs Beta- caryophyllene and L-Arginine. Result :- The combination of BCP & LA showed significant decrease in glucose absorption in small intestine. Thus, we conclude that this combination approach (LA + BCP) possesses the new therapeutic regimen for the treatment of T2DM.

1. PRACTICE SCHOOL
INVITRO GLUCOSE ABSORPTION BY EVERTED GUT SAC METHOD
KUNJ FOSI
DEVIN BANERJEE

2. AIM OF THIS EXPERRIMENT :
PRINCIPLE :
Aim is to find out the rate of glucose absorbed across an
everted sac prepared from the rat intestine using the
drugs Beta- caryophyllene and L-Arginine.
The Glucose transporters (GLUT) present in the intestine transports the glucose
passively. Passive transport of glucose through the cellular membrane is otherwise
catalysed by glucose carriers (protein symbol GLUT), GLUT 2, a transmembrane
carrier protein of GLUT family, located in the basolateral membrane of the small
intestine and is a very efficient carrier of glucose across the small intestine level.

3. INTERSTINAL GLUCOSE
ABSORPTION :
Source : Intestinal Fructose and Glucose Metabolism in Health and disease (Review paper)

4. PREPARATION OF EVERTED GUT
SAC :
Source : Glucose transport into everted sacs of the small intestine of mice (Review paper)

5. PROCEDURE :
 Prepare everted gut sac
 Fill the everted gut sac with KHB buffer of
pH 7.4
 Now place the sac into the Volumetric
flask containing diff combination of
solutions, maintained at 37 ̊C.
 The study was designed into four groups
and was treated as follow:
1) Group 1: Control KHB + Glucose (2%) + everted
sac
2) Group 2: Test KHB (2%) + Glucose (2%) + LA (500
µg/ml) + everted sac
3) Group 3: Test KHB (2%) + Glucose (2%) + BCP
(500 µg/ml) + everted sac
4) Group 4: Test KHB + Glucose (2%) + Combination
of LA & BCP (500 µg/ml each) + everted sac
 Incubate for atleast 90 min.
 Determine glucose conc of mucosal as
well as serosal sol using glucometer.
Source :- Insulinotropic and anti-diabetic effects of β-caryophyllene with L-arginine in type-2 diabetic rats (Review Paper)

6. OBSERVATION :
Source :- Insulinotropic and anti-diabetic effects of β-caryophyllene with L-arginine in type-2 diabetic rats (Review

7. RESULT :
Source :- Insulinotropic and anti-diabetic effects of β-caryophyllene with L-arginine in type-2 diabetic rats (Review
The combination of BCP & LA showed significant decrease in glucose absorption in
small intestine.
Thus, we conclude that this combination approach (LA + BCP) possesses the new
therapeutic regimen for the treatment of T2DM.

8. LIMITATIONS & PRECAUTIONS :
Limitations -
 Limited viability of intestine under in-
vitro conditions (about 2 hrs)
 Loss of enzymatic activity under in-
vitro conditions.
 Limited sampling points because of
viability constraints.
 Lack of nerve response through
neurons after drug exposure
Precautions -
 Temperature should be maintained at
37 ̊C.
 Do not touch the mucosal surface of the
intestine.
 No air bubbles should be present inside
the syringe or tubing.
 Knots should be tied firmly otherwise
deflation may occur.
 Continuous Aeration is necessary to keep
the intestine viable.
Source :- 1) Glucose transport into everted sacs of the small intestine of mice (Review paper)
2) Everted gut sac model as a tool in pharmaceutical research: limitations and applications (Review Paper)

9. APPLICATIONS :
 To investigate drug transport mechanism through intestine.
 To evaluate the rate and extent of absorption through different segments of
gastrointestinal tract.
 To identify the absorption window of drug molecules.
 To investigate drug–drug, and drug–food interaction.
 To investigate intestinal metabolism of drug molecules, e.g. enzyme degradation.
 To screen pharmaceutical excipients as well as formulations, for oral bioavailability
enhancement.
 To evaluate in-vitro mucoadhesive properties of oral bioadhesive microparticulate
formulations.
 To study the effect of disease state on absorption/expression of intestinal transporter.
 To investigate site of isomeric conversion.
 To study of intestinal conversion of prodrug into drug.
 To carry out drug permeation studies.
Source :- Everted gut sac model as a tool in pharmaceutical research: limitations and applications (Review Paper)

10. THANK YOU
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