Genetically modified insects show promise for improving the sterile insect technique used in agriculture and public health. The sterile insect technique works by releasing sterile male insects that outcompete wild males for mates. This reduces the population over time. Genetic modifications can enhance this approach by adding visible markers or making the insects more competitive. Transgenic insects directly impact disease transmission by disrupting parasites inside the insect. Paratransgenic methods modify insect symbionts to express proteins harmful to pathogens. Several programs have successfully used sterile and transgenic insects to control pests like pink bollworm and disease vectors such as mosquitoes. Genetic improvements continue to enhance sterile insect techniques.
The document discusses the Sterile Insect Technique (SIT) for controlling insect pest populations. It provides background on the history and development of SIT, including its initiation in the 1930s to control screwworm fly. SIT involves mass rearing insects, sterilizing males via radiation or chemicals, and releasing the sterile males to mate with wild females. This results in no offspring and population decline over time. Current SIT targets include various fly and mosquito species. Requirements for effective SIT implementation include methods for mass rearing, sterilization without affecting male competitiveness, and overwhelming the native population ratio with sterile insects. The technique has successfully eradicated several pests and provides a species-specific
Biotechnological approaches can be used in entomological research for developing transgenic insect-resistant crops, genetically modifying insects and biocontrol agents, and performing DNA fingerprinting of insects. Key approaches include using recombinant DNA technology to develop transgenic crops expressing genes from Bacillus thuringiensis that produce insecticidal proteins, genetically engineering plants to produce other insecticidal compounds, and using techniques like RNA interference to alter insect behaviors. These methods help increase crop yields by providing resistance against insect pests while reducing environmental impacts from pesticide use.
The successful management of a pest by means of another living organism (parasitoids, predators and pathogens) that are encouraged and disseminated by man is called biological
control. In such programme the natural enemies are introduced, encouraged, multiplied by artificial means and disseminated by the man with his own efforts instead of leaving it to nature.
Chemical pest control uses pesticides, which are chemicals that prevent, destroy, or repel pests. Pesticides are classified based on their target organisms like insects, weeds, and fungi. They also vary in their mode of action, such as contact, systemic, fumigant, and stomach poisons. Chemical pest control can effectively control pests but overuse risks developing pest resistance, eliminating natural enemies, and polluting the environment through residues in food and water contamination. Proper use of pesticides can provide agricultural benefits while minimizing disadvantages to health and ecology.
Dr. Pusztai fed genetically modified potatoes and non-modified control potatoes to rats in experiments. He found immune system and intestinal damage in rats that ate the GM potatoes but not in rats eating the non-GM potatoes. However, the Royal Society concluded the experiments were flawed with inappropriate controls and few rats, and found no evidence GM potatoes were harmful. The controversy highlighted disagreements over GM food safety and regulation between Pusztai and groups supporting biotechnology.
Economical basis of IPM - Economic Thresholdskhalil amro
The document discusses key concepts in integrated pest management (IPM) theory including the economic injury level (EIL), economic threshold (ET), and tolerance levels. The EIL is the pest density that causes economic damage equal to the cost of control. The ET is slightly below the EIL to allow time for control actions before losses reach the EIL. Periodic scouting is needed to determine pest densities and understand pest-damage relationships in order to establish appropriate control thresholds. Factors like crop value, control costs, and damage coefficients are considered in EIL calculations. Limitations to EIL and ET concepts include difficulties estimating variables and incorporating external factors.
The document provides information about the Sterile Insect Technique (SIT) for pest control. It defines SIT as a method using area-wide releases of sterile insects to reduce fertility of the target pest population. The procedure for SIT involves mass rearing the pest insect, sterilizing them commonly using irradiation, and wide-scale release of the sterile insects to mate with wild insects and reduce progeny. Examples of successful SIT programs that achieved eradication include the screwworm fly in North America in the 1950s-1980s and the tsetse fly in Zanzibar in the 1990s. SIT continues to be used against pests like the Mediterranean fruit fly and research is ongoing in
This document summarizes techniques for evaluating the effectiveness of releasing natural enemies for biological control of pests. It describes four main techniques:
1) Correlation of pest and natural enemy populations to show control.
2) Experimental addition, exclusion, and interference methods to directly measure control. Exclusion involves removing natural enemies from plots while addition entails introducing them.
3) Specific interference techniques include using insecticides, hand removal, manipulating other species, and traps.
4) Population modeling can also demonstrate control by mathematically modeling pest and natural enemy interactions.
The document discusses the Sterile Insect Technique (SIT) for controlling insect pest populations. It provides background on the history and development of SIT, including its initiation in the 1930s to control screwworm fly. SIT involves mass rearing insects, sterilizing males via radiation or chemicals, and releasing the sterile males to mate with wild females. This results in no offspring and population decline over time. Current SIT targets include various fly and mosquito species. Requirements for effective SIT implementation include methods for mass rearing, sterilization without affecting male competitiveness, and overwhelming the native population ratio with sterile insects. The technique has successfully eradicated several pests and provides a species-specific
Biotechnological approaches can be used in entomological research for developing transgenic insect-resistant crops, genetically modifying insects and biocontrol agents, and performing DNA fingerprinting of insects. Key approaches include using recombinant DNA technology to develop transgenic crops expressing genes from Bacillus thuringiensis that produce insecticidal proteins, genetically engineering plants to produce other insecticidal compounds, and using techniques like RNA interference to alter insect behaviors. These methods help increase crop yields by providing resistance against insect pests while reducing environmental impacts from pesticide use.
The successful management of a pest by means of another living organism (parasitoids, predators and pathogens) that are encouraged and disseminated by man is called biological
control. In such programme the natural enemies are introduced, encouraged, multiplied by artificial means and disseminated by the man with his own efforts instead of leaving it to nature.
Chemical pest control uses pesticides, which are chemicals that prevent, destroy, or repel pests. Pesticides are classified based on their target organisms like insects, weeds, and fungi. They also vary in their mode of action, such as contact, systemic, fumigant, and stomach poisons. Chemical pest control can effectively control pests but overuse risks developing pest resistance, eliminating natural enemies, and polluting the environment through residues in food and water contamination. Proper use of pesticides can provide agricultural benefits while minimizing disadvantages to health and ecology.
Dr. Pusztai fed genetically modified potatoes and non-modified control potatoes to rats in experiments. He found immune system and intestinal damage in rats that ate the GM potatoes but not in rats eating the non-GM potatoes. However, the Royal Society concluded the experiments were flawed with inappropriate controls and few rats, and found no evidence GM potatoes were harmful. The controversy highlighted disagreements over GM food safety and regulation between Pusztai and groups supporting biotechnology.
