As a component of the endocrine system, both male and female gonads produce sex hormones. Male and female sex hormones are steroid hormones and as such, can pass through the cell membrane of their target cells to influence gene expression within cells. Gonadal hormone production is regulated by hormones secreted by the anterior pituitary in the brain. Hormones that stimulate the gonads to produce sex hormones are known as gonadotropins. The pituitary secretes the gonadotropins luteinizing hormone (LH) and follicle-stimulating hormone (FSH). These protein hormones influence reproductive organs in various ways. LH stimulates the testes to secrete the sex hormone testosterone and the ovaries to secrete progesterone and estrogens. FSH aids in the maturation of ovarian follicles (sacs containing ova) in females and sperm production in males.
Steroid hormones can be grouped into 2 classes, corticosteroids (typically made in the adrenal cortex, hence cortico-) and sex steroids (typically made in the gonads or placenta).
Steroid Hormones - Dr. P. Saranraj, Assistant Professor, Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Vellore District, Tamil Nadu, India.
One test can save your life. Know what a Follicle Stimulating Hormone(FSH) is, why you should have it, who should get it, and where can you get tested as well as get your results fast. If you want to read more about Follicle Stimulating Hormone(FSH), click the link below.
Visit: https://www.labfinder.com/labexams/follicle-stimulating-hormone-fsh/ and get tested now!
Steroid hormones can be grouped into 2 classes, corticosteroids (typically made in the adrenal cortex, hence cortico-) and sex steroids (typically made in the gonads or placenta).
Steroid Hormones - Dr. P. Saranraj, Assistant Professor, Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Vellore District, Tamil Nadu, India.
One test can save your life. Know what a Follicle Stimulating Hormone(FSH) is, why you should have it, who should get it, and where can you get tested as well as get your results fast. If you want to read more about Follicle Stimulating Hormone(FSH), click the link below.
Visit: https://www.labfinder.com/labexams/follicle-stimulating-hormone-fsh/ and get tested now!
Hormonal control of the testicular function, with emphasis made on the role played by hormones or the endocrine system on the function of the testis and its importance in reproduction.
This power point presentation deals with the various types of glands present in human body. We have discussed here about Major Endocrine Glands in human body, their structure, their location, the hormones they produce and the effect of those hormones. We have also discussed about disorders that may occur with abnormal hormonal secretions, Lastly we have discussed about the mechanisms in which the hormone works inside human body
Immunoassay is a biochemical test that estimate or asses the presence or concentration of a macromolecule (antigen) in a solution (eg-blood) through the use of an antibody or immunoglobulin(Ig). The macromolecule called "analyte". Analytes in biological liquids such as blooed serum, biological fuid and urine are frequently measured using immunoassays ( for medical and research purposes).
Identification of the positively selected genes governing host-pathogen arm r...Atai Rabby
Bacterial evolution is due to the adaptive nature of the core bacterial genomes that plays critical role in diversification, fitness and adaptation of the species to different environment and host. Since Vibrio cholerae represents an appropriate model organism for studying the interplay of environment and host driven factors shaping the microbial genome structure and function, the current study aims to identify genes that are under these strong forces in V. cholerae. Here, we employed a comparative genomics approach to identify genes that are under positive selection in ten strains of Vibrio sp. including four pathogenic V. cholerae strains. From the available genome sequence data, a total of 422 orthologous genes were identified by reciprocal BLAST best-hit method, recombination breakpoint frequency analysis and tree comparison method. These 422 genes, representing the core genome of Vibrio sp., constituted the dataset to be analyzed for evolutionary selections. The analysis of natural selection, based on Maximum Likelihood method on synonymous and non-synonymous substitution rate, confirms the hypothesis that the bacterial core genomes are mostly under purifying selection with a few positively selected regions. However, our finding also reveals that positively selected sites in the Vibrio genome occur in a wide range of different genes encompassing diverse functional pathways including cell surface proteins (e.g. outer membrane-specific lipoprotein transporter/assembly proteins etc.), cell motility proteins (e.g. flagellar motor switch proteins, flagellar hook and assembly proteins), nutrient acquisition (e.g. amino acid, carbohydrate and phosphate ABC transporters), DNA repair and transcription related proteins. Interestingly, these positively selected gene products are directly involved with host-pathogen interactions and fitness in gastrointestinal environment. Therefore, the collective evidences of these positively selected genes spanning several pathways raise the possibility of their involvement in evolutionary arms races with other bacteria, phages, and/or the host immune system. This finding points to the natural selections which is the responsible factor for the diversification of Vibrio genus.
