This document provides an overview of transgenic organisms. It begins with definitions of key terms like transgene, genome, plasmid, and restriction enzyme. It then discusses the history of transgenic research, including the first genetically modified organism created in 1973 and the first transgenic animals. The main methods used to produce transgenic animals are described as DNA microinjection, embryonic stem cell-mediated gene transfer, and retrovirus-mediated gene transfer. Current applications of transgenic organisms are outlined, such as glowing fish and insects used for pest control. The document concludes by discussing the importance of transgenic organisms in medicine, agriculture, and industry.
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
This presentation gives a comprehensive detail of transgenic animal, processes involve in the production of transgenic animal and also highlights several benefits of transgenic animal
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
It's include all the details about the transgenic technology.all the techniques like micro injection,SCNT,pro nuclear injection method.It include all the Transgenic mice bird and fish.
Introduction
Definition
History
Why are the transgenic animals being produced
Transgenic mice
Mice: as model organism
Methods of creation of transgenic mice
knock-out mice
Application of transgenic mice
Conclusion
References
It's include all the details about the transgenic technology.all the techniques like micro injection,SCNT,pro nuclear injection method.It include all the Transgenic mice bird and fish.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
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Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
2. CONTENTS
● Meaning and basic
understanding
● History
● Production methods and
techniques
● Present scenario
● Importance of transgenic
organisms and their
applications
● Impacts on various factors
3. Definition
Transgenesis (also called genetic
modification) is the process of
introducing a gene (transgene)
from one organism to genome of
another organism.The aim is that
the resulting transgenic organism
will express the gene and exhibit
some new property or
characteristics.
4. Basic terms to know
● Transgene - foreign gene or genetic material that has been
transferred naturally or using genetic engineering techniques
from one organism to another.
● Genome - the complete set of genes or genetic material
present in a cell or organism.
● Plasmid - a small, extrachromosomal DNA and can replicate
independently
● Restriction enzyme - a protein that recognises a specific,
short nucleotide and cuts DNA only at specific site known as
restriction site or target sequence.
5. HISTORY
The first GMO was created in 1973 Stanley
N.Cohen and Herbert Boyer. Demonstrating the
creation of a functional organism that combined
and replicated genetic information from different
species.
The first transgenic animals were mice created by
Rudolf Jaenisch in 1974. Jaenisch successfully
managed to insert foreign DNA into the early stage
mouse embryos;the resulting mice carried the
modified gene in all their tissues.
● Injecting leukemia genes to early mouse
embryos using a retrovirus vector, proved the
genes integrated not only to the mice
themselves, but also their progeny.
6.
7. The first two commercially prepared products from DNA recombinant
technology were insulin and human growth hormone,both of which were
cultured in E.coli bacteria.
8. Production methods and techniques
There are 3 principal methods used for production of transgenic animals:
❏ DNA Microinjection
❏ Embryonic stem cell-mediated gene transfer
❏ Retrovirus-mediated gene transfer
9. Microinjection.
Microinjection is a
technique of delivering
foreign DNA into a living
cell (a cell, egg, oocyte,
embryos of animals)
through a glass
micropipette. One end of
a glass micropipette is
heated until the glass
becomes somewhat
liquified. It is quickly
stretched which forms a
very fine tip at the heated
end.
10. Embryonic-stem cell-
mediated gene transfer
This method involves prior
insertion of the desired DNA
sequence by homologous
recombination into an in vitro
culture of embryonic stem
(ES) cells. ... These cells are
then incorporated into an
embryo at the blastocyst stage
of development. The result is a
chimeric animal.
Chimera:A genetic chimerism or chimera is a
single organism composed of cells with more than
one distinct genotype.
11. This gene transfer is
mediated by means of a
carrier or vector, generally a
virus or a plasmid.
Retroviruses are commonly
used as vectors. The killed
virus is replication defective.
The virus gene is replaced
with trans-gene. The trans-
gene is delivered to the host
cell by transfection (gene
therapy)
Retrovirus mediated gene
transfer
12. In present world we use transgenic organisms a lot more than we realize or pay attention. Some of
modern uses are mentioned as :
Transgenic pets: Glow-in-dark fish
Ever have one of those groovy posters that glows under a black light? Well, move that black light over to the
aquarium — there’s a new fish in town. Originally derived from zebrafish, a tiny, black-and-white–striped native of
India’s Ganges River, these glowing versions bear a gene that makes them fluorescent. The little, red, glow-in-the-
dark wonders (referred to as GloFish) are the first commercially available transgenic pets.
Zebrafish are tried-and-true laboratory veterans-they even have their own scientific journal! Developmental
biologists love zebrafish because their transparent eggs make it simple to observe development. Geneticists use
zebrafish to study the functions of all sorts of genes, many of which have direct counterparts in other organisms,
including humans. And genetic engineers have taken advantage of these easy-to-keep fish, too; scientists in
Singapore saw the potential to use zebrafish as little pollution indicators. The Singapore geneticists used a gene
from jellyfish to make their zebrafish glow in the dark. The action of the fluorescent gene is set up to respond to cues
13. The transgenic zebrafish then provide a quick and easy to read signal: If they glow, a pollutant
is present.
