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FORENSIC ENTOMOLOGY
 Forensic entomology involves the application of the study of biology of
arthropods, including insects, arachnids, centipedes, millipedes, and
crustaceans to criminal or legal cases.
 Blood-feeding (haematophagous) insects
For example, Mosquitoes (Culicidae). A single mosquito can be used for
investigation (as a source of human DNA) even few days after taking blood.
 Necrophagous insects (corpse-eating)
For example, Flies, Beetles, Mites, Moths, Wasps, ants, and bees
FLIES
 Blow flies – Calliphoridae blue bottle fly, cluster fly,
greenbottles, or black blowfly
 Flesh flies – Sarcophagidae
 House fly – Muscidae
 Cheese flies – Piophilidae
 Coffin flies – Phoridae
 Lesser corpse flies – Sphaeroceridae
 Lesser house flies – Fanniidae
 Black scavenger flies – Sepsidae
 Sun flies - Heleomyzidae
 Black soldier fly - Stratiomyidae
 Phoridae – Humpbacked flies
BEETLES
 Rove beetles – Staphylinidae
 Hister beetles – Histeridae
 Carrion beetles – Silphidae
 Ham beetles – Cleridae
 Carcass beetles – Trogidae
 Skin/hide beetles – Dermestidae.
 Scarab beetles – Scarabaeidae
 Sap beetles – Nitidulidae
   Macrocheles feed on corpses in early stage of decomposition. Tyroglyphidae
and Oribatidae mites such as Rostrozetes feed on dry skin in the later stages of
decomposition.
MITES (Acari)
MOTHS
 Clothes-moths – (Tineidae) They are crepuscular and diurnal species. Moths
feed on mammalian hair during their larval stages and may forage on any hair
that remains on a body.
 They are amongst the final animals contributing to the decomposition of a
corpse.
 Some feed on the body, some are predatory, and eat the insects feeding on the
body.
 Bees and wasps have been seen feeding on the corpse body during the early
stages of their lifecycle.
WASPS, ANTS, AND BEES
FLIES: Calliphoridae
 Blow flies, blue bottle fly, cluster fly, greenbottles, black blowfly, carrion
flies, cluster flies.
 About 1,100 species of blow flies are known
Habitat
 India, Japan, China, Central America, and the Southern United States.
 The typical habitats for blow flies are temperate to tropical areas that provide a
layer of loose, damp soil and litter where larvae may thrive and pupate.
FOOD
 Adult blow flies are occasional pollinators, being attracted to flowers with
strong odors resembling rotting meat.
 Larvae of most species are scavengers of carrion and dung, and most likely
constitute the majority of the maggots found in such material.
Characteristics
 Adults are commonly shiny with metallic colouring, often with blue, green, or
black thoraces and abdomens.
 Antennae are three-segmented and aristate. The arista are plumose the entire
length, and the second antennal segment is distinctly grooved. Members of
Calliphoridae have branched Rs 2 veins, frontal sutures are present,
and calypters are well developed.
 Arrangement of hair like bristles located on the meron are used to differentiate
between members of this family. Having two notopleural bristles and a
hindmost posthumeral bristle located lateral to presutural bristle.
 Thorax has the continuous dorsal suture across the middle, along with well-
defined posterior calli. The postscutellum is absent or weakly developed. The
costa is unbroken and the subcosta is apparent on the insect.
Life cycle
EGGS
Most species are anautogenous; female requires protein to develop mature eggs
within her ovaries (about 800 µg per pair of ovaries in Phormia regina).
The females visit carrion both for protein and egg laying.
 Blow fly eggs, usually yellowish or white in color, are about 1.5 mm x 0.4 mm,
and, when laid, look like rice balls.
female blow fly typically lays 150–200 eggs per batch, she is
usually iteroparous, laying around 2,000 eggs during the course of her life.
The sex ratio of blow fly eggs is usually 50:50, but one exception is females
from two species of the genus Chrysomya (C. rufifacies and C. albiceps), which
are either arrhenogenic (laying only male offspring) or thelygenic (laying only
female offspring).
LARVAE
Hatching from an egg to larval stage takes about 8 hours to one day.
Larvae have three instars; each instar is separated by a molting event.
The larvae use proteolytic enzymes in their excreta (as well as mechanical
grinding by mouth hooks) to break down proteins on the livestock or corpse on
which they are feeding.
 Blow flies are poikilothermic – the rate at which they grow and develop is
highly dependent on temperature and species. Under room temperature (about 20
°C), the black blow fly Phormia regina can change from egg to pupa in 150–266
hours (six to 11 days).
