The document discusses research on producing valuable compounds through plant tissue culture and fungal fermentation. Key points discussed include:
1) Production of anticancer compounds like camptothecin from tissue cultures of plants like Chonemorpha grandiflora.
2) Metabolic engineering of plant tissues through gene overexpression to enhance production of compounds, as shown with tropane alkaloid production in transgenic Scopolia parviflora hairy roots.
3) Optimization of nutrient media and growth conditions to maximize biosynthesis of commercially important compounds in plant tissues and fungi, such as for taxanes in Taxus and galanthamine in Leucojum aestivum shoot cultures.
Role of Pharmacognosy in various systems of medicineRamaiah Maddi
Role of Pharmacognosy in Allopathy (Modern medicine)
Role of Pharmacognosy in Ayurveda
Role of Pharmacognosy in Unani System of Medicine
Role of Pharmacognosy in Siddha System of Medicine
Role of Pharmacognosy in Homeopathy
Role of Pharmacognosy in Chinese Medicine System
This practical manual is prepared for academic purpose only and contains introduction and detail methodology regarding determination of numbers of starch grains by Lycopodium spore method, along with dummy readings to facilitate understanding of calculations thereof.
Polyploidy, mutation and hybridization with reference to medicinal plants. PH...Ms. Pooja Bhandare
Polyploidy, mutation and hybridization with reference to medicinal plants. PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-4
Polyploidy reference to medicinal plants.
Types Of Polyploidy
A. Euploidy
a.Autopolyploidy
b. Allopolyploidy
B. Aneuploidy
1. Causes Of Polyploidy
2. Non-disjunction in mitosis
3. Non-reduction in meiosis
4. Polyspermy
5. Endo-replication or Endo- reduplication.
Factors Promoting Polyploidy
1. Physical factor
2. Chemical factor
3. Biological factor
Physical factor:-
Temperature :- heat temperature & cold temperature
Centrifugation
X-rays
Gamma rays
Cosmic rays
Ionizing & non-ionizing radiations
UV-radiations
Chemical factor:-
Alkylating agents:- nitrogen & sulphur mustard
Acridines
Proflavins
Nitrous acid
Colchicines[6]
Colchicines (Poisonous alkaloids):-
Biological factor
Mode of reproduction
Mode of fertilization
Breeding system present (Hybridization)
Growth habit of the plant
Size of chromosomes
Application Of Polyploidy
Mutation breeding
Seedless fruits production
Bridge crossing
Ornamental & forage breeding
Disease resistance through aneuploidy
Industrial application of polyploidy
mutation reference to medicinal plants
Type of mutations:
1. Spontaneous and induced mutations.
2. Recessive and dominant mutations.
3. Somatic and germinal mutations.
4. Forward, back and suppressor mutation.
5. Chromosomal, genomic and point mutations
Application Of Mutation:
Hybridization reference to medicinal plants
The following steps are involved in hybridization of plant:
Choice Of Parents:.
Selfing Of Parents
Emasculation:.
Bagging:
Crossing Or Cross Pollination
Labelling
Collection Of Hybrid Seeds
Significance of Hybridization
Role of Pharmacognosy in various systems of medicineRamaiah Maddi
Role of Pharmacognosy in Allopathy (Modern medicine)
Role of Pharmacognosy in Ayurveda
Role of Pharmacognosy in Unani System of Medicine
Role of Pharmacognosy in Siddha System of Medicine
Role of Pharmacognosy in Homeopathy
Role of Pharmacognosy in Chinese Medicine System
This practical manual is prepared for academic purpose only and contains introduction and detail methodology regarding determination of numbers of starch grains by Lycopodium spore method, along with dummy readings to facilitate understanding of calculations thereof.
Polyploidy, mutation and hybridization with reference to medicinal plants. PH...Ms. Pooja Bhandare
Polyploidy, mutation and hybridization with reference to medicinal plants. PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-4
Polyploidy reference to medicinal plants.
