- Systemic Acquired Resistance (SAR) is a plant defense mechanism that confers long-lasting protection against a broad spectrum of pathogens. It involves the accumulation of pathogenesis-related (PR) proteins in response to salicylic acid (SA) signaling.
- SAR is triggered by initial infection at a local site and protects the entire plant. It provides an alternative to toxic chemicals for controlling plant diseases in a sustainable way.
- Key events in SAR include the production of SA at the infection site, transmission of the SAR signal systemically, and accumulation of PR proteins that contribute to broad-spectrum resistance against future infections.
Gene for gene system in plant fungus interactionVinod Upadhyay
MOLECULAR CHARACTERIZATION OF GENE FOR GENE SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF PLANT PATHOGENS
Pathogenesis-related proteins (initially named “b” proteins) were discovered in tobacco leaves
hypersensitively reacting to TMV by two independently working groups (Van Loon and Van Kammen,
1970; Gianinazzi et al., 1970)
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
Systemic Acquired Resistance (SAR) and it’s Significance in Plant Disease Ma...Ankit Chaudhari
Systemic Acquired Resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms and pests. Presently disease control is largely based on the use of hazardous chemicals viz., fungicides, bactericides and insecticides for either direct or indirect disease management. The hazardous natures of the products on the environment, human and animal health strongly necessitates the search for new safer means of disease control. SAR have high potential to diminish the use of toxic chemicals in the agriculture and has emerged as an alternative, non-conventional, non-biocidal and eco-friendly approach for plant protection and hence for sustainable agriculture. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance.
Plant - Pathogen Interaction and Disease DevelopmentKK CHANDEL
Plant diseases are the result of infection by any living organisms that adversely affect the growth, development, physiological functioning and productivity of a plant, manifesting outwardly as visible symptoms.
Signal transduction in plant defence responsesrkravikirankt
Plant respond to the attack of diseases by triggering various bio-molecules insider their system to combat the infection and establishment of the pathogens. these response operate in specified pathways mediated by many enzymes starting from the infection site to the nucleus which together constitute the signal transduction pathway.
Gene for gene system in plant fungus interactionVinod Upadhyay
MOLECULAR CHARACTERIZATION OF GENE FOR GENE SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF PLANT PATHOGENS
Pathogenesis-related proteins (initially named “b” proteins) were discovered in tobacco leaves
hypersensitively reacting to TMV by two independently working groups (Van Loon and Van Kammen,
1970; Gianinazzi et al., 1970)
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
Systemic Acquired Resistance (SAR) and it’s Significance in Plant Disease Ma...Ankit Chaudhari
Systemic Acquired Resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms and pests. Presently disease control is largely based on the use of hazardous chemicals viz., fungicides, bactericides and insecticides for either direct or indirect disease management. The hazardous natures of the products on the environment, human and animal health strongly necessitates the search for new safer means of disease control. SAR have high potential to diminish the use of toxic chemicals in the agriculture and has emerged as an alternative, non-conventional, non-biocidal and eco-friendly approach for plant protection and hence for sustainable agriculture. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance.
Plant - Pathogen Interaction and Disease DevelopmentKK CHANDEL
Plant diseases are the result of infection by any living organisms that adversely affect the growth, development, physiological functioning and productivity of a plant, manifesting outwardly as visible symptoms.
Signal transduction in plant defence responsesrkravikirankt
Plant respond to the attack of diseases by triggering various bio-molecules insider their system to combat the infection and establishment of the pathogens. these response operate in specified pathways mediated by many enzymes starting from the infection site to the nucleus which together constitute the signal transduction pathway.
Systemic acquired resistance (SAR): A novel strategy for plant protection.mohd younus wani
Exclusive reliance on pesticides, fungicides and herbicides resulted in pesticide and herbicide, resistance, pest resurgence, residues, environmental pollution. Plants have developed various resistance mechanisms to help them adapt to pathogen and insect attack (Jones and Dangl, 2006). Systemic acquired resistance (SAR) is a form of induced resistance that is activated throughout a plant after being exposed to elicitors from virulent, avirulent, or nonpathogenic microbes, or artificial chemical stimuli such as chitosan or salicylic acid (SA) (Gozzo and Faoro, 2013).It is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which contribute resistance to the plants.
They can be used as fungicide alternative without any threat of developing resistance and being safe and ecofriendly (Najar et al, 2010). The elicitor, β-Amino butyric acid induces greater systemic resistance to mulberry in addition to enhancement in biochemical parameters and NPK contents of mulberry leaves (Mazal, 2014).Therefore, in order to control the diseases of mulberry without adverse effect on environment, humans and silkworms health attention needs to be given to promote SAR chemicals. A model needs to be framed to promote the use of these chemicals in order to make sericulture more profitable. This is an ecofriendly approach of disease and pest management. The chitinase genes of mulberry induced by insect wounding and fungal infection, suggesting that these chitinases help the mulberry plant to cope with the challenges from insects and fungi (Wang et al., 2015). Jasmonic acid (JA) is an important plant defense signal mediating resistance to herbivores.