PUPA
 When the third larval stage is complete, it will leave the corpse and burrow into
the ground to pupate, emerging as an adult seven to 14 days later.
MANAGEMENT
1. Exclusion and Sanitation
Blow Flies and Bottle Flies feed and breed on dead animals and garbage. Remove
all material where the flies can lay eggs. Killing adult flies will reduce infestation.
Proper sanitation measures must be taken with dumpsters
rodent control measures must be adaped.
Exclude Blow/Bottle Flies with proper screening and maintenance of doors and
windows.
Garbage cans and dumpsters should have tight-fitting lids and must be cleaned
regularly.
Proper drainage of water will get rid of extra moisture to avoid damp places.
Openings of buildings should be tightly screened.
2. Using Insecticides, Fly Baits and Fly Traps
Spray entry points on building or fly resting areas with insecticides such as Cyper
WP or D-Fense SC. Use these insecticides once a month.
Fly Glue Traps such as Catchmaster Gold Sticks -10.5" and Catchmaster
Goldstick - 24 " or Revenge Jumbo Fly Catchers may be used to trap and kill
Blow Flies. Goldsticks are used inside or outside. The Jumbo Fly Catchers are
perfect for hanging in gararges or basements.
Fly Bait such as Bonanza Fly Bait or Maxforce Granular Fly Bait kill flies in
60 seconds, and keep controlling them for up to 30 days.
A pyrethrum aerosol is contact insecticide which provide immediate relief. CB 80
Pyrethrin Aerosol or V One Pyrethrum Aerosol can be used.
Predators of blow flies are Spiders, Beetles, Frogs, Chickens.
FLESH FLIES (Sarcophagidae)
 The word Sarcophagidae comes from the Greek sarco=flesh, phage=eating.
commonly known as flesh flies.
 They have 108 genera and 2500 species.
 They are ovoviviparous, depositing hatched or hatching maggots instead of
lying eggs on carrion, dung, decaying material, or open wounds of mammals.
 Flesh flies can carry leprosy bacilli and can transmit intestinal
pseudomyiasis to people who eat their larvae. Flesh flies, particularly
Wohlfahrtia magnifica, can also cause myiasis in animals, mostly to sheep,
and can give them blood poisoning, or asymptomatic leprosy infections.
 Most larvae infest wounds, carrion or excrement. Larvae of some species prey
on more harmful pests, e.g Lesser house fly larvae, blow fly larvae, and
grasshopper nymphs.
SUBFAMILIES
 Sarcophaginae: Genus Sarcophaga are scavengers of small carrion, such as
dead insects and snails or smaller vertebrates. A few species feed on larger
vertebrate carcasses. Maggots occasionally eat other larvae. Both larvae and
adult eat decaying vegetable matter and excrement, and they may be found
around compost piles and pit latrines.
 Miltogramminae: They are kleptoparasites of solitary bees and solitary
wasps.
 Paramacronychiinae: This subfamily includes lepidopteran predators or
parasitoids (Agria), predators on immatures (mainly prepupae) of bumblebees
(Brachycoma) and generalist scavengers and insect predators
(Sarcophila and Wohlfahrtia).
BODY CHARACTERISTICS
 Adult is 0.08-0.9 in (4-23 mm) in length with black and gray longitudinal
stripes on thorax and checkering on the abdomen. Abdomen have bristles.
Abdominal sternites II and III are free and cover the margins of tergites.
 The posthumeral bristles are 1 or 2 in number, which is located lower than the
notopleural bristle. The hindmost posthumeral bristle is located even with or
toward the midline from the presutural bristle. Four notopleural bristles are
present and arranged in the order - short, long, short, long - from front to rear.
 The eyes are smooth, red in colour and very rarely hairy.
 The arista is plumose in its basal half, or rarely pubescent or glabrous.
LIFE CYCLE
 The life cycle for the common species can be completed in eight to 21 days. The
preferred breeding media containing decayed flesh, spoiling meat, and manure.
 They over winter as pupae in temperate climates. They emerge in spring and
mate. Eggs are laid very rarely. Eggs hatch within the body of the adult.
 Females of most species deposit 20 to 40 maggots directly in the host or
substitute. As many as 325 larvae have been known to be born by a single female.
Maggots bear 3 instars and fully developed within 3-6 days. Larvae prefer moist
and semi-aquatic environment.
 Pupae develop more quickly at cool temperatures. They are 12 mm in length and
the entire pupal stage take 16 days.
 Adult flies emerge in ten to 14 days. Several generations are produced each year.