Economical basis of IPM - Economic Thresholdskhalil amro
The document discusses key concepts in integrated pest management (IPM) theory including the economic injury level (EIL), economic threshold (ET), and tolerance levels. The EIL is the pest density that causes economic damage equal to the cost of control. The ET is slightly below the EIL to allow time for control actions before losses reach the EIL. Periodic scouting is needed to determine pest densities and understand pest-damage relationships in order to establish appropriate control thresholds. Factors like crop value, control costs, and damage coefficients are considered in EIL calculations. Limitations to EIL and ET concepts include difficulties estimating variables and incorporating external factors.
The document provides information about the Sterile Insect Technique (SIT) for pest control. It defines SIT as a method using area-wide releases of sterile insects to reduce fertility of the target pest population. The procedure for SIT involves mass rearing the pest insect, sterilizing them commonly using irradiation, and wide-scale release of the sterile insects to mate with wild insects and reduce progeny. Examples of successful SIT programs that achieved eradication include the screwworm fly in North America in the 1950s-1980s and the tsetse fly in Zanzibar in the 1990s. SIT continues to be used against pests like the Mediterranean fruit fly and research is ongoing in
This document summarizes techniques for evaluating the effectiveness of releasing natural enemies for biological control of pests. It describes four main techniques:
1) Correlation of pest and natural enemy populations to show control.
2) Experimental addition, exclusion, and interference methods to directly measure control. Exclusion involves removing natural enemies from plots while addition entails introducing them.
3) Specific interference techniques include using insecticides, hand removal, manipulating other species, and traps.
4) Population modeling can also demonstrate control by mathematically modeling pest and natural enemy interactions.
Biological control (from the ecological viewpoint) is, “the action of parasites, predators, or pathogens in maintaining another organism's population density at a lower average than would occur in their absence.”
Release of large numbers of insectary reared natural enemies with the goal of “augmenting” natural enemy populations or “inundating” pest populations with natural enemies.
Here I would like to inform you in host selection process by the parasitiods.I hope It would increase your understanding on the steps involved n the host selection process.............................
Strategies for resistance management.pptxHarman Singh
This document discusses strategies for pesticide resistance management. It begins by defining pesticide resistance as populations of pests becoming less responsive to pesticides over time due to evolution. The rate of resistance development depends on factors like the frequency of resistant genes and selection pressure from pesticides. Resistance can be cross-resistance between pesticides with the same mode of action or multiple resistance to multiple pesticides via different mechanisms. Strategies to manage resistance include rotating pesticides, using mixtures, integrating pest control methods, and developing new pesticides. Proper pesticide use and resistance monitoring are important to delay resistance problems.
RNA interference technologies to control pests and pathogens - Steve Whyard -...OECD Environment
10-12 April 2019: The OECD Conference on RNAi based pesticides provided an overview on the current status and future possibilities for the regulation of externally applied dsRNA-based products that are proposed for use as pesticides. The event facilitated exchanges between policy makers, academia, industry on their implications in health, environment, and regulation.
This document discusses biological control of insect pests. It defines biological control as using natural enemies to reduce damage from insect pest populations. The document then covers the history of biological control from early efforts in 200 AD through the modern period. It discusses three approaches to biological control - classical biological control involving introducing exotic natural enemies, augmentative biological control involving adding natural enemies, and conservation biological control involving protecting existing natural enemies.
This document discusses cultural control methods for reducing pest damage to crops. Cultural control involves manipulating farming practices, like plowing, weeding, pruning, and crop rotation, to make the environment less favorable for pests. The practices may directly impact pest growth and reproduction or minimize their ability to attack plants. The goal is to control pests in an environmentally sound way without using pesticides. Some examples provided include intercropping cowpeas with sorghum to reduce stem borers, and destroying weed hosts to control citrus fruit sucking moths. Advantages are low cost and minimal environmental impact, while disadvantages include requiring long-term planning and not providing complete pest control.
Biotechnological approaches in Host Plant Resistance (HPR)Vinod Pawar
1. The document discusses biotechnological approaches to host plant resistance, including genetic manipulation of secondary plant substances and incorporation of resistant genes in crop varieties.
2. It provides examples of how genetic manipulation can enhance the production of compounds like terpenoids by modifying gene promoters, transcription factors, and subcellular localization.
3. It also gives an example of using marker-assisted backcrossing to introgress three bacterial blight resistance genes into the elite rice variety Samba Mahsuri, resulting in lines with high resistance and yield without compromising quality.
The document summarizes key aspects of pest surveillance using remote sensing and GIS techniques. It discusses pest surveillance methods like roving surveys and fixed plot surveys to monitor pest populations. It also describes using remote sensing from different platforms like ground-based, airborne and spaceborne sensors to collect spectral data on crop health and pest stress. GIS is used to store spatial data collected through remote sensing and surveillance that can help with pest management and decision making.
The document discusses sterile insect techniques (SIT), where overwhelming numbers of sterile insects are released to mate with wild females. Females that mate with sterile males cannot produce offspring, reducing the population. New precision-guided SIT uses CRISPR/Cas9 to disrupt genes controlling fertility, producing only sterile males. A genetically engineered moth that prevents female offspring from surviving was released in New York to control crop-damaging moths, providing a more sustainable and cheaper alternative to insecticides.
The document discusses insects as a potential source of human food. It notes that over 2 billion people already consume insects as part of their traditional diets. Insects provide high quality protein and nutrients to support a growing global population. The document outlines the nutritional value of various edible insect species and products that can be derived from insects, such as dye, honey, silk and wax. It proposes that insects represent a sustainable and affordable alternative protein source that could help address future challenges in food production and security.
This document discusses the nutritional needs and requirements for rearing parasitoid insects artificially. It covers various topics such as evaluating nutritional needs through food analysis and carcass analysis. It describes the main nutritional requirements including nitrogen sources, lipids, carbohydrates, and other needs like vitamins and minerals. It also discusses other physiological requirements like digestion, respiration, hormones and teratocytes. Additional topics covered include physico-chemical factors, food presentation, sterilization, and conclusions regarding successes in rearing over 130 entomophagous species artificially.
the repeated use of the same chemical which has the same mode of action that leads to the loss of insect sensitivity and also heritable change would occur in the genome nothing but resistance that means the population not able to control with the normal dose need to develop resistant management strategies
This document discusses integrated pest management (IPM) strategies. IPM is a holistic approach that uses monitoring, identification, and action thresholds to determine when and how to address pest issues using cultural, physical, biological, or chemical methods. The goal is to prevent and control pests with minimal risk to humans, the environment, and other organisms. The document outlines IPM principles and provides examples of various control tactics within each category.
