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Hyperactivation the nitro boost Abhranil Das
Capacitation In the female reproductive tract or appropriate in vitro environment Requires several hours Principal processes ,[object Object]
Influx of extracellular calcium
Destabilization of sperm membrane in preparation for acrosomal reaction
Hyperactivation,[object Object]
How does it happen? The flagellum has ion channels formed by a protein called CatSper Selectively allows Ca2+ ion to enter Channels open, influx of Ca2+ ion The rise in calcium levels allows the tail to form deeper bends
How does it help? Greater propulsion through the viscous medium Enables escape from mucosal pockets in the oviduct Increases chance of finding the egg due to frequent direction changes Hyperactivated motility necessary for penetrating zonapellucida
Hyperactivated Bovine Sperm Hyperactivated sperm Normal sperm
Hyperactivated Bull Sperm A Normal bull sperm  BHyperactivatedsperm CMaximal form of hyperactivation Each image represents two flashes of a stroboscope, spaced 1/60 sec apart. Scale bar = 10 μm  c/o BiolReprod
Hyperactivated Hamster Sperm Normal Hyperactivated c/o Si Y, Okuno M BiolReprod Jul 1999
Morphology v/s Hyperactivation According to  Steven Green and Simon Fishel Morphology comparison of individually selected hyperactivated and non-hyperactivated human spermatozoa Human Reproduction  vol. 14  no. 1 1999
Morphology v/s Hyperactivation

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Hyperactivation

  • 1. Hyperactivation the nitro boost Abhranil Das
  • 2.
  • 4. Destabilization of sperm membrane in preparation for acrosomal reaction
  • 5.
  • 6. How does it happen? The flagellum has ion channels formed by a protein called CatSper Selectively allows Ca2+ ion to enter Channels open, influx of Ca2+ ion The rise in calcium levels allows the tail to form deeper bends
  • 7. How does it help? Greater propulsion through the viscous medium Enables escape from mucosal pockets in the oviduct Increases chance of finding the egg due to frequent direction changes Hyperactivated motility necessary for penetrating zonapellucida
  • 8. Hyperactivated Bovine Sperm Hyperactivated sperm Normal sperm
  • 9. Hyperactivated Bull Sperm A Normal bull sperm BHyperactivatedsperm CMaximal form of hyperactivation Each image represents two flashes of a stroboscope, spaced 1/60 sec apart. Scale bar = 10 μm c/o BiolReprod
  • 10. Hyperactivated Hamster Sperm Normal Hyperactivated c/o Si Y, Okuno M BiolReprod Jul 1999
  • 11. Morphology v/s Hyperactivation According to Steven Green and Simon Fishel Morphology comparison of individually selected hyperactivated and non-hyperactivated human spermatozoa Human Reproduction vol. 14 no. 1 1999
  • 14. Reference Papers Carmen R. Stauss, Timothy J Votta, Susan S Suarez Sperm Motility Hyperactivation Facilitates Penetration of the Hamster ZonaPellucida Biology of Reproduction 53 1999, 1280-1295 Steven Green and Simon Fishel Morphology comparison of individually selected hyperactivatedand non-hyperactivated human spermatozoa Human Reproduction vol. 14 no.1 1999 p. 123-130