Types Of Polyploidy
A. Euploidy
a.Autopolyploidy
b. Allopolyploidy
B. Aneuploidy
1. Causes Of Polyploidy
2. Non-disjunction in mitosis
3. Non-reduction in meiosis
4. Polyspermy
5. Endo-replication or Endo- reduplication.
Factors Promoting Polyploidy
1. Physical factor
2. Chemical factor
3. Biological factor
Physical factor:-
Temperature :- heat temperature & cold temperature
Centrifugation
X-rays
Gamma rays
Cosmic rays
Ionizing & non-ionizing radiations
UV-radiations
Chemical factor:-
Alkylating agents:- nitrogen & sulphur mustard
Acridines
Proflavins
Nitrous acid
Colchicines[6]
Colchicines (Poisonous alkaloids):-
Biological factor
Mode of reproduction
Mode of fertilization
Breeding system present (Hybridization)
Growth habit of the plant
Size of chromosomes
Application Of Polyploidy
Mutation breeding
Seedless fruits production
Bridge crossing
Ornamental & forage breeding
Disease resistance through aneuploidy
Industrial application of polyploidy
mutation reference to medicinal plants
Type of mutations:
1. Spontaneous and induced mutations.
2. Recessive and dominant mutations.
3. Somatic and germinal mutations.
4. Forward, back and suppressor mutation.
5. Chromosomal, genomic and point mutations
Application Of Mutation:
Hybridization reference to medicinal plants
The following steps are involved in hybridization of plant:
Choice Of Parents:.
Selfing Of Parents
Emasculation:.
Bagging:
Crossing Or Cross Pollination
Labelling
Collection Of Hybrid Seeds
Significance of Hybridization
GPAT
Niper &
other pharma competative exams...
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a complete description on medically important plant taxus Wallachiana
its transverse section , microscopic characters, macroscopic characters,
and its medical uses
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
Synonyms:- Vinca rosea, Catharanthus, Madagascar,periwinkle.
Barmasi.
Biological Source:- Vinca is the dried entire plant of Catharanthus roseus Linn .belonging to family Apocynaceae
Geographical Source:- The plant is a native of Madagascar and is found in manytropical and subtropical countries especially in India, Australia,South Africa and North and South America. The plant is cultivated as garden plant in Europe and India.
Cloves are the aromatic flower buds of a tree in the family Myrtaceae, Syzygium aromaticum. They are native to the Maluku Islands in Indonesia, and are commonly used as a spice. Cloves are commercially harvested primarily in Indonesia, India, Madagascar, Zanzibar, Pakistan, Sri Lanka and Tanzania.
Pharmacognosy of Rauwolfia serpentina, biological source, geographical source, marphology of roots and rhizome, microscopy of roots, chemical constituents- reserpine, uses -antihypertensive, isolation of reserpine, serpagandha, India snake root
Cultivation and collection of drugs of natural originZuli Shingala
cultivation and collection of medicinal plants, processing, storage, etc. with various factors that affects cultivation and collection of medicinal plants
Unit II Introduction to secondary metabolite
Phenylpropanoids and Flavonoids: Lignans, Tea, Ruta
For video lecture join to youtube channel snehal chakorkar
Secondary metabolites from plants like Alkaloids, Glycosides, Tannins, Resins, Volatile oils and Flavonoids. with definition, introduction, Chemical properties, Classification and Identification test
GPAT
Niper &
other pharma competative exams...
For more posts, follow us on .... be
@pharmahelpers
@pharmahelpers
@pharmahelpers
Also follow us on...
Facebook @ Pharma Helpers
Whatsapp @ 8421773854 OR https://chat.whatsapp.com/FFVRcxiiqC91PvnEvmK2F1
Instagram @ https://www.instagram.com/p/COM-VYqJ3Xo/?igshid=1ac01ibq2sa2k
a complete description on medically important plant taxus Wallachiana
its transverse section , microscopic characters, macroscopic characters,
and its medical uses
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
Synonyms:- Vinca rosea, Catharanthus, Madagascar,periwinkle.
