Fungi are eukaryotic microorganisms which are heterotrophic and essentially aerobic with limited anaerobic capabilities. Fungi synthesize lysine by the L-αadipic acid biosynthetic pathway. They possess chitinous cell walls, plasma membranes containing ergosterol, 80SrRNA and microtubules composed of tubulin. Fungi grow as yeasts, molds (filamentous) or a combination of both (i.e. dimorphism).
Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Oxidase Test Microbiology - Principle, Procedure, Limitations, Results, QC - in lab #Oxidase Test
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
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Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Oxidase Test
#Medical
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Capsule is an layer around the bacteria cell which gives bacteria the protection and pathogenicity. Staining such an layer is difficult with the normal stains so it is necessary to stain the background and the cell itself which makes the capsule appear colourless.
Oxidase Test Microbiology - Principle, Procedure, Limitations, Results, QC - in lab #Oxidase Test
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks...
If you like my video
#like
#comment
#subscribe my channel
don't forget to subscribe my channel
Qualification
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Oxidase Test
#Medical
#Microbiology
# malayalam lecturer
#Mallu Medicos Lounge
#MalluMedicosLounge
#MLT
Medically Important Candida albicans.pptxNawangSherpa6
The Presentation here is about Medically important Candida species. How does it infect the Human host? What are it's clinical manifestations and How can we diagnose for their infection and potential application for other studies.
This seminar consisits of description of various bacterial diseases along with their oral manifestations,diagnosis and treatment.an addition of suitable case reports for better understanding and associated disorders
Fungi were found by Heinrich Anton de Bary in 1858.
Most fungi cause skin or cosmetic infections while bacteria & viruses cause fatal diseases.
Organ transplantation, Immunosuppressive drugs,Anticancer drugs, Broad-spectrum antimicrobials ,HIV-disease leads to Immunosuppression causing Opportunistic Fungal Infections
Fungal Infection (pharmacology and rehab)KarimFarhat7
I'll provide a brief overview of fungal infections, covering their causes, symptoms, diagnosis, treatment, and prevention. If you have specific questions or need more details on any aspect, feel free to ask.
---
**Fungal Infections: Understanding the Basics**
Fungal infections, also known as mycoses, are caused by various types of fungi that can invade the body and lead to a range of health issues. Fungi are organisms similar to plants but lacking chlorophyll, making them unable to produce their own food through photosynthesis. Instead, they obtain nutrients from other organic matter.
**1. Types of Fungi Responsible for Infections:**
Fungal infections can be caused by different types of fungi, including yeasts, molds, and dermatophytes. Common fungal species that cause infections in humans include Candida, Aspergillus, and dermatophytes like Trichophyton.
**2. Causes and Risk Factors:**
Fungal infections can occur when fungi enter the body through various means, such as inhalation, ingestion, or direct contact with the skin. Certain factors increase the risk of fungal infections, including weakened immune systems, underlying health conditions, prolonged use of antibiotics, and environmental exposure.
**3. Common Types of Fungal Infections:**
- **Candidiasis:** A yeast infection caused by Candida species, often affecting areas like the mouth, genital region, and skin folds.
- **Aspergillosis:** Caused by the Aspergillus species, commonly affecting the respiratory system.
- **Ringworm:** A dermatophyte infection that affects the skin, nails, or scalp.
**4. Symptoms and Clinical Presentation:**
Symptoms of fungal infections vary depending on the type and location of the infection. Common signs include itching, redness, swelling, rashes, and in some cases, systemic symptoms like fever and fatigue.
**5. Diagnosis:**
Accurate diagnosis is crucial for effective treatment. Healthcare professionals may use various methods, including laboratory tests, imaging studies, and clinical examination, to identify the type and extent of the fungal infection.
**6. Treatment Options:**
Treatment of fungal infections often involves antifungal medications. These can be topical, oral, or intravenous, depending on the severity and location of the infection. Additionally, addressing underlying health conditions and improving immune function may be crucial for successful treatment.
