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International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016]
Page | 35
Evaluation of various Synthetic Insecticides against Thrips
(Thrips tabaci) in Bt Cotton
Rita sharma1
, Leena sharan2
1,2
Laboratory of environmental Biology, P.G. Department of Zoology, Govt. Dungar P.G.college, Bikaner
Email address- sharmarita333@gmail.com
Abstract— Cotton (Gossypium spp.) popularly known as ‘white gold’ is one of the most ancient important cash crop of
India. Apart from its value as fibre, it has great potential to be used as edible oil, food for animals and other by products like
particle board and boxes. The development of Bt cotton containing a genetically introgresses endotoxin gene from the gram
negative soil bacteria (Bacillus thuringiensis Hubner) represents a significant technological land mark in the global cotton
research. Thrips is a major sucking pest on cotton crop, causing quantitative and qualitative losses to cotton. An experiment
was conducted at Agriculture Research Station, Sri Ganganagar in Randomized Block Design with 10 treatments including
control and replicated three times, to evaluate various insecticides against thrips in Bt cotton. The study revealed that
maximum reduction (56.00%) in thrips population was recorded with the treatment of Acephate 75% SP, followed by
Imidacloprid 17.8% SL (49.66%). Both the treatments were at par and significantly superior over the other treatments. The
phytotoxic effect on crop could not be observed during the experimental period.
Keywords— Cotton, Agriculture Research, Acephate, soil bacteria, Acephate.
I. INTRODUCTION
Cotton is one of the prominent industrial and economic crops of india. The pest spectrum of cotton crop is quite complex and
these pests not only reduce the yield but also adversely affect the quality of lint and seed. The development of Bt cotton
containing a genetically introgresses endotoxin gene from the gram negative soil bacteria (Bacillus thuringiensis Hubner)
represents a significant technological land mark in the global cotton research. India adopted this technology in 2002-03. Last
few decades bollworm attack on cotton was a serious problem but, with the introduction of Bt varieties of cotton this problem
has been solved to some extent and a significant change in cropping scheme in the cotton growing areas has been observed
(Ahsan & Altaf, 2009). But the problem of sucking insect pests attack is remained unsolved still now. Among them thrips is
most destructive sucking pest. Thrips attack cotton crop early in the season, but high population densities can be seen during
second fortnight in September (Gupta et al., 1997, Khan et al., 2008). Both nymph and adult stages of thrips damage the
tissue and destroy leaves by sucking cell sap. Due to the attack of this pest, leaves curl up and in the case of several attack,
plant remains stunted at initial stage.This insect is widely distributed in northern area. The chemical control is the one of the
rapid methods to reduce the losses caused by sucking insect pests to the cotton crop (Gogi et al., 2006). Many new chemical
insecticides are introduced in the market for management thrips therefore, the present study was undertaken to evaluate the
various insecticides for management of thrips (Thrips tabaci) in Bt cotton.
II. MATERIALS AND METHODS
The experiment was conducted in Randomized Block design with 10 treatments including Control and three replication at the
Agriculture Research Station, Sri Ganganagar (Raj.). The crop was raised in 10 m x 5 m plotes with 100 cm row to row and
60 cm plant to plant distance. Total four sprays of each insecticide viz., Acephate 75% SP, Thiomethoxam 25% WG,
Acetamiprid 20% SP, Diafenthiuron 50% SP, Calypso 24% OD, Sulfoxaflor 24% SC, Triazophos 40% EC, Imidacloprid
17.8% SL and Pyriproxyfen 10% EC were given at 10-15 days interval depending upon climatic condition. The efficacy of
various insecticides will be assessed by counting the living population of thrips on 5 randomly selected plants in each plot 24
hours before and after 1, 3, 5 and 10 days of treatment. The observations on the surviving insects after each treatment at
definate time intervals were recorded and percent reduction in population was calculated. Percent reduction was calculated
by using the below mentioned formula:
International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016]
Page | 36
The data recorded were presented as mean values, which were subjected to Analysis of Variance (ANOVA) and Critical
Difference (CD) was worked out to compare different treatments. For Analysis of Variance, the data on percentage reduction
were transformed into angular values. The critical difference calculated was compared between mean percent reductions at
definite time interval.