FORENSIC IMPORTANCE
 Sarcophaga haemorrhoidalis is particularly important to forensic entomology as 
it can be one of the first in arthropods to arrive at corpse. The species is a strong 
flyer and has the ability to fly in inclement weather when other arthropod species 
are  unable  to  fly. Sarcophaga haemorrhoidalis is  most  often  associated  with 
corpses found inside buildings. 
 Like  blow  fly  species,  sarcophagid  flies  are  also  used  to  determine  the  post-
mortem  interval  (PMI)  in  death  investigations  and  because  their  development 
cycle is short, they are the most useful for the first three to four weeks after death. 
This species has also been used for PMI estimations on severely burnt remains.
 CONTROL: The burial or destruction of carcasses, manure heaps and mounds of 
decaying  organic  matter  eliminates  many  favorable  breeding  sites.  Proper 
treatment of wounds also helps to prevent flesh fly harm to animals.
Medical and Economic Importance
 20 species of Sarcophaga cause gastrointestinal myiasis and infest living tissues. 
Myiasis  occur  in  livestock  which  result  in  economic  loss.  The S.
haemorrhoidalis is  attracted  towards  animal  feces  and  prefer  to  larviposit  in 
animal sheds. Some females larviposit on mucus tissues of  rectum.
 Myiasis  occurs  from  ingesting  contaminated  food  and  infestations  may  be 
persistant if left untreated.
  Intestinal myiasis can be  difficult to diagnose as the symptoms are similar to 
other intestinal diseases and disorders, and may also be asymptomatic.
  Myiasis occurs mostly in overcrowded areas.
 Diagnosis of myiasis usually occurs when the presence of larvae in fresh stool 
samples  is  observed.  Pain  is  felt  due  to  larval  progression  down  the  intestinal 
tract  as  the  larvae  use  their  buccal  (mouth)  hooks  to  move  which  causes 
scratching along the intestinal lining. Itching and inflammation around the anal 
tissue is common in cases where larvae are deposited directly in the rectal area.
 Other forms of myiasis that can occur with S. haemorrhoidalis include nasal and 
aural infestations. Aural and nasal myiasis presents with a rancid, bloodstained 
pus, pain, and irritation in the infected tissue. Treatment consists of killing the 
maggots and removing them with forceps from the infested area.
 Adult S. haemorrhoidalis have  been  connected  with  the  transmission  of  other 
disease  organisms  including Escherichia coli (Migula), Shigella
dysenteriae (Shiga), Streptococcus spp., Salmonellaspp.,  tapeworms,  and  even 
the virus responsible for polio.

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Forensic

  • 1. FORENSIC ENTOMOLOGY  Forensic entomology involves the application of the study of biology of arthropods, including insects, arachnids, centipedes, millipedes, and crustaceans to criminal or legal cases.  Blood-feeding (haematophagous) insects For example, Mosquitoes (Culicidae). A single mosquito can be used for investigation (as a source of human DNA) even few days after taking blood.  Necrophagous insects (corpse-eating) For example, Flies, Beetles, Mites, Moths, Wasps, ants, and bees
  • 2. FLIES  Blow flies – Calliphoridae blue bottle fly, cluster fly, greenbottles, or black blowfly  Flesh flies – Sarcophagidae  House fly – Muscidae  Cheese flies – Piophilidae  Coffin flies – Phoridae  Lesser corpse flies – Sphaeroceridae  Lesser house flies – Fanniidae  Black scavenger flies – Sepsidae  Sun flies - Heleomyzidae  Black soldier fly - Stratiomyidae  Phoridae – Humpbacked flies
  • 3. BEETLES  Rove beetles – Staphylinidae  Hister beetles – Histeridae  Carrion beetles – Silphidae  Ham beetles – Cleridae  Carcass beetles – Trogidae  Skin/hide beetles – Dermestidae.  Scarab beetles – Scarabaeidae  Sap beetles – Nitidulidae    Macrocheles feed on corpses in early stage of decomposition. Tyroglyphidae and Oribatidae mites such as Rostrozetes feed on dry skin in the later stages of decomposition. MITES (Acari)
  • 4. MOTHS  Clothes-moths – (Tineidae) They are crepuscular and diurnal species. Moths feed on mammalian hair during their larval stages and may forage on any hair that remains on a body.  They are amongst the final animals contributing to the decomposition of a corpse.  Some feed on the body, some are predatory, and eat the insects feeding on the body.  Bees and wasps have been seen feeding on the corpse body during the early stages of their lifecycle. WASPS, ANTS, AND BEES
  • 5. FLIES: Calliphoridae  Blow flies, blue bottle fly, cluster fly, greenbottles, black blowfly, carrion flies, cluster flies.  About 1,100 species of blow flies are known Habitat  India, Japan, China, Central America, and the Southern United States.  The typical habitats for blow flies are temperate to tropical areas that provide a layer of loose, damp soil and litter where larvae may thrive and pupate. FOOD  Adult blow flies are occasional pollinators, being attracted to flowers with strong odors resembling rotting meat.  Larvae of most species are scavengers of carrion and dung, and most likely constitute the majority of the maggots found in such material.