Barmasi.
Biological Source:- Vinca is the dried entire plant of Catharanthus roseus Linn .belonging to family Apocynaceae
Geographical Source:- The plant is a native of Madagascar and is found in manytropical and subtropical countries especially in India, Australia,South Africa and North and South America. The plant is cultivated as garden plant in Europe and India.
Cloves are the aromatic flower buds of a tree in the family Myrtaceae, Syzygium aromaticum. They are native to the Maluku Islands in Indonesia, and are commonly used as a spice. Cloves are commercially harvested primarily in Indonesia, India, Madagascar, Zanzibar, Pakistan, Sri Lanka and Tanzania.
Pharmacognosy of Rauwolfia serpentina, biological source, geographical source, marphology of roots and rhizome, microscopy of roots, chemical constituents- reserpine, uses -antihypertensive, isolation of reserpine, serpagandha, India snake root
Cultivation and collection of drugs of natural originZuli Shingala
cultivation and collection of medicinal plants, processing, storage, etc. with various factors that affects cultivation and collection of medicinal plants
Unit II Introduction to secondary metabolite
Phenylpropanoids and Flavonoids: Lignans, Tea, Ruta
For video lecture join to youtube channel snehal chakorkar
Secondary metabolites from plants like Alkaloids, Glycosides, Tannins, Resins, Volatile oils and Flavonoids. with definition, introduction, Chemical properties, Classification and Identification test
In vitro mutagenesis of Cymbidium La bell “Anna Belle” by γ-rays irradiation ...IJEAB
The optimum media for multiplication of protocorm like bodies (PLBs) and shoot buds of Cymbidium La bell “Anna Belle” were studied in order to prepare the in vitro samples for irradiation. The values of LD50 (lethal dose of 50% samples) of PLBs, shoot buds and plantlets of tested Cymbidium after cultivation of 4 months were also determined about 35.0, 41.0 and 83.1 Gy, respectively. The addition of oligochitosan played as an very important trigger for promotion on the generation of shoot bud from PLBs after irradiation. The in vitro variations have been generated by γ-rays irradiation of PLBs with doses in range of 20 - 50 Gy. The highest mutant frequency (3.83‰) of C. La bell was found by the irradiation of PLB samples at 30 Gy. The different properties of obtained in vitro variations compared to wild types were found to be chlorophyll, short leaves, long leaves, and violet pericardium variations. The genetic relationships among generated variant lines in M1V4 and wild type were analyzed using RAPD techniques.
Growth Pattern, Molecular Identification and Bio molecules Analysis of FOMITO...journal ijrtem
Abstract : Fomitopsis feei, a brown rot fungus is identified tentatively using morphological characteristics and confirmed phylogenetically by 28S rDNA analysis and sequence was submitted in EMBL Nucleotide Sequence Database. Its growth pattern was studied on eight different solid media and found to be good on Malt extract agar medium. Biomolecules such as proteins and lipid were screened qualitatively and estimated quantitatively. Aminoacid analysis by chromatography and fatty acid analysis by FAME were also done and revealed that tryptophan (20.53%), valine (20.51%) and cis-linoleic acid (43.38%) and palmetic acid (17.88%) were in high percentage.