Presently disease control is largely depends on the use of fungicides, bactericides and insecticides. The hazardous nature of these chemicals on the environment, human health and silkworm strongly necessitates the search for new, harmless means of disease control.Induced resistance like SAR can diminish the use of toxic chemicals for disease control and thus could be proposed as an alternative, non-biocidal, ecologically-friendly approach for plant protection and hence for sustainable Sericulture. Induced resistance is increased expression of Natural defense mechanisms against different pathogens provoked by external factors of various types. Systemic acquired resistance (SAR) is a "whole-plant" resistance response and can be distinguished from other disease resistant responses by both the spectrum of pathogen protection and the associated changes with gene expression.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
This pdf is about the Schizophrenia.
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2. SAR
(SYSTEMIC ACQUIRED
RESISTANCE)
SUBMITTED TO:-
DR.SURJYA KANTA BEURA SUBMITTED BY :-
PROFESSOR AND HEAD. UDAYENDU BARIK
DEPT. OF PLANT PARHOLOGY M.Sc.(ag) Ist year
OUAT,BHUBANESWAR admsn. no – 18122l10
dept. of plant pathology
ouat,bhubaneswar
3. CONTENT
Introduction
Basics of Systemic Acquired Resistance (SAR)
History of SAR
Importance of SAR in plant diseases management
Mechanisms of SAR
PR Proteins in SAR
Conclusion
4.
5.
6. Systemic Acquired Resistance (SAR) is a plant defense mechanism of induced
defense that confers long-lasting protection against a broad spectrum of
microorganisms & pest.
Presently disease control is largely based on the use of hazardous
chemicals viz., fungicides, bactericides and insecticides for either direct or
indirect disease management.
The hazardous natures of the products on the environment, human and animal
health strongly necessitates to search for new safer means of disease control.
SAR have high potential to diminish the use of toxic chemicals in the
agriculture and has emerged as an alternative, non-conventional, non-biocidal
and eco-friendly approach for plant protection and hence for sustainable
agriculture.
SAR requires the signal molecule salicylic acid (SA) and is associated
with accumulation of pathogenesis-related proteins, which are thought to
contribute to resistance (Dube, 2017).
INTRODUCTION
9. What is induced resistance ???
The induced resistance can be defined as an increased expression of
natural defense mechanisms of plants against different pathogens.
LAR ISR SAR
10. o A distinct signal transduction pathway that plays an important role in the
ability of plants to defend themselves against pathogens…..
o Significant phenomenon recognized by Chester in 1933.
o Concept of SAR proposed by Ronald Ross in 1961.
o Infection of plants with necrotizing pathogens (causing HR) often results in
enhanced resistance to subsequent infections by a variety of bio-tropic
pathogens.
o SAR requires the signal molecule salicylic acid (SA) and is associated with
accumulation of Pathogenesis Related proteins, which are thought to
contribute to resistance.
o Resistance triggered in the plant during its life time is Acquired Resistance.
Local (LAR) confined to few cells or tissues
Systemic (SAR) having been moved through out the plant
SAR (Systemic AcquiredResistance)
12. History of SAR
Year Name Contribution
1901 Ray & Beauverie The natural phenomenon of resistant in response to pathogen infection
or plant immunity
1932 Carbonne &
Kalaljev
Showed that acquired resistant also depends on the general fitness of the
host
1933 Chester Documented the idea of PhysiologicalAcquired Immunity
1961 A. F. Ross Published the first systemic study of SAR
He coined the term “Systemic acquired resistance”
1970 V
an et al. Showed that viral infection of tobacco induced the accumulation of a distinct
set of proteins, called pathogenesis-related proteins (PR proteins)
1979 Ray White observed that PR protein accumulation and resistance to TMV could be
induced by treatment of tobacco with salicylic acid (SA), aspirin (acetyl SA), or
benzoic acid
1991 Ward et al. Studied steady-state mRNA levels from at least nine families of genes were
shown to be coordinately induced in uninfected leaves of inoculated plants;
these gene families are now known as SAR genes
1993 Gaffney et al A requirement for SA as an endogenous signal for SAR was proven with using
a bacterial gene, nahG, encoding salicylate hydroxylase, which removes SA by
conversion tocatechol
13. o SA dependent
o Necrosis reaction
present
o Signaling molecules SA
o Less elastic
o Against biotrophs
o Continuous irritation is
not required
o PR-proteins involved
o SA independent
o Necrosis reaction
absent
o Signaling molecules
JA, Ethylene
o More elastic
o Against necrotrophs
and insects
o Continuous irritation
is required
o Defense genes
involved
SAR ISR
14. Importance of SAR in plant diseases management
o SAR can also be transmitted to the next generation progeny.
