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Fungi Propagation for DIY Domestic Mycologists
@Gil_Lopez, Cofounder Smiling Hogshead Ranch
Brooklyn Botanic Garden
Fall 2015
PRESENTATION OUTLINE
• Caution in Identification &
Harvesting
• Kingdoms
• Mushroom Anatomy & Lifecycle
• Three Classes of Fungi
• Cultivating Mushrooms at Home
• Cardboard Demo & Hands-on
• Supply List & Instructions
• Milk Crate Demonstration
THIS IS NOT A MUSHROOM
IDENTIFICATION CLASS
There are lots of ways to ID Mushrooms
including:
Smell it
Touch it
Make a Spore Print
Look for a Cup, a Ring or Warts
Look at the Shape of the Cap
Look at How the Gills Attach to the Cap
Look at the Shape of the Stem
Look at How the Stem Emerges from the Cap
Look at the Colors and Markings
Look Through a Microscope
SOME MUSHROOMS ARE TOXIC
Never eat any wild fungi you cannot ID 100% correctly
Honey Mushroom = Delicious Death Cap = Deadly
FIVE KINGDOM
CLASSIFICATION
Evidence strongly
suggests that animals &
fungi are sister groups
while plants evolutionary
lineage diverged over 1.1
billions years ago.
Fungi and Animals break
down and their own food
sources.
Animals and Fungi share
4 unique protein
sequences in their DNA.
MUSHROOM ANATOMY
Fungal Fruit & Mycelium
FruitingBody
Mycelium
Hyphae
Hyphae
Underground Hyphae
Stalk
Cap
Gills
MUSHROOM ANATOMY
Fungal Parts
Scale
Tubes
Pores
Stipe/Stalk
Scales
Cap
Gills
Ring
Volva
MUSHROOM LIFECYCLE
Sexual & Asexual Reproduction
TYPES OF FUNGI
Mutualists
Parasites
Saprophytes
MUTUALISTIC
Types of Fungi
Mycorrhizae grow on plant
roots, expanding surface area
and digesting nutrients. It can
also transfer photosynthesized
nutrients from plant to plant.
Lichens are a symbiotic relationship
between fungi and photosynthesizing
organisms (like algae).
PARASITIC
Types of Fungi
Corn Smut Athlete’s Foot
Cordyceps
SAPROTROPHYTIC
Types of Fungi
Mold on bread
Mushrooms on wood
Mushrooms on
coffee grinds
MAKING CARDBOARD
MUSHROOM SPAWN
Materials List:
Water
Scissors or knife
Corrugated cardboard
Mushroom stem butts
of: Oyster, Garden
Giants, Turkey Tails or
primary decomposers.
Small container with
fabric cover
http://www.mushroom-appreciation.com/mushroom-spawn-cardboard.html
MILK CRATE FUNGI PROPAGATION
Upcycling urban waste products to grow healthy mushrooms at home
SUPPLY LIST
Gloves & Rubbing alcohol
Starter Kit/Spawn
Spent Coffee Grinds
Sawdust/wood shavings
Milk Crate
Cardboard
Trash Bag
Knife
SUBSTRATE
INOCULATION
Gather materials
Sterilize tools
Prepare your
workstation
Mix the materials
SPAWN RUN
Put your inoculated substrate in an environment
with the recommended conditions for
colonization (e.g., 75° F forPleurotus ostreatus)
Allow the substrate to become fully colonized by
the mushroom mycelium. (called “the spawn
run”)
When the little mushrooms (called “primoidia”)
first appear (called pinning), put the substrate
with primoidia in an environment with the
recommended conditions to promote fruiting.
This will mean controlling temperature, light,
humidity, and air flow that your chosen species
likes.
Harvest your mushrooms! Ideally, just before
spores are released.

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Growing Edible Mushrooms Indoors

  • 1. Fungi Propagation for DIY Domestic Mycologists @Gil_Lopez, Cofounder Smiling Hogshead Ranch Brooklyn Botanic Garden Fall 2015
  • 2. PRESENTATION OUTLINE • Caution in Identification & Harvesting • Kingdoms • Mushroom Anatomy & Lifecycle • Three Classes of Fungi • Cultivating Mushrooms at Home • Cardboard Demo & Hands-on • Supply List & Instructions • Milk Crate Demonstration
  • 3. THIS IS NOT A MUSHROOM IDENTIFICATION CLASS There are lots of ways to ID Mushrooms including: Smell it Touch it Make a Spore Print Look for a Cup, a Ring or Warts Look at the Shape of the Cap Look at How the Gills Attach to the Cap Look at the Shape of the Stem Look at How the Stem Emerges from the Cap Look at the Colors and Markings Look Through a Microscope
  • 4. SOME MUSHROOMS ARE TOXIC Never eat any wild fungi you cannot ID 100% correctly Honey Mushroom = Delicious Death Cap = Deadly
  • 5. FIVE KINGDOM CLASSIFICATION Evidence strongly suggests that animals & fungi are sister groups while plants evolutionary lineage diverged over 1.1 billions years ago. Fungi and Animals break down and their own food sources. Animals and Fungi share 4 unique protein sequences in their DNA.
  • 6. MUSHROOM ANATOMY Fungal Fruit & Mycelium FruitingBody Mycelium Hyphae Hyphae Underground Hyphae Stalk Cap Gills
  • 8. MUSHROOM LIFECYCLE Sexual & Asexual Reproduction
  • 10. MUTUALISTIC Types of Fungi Mycorrhizae grow on plant roots, expanding surface area and digesting nutrients. It can also transfer photosynthesized nutrients from plant to plant. Lichens are a symbiotic relationship between fungi and photosynthesizing organisms (like algae).
  • 11. PARASITIC Types of Fungi Corn Smut Athlete’s Foot Cordyceps
  • 12. SAPROTROPHYTIC Types of Fungi Mold on bread Mushrooms on wood Mushrooms on coffee grinds
  • 13. MAKING CARDBOARD MUSHROOM SPAWN Materials List: Water Scissors or knife Corrugated cardboard Mushroom stem butts of: Oyster, Garden Giants, Turkey Tails or primary decomposers. Small container with fabric cover http://www.mushroom-appreciation.com/mushroom-spawn-cardboard.html
  • 14. MILK CRATE FUNGI PROPAGATION Upcycling urban waste products to grow healthy mushrooms at home
  • 15. SUPPLY LIST Gloves & Rubbing alcohol Starter Kit/Spawn Spent Coffee Grinds Sawdust/wood shavings Milk Crate Cardboard Trash Bag Knife
  • 17. SPAWN RUN Put your inoculated substrate in an environment with the recommended conditions for colonization (e.g., 75° F forPleurotus ostreatus) Allow the substrate to become fully colonized by the mushroom mycelium. (called “the spawn run”) When the little mushrooms (called “primoidia”) first appear (called pinning), put the substrate with primoidia in an environment with the recommended conditions to promote fruiting. This will mean controlling temperature, light, humidity, and air flow that your chosen species likes. Harvest your mushrooms! Ideally, just before spores are released.