Key words : Fomitopsis feei, growth, molecular identification and biomolecules
DNA construct instability in bacteria used for Agrobacterium mediated plant t...iosrjce
The use of plasmid in the production of genetically modified (GM) crops is highly essential in
research and in commercial production of GM plants. However plasmid instability constitutes a major problem
in the use of recombined microorganisms in the production of GM crops. In this study we evaluated the stability
of p8114 carrying a gene coding for a transcription factor (TFIIIA) driven by Cassava Vein Mosaic Virus
(CsVMV) promoter and an nptII selectable marker driven by 35S promoter in the T-DNA. The plasmid was
amplified in E.coliDH5α strain on Luria Broth (LB)agar supplemented with 100 µg/ml kanamycin. The colonies
were confirmed by Restriction Fragment Length Analysis (RFLA) and by DNA sequencing. The confirmed
colonies were stored as glycerol stock at -80
0C and as DNA extracts in TE buffer at 40C. Agrobacterium strains
LBA4404, EHA 105 and AGL1 were also transformed with DNA from the confirmed colonies. Plasmid stability
was evaluated after 3 months. Sixteen to hundred percent level of instability was observed in E.colicolonies
stored at -80
0C and 50% level of instability in plasmid transformed into Agrobacterium strain LBA4404.
Agrobacterium strain LBA4404 showed a higher level of stability 75% compared to EHA 105 (0%) and AGL1 (50%).
COMPARATIVE STUDY OF CAPSAICIN FROM IN VITRO CULTIVATED AND NATURALLY CULTIVA...Dr Dama
COMPARATIVE STUDY OF CAPSAICIN FROM IN VITRO CULTIVATED AND NATURALLY CULTIVATED CAPSICUM FRUITS EXTRACTS
*Vinchurkar A.S., *Sonawane S. R., *Sherkhane S.S., *Mane P. P., *Valsange A.B. and *Dama L. B.
Comparative Cytotoxic Activities of the Flavonoid-Rich Ethyl Acetate Fruit Ex...inventionjournals
The fruit of Pouteriacampechianahas been previously reported to contain flavonoids and polyphenolic substances with high in vitro anti-oxidative effects. Its anti-tumorigenic activities have not been previously demonstrated. This study seeks to demonstrate the cytotoxic effects of the flavonoid-rich ethyl acetate fraction of the fruit extract of P. campechiana(EAFFPC) against K562 leukemic cell lines and healthy human whole blood cells. The standardized MTT-dye cell viability assay was carried out to measure the cytotoxicity of EAFFPC against the aforementioned cell lines cultured in RPMI. The assay showed that at a 60 µg well concentration, EAFFPC exhibited a 55.4% cell viability which is significantly lower than the cell viability obtained with 10 µg of vincristine. In contrast, EAFFPC demonstrated concentration-dependent cytoprotective properties in healthy human whole blood cells (HHWBC). This study confirms specific nonconcentration-dependent cytotoxic effects on K562 leukemic cell lines while exhibiting a concentrationdependent cytoprotective effects in HHWBC
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Factory Supply Best Quality Pmk Oil CAS 28578–16–7 PMK Powder in Stockrebeccabio
Factory Supply Best Quality Pmk Oil CAS 28578–16–7 PMK Powder in Stock
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Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
How to Give Better Lectures: Some Tips for Doctors
Applications in plant tissue culture
1.
2. (1) Extracts from black carrot tissue culture as
potent anticancer agents.
(2) Enhanced production of tropane alkaloids in
transgenic Scopolia parviflora hairy root cultures
over-expressing putrescine N-methyl transferase
(PMT) and hyoscyamine-6β-hydroxylase (H6H).
(3) Taxus globosa S. cell lines: initiation,
selection and characterization in terms of
growth, and of baccatin III and paclitaxel
production.
(4) Production of camptothecin in cultures of
Chonemorpha grandiflora.
3. APPLICATION IN PLANT TISSUE CULTURE (CONT.,)
(5) Regeneration, in vitro glycoalkaloids
production and evaluation of bioactivity of callus
methanolic extract of Solanum tuberosum L.
(6) The influence of medium composition on
alkaloid biosynthesis by Penicillium citrinum.
(7) Differential production of tropane alkaloids in
hairy roots and in vitro cultured two accessions
of Atropa belladonna L. under nitrate treatments.
(8) Increased vincristine production from
Agrobacterium tumefaciens C58 induced shooty
teratomas of Catharanthus roseus G. Don.
