Human organoid are miniature sized, self-organized structures, that are derived from stem cells or tissues in culture. The progress, potential, limitations and challenges are discussed.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
Human organoid are miniature sized, self-organized structures, that are derived from stem cells or tissues in culture. The progress, potential, limitations and challenges are discussed.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
Cell within a tumor that possess the capacity to self-renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor”.
“CSC can thus only be defined experimentally by their ability to recapitulate the generation of a continuously growing tumor”.
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.
This slide is about the potential uses of stem cells. It describes how they are useful and also puts froward the extraction process and the ares in which stem cells prove to be extremely useful. This slide also lists the various from of cells and the difference between stem cells and the normal differentiated cells. It is also richly supplied with photos and content which would altogether increase the quality of the slide. Hope you enjoy and learn. Please do like and follow. Share with your friends who might benefit from this.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ
Cell within a tumor that possess the capacity to self-renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor”.
“CSC can thus only be defined experimentally by their ability to recapitulate the generation of a continuously growing tumor”.
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.
This slide is about the potential uses of stem cells. It describes how they are useful and also puts froward the extraction process and the ares in which stem cells prove to be extremely useful. This slide also lists the various from of cells and the difference between stem cells and the normal differentiated cells. It is also richly supplied with photos and content which would altogether increase the quality of the slide. Hope you enjoy and learn. Please do like and follow. Share with your friends who might benefit from this.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Stem cells in regenerative biology and medicinePasteur_Tunis
Présentation réalisée par Shahragim Tajbakhsh durant le cours du réseau international des instituts Pasteur de "Médecine Génomique: du diagnostic à la thérapie " (17-21 octobre 2016)
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ
Embryonic stem cells – Promises and IssuesTania Jabin
Introduction, Embryonic Stem Cells, Promises of Embryonic Stem cell research, Figure: The Promise of Stem Cell Research, Issues in Embryonic Stem cells - New embryonic stem cell lines from frozen embryos Informed consent for donation of materials for stem cell research Waiver of consent Consent from gamete donors Confidentiality of donor information Ethical concerns about oocyte donation for research (1. Medical risks of oocyte retrieval, 2. Protecting the reproductive interests of women in infertility treatment, 3. Payment to oocyte donors, 4. Informed consent for oocyte donation).
Introduction.
Properties of Stem Cells.
Key Research events.
Embryonic Stem Cell.
Stem cell Cultivation.
Stem cells are central to three processes in an organism.
Research & Clinical Application of stem cell.
Research patents.
Conclusion.
Reference.
A Brief History of Regenerative MedicineJohn Makohen
In the presentation ISREGEN outlines the history of regenerative medicine fro it's earliest days when Robert Briggs and Thomas King began cloning frogs to the present medicinal advancements in stem cell research and repair.
1. Organoids: Small Cells Doing Big Things
Franklin T Wilson
Introduction
Organoids are small three-
dimensional tissues that mimic the
function of it’s larger organ origin.
They were first propagated in 2007
by Hans Clever with a “mini-gut”, and
have since then exploded in
popularity among researchers. Today,
there are many different types of
organoids including cerebral,
pancreatic, and cardiac cells. The
possible uses for these external mini-
organs are obviously far-reaching,
but the time and cost to produce
them is still relatively high. However,
with the constant technological
advancements being made by
researchers everyday, the time and
cost for cultivating these organoids is
decreasing everyday.
The goal of this research was to
- Learn the process of cultivating
organoids
- Learn about the different organoid
tissues being grown presently
- Explore the potential of organoids in
the medical, pharmaceutical, and
biotechnological fields
Chi, K. R. (2015, September 1). Orchestrating Organoids. In The Scientist.
Mahe, M. M., Sundaram, N., Watson, C. L., Shroyer, N. F., Helmrath, M. A.
Establishment of Human Epithelial Enteroids and Colonoids from
Whole Tissue and Biopsy. J. Vis. Exp. (97), e52483, doi:10.3791/52483
(2015).
Nantasanti, S., De Bruin, A., Rothuizen, J., Penning, L. C., & Schotanus, B.
A. (2016, January 21). Concise Review: Organoids Are a Powerful Tool
for the Study of Liver Disease and Personalized Treatment Design in
Humans and Animals [Electronic version]. Stem Cells Translation
Medicine, 5(3), 325-330. doi:10.5966
Ramachandran SD, Schirmer K, Münst B, Heinz S, Ghafoory S, Wölfl S, et
al. (2015) In Vitro Generation of Functional Liver Organoid-Like
Structures Using Adult Human Cells. PLoS ONE 10(10): e0139345.
doi:10.1371/journal.pone.0139345
Richardson, K. (2013, September 10). Wake Forest Baptist Leads $24 million
Project to Develop “Body on a Chip”. In Wake Forest Babtist Medical
Center. Retrieved April 6, 2016.
Willyard, C. (2015, July 17). The Boom in Mini Stomachs, Brains, Breasts,
Kidneys and More. In Nature.
Although organoids are still in their
relative infancy as a field of research,
they are already showing a great
amount of potential. As our
methodologies and techniques
continue to advance for cultivating
them, so does our potential for
medical and scientific use and
understanding.
The basic premise of cultivating
organoids is to harvest pluripotent stem
cells (PSCs) from a desired organ. The
cells are then cultured the cells in a
nutritional gel-like environment, allowing
them to grow in three dimensions.
Through the use of certain stimulators
and procedures, they are able to
“convince” the cells to grow, and they
naturally form themselves into a ball-like
structure. This structure, although not
complete by any means, has the basic
elements of a miniature organ without
the need for a blood supply, as it is
nourished by the surrounding gel.
The method of research was largely
online scholarly article-based study.
Thanks to Dr. Barlow at GTCC, peer-
reviewed journal articles were easily
obtained that otherwise would have
been inaccessible for this research.
Results
Organoid derivation and development (credit: Rene Anand and Susan McKay)
As the list of organoid types grows, so
does the potential uses for them. At
Wake Forest Institute of Regenerative
Medicine for example, they are already
able to produce cardiac and liver
organoids that can be used to test
harmful “chemical and biological
agents” on tissues without having to
use live animals, which is beneficial for
a multitude of reasons. From there,
they will be potentially able to produce
anecdotes and treatments for these
biological agents, all potentially without
the use of laboratory animals.