III. RESULTS AND DISCUSSION
The evaluation of various insecticides against thrip population depicted in the Table-1 clearly indicates that thrip population
was significantly low in all the plots treated with insecticides over control. The study revealed that maximum reduction
(56.00%) in thrips population was recorded with the treatment of Acephate 75% SP, followed by Imidacloprid 17.8% SL
(49.66%). Both the treatments were at par and significantly superior over the other treatments. The phytotoxic effect on crop
could not be observed during the experimental period. . In our experiment, nine chemicals were tested against thrips under
field conditions, among these chemicals some were found effective against thrips while others were found to be less
effective. Our results suggested that, Acephate 75% SP and Imidacloprid 17.8% SL were proved to be the most effective
insecticide resulted in significant reduction of thrip populations as compared to all other insecticides . The results agree with
of the findings of Iqbal et al., (2013) who reported maximum control of thrips population with the treatment of Acephate.
Similar trend was observed by Pachundkar et al., (2013) and Patil et al., (2014) who concluded that Acephate was found to
be highly effective against thrips population. The present results also get support from the observations of Kolhe et al.,
(2009) who found that Imidacloprid was most effective against thrips. The treatment module in the applied does not show
any phytotoxic effect on cotton plants.
TABLE 1
BIO-EFFICACY OF VARIOUS INSECTICIDES ON PERCENT REDUCTION OF THRIPS IN BT COTTON
Treatment Doses (a. i./ha)
Pooled mean of four sprays
Mean % Reduction of thrips / 3 leaves
Pooled
1 DAS 3 DAS 5 DAS 10 DAS
Acephate 75% SP 800 g 62.22# (51.09)*
58.51#
(45.38)*
52.51#
(46.42)*
50.75#
(45.41)*
56.00#
(47.08)*
Thiamethoxam 25% WG 200 g 42.40 (40.94)
35.46
(40.94)
34.78
(36.03)
28.25
(31.90)
35.22
(37.45)
Acetamiprid 20% SP 160 g 49.02 (43.20)
42.69
(40.62)
38.15
(38.09)
29.02
(32.54)
39.72
(38.61)
Diafenthiuron 50% SP 800 g 35.58 (38.85)
33.66
(33.00)
28.81
(32.32)
14.96
(22.56)
28.25
(31.68)
Calypso 24% OD 400 g 23.44 (28.15)
21.85
(26.17)
20.61
(26.82)
19.89
(26.37)
21.44
(26.88)
Sulfoxaflor 24% SC 400 g 34.47 (33.79)
30.44
(32.71)
26.12
(30.63)
23.13
(28.63)
28.54
(31.44)
Triazophos 40 % EC 1000 ml 40.40 (43.29)
32.87
(38.46)
30.87
(33.68)
24.72
(29.18)
32.22
(36.15)
Imidacloprid 17.8% SL 133 ml 59.26 (51.01)
55.83
(46.42)
46.38
(42.88)
37.19
(37.51)
49.66
(44.46)
Pyriproxyfen 10 % EC 400 ml 27.79 (32.44)
24.73
(30.75)
21.46
(27.44)
18.67
(25.36)
23.16
(29.00)
Control 1.98 (7.86)
2.39
(8.14)
2.63
(9.19)
2.41
(8.80)
2.35
(8.50)
SEM 1.88 1.65 1.75 1.50 1.47
CD 5.58 4.89 5.19 4.45 4.37
CV% 7.69 7.76 8.78 8.03 8.91
# Pooled mean of four spyays
*Values in parenthesis are transformed angular values
DAS Days after spray
International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016]
Page | 37
FIGURE 1: MEAN % REDUCTION OF THRIPS / 3 LEAVES
IV. CONCLUSION
Thrip population was significantly low in all the plots treated with insecticides over control. Our results suggested that,
Acephate 75% SP and Imidacloprid 17.8% SL were proved to be the most effective insecticide resulted in significant
reduction of thrip populations as compared to all other insecticides . Both the treatments were at par and significantly
superior over the other treatments. The phytotoxic effect on crop could not be observed during the experimental period.