4. APPLICATION IN PLANT TISSUE CULTURE (CONT.,)
(9) Enhancement of taxane production in hairy
root culture of Taxus x media var. Hicksii
(10) An endophytic taxol-producing fungus from
Taxus media, Cladosporium cladosporioides MD2.
(11) Optimized nutrient medium for galanthamine
production in Leucojum aestivum L. in vitro shoot
system.
(12) Salinity stress enhances production of
solasodine in Solanum nigrum L.
5. APPLICATION IN PLANT TISSUE CULTURE (CONT.,)
(13) Biosynthesis of hyperforin and
adhyperforin from amino acid precursors in
shoot cultures of Hypericum perforatum.
7. Black carrots contain anthocyanins.
Explants of young black carrot shoots were
cultured in Murashige and Skoog (MS)
medium for callus initiation and were
transferred to new MS medium
supplemented with four different
combinations of 2,4-dichlorophenoxyacetic
acid and kinetin.
Subsequently, the lyophilized calli and
black carrot harvested from fields were
subjected to ultrasound extraction with
ethanol at a ratio of 1:15 (w:v).
8. Extracts applied to
(human breast adenocarcinomas), (human
colon adenocarcinoma), (human
prostate adenocarcinoma), Neuro 2A
(Musmusculus neuroblastoma) cancer cell lines
and VERO (African green monkey kidney) normal
cell line by MTT assay.
The highest cytotoxic activity was achieved
against Neuro-2A cell lines exhibiting viability of
38-46% at 6.25 μg/ml concentration for all calli
and natural extracts. However, a significantly
high IC50 value of 170.13 μg/ml was attained in
normal cell line VERO indicating that its natural
counterpart is an ideal candidate for treatment
of brain cancer without causing negative effects
to normal healthy cells.
10. Scopolia parviflora adventitious roots were
metabolically engineered by co-expression of
the two gene putrescine N-methyl
transferase (PMT) and hyoscyamine-6β-
hydroxylase (H6H) cDNAs with the aid of
Agrobacterium rhizogenes.
The transformed roots developed into
morphologically distinct S. parviflora PMT1
(Sp PMT1), S. parviflora PMT2 (Sp PMT2), and
S. parviflora H6H (Sp H6H) transgenic hairy
root lines.
Consequent to the introduction of these key
enzyme genes, the production of the
alkaloids hyoscyamine and scopolamine was
enhanced.
11. Among the transgenic hairy root lines, Sp
PMT2 line possessed the highest growth
index.
The treatment of transgenic hairy roots
with growth regulators further enhanced
the production of scopolamine.
Thus, the results suggest that PMT1, PMT
2, and H6H genes may not only be involved
in the metabolic regulation of alkaloid
production but also that these genes may
play a role in the root development.
13. Of the initial six cell lines originating from
explants of Taxus globosa, or Mexican yew
(stem internode,leaves,meristematic tissue)
Three were selected for their microbial and
oxidation resistance, two from leaves and
the other from stem internode
A study of their behavior, both in terms of
cell growth, and of baccatin III and paclitaxel
production, was developed in suspension
cultures with an initially standardized
biomass (fresh weight 0.23 g/L) using
modified Gamborg's B5 medium, and an
elicitor (methyl jasmonate), on either the
first or seventh day of culture, at several
levels (0, 0.1, 1, 10, 100 microM).
14. In most of the conditions used, the three
cell lines showed growth associated
baccatin III production.
The cell line from stem internode was the
highest producer of baccatin III using 1
microM elicitor, sampling at 10 days (p < or
= 0.01, 6.45 mg/L).
This same line also had the highest biomass
production (6.85 g/L, p < or = 0.01) at 10
days of culture but at the higher elicitor
concentration of 10 microM.
All three cell lines did not produce
paclitaxel under experimental conditions
used.
