This document summarizes a study that investigated the effect of bone marrow mesenchymal stem cells (MSCs) on wound healing in two types of diabetic mouse models. The study established full-thickness excisional wounds on STZ-induced diabetic mice and genetically diabetic BKS-DB mice. MSCs or PBS control were injected intradermally around the wounds. Wound closure was significantly faster in both diabetic mouse models that received MSCs compared to controls, demonstrating that MSCs promoted wound healing in diabetic conditions. However, MSCs had different levels of efficiency between the two mouse models, indicating differences in the wound microenvironments.

1. E-Poster: Basic Science
EP 419
EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS (MSCS) ON
DIABETIC WOUND HEALING OF DIFFERENT MICE MODELS
Biao Cheng1, Ji-ping Zou1,2, Min Xuan1 , Jian-bing Tang1,Ye-qing Deng3 , Yu Wan1
1 Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military
Command (GuangZhou, China);
2 Third Military Medical University of PLA, (Chongqing, China);
3 Guangzhou Medical University(GuangZhou, China)
Aim: To observe the promoting concrescence of diabetic wound effect of MSCs using two
kinds of experimental diabetic animal models, genetically induced spontaneous diabetes
models ( BKS-DB mice) and experimentally induced non-spontaneous diabetes models
(STZ -induced diabetic C57BL/6 mice ).
Method: To detect cell surface antigens of culture expanded MSCs with flow cytometry
and multipotential ability with differentiation experiment; To establish one of the diabetes
mouse models with STZ intraperitoneal injection and to chose the C57BLKS/Nju
spontaneous mutation diabetic mouse (BKS-DB mice) as the other; To establish two 6-mm
full-thickness excision skin wounds on back of both diabetic models; To set four groups:
STZ-PBS group vs STZ-MSCs group; BKS-PBS group vs BKS-MSCs group. To observe
wound healing when MSCs cells were injected intradermally around the wound at four
injection sites.
Results: full-thickness excisional wound model was successfully established on STZ-
induced diabetic C57BL/6 mice and BKS-DB mice; The time for heaing of STZ-MSCs
group [(12.33±0.58)d] VS STZ-PBS group[(14. 67±0.58)d p<0.05 ], BKS-BMSCs group
[(11.67±0.58)d] VS BKS-PBS group [(16.33±0.58)d p<0.05] shows that wound closure
was significantly promoted in both kinds of diabetic mice that with intradermal injection
of MSCs cells.
Conclusion: MSCs enhanced wound healing of both diabetic models, but with different
efficiency, which indicated that different wound microenvironments between the two
models exist.