This document provides an overview of integrated pest management (IPM). It defines IPM as a pest management approach that uses multiple control strategies, including cultural, mechanical, biological and chemical tactics, to keep pest populations below economically damaging levels while minimizing risks to human health and the environment. The key principles of IPM include understanding pest biology and crop-pest interactions, advanced planning, balancing control costs and benefits, and monitoring pest populations to inform management decisions. The document discusses various IPM strategies and their advantages for improving farm profitability, reducing pest resistance and environmental impacts compared to reliance on pesticides alone.
Biotchnological approaches in insect pest control vikiVaibhav Wadhwa
This document discusses various biotechnological approaches used in agricultural control, including tissue culture techniques, rDNA technology, and development of transgenic crops. It focuses on rDNA technology, explaining how foreign genes can be directly or vector-mediated transferred into crop plants. The use of Bacillus thuringiensis (Bt) genes to develop insect-resistant crops is described in detail, including the mechanism of action of Bt toxins. The document also discusses using plant protease inhibitor genes to develop insect-resistant transgenic plants, with some successes and failures noted.
Silica nanoparticles show potential as a new insecticide for pest control. Researchers found the first record of using nanotechnology in agriculture by testing silica nanoparticles on insects. The nanoparticles proved effective at killing insects, demonstrating their potential as an environmentally-friendly alternative to traditional chemical pesticides.
This document provides an overview of genetically modified mosquitoes for vector control. It discusses the mosquito lifecycle and transmission of vector-borne diseases. Methods for vector control include the use of Wolbachia-infected mosquitoes, which have shown promise in suppressing dengue virus in laboratory and field trials by impairing pathogen development. The document also describes techniques using sterile insects like the sterile insect technique (SIT) and release of insects carrying a dominant lethal gene (RIDL). Field trials on the Cayman Islands demonstrated that Wolbachia-infected mosquitoes can successfully introduce and spread the infection within a native mosquito population. However, more studies are still needed before GM mosquitoes can be effectively used for vector control.
Radiation has many applications in science and medicine. It is used to induce mutations in plants to develop new varieties that are hardier and more resistant to pests. Food is irradiated to kill microbes and extend shelf life. The sterile insect technique releases sterile insects to control pest populations. In medicine, short-lived radioactive isotopes are used as tracers in diagnostic scans and tests. Radiation therapy also uses isotopes to treat cancer. Radioactive dating employs isotopes' decay rates to determine the ages of materials, helping date archaeological and geological samples.
Biological control (from the ecological viewpoint) is, “the action of parasites, predators, or pathogens in maintaining another organism's population density at a lower average than would occur in their absence.”
Release of large numbers of insectary reared natural enemies with the goal of “augmenting” natural enemy populations or “inundating” pest populations with natural enemies.
Here I would like to inform you in host selection process by the parasitiods.I hope It would increase your understanding on the steps involved n the host selection process.............................
Strategies for resistance management.pptxHarman Singh
This document discusses strategies for pesticide resistance management. It begins by defining pesticide resistance as populations of pests becoming less responsive to pesticides over time due to evolution. The rate of resistance development depends on factors like the frequency of resistant genes and selection pressure from pesticides. Resistance can be cross-resistance between pesticides with the same mode of action or multiple resistance to multiple pesticides via different mechanisms. Strategies to manage resistance include rotating pesticides, using mixtures, integrating pest control methods, and developing new pesticides. Proper pesticide use and resistance monitoring are important to delay resistance problems.
RNA interference technologies to control pests and pathogens - Steve Whyard -...OECD Environment
10-12 April 2019: The OECD Conference on RNAi based pesticides provided an overview on the current status and future possibilities for the regulation of externally applied dsRNA-based products that are proposed for use as pesticides. The event facilitated exchanges between policy makers, academia, industry on their implications in health, environment, and regulation.
This document discusses biological control of insect pests. It defines biological control as using natural enemies to reduce damage from insect pest populations. The document then covers the history of biological control from early efforts in 200 AD through the modern period. It discusses three approaches to biological control - classical biological control involving introducing exotic natural enemies, augmentative biological control involving adding natural enemies, and conservation biological control involving protecting existing natural enemies.
This document discusses cultural control methods for reducing pest damage to crops. Cultural control involves manipulating farming practices, like plowing, weeding, pruning, and crop rotation, to make the environment less favorable for pests. The practices may directly impact pest growth and reproduction or minimize their ability to attack plants. The goal is to control pests in an environmentally sound way without using pesticides. Some examples provided include intercropping cowpeas with sorghum to reduce stem borers, and destroying weed hosts to control citrus fruit sucking moths. Advantages are low cost and minimal environmental impact, while disadvantages include requiring long-term planning and not providing complete pest control.
Biotechnological approaches in Host Plant Resistance (HPR)Vinod Pawar
1. The document discusses biotechnological approaches to host plant resistance, including genetic manipulation of secondary plant substances and incorporation of resistant genes in crop varieties.
2. It provides examples of how genetic manipulation can enhance the production of compounds like terpenoids by modifying gene promoters, transcription factors, and subcellular localization.
3. It also gives an example of using marker-assisted backcrossing to introgress three bacterial blight resistance genes into the elite rice variety Samba Mahsuri, resulting in lines with high resistance and yield without compromising quality.
The document summarizes key aspects of pest surveillance using remote sensing and GIS techniques. It discusses pest surveillance methods like roving surveys and fixed plot surveys to monitor pest populations. It also describes using remote sensing from different platforms like ground-based, airborne and spaceborne sensors to collect spectral data on crop health and pest stress. GIS is used to store spatial data collected through remote sensing and surveillance that can help with pest management and decision making.
The document discusses sterile insect techniques (SIT), where overwhelming numbers of sterile insects are released to mate with wild females. Females that mate with sterile males cannot produce offspring, reducing the population. New precision-guided SIT uses CRISPR/Cas9 to disrupt genes controlling fertility, producing only sterile males. A genetically engineered moth that prevents female offspring from surviving was released in New York to control crop-damaging moths, providing a more sustainable and cheaper alternative to insecticides.
The document discusses insects as a potential source of human food. It notes that over 2 billion people already consume insects as part of their traditional diets. Insects provide high quality protein and nutrients to support a growing global population. The document outlines the nutritional value of various edible insect species and products that can be derived from insects, such as dye, honey, silk and wax. It proposes that insects represent a sustainable and affordable alternative protein source that could help address future challenges in food production and security.