**7. Prevention Strategies:**
Preventing fungal infections involves adopting good hygiene practices, avoiding contact with contaminated surfaces, and addressing risk factors such as diabetes or immunosuppression. Proper antifungal prophylaxis may be recommended in specific situations.
**8. Emerging Research and Therapies:**
Ongoing research aims to improve our understanding of fungal infections and develop more effective therapies. This includes exploring new antifungal agents, understanding the role of the microbiome, and developing vaccines.
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
An enzyme is a substance that acts as a catalyst in living organisms, regulating the rate at which chemical reactions proceed without itself being altered in the process. The biological processes that occur within all living organisms are chemical reactions, and most are regulated by enzymes
The aqueous humour is a transparent, watery fluid similar to plasma, but containing low protein concentrations. It is secreted from the ciliary epithelium, a structure supporting the lens
The cornea is the transparent front part of the eye that covers the iris, pupil, and anterior chamber. The cornea, with the anterior chamber and lens, refracts light, with the cornea accounting for approximately two-thirds of the eye's total optical power.
Lens is a transparent, biconvex, crystalline structure placed between iris and the vitreous in a saucer-shaped depression, the patellar fossa. The lens is a crystalline structure that is avascular and is devoid of nerves and connective tissue
It consists of three distinct part:
Lens capsule
Anterior lens epithelium, and
Lens substance or lens fibres
Small amounts of vitamins are required in the diet to promote growth, reproduction, and health. Vitamins A, D, E, and K are called the fat-soluble vitamins, because they are soluble in organic solvents and are absorbed and transported in a manner similar to that of fats.
Water soluble vitamins include Vitamin C and the vitamin B complex: thiamin (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), Vitamin B6, biotin (B7), folic acid (B9), Vitamin B12. Vitamin A in its Beta-Carotene form is also water-soluble.
The tear film is a complex mixture of substances secreted from multiple sources on the ocular surface, including the lacrimal gland, the accessory lacrimal glands, the meibomian glands, and the goblet cells.
A picornavirus is a virus belonging to the family Picornaviridae, a family of viruses in the order Picornavirales. Vertebrates, including humans, serve as natural hosts. Picornaviruses are nonenveloped viruses that represent a large family of small, cytoplasmic, plus-strand RNA viruses with a 30-nm icosahedral capsid.
Poxviruses are brick or oval-shaped viruses with large double-stranded DNA genomes. Poxviruses exist throughout the world and cause disease in humans and many other types of animals. Poxvirus infections typically result in the formation of lesions, skin nodules, or disseminated rash.
Leptospirosis is a bacterial disease that affects humans and animals. It is caused by bacteria of the genus Leptospira. In humans, it can cause a wide range of symptoms, some of which may be mistaken for other diseases. Some infected persons, however, may have no symptoms at all.
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS).
Treponema is a genus of spiral-shaped bacteria. The major treponeme species of human pathogens is Treponema pallidum, whose subspecies are responsible for diseases such as syphilis, bejel, and yaws.
Haemophilus is the name of a group of bacteria. There are several types of Haemophilus. They can cause different types of illnesses involving breathing, bones and joints, and the nervous system. One common type, Hib (Haemophilus influenzae type b), causes serious disease. It usually strikes children under 5 years old
Moraxella is a genus of Gram-negative bacteria in the Moraxellaceae family. It is named after the Swiss ophthalmologist Victor Morax. The organisms are short rods, coccobacilli, or as in the case of Moraxella catarrhalis, diplococci in morphology, with asaccharolytic, oxidase-positive, and catalase-positive properties
Pseudomonas is a type of bacteria that can cause infections. Pseudomonas is a common genus of bacteria, which can create infections in the body under certain circumstances. There are many different types of Pseudomonas bacteria
Neisseria gonorrhoeae is the obligate human pathogen that causes the sexually transmitted disease (STD) gonorrhea. This Gram-negative diplococci/gonococci does not infect other animals or experimental animals and does not survive freely in the environment. The gonococcal infection occurs in the upper or lower tract, pharynx, ophthalmic area, rectum, and bloodstream. During the 1980’s gonorrhea was also referred to as “the clap” when public awareness was quite minimal. This was one of the venereal diseases prostitutes hoped to contract since it resulted in infertility by pelvic inflammatory disease (PID). As documentation, diagnostic testing, and public awareness improved, there has been a decline in incidence reports, however, it is still considered a very common infectious disease.