D4476, a cell-permeant inhibitor of CK1, potentiates the action of Bromodeoxy...Atai Rabby
To elucidate the mechanism of bromodeoxyuridine (BrdU) induced cellular senescence, we treated HeLa cells with D4476, a potent and specific inhibitor of casein kinase 1(CK1). We found that D4476 (10µM) treatment could arrest cell growth at G1 stage and induced cellular senescence when treated together with BrdU (10µM). However neither D4476 nor BrdU can induce cellular senescence alone, at a concentration of 10µM. These results suggest that the targets of CK1 may be involved in maintaining normal cellular process and their inactivation potentiates BrdU to induce senescence like phenomena.
Identifying Antibiotics posing potential Health Risk: Microbial Resistance Sc...Atai Rabby
The present study was undertaken to investigate the trends of antimicrobial resistance and identify antibiotics that are posing public health risk due to resistant microbes in Bangladesh. Antimicrobial resistance data of Bangladesh for last 10 years were searched out and compared with corresponding antibiotic consumption rates. In this study, a factor is introduced to identify the therapeutic sub-class of antibiotics that are mostly threatened by growing antimicrobial resistance. Highly resistance trend against several antibiotics such as cloxacillin, ampicillin, metronidazole, oxacillin, amoxicillin, tetracycline, cotrimoxazole, penicillin etc. were also indentified. Heat map analysis of this study revealed that nine antimicrobial agents: metronidazole, amoxicillin, tetracycline, cotrimoxazole, cephadine, penicillin, ciprofloxacin, doxycycline and nalidixic acid are associated with public health risk due to growing bacterial resistance. This study would significantly contribute in minimizing development and spread of antibiotic resistance by revealing the microbial resistance scenario and aid the effective antibiotic treatment options in Bangladesh.
Antiviral drugs are a class of medication used specifically for treating viral infections.Like antibiotics for bacteria, specific antivirals are used for specific viruses. Unlike most antibiotics, antiviral drugs do not destroy their target pathogen; instead they inhibit their development.
Actin filaments, usually in association with myosin, are responsible for many types of cell movements. Myosin is the prototype of a molecular motor—a protein that converts chemical energy in the form of ATP to mechanical energy, thus generating force and movement. The most striking variety of such movement is muscle contraction, which has provided the model for understanding actin-myosin interactions and the motor activity of myosin molecules. However, interactions of actin and myosin are responsible not only for muscle contraction but also for a variety of movements of nonmuscle cells, including cell division, so these interactions play a central role in cell biology. Moreover, the actin cytoskeleton is responsible for the crawling movements of cells across a surface, which appear to be driven directly by actin polymerization as well as actin-myosin interactions.
The thyroid hormones, triiodothyronine (T3) and its prohormone, thyroxine (T4), are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Iodine is necessary for the production of T3 and T4. A deficiency of iodine leads to decreased production of T3 and T4, enlarges the thyroid tissue and will cause the disease known as simple goitre. The major form of thyroid hormone in the blood is thyroxine (T4), which has a longer half-life than T3.The ratio of T4 to T3 released into the blood is roughly 20 to 1. T4 is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine (T1a) and thyronamine (T0a). All three isoforms of the deiodinases are selenium-containing enzymes, thus dietary selenium is essential for T3 production.
Parathyroid hormone (PTH), parathormone or parathyrin, is secreted by the chief cells of the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calcium (Ca2+) in the blood, whereas calcitonin (a hormone produced by the parafollicular cells (C cells) of the thyroid gland) acts to decrease calcium concentration. PTH acts to increase the concentration of calcium in the blood by acting upon the parathyroid hormone 1 receptor (high levels in bone and kidney) and the parathyroid hormone 2 receptor (high levels in the central nervous system, pancreas, testis, and placenta).PTH half-life is approximately 4 minutes. It has a molecular mass of 9.4 kDa.
The classical GI hormones are secreted by epithelial cells lining the lumen of the stomach and small intestine. These hormone-secreting cells - endocrinocytes - are interspersed among a much larger number of epithelial cells that secrete their products (acid, mucus, etc.) into the lumen or take up nutrients from the lumen. GI hormones are secreted into blood, and hence circulate systemically, where they affect function of other parts of the digestive tube, liver, pancreas, brain and a variety of other targets.