This document discusses the nutritional needs and requirements for rearing parasitoid insects artificially. It covers various topics such as evaluating nutritional needs through food analysis and carcass analysis. It describes the main nutritional requirements including nitrogen sources, lipids, carbohydrates, and other needs like vitamins and minerals. It also discusses other physiological requirements like digestion, respiration, hormones and teratocytes. Additional topics covered include physico-chemical factors, food presentation, sterilization, and conclusions regarding successes in rearing over 130 entomophagous species artificially.
the repeated use of the same chemical which has the same mode of action that leads to the loss of insect sensitivity and also heritable change would occur in the genome nothing but resistance that means the population not able to control with the normal dose need to develop resistant management strategies
This document discusses integrated pest management (IPM) strategies. IPM is a holistic approach that uses monitoring, identification, and action thresholds to determine when and how to address pest issues using cultural, physical, biological, or chemical methods. The goal is to prevent and control pests with minimal risk to humans, the environment, and other organisms. The document outlines IPM principles and provides examples of various control tactics within each category.
This document provides an overview of integrated pest management (IPM). It defines IPM as a pest management approach that uses multiple control strategies, including cultural, mechanical, biological and chemical tactics, to keep pest populations below economically damaging levels while minimizing risks to human health and the environment. The key principles of IPM include understanding pest biology and crop-pest interactions, advanced planning, balancing control costs and benefits, and monitoring pest populations to inform management decisions. The document discusses various IPM strategies and their advantages for improving farm profitability, reducing pest resistance and environmental impacts compared to reliance on pesticides alone.
Biotchnological approaches in insect pest control vikiVaibhav Wadhwa
This document discusses various biotechnological approaches used in agricultural control, including tissue culture techniques, rDNA technology, and development of transgenic crops. It focuses on rDNA technology, explaining how foreign genes can be directly or vector-mediated transferred into crop plants. The use of Bacillus thuringiensis (Bt) genes to develop insect-resistant crops is described in detail, including the mechanism of action of Bt toxins. The document also discusses using plant protease inhibitor genes to develop insect-resistant transgenic plants, with some successes and failures noted.
Silica nanoparticles show potential as a new insecticide for pest control. Researchers found the first record of using nanotechnology in agriculture by testing silica nanoparticles on insects. The nanoparticles proved effective at killing insects, demonstrating their potential as an environmentally-friendly alternative to traditional chemical pesticides.
This document provides an overview of genetically modified mosquitoes for vector control. It discusses the mosquito lifecycle and transmission of vector-borne diseases. Methods for vector control include the use of Wolbachia-infected mosquitoes, which have shown promise in suppressing dengue virus in laboratory and field trials by impairing pathogen development. The document also describes techniques using sterile insects like the sterile insect technique (SIT) and release of insects carrying a dominant lethal gene (RIDL). Field trials on the Cayman Islands demonstrated that Wolbachia-infected mosquitoes can successfully introduce and spread the infection within a native mosquito population. However, more studies are still needed before GM mosquitoes can be effectively used for vector control.
Radiation has many applications in science and medicine. It is used to induce mutations in plants to develop new varieties that are hardier and more resistant to pests. Food is irradiated to kill microbes and extend shelf life. The sterile insect technique releases sterile insects to control pest populations. In medicine, short-lived radioactive isotopes are used as tracers in diagnostic scans and tests. Radiation therapy also uses isotopes to treat cancer. Radioactive dating employs isotopes' decay rates to determine the ages of materials, helping date archaeological and geological samples.
This document provides information about genetically modified organisms (GMOs). It defines a GMO as an organism whose genetic material has been altered using genetic engineering techniques. The document discusses how genetic engineering works by introducing DNA from other species. It then gives examples of common GMOs like Bt crops and Roundup Ready crops. The document also discusses debates around GMO safety and regulation. It concludes by discussing how genetically modified mosquitoes could help reduce diseases like malaria and how sterile insects were used to successfully eradicate screwworm flies in the southern US.
Biological pest control uses living organisms to reduce pest populations. There are three types of biological control strategies - importation, augmentation, and conservation. Importation involves introducing a pest's natural enemies into an area where they are not found naturally. Augmentation supplements existing natural enemy populations through additional releases. Conservation enhances conditions for natural enemies to survive and reproduce. Common natural enemies used in biological control include predators, parasites, and pathogens.
This document provides an overview of pest control measures. It begins by defining what a pest is and provides examples of common pests like insects, weeds, and diseases. It then discusses the history of major pest outbreaks like the bubonic plague and potato famine. The document outlines the evolution of pest control from primitive to modern techniques. It describes the four major categories of pests and various control methods like biological, mechanical, cultural, physical, genetic, chemical, and regulatory approaches. For each control method, examples are given to illustrate how it can be applied to manage different types of pests.
Genetic engineering alters the genes of organisms to produce beneficial effects for humans. It has improved crop yields and introduced traits like disease resistance. Scientists have genetically engineered microorganisms to help clean pollution from soil and water. While genetic engineering provides benefits, there are also risks like unintentionally creating allergenic foods or plants becoming invasive "superweeds". However, regulations aim to minimize these risks and the technology could help nutrition and public health when used carefully.
This document discusses the use of irradiation to preserve foods. It notes that high levels of food loss occur due to spoilage and diseases. Irradiation is presented as a physical preservation method that can inactivate microorganisms, parasites, insects and mites through ionizing radiation like gamma rays, x-rays and electron beams. The document provides a history of food irradiation research and regulation, and reviews how irradiation can be used to disinfest, extend shelf life, decontaminate and improve the quality of various foods like potatoes, meat and produce.
This document discusses biological control of plant diseases. Biological control involves using living organisms to control pests. It has received more attention recently. Some advantages are that it is specific to pests and cheaper after initial costs. Disadvantages include narrow effectiveness and high start-up expenses. Biological control agents include parasitoids, pathogens, and predators. Parasitoids lay eggs on or in a host insect and kill it. Pathogens infect insects and kill them or affect future generations. Predators are larger than prey and eat several. The document also discusses antagonists that compete with or produce toxins against plant pathogens. Common release methods are inoculative, where small numbers are released to spread, and augmentation, where organisms are mass
The document provides an overview of genetic engineering and its history. It discusses the basics of genetic engineering, which involves isolating and copying genetic material of interest using molecular cloning methods and inserting new DNA into the host genome. The history of genetic engineering is then explored, from early discoveries like Mendel's work with inheritance in peas to more modern developments like recombinant DNA techniques, PCR, and the creation of the first transgenic animal. A number of influential scientists in the field are also highlighted. The document aims to inform the reader about genetic engineering, related techniques, and its progression over time.