Meningococci are a type of bacteria that cause serious infections. The most common infection is meningitis, which is an inflammation of the thin tissue that surrounds the brain and spinal cord. Meningococci can also cause other problems, including a serious bloodstream infection called sepsis. In its early stages, you may have flu-like symptoms and a stiff neck. But the disease can progress quickly and can be fatal. Early diagnosis and treatment are extremely important. Lab tests on your blood and cerebrospinal fluid can tell if you have it. Treatment is with antibiotics. Since the infection spreads from person to person, family members may also need to be treated.
A vaccine can prevent meningococcal infections.
Diphtheria is an infection caused by the bacterium Corynebacterium diphtheriae. Diphtheria causes a thick covering in the back of the throat. It can lead to difficulty breathing, heart failure, paralysis, and even death. CDC recommends vaccines for infants, children, teens and adults to prevent diphtheria. The presentation consists of basic concepts regarding the bacteria and its infection. It has explanation in detail about signs and symptoms of Diptheria
Contraindications, Adverse reactions and ocular nutritional supplementsArun Geetha Viswanathan
utritional supplements comprise a great deal of the products available over the counter in most pharmacies. Although most vitamin supplements are relatively harmless—except for the fat soluble ones A, D, E, and K—they are not the only supplements available to patients. Some of these other, non-vitamin supplements can actually be harmful to patients and often they have been proven to be ineffective. This doesn’t mean that patients will stop taking them though, which in turn leaves the potential for contraindications of nutritional supplements with prescription-based drugs wide open.
Ageing is a gradual process that takes place over many decades. Most theories of ageing relate to impaired DNA replication and loss of cell viability and hence the viability of the body’s organs. Ageing is often accompanied by socioeconomic changes that can have a great impact on the nutritional needs and status of elderly individuals. The incidence of disability increases with ageing, with over a third of the elderly population limited by chronic conditions and unable to carry on normal daily living activity
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
1. Fungi
Fungi are eukaryotic
microorganisms which are
heterotrophic and essentially
aerobic with limited
anaerobic capabilities. Fungi
synthesize lysine by the L-
αadipic acid biosynthetic
pathway. They possess
chitinous cell walls, plasma
membranes containing
ergosterol, 80SrRNA and
microtubules composed of
tubulin. Fungi grow as yeasts,
molds (filamentous) or a
combination of both (i.e.
dimorphism).
2. Common features of fungi
• Fungi are not plants or animals, they form a separate
group of higher organisms, distinct from both plants and
animals, which differ from other groups of organisms in
several major respects:
1. Fungal cells are encased within a rigid cell wall, mostly
composed of chitin and glucan.
• These features contrast with animals, which have no cell walls,
and plants, which have cellulose as the major cell wall
component.
3. Common features of fungi
2. Fungi are heterotrophic.
• This means that they are lacking in chlorophyll and cannot
make their organic food as plants can, through
photosynthesis.
• Fungi live embedded in a food source or medium, and
obtain their nourishment by secreting enzymes for
external digestion and by absorbing the nutrients that are
released from the medium.
• Some live on humans e.g. the cause ringworm infections.
Some live on insects, or plants.
3. Fungi are simpler in structure
• There is no division of cells into organs or tissues.