In mammals, the adrenal glands (also known as suprarenal glands) are endocrine glands that sit at the top of the kidneys. They are chiefly responsible for releasing hormones in response to stress through the synthesis of corticosteroids such as cortisol and catecholamines such as adrenaline (epinephrine) and noradrenaline. They also produce androgens in their innermost cortical layer. The adrenal glands affect kidney function through the secretion of aldosterone, and recent data (1998) suggest that adrenocortical cells under pathological as well as under physiological conditions show neuroendocrine properties; within normal adrenal glands, this neuroendocrine differentiation seems to be restricted to cells of the zona glomerulosa and might be important for an autocrine regulation of adrenocortical function.
DNA extraction is an important step in molecular assays and plays a vital role in obtaining highresolution results in gel-based systems, particularly in the case of cereals with high content of interfering components in the early steps of DNA extraction.This is a rapid miniprep DNA extraction method, optimized for rice, which was achieved via creating some modifications in present DNA extraction methods, especially in first step of breaking down and lyses of cell wall, and the use of cheap and frequent chemicals, found in every lab, in the next steps. The normal quality and quantity was obtained by the method. The PCR based assays also revealed the efficiency of the method.
The advantages of this method are: 1- it is applicable with both dry and fresh samples, 2- no need to large weight samples, 3- no need to liquid nitrogen and 4- easy, rapid and applicable in every laboratory.
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction (Bitner, Kuempel 1981).
Restriction mapping is a useful way to characterise a particular DNA molecule. It enables us to locate and isolate DNA fragments for further study and manipulation. The relative location of different restriction enzyme sites to each other are determined by enzymatic digest of the DNA with different restriction enzymes, alone and in various combinations.The digested DNA is separated by gel electrophoresis and the fragment sizes that have been generated are used to build the 'map' of sites of the fragment. The map lets us know 'where we are' in the linear DNA macromolecule.
Mesurement of cretinine kinase from blood of a cardiac patientAtai Rabby
The development of sensitive spectrophotometer able to measure a wide range of wavelengths has allowed natural substrates and measurements in the ultraviolet region of the spectrum to be used in clinical tests. Assays using natural substrates and measurements in the ultraviolet region are the basis of most of the commonly used methods to determine enzyme activities in clinical biochemistry. Typically, they continuously measure the absorbances of NAD+ or NADP+ at 340 nm. Figure shows the reactions used to monitor the activities of creatine kinase (CK) in clinical laboratories by measuring changes in the absorbance of NAD+ or NADP+. Clinical investigations using this enzyme can be used to detect muscle damage..
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
Gonadal hormone
1. What is Gonad?
The gonad is the organ that makes gametes.
The gonads in males are the testes and the
gonads in females are the ovaries.
The product, gametes, are haploid germ
cells. For example, spermatozoon and egg
cells are gametes.
The presence of the SRY (Sex-determining
region Y) gene, located on the Y chromosome
and encoding the testeis determining factor,
determines male sexual differentiation.
Reproduction – hormones of the
reproductive system
Brain-hypothalamic hormones
Gonadotropin-releasing hormone
(GnRH or LHRH).
Gonadotropin release-inhibitory
factors (GnRIF; such as dopamine)
Pituitary hormones: Gonadotropins
Follicle-stimulating Hormone (FSH):
controls gonadal growth
Luteinizing Hormone (LH): controls
gamete maturation and release
Gonadal hormones (steroids)
11-ketotestosterone (androgen):
important for spermatogenesis
17-estradiol (estrogen): important for
oogenesis
Progesterone or its derivatives
(progestins): induce gamete
maturation in males and females and
ovulation in females
Gonads & Hormones
2. GonadalHormone in Male Gonadal Hormone in Female
Ovulation and Corpus Luteum formation Luteinizing hormone (LH)
In males, where LH had also been called
interstitial cell-stimulating hormone (ICSH), it
stimulates Leydig cell production of
testosterone.
Hormone produced by the anterior pituitary
gland
In females, an acute rise of LH ("LH surge")
triggers ovulation and development of the
corpus luteum
It acts synergistically with FSH.
3. Structure of LH
LH is a heterodimeric glycoprotein.
Each monomeric unit is a glycoprotein molecule
One alpha and one beta subunit make the full, functional protein.
The alpha subunits of LH contain 92 amino acids in human but 96 amino acids in almost all other
vertebrate species. The carbohydrate moiety is linked to the asparagine at positions 52 and 78.
LH beta subunit have 120 amino acids (LHβ) that confers its specific biologic action and is
responsible for the specificity of the interaction with the LH receptor.
The biologic half-life of LH is 20 minutes, shorter than that of FSH (3–4 hours) and hCG (24
hours).
Genes
The gene for the alpha subunit is located on
chromosome 6q12.21.
The LH beta subunit gene is localized in the
LHB/CGB gene cluster on chromosome
19q13.32.
