The document discusses different types of genetic markers. It defines markers as genetic elements or DNA sequences that can be detected phenotypically. It classifies markers into molecular markers, biochemical markers, and DNA-based markers. It provides details on different types of DNA-based markers such as anonymous markers, defined markers, PCR-based markers, sequence tagged markers, and hybridization-based markers such as RFLPs. The document discusses the steps involved in RFLP analysis including DNA isolation, restriction digestion, electrophoresis, Southern blotting, hybridization, and autoradiography.
In nuclear biology and molecular biology, a marker gene is a gene used to determine if a nucleic acid sequence has been successfully inserted into an organism's DNA.
Molecular Marker and It's ApplicationsSuresh Antre
Molecular (DNA) markers are segments of DNA that can be detected through specific laboratory techniques. With the advent of marker-assisted selection (MAS), a new breeding tool is now available to make more accurate and useful selections in breeding populations.
Microsatellite are powerful DNA markers for quantifying genetic variations within & between populations of a species, also called as STR, SSR, VNTR. Tandemly repeated DNA sequences with the repeat/size of 1 – 6 bases repeated several times
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
In nuclear biology and molecular biology, a marker gene is a gene used to determine if a nucleic acid sequence has been successfully inserted into an organism's DNA.
Molecular Marker and It's ApplicationsSuresh Antre
Molecular (DNA) markers are segments of DNA that can be detected through specific laboratory techniques. With the advent of marker-assisted selection (MAS), a new breeding tool is now available to make more accurate and useful selections in breeding populations.
Microsatellite are powerful DNA markers for quantifying genetic variations within & between populations of a species, also called as STR, SSR, VNTR. Tandemly repeated DNA sequences with the repeat/size of 1 – 6 bases repeated several times
Marker Assisted Selection in Crop BreedingPawan Chauhan
Marker Assisted Selection is a value addition to conventional methods of Crop Breeding. It has been gaining importance in plant breeding with new generation of plant breeders and to get accurate and fast desired result from plant breeding.
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
All about molecular markers like - hybridization based markers (RFLP), pcr based markers (RAPD, SSR, SNP) etc. Morphological and biochemical markers also covered.
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
All about molecular markers like - hybridization based markers (RFLP), pcr based markers (RAPD, SSR, SNP) etc. Morphological and biochemical markers also covered.
Taxonomy is the branch of science concerned with the classification of organisms. A taxonomic designation is more than just a name. Ideally, it reflects evolutionary history and the relationship between organisms. Traditionally, taxonomic classification has relied upon morphological features and physiological characteristics. However, for bacterial taxonomy, phenotypic approaches have proven insufficient. Unrelated bacteria can exhibit identical traits, closely related bacteria can have divergent features, and methods for accurate identification may be too cumbersome for routine use. In contrast, molecular taxonomy approaches use data derived from hereditary material and provide a robust view of genetic relatedness. Advances in technology have been accompanied by improvements in the cost, speed, and availability of molecular methods. Here, we provide a brief history of approaches to prokaryotic classification and describe how molecular taxonomy is redefining our understanding of bacterial evolution and the tree of life.
Dr. S. MANIKANDAN, M.Sc., Ph.D
Lecturer in Botany
Thiruvalluvar University Model Constituent College,
Tittagudi 606 106, Tamil Nadu, India.
Email id: drgsmanikandan@gmail.com
Role of molecular marker play a significant supplementary role in enhancing yield along with conventional plant breeding methods. the result obtain through molecular method are more accurate and at genotypic level. It had wider applications in field of plant breeding, biotechnology, physiology, pathology, entamology, etc. The mapping information obtained from these markers had created a revolution in the sequencing sector and open many pathways for developments, innovations and research.
What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization
Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
Molecular markers for measuring genetic diversity Zohaib HUSSAIN
Molecular markers for measuring genetic diversity
Introduction:
The molecular basis of the essential biological phenomena in plants is crucial for the effective conservation, management, and efficient utilization of plant genetic resources (PGR).
Determining genetic diversity can be based on morphological, biochemical, and molecular types of information. However, molecular markers have advantages over other kinds, where they show genetic differences on a more detailed level without interferences from environmental factors, and where they involve techniques that provide fast results detailing genetic diversity
Comparison of different methods
Morphological characterization does not require expensive technology but large tracts of land are often required for these experiments, making it possibly more expensive than molecular assessment. These traits are often susceptible to phenotypic plasticity; conversely, this allows assessment of diversity in the presence of environmental variation.
Biochemical analysis is based on the separation of proteins into specific banding patterns. It is a fast method which requires only small amounts of biological material. However, only a limited number of enzymes are available and thus, the resolution of diversity is limited.