4. Common features of fungi
• Fungi are heterotrophic and essentially aerobic, with
limited anaerobic capabilities
• Fungi possess chitinous cell walls, plasma
membranes containing ergosterol, 80S rRNA, and
microtubules composed of tubulin.
5. Structure
Fungi
Yeast Filamentous Dimorphic
Yeasts are
single-celled
forms that
reproduce by
budding
Also called as
molds, form
multicellular
hyphae
Dimorphic
fungi grow as
yeasts or
spherules in
vivo and in
vitro at 37°C,
but as molds at
25°C.
6. Yeast: Candida
• Yeasts are fungi that grow as solitary cells that
reproduce by budding
• Candidiasis is a fungal infection due to any type of
Candida (a type of yeast).
• When it affects the mouth, it is commonly called
thrush
• When it affects the vagina, it is commonly called a
yeast infection
• More than 20 types of Candida can cause infection
with Candida albicans being the most common
7. 3 types
Mucosal candidiasis
• The mucosal surfaces primarily affected by
candidiasis are the oral cavity, esophagus, angles of
the mouth, and genitals (causing vulvovaginitis in
females, balanitis in males)
Cutaneous candidiasis
• Candidiasis on skin, nail and hair. Keratinase is
synthesized by fungus that mobilizes keratin
8. 3 types
Systemic candidiasis
• includes a spectrum of yeast infections caused by
different species of Candida.
• It is a serious infection that can affect the blood, heart,
brain, eyes, bones, or other parts of the body.
• most common form of this is when Candida enters the
bloodstream - candidemia.
• Signs of candidemia : fever and chills
• Diagnosis can be difficult, especially when the Candida
is not found in the bloodstream.
• Treatment usually includes consists of oral or
intravenous (IV) antifungal medications
9. Signs and symptoms
• In healthy persons, candidiasis is usually a localized infection of the
skin, fingernails or toenails, or mucosal membranes, including the
oral cavity and pharynx, esophagus, and the genitalia
• less commonly in healthy individuals, the gastrointestinal tract,
urinary tract, and respiratory tract are sites of candida infection.
• In immunocompromised individuals, Candida infections in the
esophagus occur more frequently and have a higher potential of
becoming systemic, causing a much more serious condition, a
fungemia called candidemia
10. Signs and symptoms
vaginal yeast infection
• vaginal itching
• swelling around the vagina
• burning during urination or sex
• pain during sex
• soreness
• redness
• rash
• vaginal discharge
Candidiasis of skin
• Rash
• redness and intense itching.
• become cracked and sore.
• Blisters and pustules may also
occur.
• Candida can also cause
infections in the nails, edges of
the nails, and corners of the
mouth.
11. Signs and symptoms
Oropharyngeal or mucosal
• Creamy white lesions on your
tongue, inner cheeks, and on the
roof of your mouth, gums and
tonsils
• Slightly raised lesions with a
cottage cheese-like appearance
• Redness, burning or soreness that
may be severe enough to cause
difficulty eating or swallowing
• Slight bleeding if the lesions are
rubbed or scraped
• Cracking and redness at the
corners of your mouth
• A cottony feeling in your mouth
• Loss of taste
• Redness, irritation and pain under
dentures (denture stomatitis)
• HIV/AIDS - Candida esophagitis
12. Diagnosis
• done either via microscopic examination or culturing.
For identification by light microscopy
• a scraping or swab of the affected area is placed on a
microscope slide. A single drop of 10% potassium
hydroxide (KOH) solution is then added to the specimen.
The KOH dissolves the skin cells, but leaves the Candida
cells intact, permitting visualization of pseudohyphae and
budding yeast cells typical of many Candida species.
13. Diagnosis
For the culturing method
• a sterile swab is rubbed on the infected skin surface.
The swab is then streaked on a culture medium.
• The culture is incubated at 37 °C for several days.