16. METHODS:
Callus cultures of C. grandiflora were
raised on Murashige and Skoog's medium
supplemented with 2, 4-D.
Stem with bark and callus were used for
phytochemical analysis mainly the
alkaloids.
Detection and identification of
camptothecin was carried out using
thin-layer chromatography (TLC), high-
performance thin-layer chromatography,
(HPTLC) and high-performance liquid
chromatography (HPLC).
17. RESULTS:
An important anticancer alkaloid,
camptothecin was detected in ethanolic
extracts of stem with bark and callus
cultures of C. grandiflora. camptothecin
content was 0.013 mg/g in stem with bark
and 0.003 mg/g in callus.
CONCLUSION:
This is the first report on in vivo and in vitro
production of camptothecin in C. grandiflora.
Camptothecin is known to occur only in
six plant sources so, alternative sources for
camptothecin are needed. Thus of C.
grandiflora could be anew promising
alternative source of camptothecin
19. Callus and differentiated shoots initiated
from Solanum tuberosum L. on MS media
containing BA, IAA, and Kin. Glycoalkaloids
are produced in callus and shoots in
concentrations higher than original tubers
using HPLC.
Callus methanolic extract had promising
anticancer activity with low IC(50) values
against human carcinoma cell lines of
breast, lymphoplastic leukemia, larynx,
liver, cervix, colon, and brain.
IC(50) (microg/mL) were 2.7, 3.7, 6, 6.7,
10, 13.6, and 22.3 respectively.
20. Antioxidant capacity of the extract (76.4%)
performed using ESR. Preliminary screening
showed that the extract exhibited in vitro
virucidal activity against Herpes simplex.
The extract possessed in vitro
schistomicidal and fasciolicidal activity.
22. The fungus P. citrinum produces secondary
metabolites, clavine ergot alkaloids (EA), and
quinoline alkaloids quinocitrinines (QA) in
medium with various carbon and nitrogen
sources and in the presence of iron, copper,
and zinc additives.
Mannitol and sucrose are most favorable for
EA biosynthesis and mannitol is most
favorable for QA.
Maximum alkaloid production is observed on
urea.
23. Iron and copper additives in the medium
containing zinc ions stimulated fungal growth
but inhibited alkaloid biosynthesis.
The production of these secondary
metabolites does not depend on the
physiological state of culture, probably due
to the constitutive nature of the enzymes
involved in biosynthesis of these substances
25. Effects of nitrate concentrations (KNO3) on
the production of two tropane alkaloids,
hyoscyamine and scopolamine.
The growth of aerial parts and roots of two
in vitro propagated accessions of Atropa
belladonna and hairy roots were studied .
As hairy roots cultures are able to keep a
stable production of alkaloids over long
periods of subculturing, they are considered
as an interesting option for the study of
alkaloid biosynthesis.
A hairy roots culture of Atropa belladonna
was established by transformation with
Agrobacterium rhizogenes strain AR15834.
26. The results of our study showed that a rise in
KNO3 concentration caused a decline in hairy
roots growth, and had a remarkable effect on
the alkaloid content.
The alkaloid concentrations obtained in the
hairy roots were 3-20 times higher than that
in the plants at 35 mM of KNO3.
Increasing the nitrate concentration in the
medium of hairy roots also improved the
hyoscyamine/scopolamine ratio, while it
increased the scopolamine/hyoscyamine
ratio in the studied plants
28. Dimeric indole alkaloids are used extensively
for cancer therapy.
Agrobacterium tumefaciens C58 strain was
used for induction of shooty teratoma in
Catharanthus roseus using epicotyl and stem
node explants.
The transformed nature of shooty teratomas
was confirmed by nopaline assay.
Growth kinetics of shooty teratomas depicted
maximum growth during 21-24 days of culture.
Dimeric alkaloid vincristine in the transformed
cultures was present at a concentration of
0.011 that was tenfold higher compared to
untransformed control cultures
29. Baccatin III
J Plant Physiol. 2009 Nov 15;166(17):1950-4. doi: 10.1016/j.jplph.2009.05.001. Epub 2009 Jul 1.
