This study analyzed glycogen storage and localization of glycogen metabolizing enzymes in the murine uterus from proestrus until implantation. Immunohistochemistry and western blot techniques were used to identify and locate key glycogen synthesizing enzymes in the glandular epithelium, luminal epithelium, and stroma. The results showed glycogen deposits and enzyme expression patterns in the different uterine tissues across the estrous cycle and implantation. The discussion agrees with previous studies that glucose levels must be carefully regulated for embryo development, and that glycogen may act as an energy reserve for preimplanted embryos.