A gene is the fundamental physical and functional unit of heredity that is responsible for an organism's physical and inheritable characteristics. Genetic engineering involves manipulating or altering the structure of genes to create desired traits in an organism. If genetic material from another species is added, the resulting organism is called transgenic. Genetic engineering can also remove genetic material, creating a knock out organism.
Genetic engineering involves transferring DNA between organisms. It uses recombinant DNA techniques where the gene of interest is isolated and inserted into a vector like a plasmid or virus, which is then used to introduce the gene into a host cell. This allows the production of useful proteins like insulin through genetically modified bacteria. While genetic engineering has benefits like producing important medicines, there are also potential health and environmental risks to consider.
Prospectives Of Plant Products In Sustainable Developmentsjcc
1. The document discusses the potential of plant products in sustainable agriculture and pharmaceutical industries in India. It notes plant products can control insect populations naturally without environmental pollution.
2. Several plant essential oils and extracts are effective at inhibiting mycotoxins like aflatoxin B1 produced by fungi in stored crops. Many botanical pesticides have much higher LD50 values and are safer for humans than synthetic pesticides.
This document discusses genetically modified insects. It provides background on how genetically modified insects are created by inserting DNA from other organisms into insect genomes. The main purposes are to manage agricultural pests and spread of human diseases. The document outlines the history of using genetic modification techniques like sterile insect technique and transgenic methods. It discusses examples of genetically modified mosquitoes used against malaria and pink bollworm moths used against cotton pests. Both advantages like public health benefits and limitations like environmental risks are addressed.
Transgenic animals are produced by inserting foreign DNA into the animal's genome. There are several methods for producing transgenic animals. The first successful method involved microinjecting a rat growth hormone gene controlled by a promoter into mouse embryos, producing mice that grew larger. Other methods include using embryonic stem cells, viral vectors, cloning, and sperm-mediated gene transfer. Transgenic animals are useful for researching gene function and regulation, modeling human diseases, and potentially increasing agricultural production.
KnockOut mouse technology By Bikash karkiBikash Karki
The document summarizes the process of creating a knockout mouse through genetic engineering techniques. Key points:
- Knockout mice are created by "knocking out" or inactivating specific genes in embryonic stem cells taken from early mouse embryos.
- There are two main methods - homologous recombination, which precisely replaces a gene with an inactive version, and gene trapping, which randomly inserts DNA to disrupt gene function.
- Genetically modified stem cells are injected into mouse blastocysts to generate chimeric mice, and breeding is used to produce mice that are homozygous for the knocked out gene. Studying these mice helps reveal the function of the targeted gene.
The document discusses several methods to generate transgenic animals, including:
1. Microinjecting DNA directly into embryos, which was the first successful method for mice but has low efficiency in other species.
2. Using transposons to insert DNA randomly throughout the genome, which is effective for insects, fish, and mammals.
3. Employing lentiviral vectors to integrate foreign genes into the host genome, which has proven highly efficient in several species.
4. Incubating sperm with DNA and using intracytoplasmic sperm injection for fertilization, which has generated transgenic mice and rabbits.
Rishabh Maheshwari presents information on transgenic techniques. Transgenics involve introducing foreign DNA into a host organism's genome, typically using a mouse as the host. This allows for engineering organisms with DNA from another source as part of their genetic material. Common methods to create transgenic animals include DNA microinjection, retrovirus-mediated gene transfer, sperm-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic technology has applications in disease models, pharmaceutical production, agriculture, and industry. While it has benefits, there are also concerns regarding animal welfare and environmental impacts.
Transgenesis is the future of healthcare where the world is focusing on it so why not us? Let's delve into the exclusive depth of this transgenesis in the slide.
The sterile insect technique involves mass rearing and sterilizing insects, typically through irradiation, and releasing them in wild populations to reduce reproduction. When sterile males mate with wild females, no offspring are produced, lowering the next generation's population. This method was first developed to control screwworm flies damaging cattle in the US. It has since successfully eradicated several agricultural pests, including screwworm flies from North and Central America and Mediterranean fruit flies from parts of South America. Sterilization is achieved through either chemicals interfering with reproduction or radiation inducing sterility or genetic mutations.
Transgenic animals are animals whose genomes have been altered by the addition of foreign DNA. There are three main methods for creating transgenic animals: retroviral vector method, DNA microinjection, and using engineered embryonic stem cells. Many transgenic animals have been created successfully for various purposes, including glowing zebrafish, faster growing salmon, Alzheimer's disease mouse models, and the first transgenic monkey. Transgenic technology holds promise for applications in agriculture, medicine, and industry, but also raises ethical concerns and biosafety issues.
Fungi are the commonest pathogens in insects, with approximately 1000 species known to cause disease in arthropods.
most entomopathogenic fungi infect insects by direct penetration through the cuticle and killed by production of toxins.
Several entomopathogenic fungi, such as Metarhizium spp. And Beauveria spp., have been developed as environmentally friendly alternatives to chemical insecticides in biocontrol programs for agricultural pests and vectors of disease
Transgenic organisms and methods of their production.Garima
This document provides an overview of transgenic organisms. It begins with definitions of key terms like transgene, genome, plasmid, and restriction enzyme. It then discusses the history of transgenic research, including the first genetically modified organism created in 1973 and the first transgenic animals. The main methods used to produce transgenic animals are described as DNA microinjection, embryonic stem cell-mediated gene transfer, and retrovirus-mediated gene transfer. Current applications of transgenic organisms are outlined, such as glowing fish and insects used for pest control. The document concludes by discussing the importance of transgenic organisms in medicine, agriculture, and industry.
lec-7- parasitology. abdul salam najim mohammedsalamkrbooly
1. Enterobius vermicularis, also known as the pinworm, is a parasitic nematode classified in the phylum Nematoda. It infects the human large intestine and is the most common helminth infection in the United States and worldwide.
2. The pinworm life cycle involves ingestion of embryonated eggs, hatching of larvae in the small intestine, migration of worms to the colon where they mature over 7-8 weeks, and nighttime migration of gravid females to the perianal area to deposit eggs which can become infectious within 3-4 hours.
3. Pinworm infection is diagnosed by finding adult worms or eggs in the perianal area,
Genetic modification through recombination breeding j.dJagdeep Singh
This document discusses genetic manipulation through recombinant breeding and various genetic engineering techniques. It defines genetic manipulation as the manipulation of genetic material to produce specific results in an organism. It then discusses recombinant DNA and various modern genetic modification techniques used, including Agrobacterium tumefaciens mediated transformation, biolistic methods, microinjection, electroporation, and lipofection. Examples of genetically engineered crops and their traits are provided. Both advantages and risks of genetic engineering are mentioned.
