Humanized antibodies are antibodies made from non-human species that have been modified to increase their similarity to human antibodies. They have progressed from murine antibodies to chimeric to humanized to fully human antibodies. Humanized antibodies can be prepared using transgenic mice or phage display technologies. Phage display involves inserting antibody genes into phages and selecting desirable proteins by screening against antigens. The selected proteins can then be produced and assembled into functional antibodies.

1. Humanized Antibody
Submitted to: Dr. Amber
Submitted by: Mahnoor Khawaja

2. Definition
◦ Humanized
antibodies are antibodies
made from non-human
species whose protein
sequences have been
modified to increase their
similarity to antibody
variants produced
naturally in humans.

3. Era of humanized antibody
Murine
Chimeric
Humanized
Human or Fully Human

5. Human or fully human
 A human antibody is one of which both
chain types, and the J chain in the case of
polymeric antibodies, are of human origin.

6. Preparation
 Strains used
Xeno
mouse
HuMab
Mouse
TC
Mouse

7. Technologies used
Phage
Display
Transgenic
Mice
Primarily
human
system

8. Transgenic mice

9. Phage display
• The genes for the V regions of antibody
heavy and light chains can be inserted
separately into these phages, and they
display the proteins on the surface.
 Gene isolated from;
(a) Naïve B-cells
(b) Immunized, antigen-specific memory B-
cells.

10. Selection
 The proteins may be selected by screening
against the antigen of choice, and the
desirable proteins may be produced and
secreted by bacteria infected by the
specific phage.
 The secreted proteins can be reassembled
in vitro to make functional antibodies.

11. Primarily human system
 The best approach so far for producing
antibody .
 Strategies used ;
Mouse myeloma cell line
transfected with human
immunoglobulin genes
Fusion of human B cells
with a mouse-human hybrid
'heteromyeloma'