30. This study assessed the effect of two
precursors (l-phenylalanine and p-amino
benzoic acid) used alone or in combination
with methyl jasmonate, on the growth and
accumulation of paclitaxel, baccatin III and
10-deacetylbaccatin III in hairy root cultures
of Taxus x media var. Hicksii.
The greatest increase in dry biomass was
observed after 4 weeks of culturing hairy
roots in medium supplemented with 1microM
of l-phenylalanine (6.2gL(-1)).
Addition of 1microM of l-phenylalanine to
the medium also resulted in the greatest 10-
deacetylbaccatin III accumulation
(422.7microg L(-1)), which was not detected
in the untreated control culture.
31. Supplementation with 100microM of l-
phenylalanine together with 100microM of
methyl jasmonate resulted in the enhancement
of paclitaxel production from 40.3microg L(-1)
(control untreated culture) to 568.2microg L(-
1), the highest paclitaxel content detected in
the study.
The effect of p-amino benzoic acid on taxane
production was less pronounced, and the
highest yield of paclitaxel (221.8microg L(-1))
was observed when the medium was
supplemented with 100microM of the precursor
in combination with methyl jasmonate.
Baccatin III was not detected under the
conditions used in this experiment and the
investigated taxanes were not excreted into
the medium
33. Fermentation processes using taxol-
producing fungi other than Taxus spp. may
be an alternative way to produce taxol,
which is an important antitumor agent used
widely in the clinic setting.
In this study, a taxol-producing endophytic
fungus strain MD2 was isolated from the
inner bark of Taxus media.
Strain MD2 produced taxol when grown in
potato dextrose liquid medium.
The fungal taxol-which was analyzed by
ultraviolet, high-performance liquid
chromatography and mass spectrometry-was
shown to be identical to authentic taxol and
10-deacetylbaccatin III.
34. Further analysis with nuclear magnetic
resonance (NMR) spectroscopy to show the
chemical structure of the fungal taxol
indicated that the fungal taxol produced an
NMR spectrum identical to that of authentic
taxol.
Strain MD2 was identified as Cladosporium
cladosporioides according to morphology of
the fungal culture, characteristics of the
spores, and analysis of 18S rDNA sequence.
In addition, 10-deacetylbaccatin III-10-O-
acetyl transferase gene of C. cladosporioides
MD2 was cloned for the first time and was
shown to share 99% identity with that of T. x
media and 97% identity with that of T.
wallichiana var. mairei.
36. The common effect of NH4+, NO3-, KH2PO4
and sucrose on the biosynthesis of
galanthamine by a Leucojum aestivum
shootculture was studied.
Polynominal regression models were
elaborated for the description of the
galanthamine biosynthesis as a
consequence of variation of the
investigated variables (NH4+ between 0.20
and 0.54 g/L; NO3- between 1.44 and 3.44
g/L; KH2PO4 between 0.10 and 0.24 g/L,
and sucrose between 30.00 and 60.00 g/L).
37. Optimization procedures allowed us to
establish the optimal concentrations of the
investigated variables and to propose the
modified MS nutrient medium, with 4.50
g/L KNO3, 0.89 g/L NH4NO3, 1.25 g/L
(NH4)2SO4, 0.10 g/L KH2PO4 and 60 g/L
sucrose, for the galanthamine production
by a Leucojum aestivum shoot culture.
The proposed modified MS medium
provided considerable increase of both the
production yield and the relative content of
the target alkaloid in the alkaloid mixture.
39. Various in vitro grown tissues (non-
regenerative callus, regenerative callus and
microshoot derived leaves) of Solanum
nigrum L. were cultured under salinity
stress (0-150 mM NaCl) for enhanced
production of solasodine, a steroidal
alkaloid and an alternative to diosgenin,
which is used as a precursor for the
commercial production of steroidal drugs.