Transgenic animals are created through genetic engineering by introducing foreign genes into the animal's genome. This allows the animal to produce proteins it would not normally make. Methods for creating transgenic animals include microinjection of DNA into fertilized eggs or embryonic stem cells. Transgenic animals have various applications including serving as disease models, producing pharmaceuticals in their milk (transpharmers), providing organs or tissues for transplantation (xenotransplantation), and enhancing food production. However, transgenic animal research also raises ethical issues regarding animal welfare and the environmental impacts of genetic modification.
Environmental issues associated with transgenic cropsSheetal Mehla
This document summarizes environmental issues associated with transgenic crops. It discusses direct effects on biodiversity and non-target organisms from GM crops. One example discussed is early research suggesting Bt corn may harm monarch butterflies, but later studies found large-scale cultivation does not significantly impact monarch populations. The document also covers pollen-mediated gene transfer between crops and wild varieties, as well as concerns about horizontal gene transfer and invasiveness of GM crops. Indirect effects from changes in farming practices associated with GM crops are also noted.
This document discusses reproductive and genetic control methods for insects, including sterilization techniques. It focuses on the Sterile Insect Technique (SIT), how it works to reduce insect populations by releasing sterile insects, and its successful use eradicating the screwworm fly. SIT involves rearing insects in a facility, sterilizing them with radiation or chemicals, and releasing large numbers to mate with wild insects, reducing future populations. Key applications include eliminating screwworms from islands and countries in North and Central America from the 1950s-1980s. The document also reviews advantages and limitations of SIT.
Introduction
History
Landmarks Events in Transgenic Livestock Research
Techniques/ Method for Gene Transfer
Examples of transgenesis
Importance
Application
Limitation
Issue related to Transgenic Technology
Ethical concerns and how to Overcome
Similar to Genetic Improvements to the Sterile Insect Technique for Agricultural & Public health Pests (20)
Bio-pesticides are naturally occurring substances from living organisms (natural enemies) or their products (microbial products, phytochemicals) or their by products (semio-chemicals that can control pests by nontoxic mechanisms.
Use of DNA Barcoding in InsectTaxonomyShweta Patel
DNA barcoding is a technique that uses a short, standardized DNA sequence from a gene to identify species. The most common gene used for animals is cytochrome c oxidase I from the mitochondria. DNA barcoding has proven useful for identifying species across various life forms, including insects, fish, butterflies, and true bugs. It provides benefits such as enabling non-specialists to identify specimens, identifying agricultural pests and disease vectors, and delimiting cryptic species. Major projects involving DNA barcoding aim to create a global reference library to classify thousands of species.
Screening Techniques for Different Insect Pests in Crop Plants Shweta Patel
This document discusses various screening techniques for different insect pests in crop plants. It describes procedures for developing and standardizing screening methods, including selecting seeds and screening sites. Several techniques are outlined for screening based on plant damage observed in the field, greenhouse, or laboratory. These include field, cage, and greenhouse screening. It also discusses techniques for screening based on insect responses like orientation, feeding, development, and fecundity. Examples of screening scales used for specific pests in different crops like rice, sorghum, cotton, sugarcane, rapeseed, and pulses are provided. Development and standardization of screening techniques is important for effective resistance breeding programs against insect pests.
Impact Of Electromagnetic radiations of biology and behaviour of Apis mellife...Shweta Patel
The document discusses a study on the impact of electromagnetic radiation from cell phone towers on honeybee colonies. The study involved placing beehives at different distances (0m, 200m, 400m, 600m, 800m) from a cell tower and measuring various honeybee activities and development over time. Results showed that colonies closer to the tower (<400m) had lower honey production, egg laying, brood development, and larval areas compared to farther colonies. This suggests electromagnetic radiation from cell towers may negatively affect honeybee health and colony development.
Rabindranath Tagore was a Bengali poet, philosopher, artist, and composer who was the first Asian to win the Nobel Prize in Literature in 1913. He wrote many poems, stories, novels, and plays and composed music and songs. Tagore played a crucial role in India's cultural renaissance in the 19th-early 20th century. He is known for writing the national anthems of India, Sri Lanka, and Bangladesh and for founding Shantiniketan university in West Bengal.
The document discusses plant disease forecasting. It provides information on:
1. The principles of disease forecasting are based on the nature of the pathogen, environmental effects on pathogen development, host response to infection, and grower activities.
2. Models for disease prediction include empirical, simulation, and general circulation models, but these models have limitations due to uncertainty and non-linear relationships.
3. Disease forecasts are used for strategic decision making like crop selection and tactical decisions around disease management measures. Successful forecasting requires reliability, simplicity, importance of the disease, and usefulness.
forecasting is the first step for IPM. forecasting reduce the protection cost.various models and software are now known to present days ,Which are useful in control the pest.
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
-------------------------------------------------------------------------------
Find out more about ISO training and certification services
Training: ISO/IEC 27001 Information Security Management System - EN | PECB
ISO/IEC 42001 Artificial Intelligence Management System - EN | PECB
General Data Protection Regulation (GDPR) - Training Courses - EN | PECB
Webinars: https://pecb.com/webinars
Article: https://pecb.com/article
-------------------------------------------------------------------------------
For more information about PECB:
Website: https://pecb.com/
LinkedIn: https://www.linkedin.com/company/pecb/
Facebook: https://www.facebook.com/PECBInternational/
Slideshare: http://www.slideshare.net/PECBCERTIFICATION
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
How to Fix the Import Error in the Odoo 17Celine George
An import error occurs when a program fails to import a module or library, disrupting its execution. In languages like Python, this issue arises when the specified module cannot be found or accessed, hindering the program's functionality. Resolving import errors is crucial for maintaining smooth software operation and uninterrupted development processes.
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
हिंदी वर्णमाला पीपीटी, hindi alphabet PPT presentation, hindi varnamala PPT, Hindi Varnamala pdf, हिंदी स्वर, हिंदी व्यंजन, sikhiye hindi varnmala, dr. mulla adam ali, hindi language and literature, hindi alphabet with drawing, hindi alphabet pdf, hindi varnamala for childrens, hindi language, hindi varnamala practice for kids, https://www.drmullaadamali.com
Genetic Improvements to the Sterile Insect Technique for Agricultural & Public health Pests
1.
2. GeneticGenetic
Improvements toImprovements to
the Sterile Insectthe Sterile Insect
Technique forTechnique for
Agricultural &Agricultural &
Public health PestsPublic health Pests Shweta
Patel
Id No.