  • 6. Characteristics  Adults are commonly shiny with metallic colouring, often with blue, green, or black thoraces and abdomens.  Antennae are three-segmented and aristate. The arista are plumose the entire length, and the second antennal segment is distinctly grooved. Members of Calliphoridae have branched Rs 2 veins, frontal sutures are present, and calypters are well developed.  Arrangement of hair like bristles located on the meron are used to differentiate between members of this family. Having two notopleural bristles and a hindmost posthumeral bristle located lateral to presutural bristle.  Thorax has the continuous dorsal suture across the middle, along with well- defined posterior calli. The postscutellum is absent or weakly developed. The costa is unbroken and the subcosta is apparent on the insect.
  • 7. Life cycle EGGS Most species are anautogenous; female requires protein to develop mature eggs within her ovaries (about 800 µg per pair of ovaries in Phormia regina). The females visit carrion both for protein and egg laying.  Blow fly eggs, usually yellowish or white in color, are about 1.5 mm x 0.4 mm, and, when laid, look like rice balls. female blow fly typically lays 150–200 eggs per batch, she is usually iteroparous, laying around 2,000 eggs during the course of her life. The sex ratio of blow fly eggs is usually 50:50, but one exception is females from two species of the genus Chrysomya (C. rufifacies and C. albiceps), which are either arrhenogenic (laying only male offspring) or thelygenic (laying only female offspring).
  • 8. LARVAE Hatching from an egg to larval stage takes about 8 hours to one day. Larvae have three instars; each instar is separated by a molting event. The larvae use proteolytic enzymes in their excreta (as well as mechanical grinding by mouth hooks) to break down proteins on the livestock or corpse on which they are feeding.  Blow flies are poikilothermic – the rate at which they grow and develop is highly dependent on temperature and species. Under room temperature (about 20 °C), the black blow fly Phormia regina can change from egg to pupa in 150–266 hours (six to 11 days). PUPA  When the third larval stage is complete, it will leave the corpse and burrow into the ground to pupate, emerging as an adult seven to 14 days later.
  • 9.
  • 10. MANAGEMENT 1. Exclusion and Sanitation Blow Flies and Bottle Flies feed and breed on dead animals and garbage. Remove all material where the flies can lay eggs. Killing adult flies will reduce infestation. Proper sanitation measures must be taken with dumpsters rodent control measures must be adaped. Exclude Blow/Bottle Flies with proper screening and maintenance of doors and windows. Garbage cans and dumpsters should have tight-fitting lids and must be cleaned regularly. Proper drainage of water will get rid of extra moisture to avoid damp places. Openings of buildings should be tightly screened.
  • 11. 2. Using Insecticides, Fly Baits and Fly Traps Spray entry points on building or fly resting areas with insecticides such as Cyper WP or D-Fense SC. Use these insecticides once a month. Fly Glue Traps such as Catchmaster Gold Sticks -10.5" and Catchmaster Goldstick - 24 " or Revenge Jumbo Fly Catchers may be used to trap and kill Blow Flies. Goldsticks are used inside or outside. The Jumbo Fly Catchers are perfect for hanging in gararges or basements. Fly Bait such as Bonanza Fly Bait or Maxforce Granular Fly Bait kill flies in 60 seconds, and keep controlling them for up to 30 days. A pyrethrum aerosol is contact insecticide which provide immediate relief. CB 80 Pyrethrin Aerosol or V One Pyrethrum Aerosol can be used. Predators of blow flies are Spiders, Beetles, Frogs, Chickens.
  • 12. FLESH FLIES (Sarcophagidae)  The word Sarcophagidae comes from the Greek sarco=flesh, phage=eating. commonly known as flesh flies.  They have 108 genera and 2500 species.  They are ovoviviparous, depositing hatched or hatching maggots instead of lying eggs on carrion, dung, decaying material, or open wounds of mammals.  Flesh flies can carry leprosy bacilli and can transmit intestinal pseudomyiasis to people who eat their larvae. Flesh flies, particularly Wohlfahrtia magnifica, can also cause myiasis in animals, mostly to sheep, and can give them blood poisoning, or asymptomatic leprosy infections.  Most larvae infest wounds, carrion or excrement. Larvae of some species prey on more harmful pests, e.g Lesser house fly larvae, blow fly larvae, and grasshopper nymphs.