• The characteristics (such as morphology and colour)
of the colonies may allow initial diagnosis of the
organism causing disease symptoms
15. Treatment
• Clotrimazole
• Nystatin
• Fluconazole
• Voriconazole
• amphotericin B
• echinocandins.
• Intravenous fluconazole are
commonly used to treat
immunocompromised or
critically ill individuals.
Candidiasis is treated with antifungal medications
16. Filamentous fungi
Aspergillosis
• Infections caused by Aspergilli spp.
• ubiquitous in nature and play a significant role in the
degradation of plant material as in composting.
• rarely infect a normal host.
• Aspergillus fumigatus
• Aspergillus niger
• Aspergillus flavus
17. Symptoms
• Symptoms may vary from mild respiratory distress to alveolar
fibrosis.
• disseminate through the blood stream to cause widespread organ
damage.
• Symptoms include fever, chills, shock, delirium, seizures and blood
clots. The person may develop kidney failure, liver failure (causing
jaundice), and breathing difficulties.
• Fungus ball which is characteristically seen in the old cavities of TB
patients.
• The patients may cough up the fungus elements because the
organism frequently invades the bronchus.
• Chains of conidia can sometimes be seen in the sputum.
18. Diagnosis and treatment
• Chest X ray or CT Scan
• Microscopy
• Culture of fungus
Serology
• There is an excellent serological test for aspergillosis which is an
Immunodiffusion test. There may be 1 to 5 precipitin bands. Three or more
bands usually indicate increasingly severity of the disease. i.e., tissue
invasion.
Treatment
• Voriconazole and Amphotericin B.
19. Dimorphic fungi
Blastomycosis
• Caused by dimorphic Blastomyces dermatitidis
• survives in soil that contains organic debris and infects people
collecting firewood, tearing down old buildings or engaged in
other outdoor activities which disrupt the soil.
• a chronic granulomatous disease
• pulmonary and skin involvement is the most common,
frequently affects bone, prostate and other organs
20. Blastomycosis: symptoms
• The patient who presents with a complaint of respiratory
symptoms will frequently remark about loss of appetite,
loss of weight, fever, productive cough, and night sweats.
• an acute illness resembling bacterial pneumonia, with
symptoms of high fever, chills, a productive cough, and
pleuritic chest pain
• skin lesions
21. Blastomycosis: Diagnosis
• X-ray
• Culture: A culture of B. dermatitidis takes 7-10days to
grow at 37 degrees C
• Immunodiffusion test (precipitin). This requires 2 to 3
weeks to become positive
• Enzyme Immunoassay (EIA).
22. Blastomycosis: Treatment
• Amphotericin B remains is the drug of choice and
must be administered intravenously for several
weeks.
• Itraconazole is also being used in mild cases.
• Voriconazole is showing promise.
23. Fungal Eye Infections
Fungal infections can affect different parts of the eye.
• Keratitis is an infection of the clear, front layer of the eye (the
cornea).
• Endophthalmitis is an infection of the inside of the eye (the vitreous
and/or aqueous humor). There are two types of endophthalmitis:
exogenous and endogenous. Exogenous fungal endophthalmitis
occurs after fungal spores enter the eye from an external source.
Endogenous endophthalmitis occurs when a bloodstream infection
(for example, candidemia) spreads to one or both eyes.
24. Types of fungi that cause eye infections
Many of different types of fungi can cause eye infections.
1. Fusarium – a fungus that lives in the environment,
especially in soil and on plants
2. Aspergillus – a common fungus that lives in indoor and
outdoor environments
3. Candida – a type of yeast that normally lives on human
skin and on the protective lining inside the body called the
mucous membrane
25. Diagnosis and Treatment
• Microscope, cultured, Polymerase chain reaction
(PCR) and confocal microscopy
• Possible forms of treatment for fungal eye infections
include:
• Antifungal eye drops
• Antifungal medication given as a pill or through a vein
• Antifungal medication injected directly into the eye
• Eye surgery