In contrast to the alpha gene activity, beta LH
subunit gene activity is restricted to the pituitary
gonadotropic cells. It is regulated by the
gonadotropin-releasing hormone from the
hypothalamus.
Activity
In both males and females, LH is essential for
reproduction.
In the male, LH acts upon the Leydig cells of the
testis and is responsible for the production of
testosterone, an androgen that exerts both
endocrine activity and intratesticular activity on
spermatogenesis.
4. LH is necessary to maintain luteal function for the first two weeks of the menstrual cycle. If pregnancy occurs, LH levels
will decrease, and luteal function will instead be maintained by the action of hCG (a hormone very similar to LH but
secreted from the new placenta).
In females, LH supports theca cells in the ovary that provide androgens and hormonal precursors for estradiol
production.
At the time of menstruation, FSH initiates follicular growth, specifically affecting granulosa cells.
With the rise in estrogens, LH receptors are also expressed on the maturing follicle, which causes it to produce
more estradiol.
When the follicle has fully matured, a spike in estrogen production by the follicle stimulates a positive feedback
loop in the hypothalamus that stimulates the release of LH from the anterior pituitary.
This increase in LH production only lasts for 24 to 48 hours.
This "LH surge" triggers ovulation, thereby not only releasing the egg from the follicle, but also initiating the
conversion of the residual follicle into a corpus luteum that, in turn, produces progesterone to prepare the
endometrium for a possible implantation.
Secretion
pattern of LH
5. Follicle-stimulating hormone (FSH)
FSH and luteinizing hormone (LH) act
synergistically in reproduction. Specifically, an
increase in FSH secretion by the anterior
pituitary causes ovulation.
It is synthesized and secreted by
gonadotrophs of the anterior pituitary gland.
FSH regulates the development, growth,
pubertal maturation, and reproductive
processes of the body.
FSH is a glycoprotein. Each monomeric unit is a
protein molecule with a sugar attached to it; two of
these make the full, functional protein.
Its structure is similar to those of LH, TSH, and
hCG.
The protein dimer contains 2 polypeptide units,
labeled alpha and beta subunits. The alpha
subunits of LH, FSH, TSH, and hCG are identical,
and contain 92 amino acids. The beta subunits
vary. FSH has a beta subunit of 111amino acids
(FSH β), which confers its specific biologic action
and is responsible for interaction with the FSH-
receptor.
The half-life of FSH is 3–4 hours.
Structure
Activity
FSH regulates the development, growth, pubertal maturation, and reproductive processes of the
human body.
FSH enhances the production of androgen-binding protein by the Sertoli cells of the testes
by binding to FSH receptors on their basolateral membranes, and is critical for the initiation of
spermatogenesis.
Effects in males
FSH stimulates primary spermatocytes to undergo the first division of meiosis, to form
secondary spermatocytes.
6. Effects in females
FSH stimulates the growth
and recruitment of immature
ovarian follicles in the ovary.
In early (small) antral
follicles, FSH is the major
survival factor that rescues the
small antral follicles (2–5 mm
in diameter for humans) from
apoptosis (programmed death
of the somatic cells of the
follicle and oocyte).
Gonadotropin-releasing hormone (GnRH),
Also known as Luteinizing-hormone-releasing hormone (LHRH) and luliberin, is a trophic peptide
hormone responsible for the release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH)
from the anterior pituitary.
GnRH is synthesized and released from neurons within the hypothalamus.
GnRH is considered a neurohormone, a hormone produced in a specific neural cell and released
at its neural terminal.
At the pituitary, GnRH stimulates the synthesis and secretion of the gonadotropins, follicle-
stimulating hormone (FSH), and luteinizing hormone (LH).
Low-frequency GnRH pulses lead to FSH release, whereas high-frequency GnRH pulses stimulate
LH release.
8. Testosterone
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles,
birds, and other vertebrates.
In mammals, testosterone is primarily secreted in the testicles of males and the ovaries of
females, although small amounts are also secreted by the adrenal glands.
It is the principal male sex hormone and an anabolic steroid.
In men, testosterone plays a key role in the development of male reproductive tissues such as the
testis and prostate as well as promoting secondary sexual characteristics such as increased muscle,
bone mass, and the growth of body hair.
Testosterone
Biosynthesis of testosterone
The largest amounts of testosterone (>95%) are produced by the testes in men.
It is also synthesized in far smaller quantities in women by the thecal cells of the ovaries, and by the
placenta,
The zona reticularis of the adrenal cortex and skin in both sexes also produces in small quantities.