14.
15. Another potential use for transgenic insects
would be to release of millions of
transgenically infertile bugs that attract the
mating attentions of fertile ones. The matings
result in infertile eggs, reducing the
reproduction of the target insect population.
This is an especially appealing idea when
used to combat invasive species that can
sweep through crops with economically
devastating results.
16. Transgenic bacteria
Bacteria are extremely amenable to transgenesis. Unlike other transgenic organisms,
genes can be inserted into bacteria with great precision, making expression far easier to
control. As a result, many products can be produced using bacteria, which can be grown
under highly controlled conditions, essentially eliminating the danger of transgene escape.
Many important drugs are produced by recombinant bacteria, such as
insulin for treatment of diabetes, clotting factors for the treatment of
hemophilia, and human growth hormone for the treatment of some
forms of short stature. These sorts of medical advances can have
important side benefits as well:
17. ● Transgenic bacteria can produce much greater volumes of proteins than traditional methods.
● Transgenic bacteria are safer than animal substitutes, such as pig insulin, which are slightly
different from the human version and may therefore cause allergic reactions.
18. Transgenic plants
Plants are really different from animals, but not in the way you may think. Plant cells are totipotent,
meaning that practically any plant cell can eventually give rise to every sort of plant tissue: roots,
leaves, and seeds. When animal cells differentiate during embryo development, they lose their
totipotency forever (but the DNA in every cell retains the potential to be totipotent). For genetic
engineers, the totipotency of plant cells reveals vast possibilities for genetic manipulation.
Much of the transgenic revolution in plants has focused on moving genes from one plant to
another, from bacteria to plants, or even from animals to plants. Like all transgenic organisms,
transgenic plants are created to achieve various ends, including nutritionally enhancing certain
foods (such as rice) or altering crops to resist either herbicides used against unwanted competitor
plants or the attack of plant-eating insects.
19.
20. IMPORTANC
E
Medical importance
Transgenesis has contributed a lot in field of medicine as it is used to
discover some drugs,to see the impacts of some drug or vaccine and so
on.Their uses in medicine are unlimited.
❏ GENE THERAPY OF HUMANS:
Human gene therapy is the process in which by using the technique of
gene therapy in which healthy genes are inserted in the person’s body
in place of defective genes.Defective genes do not function properly
and cause diseases in the body.plasmids and vectors are used to
carry healthy genes.Transgenic animals could play vital role in the
treatment of almost 5000 genetic disorders.For example, the
scientists of Finland have developed a calf which carries a gene.The
gene is responsible for making a substance which provides the
growth of RBCs in humans.
21. ❏ XENOTRANSPLANTATION:
There are a lot of people in the world who die every year because of
organ failure.Some people have problems in their kidneys,, some in
lungs etc. In UK alone , almost 5000 organs are needed for the
patients. Scientists have successfully done experiments in pigs and
have developed human organs.Though their is a protein in pig which
cannot be accepted by human immune system but scientists are
working on this problem and are trying to replace this protein with
human protein.
❏ PHARMACEUTICALS:
Scientists have produced insulin, growth hormones and blood clotting
factors in the transgenic animals.These are obtained from cow’s milk
and are very useful for human health.Blood clotting factor can be
beneficial for
Patients of haemophilia because they lack the ability to clot the blood.
Similarly milk is being obtained from goats or sheep through
transgenesis to treat diseases like cystic fibrosis and phenylketonuria.
22. Agricultural Importance:-
❏ Breeding:-
It is the culture of all farmers to breed animals selectively so that healthy animals can be
obtained that have the traits required by the farmers, for example, increased amount of milk
and healthy meat. Though traditional breeding is also common but it gives slow results as it is
a time consuming method. Molecular biology has made it possible to develop such
techniques which give better results in a short time. It is also beneficial for farmers in the
sense that farmer can get higher yield in a short time.
❏ Disease Resistance:-
Scientists are doing research on making such animals which are disease resistant. Diseases
like influenza can cause damage to the animal body that is why genes are inserted in pigs
which show resistant against this disease. farm animals are more susceptible to influenza as
they live in groups and disease spreads from one animal to the other.
❏ Quality:-
Quality is another factor which farmers want to increase. Transgenic cows are able to produce
milk which has more nutritional value than an ordinary cow's milk. Some farmers tried to use
growth hormones in animals but they didn't succeed in getting the desired results.
23. Industrial Importance:-
Two scientists of Canada have successfully inserted spider genes into goats
which are lactating. now it is possible that along with the milk production,
goats will also produce silk which is a light flexible material used to make
army uniforms, tennis rackets and medical microsutures. For the safety of
chemicals, scientists have produced toxicity sensitive transgenic animals.
Many proteins have produced by using transgenic animals which in turn
convert into enzymes to perform different functions in the body.