42537
3. Sterile insect technique
The sterile insect technique (SIT) is an
environmentally friendly method for the
biological control of pests using area-wide
inundative release of sterile insects to
reduce reproduction in a field population
of the same species (IPPC, 2007)
4. History of
Sterilization
Irradiation of male insects
(USDA, 1950s)
Background
X-rays caused sterility in male insects (1916)
Dr. Edward Knipling (1954) in screw-worm fly
(Cochliomyia hominivorax) - subtropical America
livestock in Florida
Melon fly (Bactrocera cucurbitae) from Okinawa in
Japan(1972-1993) Koyama et al. 2004
Tse-tse fly( Glossina austeni)from Unguja Island
in Zanzibar,Tanzania(Vreysen et al.1996)
6. Requirements for SIT
• Insects can be reared and sterilized in large
quantities.
• Methods exist for distributing the sterile
insects throughout the target area so they
thoroughly mix with the wild population.
• The release is timed to coincide with the
reproductive period of the target insect.
• The released, sterile insects compete
successfully for mates in the natural
environment.
7. Continue…….
• The release ratio (sterile insects to
native, fertile insects) is large enough to
overcome the natural rate of increase of
the population, so that the trend in
population size is downward after the
first release.
• The target population is closed; i.e.,
there is no immigration of fertile insects
from outside the release zone.
8. Model for sterile insect
technique (SIT)
Generati
on
No.
virgin
females
in
area
No. sterile
males
released
per
generation
Ratio
sterile
to
fertile
males
%
females
mated
to
sterile
males
Pop. of
fertile
females
F1 10,00000 20,00000 2:1 66.7 3,33,333
F2 3,33,333 20,00000 6:1 85.7 47,619
F3 47,619 20,00000 42:1 97.7 1,107
F4 1,107 20,00000 1807:1 99.9 Less than 1
9. How SIT works
• When more sterile males are available
than fertile males, the likelihood of
mating with a sterile insect is high,
suppressing the reproductive output of
the fertile population.
• In generation 1, 2or3 of the males are
sterile, so 2or3 of the matings should
result in reproductive failure.
10. Continue…
• As the population of fertile males decreases,
the ratio of sterile to fertile increases,
depressing the population even faster.
• Once attaining a low level of fertile insects, it
is easy to maintain the population at low levels
with continued releases. In some cases, the
pests are eliminated(eradicated), so no further
releases are made
13. Definition-
A genetically modified organism (GMO) is an
organism whose genetic [material] has been
altered using techniques in genetics
generally known as recombinant DNA
technology.
Genetically modified insects are :-
•Insects With newly expressed
characteristics
•New characters – as a result of manipulation
of DNA in laboratory
•Changes - passed on to next generation
14. • Achieved by using gamma irradiation, UV
rays and mutagens like Ethyl methyl
sulphonate
• Till now 18 different genera have been
manipulated .
• First genetically transformed insect -
reported when wild type eye colour gene
was seen in a mutant strain of Drosophila.
• Next transformation was attempted in
mediterranean fruit fly in 1995 (Loukeris).
15. History of genetically
modified insect
•Produced as a result of gene manipulation, a
technique for genetic control of insects.
•In 1937,E.F.knipling-concept of genetic
control of insect pest.
•Stated with sterilization of Screw worm
flies, a serious pest of livestock.
16. Why Genetically modified
insects
• Benefit public health
• Enhance agricultural production
• Provide new forms of economically
useful insects.
18. TRANSGENIC INSECTS
• Insects with transgene
integrated into chromosome
• Transposable elements act as
vectors thereby carrying
transgenes into chromosome
(Finnegan,1989)
19. • Fusion of chromosome and transgene is
promoted by transposable elements that
cut and repair chromosomes
• Transgenes used for recognition of
transgenic insects are called markers
• Promoters are used to drive the expression
of markers (Coates,1999)
20. INTRODUCED TRANSGENES IN INSECT
INSECTS GENES CHARACTER
MODIFIED
1. Anopheles SM 1 Disease causing
ability destroyed
2. Culex Defensin Disease spreading
ability is lost
3. Silkworm Spider
flagelliform silk
Enhances quality of
silk protein
4. Wolbachia Attacin and
Cecopin
Infective capacity is
lost
5. Xylella S 1 Disease causing
capacity is absent
23. Transposable
elements
• Transposable elements-Mobile pieces of
DNA that do not remain fixed at one
genomic location but move from one site on
a chromosome to another(Liao,2000)
• Increase their copy number as they move
around among chromosomes within
individual organism.
24. Use of viral vectors
• Viral systems offer promising techniques
for expression of foreign genes
(Hahn,1992)
• Viral transducing systems allow long term
and stable cytoplasmic expression of
foreign DNA
• Viruses engineered with antisense RNA
are found complimentary to yellow fever
viral sequences
26. Sperm mediated
transformation
Factors like low reproductive rates and
egg properties prevent DNA introduction
So, virgin queens are inseminated with a
mixture of linearized DNA and semen
(Robinson,2000)
27. PARATRANSGENIC INSECTS
Paratransgenesis was first conceived by
Frank Richards (1996)
Paratransgenesis is a technique that
attempts to eliminate a pathogen from vector
populations through transgenesis of a
symbiont of the vector. The goal of this
technique is to control vector-borne diseases.
30. Chagas disease:
Is caued by parasite Trypanosoma cruzi
spread by kissing bug (Rhodnius
prolixus ) which is associated with the
symbiont Rhodococcus rhodnii.. The
strategy was to engineer R. rhodnii to
express proteins such as Cecropin A
that are toxic to T. cruzi or that block
the transmission of T. cruzi.
31. Requirements for
Paratransgenesis
• The Symbiotic bacteria can be grown in vitro
easily
• They can be genetically modified, such as
through transformation with a plasmid containing
the desired gene
• The engineered symbiont is stable and safe
• The association between vector and symbiont
cannot be attenuated
• Field delivery is easily handled
33. 1.Genetically modified
malaria causing
mosquitoes• Mosquitoes spread malaria and kill 2.7
million people per year world wide
(Rasgon,2007)
• Mosquitoes are engineered to produce
protein that disrupt malarial parasite life
cycle within insect .
• Gene (SM 1) prevents malarial parasite
from penetrating into mosquito mid gut and
reaching salivary glands (Braig and Yan,
2002)
34. Green fluorescent protein (GFP)
inserted into transgenic mosquitoes
make their eyes glow green under
UV light
Transgenic mosquitoes - With high
survival rate and lay more eggs
Anopheles stephensi is one of the
genetically engineered common
mosquito species to resist malaria
(Catteruccia, 2003)
35. The GM mosquito could
be identified by their
green fluorescent eyes
36. 2.Genetically modified
Yellow fever causing
mosquitoes
• Mosquito like Aedes aegypti spread
yellow fever
• Ken Olson, a virologist created GM
mosquito to replace these breeds.
