Erythropoiesis is the process of red blood cell formation that occurs in bone marrow. It involves stem cells maturing through several stages over 7-9 days to become reticulocytes and then erythrocytes. The cytoplasm changes color as maturation occurs and cell size decreases as the nucleus is lost. Erythropoietin produced by kidneys is the major hormonal regulator of erythropoiesis, stimulating stem cell development and red blood cell maturation. A variety of nutritional and environmental factors can also influence erythropoiesis.
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Blood is a body fluid in humans and other animals that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells. In vertebrates, it is composed of blood cells suspended in blood plasma.
Factors responsible for erythropoiesis. Development and maturation of erythrocytes require mostly three types of factors
1. General factors 2. Maturation factors 3. Factors necessary for hemoglobin formation.
Blood is a body fluid in humans and other animals that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells. In vertebrates, it is composed of blood cells suspended in blood plasma.
Factors responsible for erythropoiesis. Development and maturation of erythrocytes require mostly three types of factors
1. General factors 2. Maturation factors 3. Factors necessary for hemoglobin formation.
ERYTHROCYTES
- Major function - carry O2 , CO2, buffer
- Contain Haemoglobin (Fe atoms)
- 14 gms/100ml
- Biconcave disc
- High surface to volume ratio
Plasma membrane contain special polysaccharide & proteins - spectrin
- Differ from person to person - blood type/group
Normal count 4.5 - 5 million/cumm
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Blood is considered a connective tissue because it has a matrix. ... Blood Tissue: Blood is a connective tissue that has a fluid matrix, called plasma, and no fibers. Erythrocytes (red blood cells), the predominant cell type, are involved in the transport of oxygen and carbon dioxide.
Apoptosis also known as cell suicide. Difference between necrosis and apoptosis. Changes in apoptosis. Mechanism of apoptosis. Functional significance of apoptosis. Applied aspects of apoptosis
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
2. OBJECTIVES
To get a better understanding of :
Definition of erythropoiesis
Stages of erythropoiesis
Description of various cell stages
Duration of erythropoiesis
Factors affecting erythropoiesis
Description of erythropoietin
3. INTRODUCTION
The process of formation of Red Blood Cells
(RBCs) is called Erythropoiesis.
It is a part of the process of development of
blood cells called Hematopoiesis which
include:
Erythropoiesis
Leucopoiesis – formation of White Blood
Cells
Thrombopoiesis – formation of platelets
4. STAGES OF ERYTHROPOIESIS
There are three stages of erythropoiesis:
Mesoblastic stage
Medullary stage and
Hepatic stage
MESOBLASTIC STAGE
During intrauterine life, erythropoieisis first
takes place in the mesoderm of yolk sac &
mesoderm of the body. This is mesoblastic stage.
During this stage, erythropoiesis is
intravascular.
This takes place in the first 2 months of
gestation.
5. CONTD..
HEPATIC STAGE
From the 5th week of gestation, erythropoiesis
takes place in the liver & spleen. This is the
hepatic stage.
MEDULLARY STAGE
From the 5th month of gestation, erythropoiesis
takes place in the bone marrow – medullary
stage.
It is usually slow in the 2nd trimester and
becomes more effective in the third trimester.
6.
7. CONTD..
After birth bone marrow becomes the sole site
of erythropoiesis.
In young children, active hematopoietic bone
marrow is found in both axial skeleton and
bones of extremities.
The active hematopoietic bone marrow is red
in color due to marked cellularity – Red bone
marrow.
However, there occurs a progressive fatty
replacement throughout the long bones
converting red bone marrow to the yellow
bone marrow.
8. CONTD..
After 20-30 years, erythropoiesis is mostly
limited to sternum, ribs, vertebrae, skull, pelvic
and pectoral girdles.
9.
10. STEPS OF ERYTHROPOIESIS
There are four major cell stages
Stem cells
Progenitor cells
Precursor cells and
Mature cells.
During erythropoiesis, following cellular changes takes
place :
Cell size progressively decreases.
Size of nucleus and no. of nucleoli decreases.
Chromatin material condenses and nucleus
disappears.
Staining reaction of cytoplasm – deep basophilic to
acidophilic
11. STEM CELLS
Pluripotent stem cells are the mother stem
cells that form stem cells for different cell
lines.
It possess two fundamental properties :
Self replication – they are capable of
giving rise to more stem cells
Differentiation – they have the ability to
differentiate into specialized cells called
progenitor cells.
12.
13. COMMITED STEM CELLS
These cells develop from pluripotent stem
cells.
They are of two categories :
Myeloid stem cell and
Lymphoid stem cell
Myeloid stem cells form erythroid series,
monocytic granulocytic series and
megakaryoid series.
Erythroid stem cells give rise to progenitor
cells for erythroid cell lines.
14.
15. PROGENITOR CELLS
Have the ability to give rise to clones.
They are of two types:
BFU – E (Burst Forming Units)
CFU – E (Colony Forming Units)
BFU – E & CFU – E develop from CFU –
Mg/E (Megakaryoid/Erythroid)
BFU – E give rise to large number of CFU –
Erythroid cells.