Molecular analyses comprise a large variety of DNA molecular markers, which can be employed for analysis of variation. Different markers have different genetic qualities (they can be dominant or co-dominant, can amplify anonymous or characterized loci, can contain expressed or non-expressed sequences, etc.).
Genetic marker
The concept of genetic markers is not a new one; in the nineteenth century, Gregor Mendel employed phenotype-based genetic markers in his experiments. Later, phenotype-based genetic markers for Drosophila melanogaster led to the founding of the theory of genetic linkage. A genetic marker is an easily identifiable piece of genetic material, usually DNA that can be used in the laboratory to tell apart cells, individuals, populations, or species. The use of genetic markers begins with extracting proteins or chemicals (for biochemical markers) or DNA (for molecular markers) from tissues of the plant (for example, seeds, foliage, pollen, sometimes woody tissues).
Molecular markers In genetics, a molecular marker (identified as genetic marker) is a fragment of DNA that is associated with a certain location within the genome. Molecular markers which detect variation at the DNA level such as nucleotide changes: deletion, duplication, inversion and/or insertion. Markers can exhibit two modes of inheritance, i.e. dominant/recessive or co-dominant. If the genetic pattern of homozygotes can be distinguished from that of heterozygotes, then a marker is said to be co-dominant. Generally co-dominant markers are more informative than the
Similar to Hybridization based molecular markers 1 (20)
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Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. Any GENETIC ELEMENT (GENE, DNA
SEQUENCE) which can be readily detected by
phenotype is marker. Also called Gene Tags.
According to Stansfield, the word marker is usually
used for “LOCUS MARKER”.
Barendse et al. 1994. Nature Genetics 6:227-235
5. These markers are the genes or
nucleotide sequence that has obvious
impact on morphology of plant or animal.
E.g. color, texture, height, disease
response, photoperiod sensitivity, fruits or
seeds and many other visually scorable
traits.
Classical markers which are also called
“naked eye polymorphism”.
Selected based on the experience of the
breeder to correlate a phenotypic trait with
a trait of interest.
Plant Omics Journal 2(4):141-162 (2009)
6. A sequence of DNA or protein that
can be screened to reveal key
attributes of its state or
composition and thus used to
reveal genetic variation.
In medicine a “molecular marker”
can be a substance that is
introduced in an organism as a
means to examine something.
Ex: Rubidium Chloride is used
as a radioactive isotope to
evaluate perfusion of heart
muscle.
Adams et al., 1992
7. Also known as “Genetic Marker”.
Genetic markers are the sequences of DNA which
have been traced to specific location on the
chromosomes and associated with particular
traits.
Molecular Markers are classified as:
1. Protein Based Markers/ Biochemical Markers
2. DNA Based Markers
8. Represent the actual
polymorphism of the
phenotype important for the
breeder.
Generally scored quickly,
simply and without
laboratory equipments.
Influenced by environment,
Time consuming, require
large population.
Represent naturally
occurring polymorphism in
DNA sequence (i.e. base
pair deletion, substitution,
addition or patterns)
Generally scored in
laboratory.
Comparatively less time
consuming and do not
require large population.
Adams et al., 1992
9. These are also called isozymes.
Allozymes = isozymes encoded by the different
alleles of the same gene, which is easily
visualized by activity of gel.
These are the electrophoretic variants of proteins
produced by different alleles at protein coding
genes, it also involve the detection of specific
metabolite usually based on color.
10. DNA marker = direct reflection of genotype.
“Any unique DNA sequence which can be used in
DNA hybridization, PCR or restriction mapping
experiments to identify that sequence.”
In 1980, variations in the pattern of DNA fragments
were observed generated by restriction enzyme
digestion of genomic DNA could be used as genetic
marker.
DNA-markers allow the breeder to introduce into
their cultivated plant only the gene(s) of interest
from a related species as compare to conventional
breeding.
Wakasugi et al., 1994a,b
11. Allow eliminating in a few generations ‘undesired’
genome regions.
Segregate as single genes.
Not affected by the environment.
DNA based markers are classified as:
1) Anonymous Markers
2) Defined Markers
Anonymous Markers:
“A cloned random DNA fragment whose
function or specific features are not known e.g.
Microsatellites and AFLP. These marker type
generally measure apparently neutral DNA
variations.
Wakasugi et al., 1994a,b
12. Defined Marker OR Polymorphic marker:
“A defined marker may contain a gene or some
other specific features, e.g. restriction sites for
cutting restriction enzymes, etc.”
