This document discusses fungal culture methodology in the laboratory. It explains that growing fungi in pure culture is necessary for detection, isolation, identification, quantification, multiplication, and utilization of fungi. Pure cultures are also important for genetic resource conservation, development studies, industrial applications, and research programs. The document then describes several techniques for preserving fungal cultures, including periodic transfer, sterile water, sterile mineral oil, sterile soil/sand/silica gel, lyophilization, and their advantages and disadvantages. It emphasizes that preservation is important as fungal cultures are genetic resources.

1. FUNGAL CULTURE

2. Fungal culture methodology is the
foundation of fundamental and applied
reserches as well as the commercial
exploitation of fungi.

3. growth Purification preservation
What do we do to fungal cultures in laboratory?

4. Growing fungi in laboratory

5. Limiting factors for growth of fungi
•Temperature
•Light
•Aeration
•Humidity

6. Why pure culture is necessary?
• Detect
• Isolate
• Identify
• Quantify
• Multiply
• Utilize

7. Why preserve fungal culture?
Fungal culture are the genetic resource.
• Germplasm conservation
• Development studies
• Industrial applications
• Research programs

9. Preservation Techniques
1. Periodic transfer
2. Sterile mineral oil
3. Sterile water
4. Sterile soil or sand
5. Silica gel
6. L-drying
7. Lyphilization

10. Periodic transfer
Advantages
• Easy
• Inexpensive
• Required no special equipment
Disadvantages
• Labor- intensive
• May reduce variation
• May require contamination
• Time demanding

11. Sterile water
Advantages
• Clean Quick easy
• Less expensive
• Requires no special equipment
• Greater genetic stability
• Works for all fungi
Disadvantages
• Viability loss due to toxin trace in
water
• Viability upto 6 years

12. Sterile mineral oil
Advantages
• Easy
• Less expensive
• Require no special equipment
• Limit mite contaminations
• Viability reported upto 32 years
Disadvantages
• Does not work well for all fungi
• Retarded growth after retrieval
• Messy
• Risk of contamination at retrieval

13. Sterile Soil/Sand/Silica
Advantages
• Less expensive
• Technically simple
• Vigor maintained
• Can produce uniform inoculum
Disadvantages
• Work well for sporulating fungi
• Viability upro 8 years

14. Lyophilization
Advantages
• Long viability
• Ensured protection against
contamination
• Work well for all fungii
Disadvantages
• Expensive technique
• Complex technique
• Viability needs many trails

15. Culture accession process

16. Culture preservation process

17. Culture maintenance process

18. Thank you
By: Rahul
Guru Jambheshwar University of Science & Technology,
Hisar-Haryana