CFU – E give rise to Blast cells.
16. PRECURSOR CELLS/BLAST CELLS
The precursors for erythrocytes –
erythroblasts or normoblasts.
Normoblasts develop from pronormoblasts.
Normoblasts have three successive forms :
Early normoblasts
Intermediate normoblasts and
Late normoblasts.
17. PRONORMOBLAST
First blast cells to appear in the Bone Marrow and
the first identifiable cells of erythroid series.
MORPHOLOGICAL FEATURES
1. Size : 15 – 20 µm.
2. Shape : irregularly rounded or slightly oval
3. Cytoplasm : Less, Basophilic due to the
presence of polyribosomes, high content of
RNA.
4. Nucleus : Large, multiple nucleoli.
5. Mitosis +
6. Hb : not yet formed.
18.
19. EARLY/ BASOPHILIC NORMOBLAST
Pronormoblast progresses into early
normoblast.
MORPHOLOGICAL FEATURES
1. Size : 12–16µm.
2. Shape : irregularly rounded or slightly oval
3. Cytoplasm : Scanty, Blue, Basophilic.
4. Nucleus : Large, Chromatin strands are
thicker.
5. Mitosis +
6. Hb : not present.
20.
21. INTERMEDIATE NORMOBLAST
This appears following mitotic division of
early normoblast.
Also called as polychromatophilic
normoblast.
MORPHOLOGICAL FEATURES
1. Size : 10 – 14 µm.
2. Cytoplasm : polychromatophilic (contains
admixture of basophilic RNA and
acidophilic Hb )
22. 3. Nucleus : Coarse, condense, deeply
basophilic with no nucleoli.
4. Mitosis +
5. Hb : appears.
23. LATE NORMOBLAST
Last nucleated cell of erythrocyte series.
Also called as orthochromatophilic normoblast.
MORPHOLOGICAL FEATURES
1. Size : 8 – 10 µm.
2. Cytoplasm : Deeply acidophilic with diffuse
basophilic hue. It gives an appearance of an
orthochromatic cell.
3. Nucleus : Small, pyknotic with dark chromatin.
4. Mitosis : absent.
5. Hb +
24.
25. RETICULOCYTES
Immediate precursors of red cells.
Also called as juvenile red cells.
MORPHOLOGICAL FEATURES
1. Size : 7 – 7.5 µm.
2. Cytoplasm : Contains small amounts of RNA. With
supravital stains like brilliant cresyl blue, the RNA
appears in the form of reticulum and hence the cell is
called reticulocyte.
3. Nucleus : absent
4. Mitosis : absent.
5. Hb : increases.
26.
27. ERYTHROCYTES
Final cells in erythropoiesis.
Reticulocytes spends 1 – 2 days in the marrow
and circulate for 1 – 2 days in the peripheral
blood before maturing to form erythrocytes.
MORPHOLOGICAL FEATURES
1. Size : 7.5 µm.
2. Shape : Biconcave disc
3. No nucleus
4. Hb : +
28.
29.
30. FATE OF ERYTHROCYTES
Ageing erythrocytes are destroyed, often in
the spleen, after an average life span of 120
days.
The phagocytic cells of reticulo endothelial
system degrade the haemoglobin released,
with iron from the haem and amino acids
from the globulin molecules being recycled.
The porphyrin ring is converted into
bilurubin, which is further metabolized by
the liver and then excreted in the bile.
32. DURATION
The total period of erythropoiesis is 7 – 9
days.
It takes 5 – 7 days for progenitor cells to
become reticulocytes and another 2 days
for reticulocytes to become red cells.
33. FACTORS CONTROLLING ERYTHROPOIESIS
Hormonal
Erythropoietin (EPO)
Interleukins and GM – CSF
Androgen and estrogen
Dietary
Iron
Vit B12 and folic acid
Proteins
Vitamin C
Copper, cobalt
Other factors
Environmental
Drugs and chemicals
34. HORMONAL FACTORS
ERYTHROPOIETIN
In 1906, Carnot and Deflandre proposed an alternate
mechanism for hypoxic induction of erythropoiesis.
They observed an increase in red blood cell counts
following infusion of normal rabbits with serum from
anemic animals and concluded that erythropoiesis is
regulated by a humoral “factor” in the plasma which
they called hemopoietin.
in 1948 two Finnish scientists, Bonsdorff and Jalavisto
continued work on red blood cell production and
called the hemopoietic substance "erythropoietin“.
In 1957, Jacobson and co-workers found that EPO is
produced by kidneys.
35. ERYTHROPOIETIN (EPO)
Also known as hematopoietin.
SOURCE
Produced mainly by the interstitial cells in
peritubular capillary bed of kidneys.
Also produced by juxtaglomerular cells and
extraglomerular mesangial cells .
Kidney – 85% of EPO secretion; Kuppfer cells and
prevenous hepatocytes in Liver – 15% of EPO
production.