Polymorphism of DNA Marker:
“DNA markers representing polymorphism in the
actual base sequence of DNA.”
This can be represented by:
Mutation at restriction site.
Insertion or deletion between restriction sites.
Mutations at single nucleotide.
Changes in number of repeat unit between
restriction site or PCR primer sites.
Wakasugi et al., 1994a,b
13. DNA Polymorphic markers are classified
into three categories:
1) PCR Based Markers
2) Sequence Tagged Sites
3) Hybridization Based Markers
PCR Based Markers:
“PCR based markers involve in vitro amplification
of particular DNA sequences or loci, with the help
of specifically and arbitrarily chosen primers and a
DNA polymerase enzyme. The amplified fragments
are separated electrophoretically and banding
patterns are detected by different methods i.e.
staining and autoradiography.”
14. Sequence Tagged Sites:
“Any sites on the genome that is
unambiguously defined in terms of flanking
primers that are used for PCR amplification
of this site are called sequence tagged
sites.” Polymorphism is based on the
number of times a simple sequence of
DNA, usually 2-3 base pairs, is repeated.
Tsumura et al., 1995
15. 1970s --- Genetic marker system based on DNA-DNA
Hybridization was developed.
Eukaryotes have large genome so genetic
polymorphism can’t easily be studied.
Hybridization technique reveal polymorphism over
there.
“The variation in the length of DNA fragments produced
by specific Restriction Endonucleases from genomic DNA
s of two or more individuals of a species is called
hybridization and markers produced by this technique are
called hybridization based molecular markers.”
Barendse et al. 1994. Nature Genetics 6:227-235
16. The type of hybridization based markers
includes
RFLP
Barendse et al. 1994. Nature Genetics 6:227-235
17. “RFLP is restriction fragment length
polymorphism, which are variations in the
DNA sequence of an individual which may be
detected by Restriction Endonucleases, which
cut the double stranded DNA whenever they
recognize a highly specific oligonucleotide
sequence or a restriction site.”
Amer J Bot 81:1309–1326
18.
19. “ A molecular method of genetic
analysis that allows individuals
to be identified on the basis of
unique patterns of restriction
enzymes cutting in specific
regions of DNA. It is an
application of Southern
Hybridization Procedure. ”
Amer J Bot 81:1309–1326
20. Steps of RFLP technique includes:
1) Isolation of DNA
2) Digestion of DNA by Restriction Endonucleases
3) Electrophoresis
4) Southern Blotting
5) Hybridization with Radioactive Probe
6) Autoradiography
Nucl Acids Res 18:6531–6535
22. Take DNA sample and add restriction enzymes
into it. It will produce DNA fragments of different
lengths.
Mol Genet Genomics 271:742–751
23. “Electrophoresis refers to the process of moving
negatively-charged molecules through the gel
with electricity. Shorter fragments move farther
away from their original location, while longer
ones stay closer. The segments aligned in parallel
rows. “
Mol Genet Genomics271:742–751
24. “Southern blotting is a method routinely used in
molecular biology for the detection of specific
DNA sequence in DNA samples. Southern blotting
combines’ transfer of electrophoresis separated
DNA fragments to a filter membrane and
subsequent fragment detection by hybridization
probes. ”
‘Nitrocellulose’ or ‘Nylon’ sheet is placed on top of
the gel.
Pressure is applied through paper towel.
Permanent attachment is done either by ‘Baking
(80ᴼC, 2 Hrs)’ or by ‘UV-exposure’.
Botstein et al.1980
25.
26. Membrane is then exposed to
“Hybridization Probe”.
Probe DNA is labeled so that it
can be detected easily.
Detection is usually done by
‘Radioactivity’ or tagging the
molecule with ‘Fluorescent
material’.
Winter & Kahl, 1995
27. “Autoradiography is an image on X-ray film left by
decay pattern of the radiations”
Treatment with radiations will produce an
autoradiograph with distinct colored parallel
bands on X-ray film.
Winter & Kahl, 1995
28.
29.
30. They are co- dominant.
Measure variation at the level of DNA sequence,
not protein sequence.
RFLP loci are very large so even very small
segments of chromosomes can be mapped and
also study phylogenetic relationship.
Very reliable for linkage analysis and for detecting
coupling phase of DNA molecules.
Plant Omics Journal 2(4):141-162 (2009)
31. Requires relatively very large amount of DNA.
Requirement of radioactive probe makes the
analysis expensive and hazardous.
They are not useful for detecting single base
change or point mutations.
It is time consuming, laborious, and expensive.
The level of polymorphism is low.
Plant Omics Journal 2(4):141-162 (2009)