• Produce antibacterial protein, limiting its
ability to transmit disease (Adelman,
2002)
37. 3. Sleeping
Sickness
• This disease is also referred to
as African Sleeping
sickness(Askoy,2003)
• It affect more than fifty
thousand people per year
• It is caused by Tsetse fly and
kissing bug
• Controlled by paratransgenesis
38. 4.Genetically modified
Dengue Fever causing
mosquitoes• Dengue Fever is caused by viruses transmitted
by mosquitoes Aedes aegypti
• It infects 50-100 million people annually with
2.5 billion worldwide at risk
• 6,000 of such GM mosquitoes have already
been released in the Malaysian forests in
January of this year.
• Oxitec scientists has led to such GM
mosquitoes also released in the wild in the
forests of the Cayman Islands.
40. 1.Pink boll
worm
• Sterile insect technique programme (SIT)
Protects more than 900,000 acres of cotton
• Million of male pink boll worm moth were
sterilized by irradiation(Pelloquin,1999)
• Moths are engineered to contain gene from jelly
fish(GFP)
• A lethal gene (t Ta) is introduced from
bacteria(Briggs,2001)
• It alters the metabolism of the moth larvae
41. 2. Med fruit fly
• Males are sterilized
by irradiation prior
to release
(Lobo,1999)
• Sterile males mate
with feral females
hindering female
reproduction
Medfly eggs
expressing GFP
42. 3. Pierce’s disease
• It is the lethal infection of grape vines
xylem by bacteria Xyllela
Species(Bextine,2004)
• This bacteria is carried by the vector
Glass Winged Sharp Shooter
• There is no control measure for this
disease
• Controlled by paratransgenesis
43. • Anti Xyllela effector
proteins (S 1)were isolated
and modified to carry anti
bacterial toxins against
Xyllela(Miller,2007)
• Others insects like Codling
Moth, Cabbage looper, Onion
fly and parasitoids like
Trybliographa species are
controlled under SIRM
programme.
44. 4. Transgenic Red
flour beetle
• It is a worldwide pest of stored
products
• Genes responsible for regulating
pheromone secretion are mutated
(Dabron, 2002)
• Specific gene expression is knocked
out by RNA interference.
46. RELEASED COMMERCIALLY
• Predatory mites-In 1997 in US.
• Pink bollworm-in 2001 in Mexico.
• Anapheles mosquito-In 2002 in New Delhi
and UP.
• Screw worm fly-Exported from Libya to
Kenya and Central America.
47. Hybrid Sterility
• Males & Females of different strains can
produce non-viable offspring
• Incompatible strains can be generated through
several ways
• Direct genetic manipulation
• Microbially-mediated (Cytoplasmic
Incompatibility)
• This phenomenon has been clearly demonstrated
in crosses between Heliothis virescens males
and Heliothis subflexa females (Laster et al.
1996)
48. Wolbachia and Reproduction
• Vertical transmission
cytoplasmic
inheritance Causes
male killing and
sterility in males
• Induces
parthenogenesis
• Cytoplasmic
incompatability
(conflict between
cytoplasmic and
nuclear components) Insect egg containing Wolbachia
49. Cytoplasmic Incompatability and
vertical transmission
• If both male and female
insects are infected with
Wolbachia – the progeny
will be infected
• If the female is infected
and the male is not
infected, the progeny will
all be infected.
• If the female is not
infected and the male is
infected there will not be
any progeny
50. RIDL
• RIDL (release of insects carrying a
dominant lethal)insects contain a genetic
modification that causes their offspring
to die, but the RIDL insects can live and
reproduce normally when they are fed a
diet containing a supplement.
• RIDL males are released to mate with wild
female pest insects; their progeny inherit
the RIDL gene and do not survive to
adulthood.
51. Inherited sterility in
insects
The inherited sterility in insects is induced
by substerilizing doses of ionizing
radiation. When partially sterile males
mate with wild females, the radiation-
induced deleterious effects are inherited
by the F1 generation. As a result, egg
hatch is reduced and the resulting
offspring are both highly sterile and
predominately male.
52. Continue…
• The silk worm Bombyx mori was
the first insect in which inherited
sterility was reported.
• Then inherited sterility was
reported in the greater wax moth
Galleria mellonella , codling moth
Cydia pomonella .
53. LIMITATIONS
• Instability of the introduced genes
• Transgenes were reported to get rapidly
lost under field conditions.
• Experimental release of transgenic
predatory mites showed that very few
individual contained the transgene only
after three generations while in laboratory
strains, it was persistent for over one fifty
generations.
54. What are the
limitations of SIT?
• Geography. The eradication zone must have either natural
barriers to prevent the immigration of the target pest
from outside.
• Economics. Cost of rearing, sterilizing, and releasing a
large numbers of insects can be very high.
• Desirability of sterile males. The lab-reared and
sterilized males must be equally or more competitive than
the native males in mating with the native females. They
may become less desirable after many generations and
need renewal.
55. • Knowledge about the pest. reproductive
behavior, population dynamics, dispersal, and
ecology of the insect.
• Accurate estimation of the native population
density
• Timing. The development of the lab-reared
colony must be synchronous with that of the
wild population.
• Resistance. Native females may be able to
recognize and refuse to mate with sterile
males.
56. FUTURE PROSPECTS
• Transgenic insect approach will help
to control harmful insects and create
beneficial insects.
• Creation of transgenic insects with
increase fitness.
• Biosafety research on transgenic
insect has to gain important in
international symposia.
• Risk assessment guidelines require
more clarification.
57. Conclusion:
SIT has been, and continues to be, a hotbed of
genetic innovation. transgenic technology
offers a much wider spectrum of advances in
genetic tools for SIT, from heritable marking
to alternative methods for sterilisation. it is,
increase the range of pest species that can be
targeted by this environmentally friendly,
species-specific method of control.
Editor's Notes
Zanzibar – which consists of a number of islands off east africa
tsetse transmitted trypanosomiasis is called sleeping sickness.
Human African trypanosomiasis, sleeping sickness is a parasitic disease of people and animals, caused by protozoa of the species Trypanosoma brucei and transmitted by the tsetse fly.
covering about 36 countries and 60 million people. It is estimated that 50,000 to 70,000 people are currently infected, the number having declined somewhat in recent years.[3] It is believed that many cases go unreported. About 48,000 people died of it in 2008
but there are no large-scale SIT programs in operation today against any mosquito