o Its provides a broad-range resistance against
pathogens.
o Leads to pathogenesis-related (PR) gene expression.
fungal, bacterial and viral
o Its ability to immunize susceptible plants implies that genetic potential
for resistance is in all types of plants.
o The significant practical aspects of SAR is the discovery of chemical inducers of
plant defense.
o New generation fungicides act as a plant defense system. (rather than killing
pathogen.) ICGA-245704, a benzothiadiazole (BTH) (Alfa-Aesar) & Dichloro-
isonicotinic acid (DCINA) compound (plant-activator) switches SAR in plant.
15. Signal generation and transmission In SAR
Ideal Charactristics of Transported Signal:
o Induce a defensive response
o Produced or released at the site of attack
o Translocated from the attacked to the systemic tissue
o Accumulate in the systemic tissue before resistance expression takes place
(Choi & Hwang, 2011)
17. Pathogenesis related proteins (PRs) are assigned an important role
in plant defense against pathogenic constraints and in general
adaptation to stressful environment.
These proteins are accumulated 7-10 days after infection and indicate
the attainment of SAR.
It is accumulated in the intercellular spaces (first line of defence) and
vacuole (second line of defence by lytic enzyme).
Pathogenesis-Related (PR) Proteins in SAR
18. Accumulation of PR Proteins in SAR
(Pieterse and Van Loon, 2004)
Cytoplasma
19. Low-
molecular
Proteins
(5-75 ) kDa
Stable at low
pH (< 3)
Thermostable
& Highly
resistant to
proteases
Contains four α-
helices and β-
strands
arranged
antiparallel
between helices
Established in all
plant organs–
leaves, stems,
roots, flowers
Feature:
Antifungal,
Antibacterial,
Insecticidal &
Antiviral action
Biochemical
and structural
characteristics
of PR Proteins
20. Classification and Properties of families of PR proteins
Family Type member Properties Targeted Pathogen Site
PR-1 Tobacco PR-1a Antifungal Active against Oomycetes
PR-2 Tobacco PR-2 β-1,3-Glucanase Cell wall Glucan of fungi
PR-3 Tobacco P, Q class (I,II, IV,V,VI,VI)
Chitinase Cell wall chitin of fungi
PR-4 Tobacco ‘R’ Chitinase class I,II Cell wall Chitin of fungi
PR-5 Tobacco S Thaumatin-like, Active against Oomycetes
PR-6 Tomato Inhibitor I Proteinase-inhibitor Active on Nematodes + Insect
PR-7 Tomato P69 Endoproteinase Microbial cell wall dissolution
PR-8 Cucumber chitinase Chitinase class III Cell wall Chitin of fungi +
Mucopeptide wall of bacteria
PR-9 Tobacco ‘lignin-
forming peroxidase’
35
Peroxidase Antimicrobial activity by
catalyzing oxidative cross-linking
protein and phenolic in cell wall
leading to physical barrier
AND MANY MORE FAMILIES………..
22. SIGNALS FOR SYSTEMIC ACQUIRED RESISTANCE
Electrical
Signals
Reactive
Oxygen Species
Lipid-Based Signal
Molecule
Natural Organic
Compounds
Inorganic
Compounds
oSalicylic Acid (SA)
o Jasmonic Acid (JA)
oMethyl ester JA (MeJA) o
Methyl ester SA (MeSA) o
Ethylene
o Systemin
o Riboflavin
o Phosphate salts
o Silicon
o Synthetic compounds
BABA (β-aminobutyric acid)
INA (2,6 – dichloroisonicotinic acid )
BTH/ ASM (Benzo-(1, 2, 3)-thiadiazole-7-
carbothioic acid S-methyl ester )
o H2 O2
o DPI (Diphenylene iodonium)
o dir 1
o LTPs
o eds 1
o pad 4
o SABP 2
o Pin2 mRNA
(Sticher, 1997)
23. CONCLUSION
SAR is effective against a broad range of pathogens and parasites,
including fungi, bacteria and viruses.
Salicylic acid is not a translocated signal responsible for inducing SAR
but is required in signal transduction.
Methyl salicylate, lipid signaling, peptide signaling, Green-leaf volatiles
are the transported signals in SAR.
SA, MeSA, JA, BTH/ASM, INA, BABA and H2O2 plays important role in
building broad-spectrum disease resistance against many plant diseases.
Over expression of an essential regulatory gene (NPR 1) leads to the
generation of broad-spectrum disease resistance in plant which is also
known as systemic acquired resistance.