In the testes, testosterone is produced by the Leydig cells. The male generative glands also contain
Sertoli cells which require testosterone for spermatogenesis.
9. Testosterone is primarily synthesized in Leydig cells.
The number of Leydig cells in turn is regulated by luteinizing
hormone (LH) and follicle stimulating hormone (FSH).
The amount of testosterone produced by existing Leydig cells is
under the control of LH which regulates the expression of 17-β
hydroxysteroid dehydrogenase (HSD).
When testosterone levels are low, gonadotropin-releasing
hormone (GnRH) is released by the hypothalamus which in turn
stimulates the pituitary gland to release FSH and LH. These later
two hormones stimulate the testis to synthesize testosterone.
Finally increasing levels of testosterone through a negative
feedback loop act on the hypothalamus and pituitary to inhibit the
release of GnRH and FSH/LH respectively.
Regulation of Testosterone Biosynthesis
Biosynthesis of Estrogen:
Estrogens, in females, are produced primarily by the ovaries, and during pregnancy, the placenta.
Follicle-stimulating hormone (FSH) stimulates the ovarian production of estrogens by the
granulosa cells of the ovarian follicles and corpora lutea.
Some estrogens are also produced in smaller amounts by other tissues such as the liver,
adrenal glands, and the breasts.
In females, synthesis of estrogens starts in theca interna cells in the ovary, by the synthesis
of androstenedione from cholesterol.
10. Estrogen
Estriol
Estradiol
Estrone
They are the primary female sex hormones.
Like all steroid hormones, estrogens readily diffuse across the cell membrane. Once inside
the cell, they bind to and activate estrogen receptors which in turn modulate the expression
of many genes. Additionally, estrogens have been shown to activate a G protein-coupled
receptor, GPR30.
Estradiol is the predominant estrogen during reproductive years
During menopause, estrone is the predominant circulating estrogen
during pregnancy estriol is the predominant circulating estrogen
Function of Estrogen
Promote formation of female secondary sex characteristics
Accelerate metabolism
Increase fat stores
Stimulate endometrial growth
Increase uterine growth
Increase vaginal lubrication
Thicken the vaginal wall
Maintenance of vessel and skin
Reduce bone resorption, increase bone formation
Reduce muscle mass
11. Progesterone
Progesterone also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in
the female menstrual cycle, pregnancy and embryogenesis of humans and other species.
Progesterone belongs to a class of hormones called progestogens, and is the major naturally
occurring human progestogen.
Progesterone is produced in the ovaries (by the corpus
luteum), the adrenal glands and, during pregnancy, in the
placenta.
Progesterone is also stored in adipose (fat) tissue.
Progesterone
Level of Progesterone
In women, progesterone
levels are relatively low during
the preovulatory phase of the
menstrual cycle, rise after
ovulation, and are elevated
during the luteal phase, as
shown in diagram below.
Progesterone levels tend to
be < 2 ng/ml prior to ovulation,
and > 5 ng/ml after ovulation.
If pregnancy occurs, human
chorionic gonadotropin is
released maintaining the
corpus leuteum allowing it to
maintain levels of
progesterone.
12. HYPOTHALAMUS RELEASES GONADOTROPIN-RELEASING HORMONE (GnRH). This stimulates the
anterior pituitary to release FSH and LH.
FSH STIMULATES MATURATION OF PRIMARY OOCYTE IN AN IMMATURE FOLLICLE.
FOLLICLE PRODUCES ESTROGEN. Estrogen: (A) builds the uterine wall (the endometrium); (B) inhibits
secretion of FSH.
HIGH LEVELS OF ESTROGEN FURTHER STIMULATE SECRETION OF LH BY ANTERIOR PITUITARY.
This plus FSH also causes ovulation of the secondary oocyte – leaving follicle without egg (the corpus
luteum).
CORPUS LUTEUM SECRETES ESTROGEN AND PROGESTERONE. This maintains the endometrium
for 15-16 days and inhibits LH.
(If oocyte is not fertilized and implanted in the uterine wall) CORPUS DEGENERATES (TO CORPUS
ALBICANS) AND STOPS PRODUCING ESTROGEN AND PROGESTERONE.
WITHOUT ESTROGEN AND PROGESTERONE, ENDOMETRIUM BREAKS DOWN – MENSTRUATION
OCCURS. Menstruation is the sloughing off of the enlarged endometrial wall along with blood and mucous.
DECREASE IN PROGESTERONE AND LH. Low LH causes secretion of FSH by pituitary again. The
cycle repeats.
HORMONAL REGULATION IN NONPREGNANT FEMALE
(UTERINE CYCLE)