REFERENCES
[1] Ahsan, R. and Altaf, Z. (2009). Development adoption and performance of Bt cotton in Pakistan: A review. Pak. J. Agri. Sci., 22: 73-
85.
[2] Gogi, M. D., Sarfraj, R. M., Dosdall, L. M., Arif, M. J., Keddie, A. B. and Ashfaq M. (2006). Effectiveness of two insect growth
regulators against Bemisia tabaci and Helicoverpa armigera and their impact on population densities of arthropod predators in cotton
in Pakistan. Pest management Sci., 62: 982-990.
[3] Gupta, M P, Sharma, S. and Shrivastava, S. K. (1997). Population buildup of some sap-sucking insect on cotton in M.P. J. insect sci.,
10 : 53-56.
[4] Iqbal, J., Nadeem, M., Assi, M. S., Fiaz, M. M. and Hassan, M. W. U. (2013). Comparative efficacy of some insecticides against
sucking insect pests on Mungbean, Vigna Radiata (L.) Wilczek.Gomal Uni. J. Res., 29 (1) : 31-37.
[5] Khan, M.A., Khaliq, A., Subhani, M.N. and Saleem, M. W.(2008). Incidence and development of Thrips tabaci and Tetranychus
urticae on field grown cotton. Int. J. Agric. Biol., 10 : 232-234.
[6] Kolhe, A. V., Nawod, S. S., Patil, B. R. and Ingole, O. V. (2009). Bioefficacy of newer insecticides against sucking Pests of cotton. J.
Cotton Res. Dev., 23 (1) : 146-148.
[7] Pachundkar, N. N., Borad, P. K. and Patil, P. A. (2013). Evaluation of various synthetic insecticides against sucking insect pests of
cluster Bean. IJSRP., 3 (8) : 1-6.
[8] Patil, S. B., Udikari, S. S. and Badiger, H. K. (2014). New Formulation of acephate- A Promising Alternative for sucking pest
management in Bt Cotton. Pestology, XXXVIII (7) : 39-42.

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Evaluation of various Synthetic Insecticides against Thrips (Thrips tabaci) in Bt Cotton

  • 1. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016] Page | 35 Evaluation of various Synthetic Insecticides against Thrips (Thrips tabaci) in Bt Cotton Rita sharma1 , Leena sharan2 1,2 Laboratory of environmental Biology, P.G. Department of Zoology, Govt. Dungar P.G.college, Bikaner Email address- sharmarita333@gmail.com Abstract— Cotton (Gossypium spp.) popularly known as ‘white gold’ is one of the most ancient important cash crop of India. Apart from its value as fibre, it has great potential to be used as edible oil, food for animals and other by products like particle board and boxes. The development of Bt cotton containing a genetically introgresses endotoxin gene from the gram negative soil bacteria (Bacillus thuringiensis Hubner) represents a significant technological land mark in the global cotton research. Thrips is a major sucking pest on cotton crop, causing quantitative and qualitative losses to cotton. An experiment was conducted at Agriculture Research Station, Sri Ganganagar in Randomized Block Design with 10 treatments including control and replicated three times, to evaluate various insecticides against thrips in Bt cotton. The study revealed that maximum reduction (56.00%) in thrips population was recorded with the treatment of Acephate 75% SP, followed by Imidacloprid 17.8% SL (49.66%). Both the treatments were at par and significantly superior over the other treatments. The phytotoxic effect on crop could not be observed during the experimental period. Keywords— Cotton, Agriculture Research, Acephate, soil bacteria, Acephate. I. INTRODUCTION Cotton is one of the prominent industrial and economic crops of india. The pest spectrum of cotton crop is quite complex and these pests not only reduce the yield but also adversely affect the quality of lint and seed. The development of Bt cotton containing a genetically introgresses endotoxin gene from the gram negative soil bacteria (Bacillus thuringiensis Hubner) represents a significant technological land mark in the global cotton research. India adopted this technology in 2002-03. Last few decades bollworm attack on cotton was