36. EPO
STRUCTURE
EPO is a glycoprotein containing 165 amino acids.
Molecular weight – 34,000 Dalton.
STIMULUS FOR PRODUCTION
RBC function – supply O2 to the tissues.
Whenever there’s hypoxia / decrease in the no. of
RBCs, there occurs a release of renal erythropoietin
factor.
If the no. of RBCs is more, EPO formation is less.
37. EPO
MECHANISM OF ACTION
Hypoxia → ↑ release of Hypoxia Inducible Factor
(HIF -1)
HIF – 1 binds to Hypoxia Response Element (HRE)
of EPO gene that ↑ synthesis of EPO.
EPO binds to receptors in the target cells.
The receptors have tyrosine kinase activity and
activates a cascade of serine and threonine kinases
resulting in the growth & development of target
cells.
Thus, EPO ↑ committed stem cells which mature to
erythrocytes.
38.
39. APPLIED ASPECT
As kidney is the main source of EPO,
chronic renal diseases that reduce renal
mass produce anemia.
Anemia is also caused following
nephrectomy since the amount
produced by Liver doesn’t meet the
needs.
40. EPO
FUNCTIONS
Stimulates BFU – E & CFU – E to form pro
normoblasts.
Enhances mitosis.
Facilitates maturation of normoblasts.
Increases Hb synthesis in normoblasts.
Acts on stem cells to promote their transformation
towards erythroid series.
Stimulates early release of reticulocytes in
circulation.
41. EPO
REGULATION
Factors that ↑ EPO
Hypoxia, Anemia, ↓ Blood volume, Lung diseases.
Hormones like epinephrine, nor epinephrine,
androgen, thyroxine, prolactin.
Factors that ↓ EPO
Estrogen, theophylline.
METABOLISM
Inactivated mainly in the liver.
Half life in circulation is about 5 hrs.
42. HORMONAL FACTORS
INTERLEUKINS AND GM – CSF
IL – 1, 3 and 5 produced from T- cells act on stem
cells and convert them to progenitor cells.
GM – CSF stimulates the production of committed
stem cells.
ANDROGEN AND ESTROGEN
Androgen stimulate erythropoiesis. Hence males
have ↑ RBCs
Estrogen inhibit erythropoiesis by ↓ EPO
production and ↓ hepatic synthesis of globulin.
43. DIETARY FACTORS
IRON
Iron is the raw material for synthesis of heme
component of hemoglobin.
Iron deficiency results in microcytic hypochromic
anemia.
VIT B12 & FOLIC ACID
Necessary for the maturation of red cells as they
promote DNA synthesis.
For synthesis of DNA, thymine is required &
Tetrahydrofolate (THF) is necessary for thymine
synthesis which is formed from folic acid.
44. DIETARY FACTORS
Folate deficiency leads to arrest of mitosis in
the absence of DNA synthesis.
Vit B12 promotes conversion of methyl
tetrahydro folate (MTHF) to its active form.
In Vit B12 deficiency, MTHF accumulates
which leads to arrest of chromosomal
division.
Megaloblasts are produced in the BM
instead of normoblasts resulting in
Megaloblastic anemia.
45. DIETARY FACTORS
PROTEINS
Proteins are essential for the synthesis of globin
component of hemoglobin.
Protein deficiency is invariably associated with
hypochromic anemia.
OTHERS
Vit C helps in absorption of Iron. Vit C deficiency
hence leads to anemia.
Deficiency of copper and cobalt also leads to anemia.
46. APPLIED ASPECTS
IRON DEFICIENCY ANEMIA
microcytic, hypochromic anemia. Erythrocytes smaller than
normal and contain less hemoglobin than normal.
MEGALOBLASTIC ANEMIA
macrocytic anemia Abnormal erythrocytes precursors
(abnormally large erythrocytes) are found in the marrow.
47. OTHER FACTORS
ENVIRONMENTAL FACTORS
High altitude leads to hypoxia which
increases erythropoiesis.
DRUGS & CHEMICALS
Catecholamine, thyroxine, cobalt salts etc
influence erythropoiesis.
48. DYSERYTHROPOIESIS
Defective erythropoiesis is known
as dyserythropoiesis in which red blood cells
produced in the bone marrow are destroyed
before their release or have short life span after
entering circulation. Dyserythropoiesis
includes irregular nuclearity, budding nuclei,
multinuclearity, megaloblastoid changes, and
ring sideroblasts. Seen in megaloblastic anemia,
thalassemia, congenital dyserythropoietic
anemia etc.,
49. SUMMARY
The process of formation of Red Blood Cells is
called Erythropoiesis which is a part of
hematopoiesis.
Bone marrow becomes the sole site of
erythropoiesis in adults.
The stem cells give rise to normoblasts which
mature to reticulocytes and then erythrocytes.
The cytoplasm changes from basophilic to
acidophilic, the cell size decreases, nucleus
disappears and Hb appears.
There are several factors affecting erythropoiesis
and EPO plays a major role.
RBCs are destroyed in the spleen.