  • 13. SUBFAMILIES  Sarcophaginae: Genus Sarcophaga are scavengers of small carrion, such as dead insects and snails or smaller vertebrates. A few species feed on larger vertebrate carcasses. Maggots occasionally eat other larvae. Both larvae and adult eat decaying vegetable matter and excrement, and they may be found around compost piles and pit latrines.  Miltogramminae: They are kleptoparasites of solitary bees and solitary wasps.  Paramacronychiinae: This subfamily includes lepidopteran predators or parasitoids (Agria), predators on immatures (mainly prepupae) of bumblebees (Brachycoma) and generalist scavengers and insect predators (Sarcophila and Wohlfahrtia).
  • 14. BODY CHARACTERISTICS  Adult is 0.08-0.9 in (4-23 mm) in length with black and gray longitudinal stripes on thorax and checkering on the abdomen. Abdomen have bristles. Abdominal sternites II and III are free and cover the margins of tergites.  The posthumeral bristles are 1 or 2 in number, which is located lower than the notopleural bristle. The hindmost posthumeral bristle is located even with or toward the midline from the presutural bristle. Four notopleural bristles are present and arranged in the order - short, long, short, long - from front to rear.  The eyes are smooth, red in colour and very rarely hairy.  The arista is plumose in its basal half, or rarely pubescent or glabrous.
  • 15. LIFE CYCLE  The life cycle for the common species can be completed in eight to 21 days. The preferred breeding media containing decayed flesh, spoiling meat, and manure.  They over winter as pupae in temperate climates. They emerge in spring and mate. Eggs are laid very rarely. Eggs hatch within the body of the adult.  Females of most species deposit 20 to 40 maggots directly in the host or substitute. As many as 325 larvae have been known to be born by a single female. Maggots bear 3 instars and fully developed within 3-6 days. Larvae prefer moist and semi-aquatic environment.  Pupae develop more quickly at cool temperatures. They are 12 mm in length and the entire pupal stage take 16 days.  Adult flies emerge in ten to 14 days. Several generations are produced each year.
  • 16.
  • 17. FORENSIC IMPORTANCE  Sarcophaga haemorrhoidalis is particularly important to forensic entomology as  it can be one of the first in arthropods to arrive at corpse. The species is a strong  flyer and has the ability to fly in inclement weather when other arthropod species  are  unable  to  fly. Sarcophaga haemorrhoidalis is  most  often  associated  with  corpses found inside buildings.   Like  blow  fly  species,  sarcophagid  flies  are  also  used  to  determine  the  post- mortem  interval  (PMI)  in  death  investigations  and  because  their  development  cycle is short, they are the most useful for the first three to four weeks after death.  This species has also been used for PMI estimations on severely burnt remains.  CONTROL: The burial or destruction of carcasses, manure heaps and mounds of  decaying  organic  matter  eliminates  many  favorable  breeding  sites.  Proper  treatment of wounds also helps to prevent flesh fly harm to animals.
  • 18. Medical and Economic Importance  20 species of Sarcophaga cause gastrointestinal myiasis and infest living tissues.  Myiasis  occur  in  livestock  which  result  in  economic  loss.  The S. haemorrhoidalis is  attracted  towards  animal  feces  and  prefer  to  larviposit  in  animal sheds. Some females larviposit on mucus tissues of  rectum.  Myiasis  occurs  from  ingesting  contaminated  food  and  infestations  may  be  persistant if left untreated.   Intestinal myiasis can be  difficult to diagnose as the symptoms are similar to  other intestinal diseases and disorders, and may also be asymptomatic.   Myiasis occurs mostly in overcrowded areas.
  • 19.  Diagnosis of myiasis usually occurs when the presence of larvae in fresh stool  samples  is  observed.  Pain  is  felt  due  to  larval  progression  down  the  intestinal  tract  as  the  larvae  use  their  buccal  (mouth)  hooks  to  move  which  causes  scratching along the intestinal lining. Itching and inflammation around the anal  tissue is common in cases where larvae are deposited directly in the rectal area.  Other forms of myiasis that can occur with S. haemorrhoidalis include nasal and  aural infestations. Aural and nasal myiasis presents with a rancid, bloodstained  pus, pain, and irritation in the infected tissue. Treatment consists of killing the  maggots and removing them with forceps from the infested area.  Adult S. haemorrhoidalis have  been  connected  with  the  transmission  of  other  disease  organisms  including Escherichia coli (Migula), Shigella dysenteriae (Shiga), Streptococcus spp., Salmonellaspp.,  tapeworms,  and  even  the virus responsible for polio.