a serious problem but, with the introduction of Bt varieties of cotton this problem has been solved to some extent and a significant change in cropping scheme in the cotton growing areas has been observed (Ahsan & Altaf, 2009). But the problem of sucking insect pests attack is remained unsolved still now. Among them thrips is most destructive sucking pest. Thrips attack cotton crop early in the season, but high population densities can be seen during second fortnight in September (Gupta et al., 1997, Khan et al., 2008). Both nymph and adult stages of thrips damage the tissue and destroy leaves by sucking cell sap. Due to the attack of this pest, leaves curl up and in the case of several attack, plant remains stunted at initial stage.This insect is widely distributed in northern area. The chemical control is the one of the rapid methods to reduce the losses caused by sucking insect pests to the cotton crop (Gogi et al., 2006). Many new chemical insecticides are introduced in the market for management thrips therefore, the present study was undertaken to evaluate the various insecticides for management of thrips (Thrips tabaci) in Bt cotton. II. MATERIALS AND METHODS The experiment was conducted in Randomized Block design with 10 treatments including Control and three replication at the Agriculture Research Station, Sri Ganganagar (Raj.). The crop was raised in 10 m x 5 m plotes with 100 cm row to row and 60 cm plant to plant distance. Total four sprays of each insecticide viz., Acephate 75% SP, Thiomethoxam 25% WG, Acetamiprid 20% SP, Diafenthiuron 50% SP, Calypso 24% OD, Sulfoxaflor 24% SC, Triazophos 40% EC, Imidacloprid 17.8% SL and Pyriproxyfen 10% EC were given at 10-15 days interval depending upon climatic condition. The efficacy of various insecticides will be assessed by counting the living population of thrips on 5 randomly selected plants in each plot 24 hours before and after 1, 3, 5 and 10 days of treatment. The observations on the surviving insects after each treatment at definate time intervals were recorded and percent reduction in population was calculated. Percent reduction was calculated by using the below mentioned formula:
  • 2. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016] Page | 36 The data recorded were presented as mean values, which were subjected to Analysis of Variance (ANOVA) and Critical Difference (CD) was worked out to compare different treatments. For Analysis of Variance, the data on percentage reduction were transformed into angular values. The critical difference calculated was compared between mean percent reductions at definite time interval. III. RESULTS AND DISCUSSION The evaluation of various insecticides against thrip population depicted in the Table-1 clearly indicates that thrip population was significantly low in all the plots treated with insecticides over control. The study revealed that maximum reduction (56.00%) in thrips population was recorded with the treatment of Acephate 75% SP, followed by Imidacloprid 17.8% SL (49.66%). Both the treatments were at par and significantly superior over the other treatments. The phytotoxic effect on crop could not be observed during the experimental period. . In our experiment, nine chemicals were tested against thrips under field conditions, among these chemicals some were found effective against thrips while others were found to be less effective. Our results suggested that, Acephate 75% SP and Imidacloprid 17.8% SL were proved to be the most effective insecticide resulted in significant reduction of thrip populations as compared to all other insecticides . The results agree with of the findings of Iqbal et al., (2013) who reported maximum control of thrips population with the treatment of Acephate. Similar trend was observed by Pachundkar et al., (2013) and Patil et al., (2014) who concluded