The role of plant growth regulators and
various concentrations of NaCl during in
vitro production of solasodine was studied.
The in vitro yield was compared with the
yield from leaves of field grown plant.
40. Solasodine content was maximum (2.39 mg/g
dry wt.) in regenerative callus when grown
on medium added with 150 mM NaCl;
followed by in vitro raised leaf of
microshoot.
Quantitative estimation of solasodine was
carried out using a new HPTLC method,
which is validated for its recovery and
precession.
The proposed HPTLC method showed a good
linear relationship (r(2)=0.994) in 50-2000
ng/spot concentration ranges.
The data demonstrate that the solasodine
production in cultures was growth dependent
42. Hyperforin and adhyperforin contribute to
the antidepressant effects of Hypericum
perforatum.
The involvement of branched-chain amino
acids in the biosynthesis of hyperforin and
adhyperforin was demonstrated in H.
perforatum shoot cultures.
L-[U-(13)C(5)]Valine and L-[U-
(13)C(6)]isoleucine, upon administration to
the shoot cultures, were incorporated into
acyl side chain of hyperforin and
adhyperforin, respectively.
43. Feeding the shoot cultures with unlabelled
L-isoleucine at a concentration of 2mM
induced a 3.7-fold increase in the
production of adhyperforin.
The addition of 3mM L-threonine, a
precursor of isoleucine, stimulated a 2.0-
fold increase in the accumulation of
adhyperforin.
The administration of L-valine at
concentrations of 0-5mM had no stimulating
effect on the hyperforin production in H.
perforatum shoot cultures.
44. (1) Sevimli-Gur C, Cetin B, Akay S, Gulce-Iz S, Yesil-Celiktas o.
Plant Foods Hum Nutr. 2013 Sep;68(3):293-8. doi:
10.1007/s11130-013-0371-z.
(2) Kang, Y.; Park, D.; Min, J.; Song, H.; Jeong, M.; Kim, Y.;
Kang, S.; Karigar, C.; Choi, M. In Vitro Cellular & Developmental
Biology Plant . Aug2011, 47(4), p516-524. 9p.
(3) Barradas-Dermitz DM, Hayward-Jones PM, Mata-Rosas M,
Palmeros-Sánchez B, Platas-Barradas OB, Velásquez-Toledo RF.
Biocell. 2010;34:1–6.
(4) Kulkarni AV, Patwardhan AA, Lele U, Malpathak NP.
Pharmacognosy Res. 2010;2:296–9.
45. (5) Al-Ashaal HA. Fitoterapia. 2010;81(6):600–6.
(6) Koslovskiĭ AG, Zhelifonova VP, Antipova TV, Zelenkova
NF. Prikl Biokhim Mikrobiol. 2010;46(5):572–6.
(7) Chashmi NA, Sharifi M, Karimi F, Rahnama H.
Z Naturforsch C. 2010;65(5-6):373–9.
(8) Begum F, Nageswara Rao SS, Rao K, Prameela Devi Y,
Giri A, Giri CC. Nat Prod Res. 2009;23(11):973–81.
46. (9) Syklowska-Baranek K, Pietrosiuk A, Kokoszka A,
Furmanowa M. J Plant Physiol. 2009;166(17):1950–4.
(10) Zhang P, Zhou PP, Yu LJ. Curr Microbiol. 2009
Sep;59(3):227-32.
(11) Georgiev V, Berkov S, Georgiev M, Burrus M,
Codina C, Bastida J, Z Naturforsch C.2009;64(3-4):219–
24.
(12) Bhat MA, Ahmad S, Aslam J, Mujib A,
Chem Pharm Bull (Tokyo) 2008;56(1):17–21.
(13) Karppinen K, Hokkanen J, Tolonen A, Mattila
S, Hohtola A. Phytochemistry. 2007 Apr;68(7):1038-45.