Enzymes are protein catalysts that speed up biochemical reactions without being consumed. They achieve this by lowering the activation energy of reactions. Enzymes are very specific and only catalyze one or a few reactions. The active site of an enzyme is where substrates bind and reactions occur. Many factors can influence an enzyme's activity, such as temperature, pH, substrate/product concentration, and inhibitors. Enzymes work by reducing the energy needed for reactions to occur and stabilizing the transition state.