that Acephate was found to be highly effective against thrips population. The present results also get support from the observations of Kolhe et al., (2009) who found that Imidacloprid was most effective against thrips. The treatment module in the applied does not show any phytotoxic effect on cotton plants. TABLE 1 BIO-EFFICACY OF VARIOUS INSECTICIDES ON PERCENT REDUCTION OF THRIPS IN BT COTTON Treatment Doses (a. i./ha) Pooled mean of four sprays Mean % Reduction of thrips / 3 leaves Pooled 1 DAS 3 DAS 5 DAS 10 DAS Acephate 75% SP 800 g 62.22# (51.09)* 58.51# (45.38)* 52.51# (46.42)* 50.75# (45.41)* 56.00# (47.08)* Thiamethoxam 25% WG 200 g 42.40 (40.94) 35.46 (40.94) 34.78 (36.03) 28.25 (31.90) 35.22 (37.45) Acetamiprid 20% SP 160 g 49.02 (43.20) 42.69 (40.62) 38.15 (38.09) 29.02 (32.54) 39.72 (38.61) Diafenthiuron 50% SP 800 g 35.58 (38.85) 33.66 (33.00) 28.81 (32.32) 14.96 (22.56) 28.25 (31.68) Calypso 24% OD 400 g 23.44 (28.15) 21.85 (26.17) 20.61 (26.82) 19.89 (26.37) 21.44 (26.88) Sulfoxaflor 24% SC 400 g 34.47 (33.79) 30.44 (32.71) 26.12 (30.63) 23.13 (28.63) 28.54 (31.44) Triazophos 40 % EC 1000 ml 40.40 (43.29) 32.87 (38.46) 30.87 (33.68) 24.72 (29.18) 32.22 (36.15) Imidacloprid 17.8% SL 133 ml 59.26 (51.01) 55.83 (46.42) 46.38 (42.88) 37.19 (37.51) 49.66 (44.46) Pyriproxyfen 10 % EC 400 ml 27.79 (32.44) 24.73 (30.75) 21.46 (27.44) 18.67 (25.36) 23.16 (29.00) Control 1.98 (7.86) 2.39 (8.14) 2.63 (9.19) 2.41 (8.80) 2.35 (8.50) SEM 1.88 1.65 1.75 1.50 1.47 CD 5.58 4.89 5.19 4.45 4.37 CV% 7.69 7.76 8.78 8.03 8.91 # Pooled mean of four spyays *Values in parenthesis are transformed angular values DAS Days after spray
  • 3. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-8, August- 2016] Page | 37 FIGURE 1: MEAN % REDUCTION OF THRIPS / 3 LEAVES IV. CONCLUSION Thrip population was significantly low in all the plots treated with insecticides over control. Our results suggested that, Acephate 75% SP and Imidacloprid 17.8% SL were proved to be the most effective insecticide resulted in significant reduction of thrip populations as compared to all other insecticides . Both the treatments were at par and significantly superior over the other treatments. The phytotoxic effect on crop could not be observed during the experimental period. REFERENCES [1] Ahsan, R. and Altaf, Z. (2009). Development adoption and performance of Bt cotton in Pakistan: A review. Pak. J. Agri. Sci., 22: 73- 85. [2] Gogi, M. D., Sarfraj, R. M., Dosdall, L. M., Arif, M. J., Keddie, A. B. and Ashfaq M. (2006). Effectiveness of two insect growth regulators against Bemisia tabaci and Helicoverpa armigera and their impact on population densities of arthropod predators in cotton in Pakistan. Pest management Sci., 62: 982-990. [3] Gupta, M P, Sharma, S. and Shrivastava, S. K. (1997). Population buildup of some sap-sucking insect on cotton in M.P. J. insect sci., 10 : 53-56. [4] Iqbal, J., Nadeem, M., Assi, M. S., Fiaz, M. M. and Hassan, M. W. U. (2013). Comparative efficacy of some insecticides against sucking insect pests on Mungbean, Vigna Radiata (L.) Wilczek.Gomal Uni. J. Res., 29 (1) : 31-37. [5] Khan, M.A., Khaliq, A., Subhani, M.N. and Saleem, M. W.(2008). Incidence and development of Thrips tabaci and Tetranychus urticae on field grown cotton. Int. J. Agric. Biol., 10 : 232-234. [6] Kolhe, A. V., Nawod, S. S., Patil, B. R. and Ingole, O. V. (2009). Bioefficacy of newer insecticides against sucking Pests of cotton. J. Cotton Res. Dev., 23 (1) : 146-148. [7] Pachundkar, N. N., Borad, P. K. and Patil, P. A. (2013). Evaluation of various synthetic insecticides against sucking insect pests of cluster Bean. IJSRP., 3 (8) : 1-6. [8] Patil, S. B., Udikari, S. S. and Badiger, H. K. (2014). New Formulation of acephate- A Promising Alternative for sucking pest management in Bt Cotton. Pestology, XXXVIII (7) : 39-42.